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The map label for this gene is leuB [H]

Identifier: 103486574

GI number: 103486574

Start: 1114103

End: 1115149

Strand: Direct

Name: leuB [H]

Synonym: Sala_1085

Alternate gene names: 103486574

Gene position: 1114103-1115149 (Clockwise)

Preceding gene: 103486573

Following gene: 103486575

Centisome position: 33.3

GC content: 66.28

Gene sequence:

>1047_bases
TTGAATATCCTGTTGCTCGCTGGCGACGGCATCGGTCCGGAAATCATGGCGGAAGCCGAGAAGGTCATCGCGGCGCTCGC
ACTCCCCGTCGATCTGGATCGCGCGCTTGTGGGCGGTGCGGCCTGTGCGGCGACCGGCCATCCGCTGCCCGCGGAAACGC
TGGCGAAGGCGAAGGCCGCCGATGCGATCCTGTTCGGTGCCGTTGGCGACCCGCGCTTCGACAATGTGGAGCGCCATTTG
CGGCCCGAGCAGGCGATCCTTGGCCTGCGCGCCGAGCTTGGCCTGTTCGCCAACCTGCGCCCCGCAAAGCTGTTTTCCGG
GCTGGAGGATAGTTCGGCGCTGCGCCCCGAAGTCGCGTCGGCGATCGACCTGCTGATCGTCCGCGAGCTTAATGGCGACG
TCTATTTCGGCGAAAAAGGCACGCGCACGACCGCCAGCGGCGAACGGCAGGGCTATGACATCATGTCGTACAGCGAAAGC
GAGGTGCGGCGCATCGCGCATGTCGCGTTCAAGGCGGCCGCTGGACGGAACAAGCGGCTCTGCTCGGTCGACAAGGCGAA
TGTGCTCGAAACCTCGCAGCTGTGGCGCGACGTCGTGATCGAAGTGGCCGCCGACTATCCCGACGTGGCGCTTGCCCACA
TGTATGTCGACAATGCCGCGATGCAGCTGGTACGCAATCCGGGGCAGTTCGATGTCGTCGTCACCGGCAATCTGTTCGGC
GATATCCTTTCGGATCAGGCGTCGATGTGCGTCGGCTCGATCGGGCTGCTCGCGTCGGCGTCGCTGGGCGACGGCACGCG
GGGGCTGTATGAGCCGATCCACGGCAGCGCGCCCGACATTGCGGGGAAGGGCATTGCCAATCCGCTGGCGATGATCTTGT
CGCTTGCGATGCTGCTCCGCCATTCGGGGGGCGACGAGGCCAATGCGGCGCGGATCGAGGCAGCGGTGGCACAGATACTC
GCCGACGGCTGCCGCGGCGCCGACCTGGGCGGAACGATGGGCACGGCGGCCTTGGGCGATGCGGTCGTGGCGGCTTTGAA
CGGATAG

Upstream 100 bases:

>100_bases
GTCGAAGCACCGTCCCGTCACCGGACGGCGCCAGAAGAGAAGAGCGGCCCTTCGACATGTTCCGGGCGAACGGCCTTTTT
TGGACGGGAAGGGTGAACTG

Downstream 100 bases:

>100_bases
GGCAGATGAAAAAGACGACGGGTCTCGACCGGAACAAGACGAAGACGTGGCATCCCGCGACGCAGGCGGTGCGCGGCGGC
ACGTGGCGCAGCGAGCATGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNILLLAGDGIGPEIMAEAEKVIAALALPVDLDRALVGGAACAATGHPLPAETLAKAKAADAILFGAVGDPRFDNVERHL
RPEQAILGLRAELGLFANLRPAKLFSGLEDSSALRPEVASAIDLLIVRELNGDVYFGEKGTRTTASGERQGYDIMSYSES
EVRRIAHVAFKAAAGRNKRLCSVDKANVLETSQLWRDVVIEVAADYPDVALAHMYVDNAAMQLVRNPGQFDVVVTGNLFG
DILSDQASMCVGSIGLLASASLGDGTRGLYEPIHGSAPDIAGKGIANPLAMILSLAMLLRHSGGDEANAARIEAAVAQIL
ADGCRGADLGGTMGTAALGDAVVAALNG

Sequences:

>Translated_348_residues
MNILLLAGDGIGPEIMAEAEKVIAALALPVDLDRALVGGAACAATGHPLPAETLAKAKAADAILFGAVGDPRFDNVERHL
RPEQAILGLRAELGLFANLRPAKLFSGLEDSSALRPEVASAIDLLIVRELNGDVYFGEKGTRTTASGERQGYDIMSYSES
EVRRIAHVAFKAAAGRNKRLCSVDKANVLETSQLWRDVVIEVAADYPDVALAHMYVDNAAMQLVRNPGQFDVVVTGNLFG
DILSDQASMCVGSIGLLASASLGDGTRGLYEPIHGSAPDIAGKGIANPLAMILSLAMLLRHSGGDEANAARIEAAVAQIL
ADGCRGADLGGTMGTAALGDAVVAALNG
>Mature_348_residues
MNILLLAGDGIGPEIMAEAEKVIAALALPVDLDRALVGGAACAATGHPLPAETLAKAKAADAILFGAVGDPRFDNVERHL
RPEQAILGLRAELGLFANLRPAKLFSGLEDSSALRPEVASAIDLLIVRELNGDVYFGEKGTRTTASGERQGYDIMSYSES
EVRRIAHVAFKAAAGRNKRLCSVDKANVLETSQLWRDVVIEVAADYPDVALAHMYVDNAAMQLVRNPGQFDVVVTGNLFG
DILSDQASMCVGSIGLLASASLGDGTRGLYEPIHGSAPDIAGKGIANPLAMILSLAMLLRHSGGDEANAARIEAAVAQIL
ADGCRGADLGGTMGTAALGDAVVAALNG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=333, Percent_Identity=32.7327327327327, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI28178816, Length=350, Percent_Identity=29.7142857142857, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=29.0598290598291, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI4758582, Length=359, Percent_Identity=30.3621169916435, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI28178838, Length=309, Percent_Identity=32.0388349514563, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178819, Length=196, Percent_Identity=32.1428571428571, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI87081683, Length=350, Percent_Identity=48, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=35.5555555555556, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1787381, Length=399, Percent_Identity=26.0651629072682, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=33.5260115606936, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25144293, Length=350, Percent_Identity=30.8571428571429, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=353, Percent_Identity=29.1784702549575, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=30.2395209580838, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=356, Percent_Identity=43.8202247191011, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=37.292817679558, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=352, Percent_Identity=34.0909090909091, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=354, Percent_Identity=30.225988700565, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=32, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=32, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24661184, Length=355, Percent_Identity=30.4225352112676, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078633, Length=349, Percent_Identity=28.3667621776504, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24650122, Length=349, Percent_Identity=28.3667621776504, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078637, Length=349, Percent_Identity=28.3667621776504, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078635, Length=349, Percent_Identity=28.3667621776504, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI161078639, Length=347, Percent_Identity=28.5302593659942, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=29.7376093294461, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=29.7376093294461, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI20130355, Length=351, Percent_Identity=24.2165242165242, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36063; Mature: 36063

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLLAGDGIGPEIMAEAEKVIAALALPVDLDRALVGGAACAATGHPLPAETLAKAKAA
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCCHHHHHHHHHH
DAILFGAVGDPRFDNVERHLRPEQAILGLRAELGLFANLRPAKLFSGLEDSSALRPEVAS
CEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHH
AIDLLIVRELNGDVYFGEKGTRTTASGERQGYDIMSYSESEVRRIAHVAFKAAAGRNKRL
HHHHHHEEECCCCEEECCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCE
CSVDKANVLETSQLWRDVVIEVAADYPDVALAHMYVDNAAMQLVRNPGQFDVVVTGNLFG
ECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHH
DILSDQASMCVGSIGLLASASLGDGTRGLYEPIHGSAPDIAGKGIANPLAMILSLAMLLR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HSGGDEANAARIEAAVAQILADGCRGADLGGTMGTAALGDAVVAALNG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNILLLAGDGIGPEIMAEAEKVIAALALPVDLDRALVGGAACAATGHPLPAETLAKAKAA
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCCHHHHHHHHHH
DAILFGAVGDPRFDNVERHLRPEQAILGLRAELGLFANLRPAKLFSGLEDSSALRPEVAS
CEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHH
AIDLLIVRELNGDVYFGEKGTRTTASGERQGYDIMSYSESEVRRIAHVAFKAAAGRNKRL
HHHHHHEEECCCCEEECCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCE
CSVDKANVLETSQLWRDVVIEVAADYPDVALAHMYVDNAAMQLVRNPGQFDVVVTGNLFG
ECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHH
DILSDQASMCVGSIGLLASASLGDGTRGLYEPIHGSAPDIAGKGIANPLAMILSLAMLLR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HSGGDEANAARIEAAVAQILADGCRGADLGGTMGTAALGDAVVAALNG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA