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The map label for this gene is caiD [H]

Identifier: 103486431

GI number: 103486431

Start: 959681

End: 960454

Strand: Reverse

Name: caiD [H]

Synonym: Sala_0941

Alternate gene names: 103486431

Gene position: 960454-959681 (Counterclockwise)

Preceding gene: 103486433

Following gene: 103486426

Centisome position: 28.71

GC content: 67.44

Gene sequence:

>774_bases
ATGAGCGTTGCAATCGCAAAGGATGGGCCCGTCACCATCGTCACCATCGACCGGCCGAACAAACGCAACGCAGTCGATCC
GACGACCGCCGAGGCACTAAGGGACGCCTTCACCGCCTTTGCCGCCGACGAGGCGGCAAAGGTCGCGATCCTGACGGGTA
GTGCGGGCCATTTCTGCGCAGGTTTCGACCTTGGCGCGGTCGGCGCCAGCCGCCACGATCCCGACGGACCAGGTCCCATG
GGCCCGACGCGAATGTTGATGGACAAACCCGTGATCGCCGCGATCGAGGGCCATGCGGTGGCAGGTGGGCTCGAACTCGC
GCTCTGGTGCGACCTGCGCGTCGCTGCCGCTGGTGCCGTTTTCGGCGTCTATTGCCGCCGCTGGGGCGTCCCGCTGATCG
ATGGCGGCACCGTCCGCCTTCCGCGCATCGTTGGGCAGGGGCGCGCGCTCGACATGATCCTCACCGGTCGGCCGGTGGCC
GCCGACGAAGCGCAGCGCATCGGCCTTGCCGACCGGGTCGTTCCCGACGGCGACGCGCTGAGCGCCGCGATCGATCTCGC
GAAACATGTCGCTGCCTTCCCGCAAATCTGCATGAACAGCGACCGATTGAGCGCCTATCGCCAGTGGGACTTCGACATCG
AAGGCGCACTGGCGCACGAAGCCCGCGCAGGGGTCATCCCGCTCCGCGAGGGGGCAGCGGCGGGCGCGAGGCGATTTACC
GAAGGTGCAGGCCGCGGAGGCAGTTTTGCGCCTTTAAGGGAGACTGACTGCTAG

Upstream 100 bases:

>100_bases
AGAGGGGCCCGGCCCGCACCCCCTTTATCTAGGAGTCCGGCGCCCGGCTTGCAAGTCCCCCCGCCAAGCGCCACGCTGCC
GATGAACAGGAGGATGACCG

Downstream 100 bases:

>100_bases
GGTCAGGACCCCTCCACCGCGCTTCGATTGCTCCTCCGTGCCGGGGCGGAGCGGCAGCTTCCGGACGAAACCAGCCCCCA
GTCTATCGCTGCCCCATCAG

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSVAIAKDGPVTIVTIDRPNKRNAVDPTTAEALRDAFTAFAADEAAKVAILTGSAGHFCAGFDLGAVGASRHDPDGPGPM
GPTRMLMDKPVIAAIEGHAVAGGLELALWCDLRVAAAGAVFGVYCRRWGVPLIDGGTVRLPRIVGQGRALDMILTGRPVA
ADEAQRIGLADRVVPDGDALSAAIDLAKHVAAFPQICMNSDRLSAYRQWDFDIEGALAHEARAGVIPLREGAAAGARRFT
EGAGRGGSFAPLRETDC

Sequences:

>Translated_257_residues
MSVAIAKDGPVTIVTIDRPNKRNAVDPTTAEALRDAFTAFAADEAAKVAILTGSAGHFCAGFDLGAVGASRHDPDGPGPM
GPTRMLMDKPVIAAIEGHAVAGGLELALWCDLRVAAAGAVFGVYCRRWGVPLIDGGTVRLPRIVGQGRALDMILTGRPVA
ADEAQRIGLADRVVPDGDALSAAIDLAKHVAAFPQICMNSDRLSAYRQWDFDIEGALAHEARAGVIPLREGAAAGARRFT
EGAGRGGSFAPLRETDC
>Mature_256_residues
SVAIAKDGPVTIVTIDRPNKRNAVDPTTAEALRDAFTAFAADEAAKVAILTGSAGHFCAGFDLGAVGASRHDPDGPGPMG
PTRMLMDKPVIAAIEGHAVAGGLELALWCDLRVAAAGAVFGVYCRRWGVPLIDGGTVRLPRIVGQGRALDMILTGRPVAA
DEAQRIGLADRVVPDGDALSAAIDLAKHVAAFPQICMNSDRLSAYRQWDFDIEGALAHEARAGVIPLREGAAAGARRFTE
GAGRGGSFAPLRETDC

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=35.9116022099448, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI194097323, Length=193, Percent_Identity=32.6424870466321, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=30.4347826086957, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI31542718, Length=233, Percent_Identity=26.1802575107296, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4502327, Length=233, Percent_Identity=30.9012875536481, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI221142681, Length=232, Percent_Identity=34.9137931034483, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787659, Length=223, Percent_Identity=31.390134529148, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=34.0425531914894, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788597, Length=178, Percent_Identity=32.5842696629214, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787660, Length=196, Percent_Identity=32.1428571428571, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=31.25, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=48.1632653061224, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=234, Percent_Identity=28.6324786324786, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=215, Percent_Identity=29.3023255813954, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=228, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=29.3859649122807, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=194, Percent_Identity=32.4742268041237, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=194, Percent_Identity=32.4742268041237, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=211, Percent_Identity=27.9620853080569, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=199, Percent_Identity=32.1608040201005, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=199, Percent_Identity=28.1407035175879, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=209, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21357171, Length=227, Percent_Identity=44.9339207048458, Blast_Score=203, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=45.4976303317536, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI20129971, Length=220, Percent_Identity=30.9090909090909, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653477, Length=220, Percent_Identity=30.9090909090909, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=28.8, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583077, Length=189, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583079, Length=189, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921000, Length=189, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920382, Length=238, Percent_Identity=26.890756302521, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 26705; Mature: 26574

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIAKDGPVTIVTIDRPNKRNAVDPTTAEALRDAFTAFAADEAAKVAILTGSAGHFCA
CEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
GFDLGAVGASRHDPDGPGPMGPTRMLMDKPVIAAIEGHAVAGGLELALWCDLRVAAAGAV
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCEEECCEEEEEEECCHHHHHHHH
FGVYCRRWGVPLIDGGTVRLPRIVGQGRALDMILTGRPVAADEAQRIGLADRVVPDGDAL
HHHHHHHCCCCEECCCCEEECHHHCCCCEEEEEEECCCCCCCHHHHCCCHHCCCCCCHHH
SAAIDLAKHVAAFPQICMNSDRLSAYRQWDFDIEGALAHEARAGVIPLREGAAAGARRFT
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHCCCCEECCCCCCHHHHHHH
EGAGRGGSFAPLRETDC
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVAIAKDGPVTIVTIDRPNKRNAVDPTTAEALRDAFTAFAADEAAKVAILTGSAGHFCA
EEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEE
GFDLGAVGASRHDPDGPGPMGPTRMLMDKPVIAAIEGHAVAGGLELALWCDLRVAAAGAV
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCEEECCEEEEEEECCHHHHHHHH
FGVYCRRWGVPLIDGGTVRLPRIVGQGRALDMILTGRPVAADEAQRIGLADRVVPDGDAL
HHHHHHHCCCCEECCCCEEECHHHCCCCEEEEEEECCCCCCCHHHHCCCHHCCCCCCHHH
SAAIDLAKHVAAFPQICMNSDRLSAYRQWDFDIEGALAHEARAGVIPLREGAAAGARRFT
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHCCCCEECCCCCCHHHHHHH
EGAGRGGSFAPLRETDC
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA