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The map label for this gene is sir1 [H]

Identifier: 103486262

GI number: 103486262

Start: 776486

End: 778117

Strand: Reverse

Name: sir1 [H]

Synonym: Sala_0770

Alternate gene names: 103486262

Gene position: 778117-776486 (Counterclockwise)

Preceding gene: 103486263

Following gene: 103486261

Centisome position: 23.26

GC content: 63.3

Gene sequence:

>1632_bases
ATGTATAAATATGACGAATATGATCAGGCGATGGTCGAGGCGCGCGTCGCCGAGTTCAGCGACCAGGTGCGCCGCCGCCT
CGCGGGCGAAATCACCGAGGACCAGTTCAAGCCGCTGCGGCTGATGAACGGCCTCTATCTCCAGCTCCACGCCTATATGC
TGCGCGTCGCGGTGCCCTACGGGACGCTCGACAGCCGGCAGATGCGGATGCTCGCGCACATCGCGCGCACATATGACCGC
GATTACGGCCATTTCACCACGCGCCAGAATATCCAGTACAACTGGATCAAGCTGGAGGATGCCCCCGCGATCCTCGCCGA
TCTGGCGTCGGTCGAAATGCACGCGATCCAGACAAGCGGCAATTGCATCCGCAACATCAGCTCGGACCAGTGGGCCGGGG
CCGCCGCTGACGAGATCACCGATCCGCGTCCATGGGCCGAATTGCTGCGCCAATGGTCGAGCTTTCATCCGGAATTCAGC
TATCTGCCGCGCAAGTTCAAGATCGCCGTGATCGCCGCCGACGAAGACCGCGCCGCGATGCGCCTCCACGACATCGGCAT
CCAGATCGTCGAACGCGATGGCGTTCACGGCGCCGCCTTCTACGTCGGCGGCGGCATGGGCCGCACGCCGATGATTGCGC
CGCTGATCAAGGATTTTGTGCCGCTCGACGATCTGCTCAGCTACGCCGAGGCGTGCCTGCGCGTGTACAACCGCTATGGC
CGCCGCGACAATATCTACAAGGCGCGGATCAAGATCCTGATCCACGAACTGGGCGCCGACGAATATCGCCGCCAGGTCGA
GGAAGAGTTCGCGCATGTGAAATCGCTCGGCATCGACCCGCCAAAGGCCGAGTTCGACCGCATCGCCGCGCAGTTCGCGC
CGCCACCGCTCGACGCATCGGCGCCCGACGCGATCGACCGCAGCGATCCCGATTTCGCGGTCTGGGTCGACCAGAATGTG
GCGGCGCACAAGGTGCCCGGCCATGCGATCGTCAACATCAGCCTGAAGCCCGTCGGCGGCATCCCCGGTGACGCCAGTTC
GGCGCAGATCGACCTGATGGCCGATCTGGCCGAACGCTATAGCCAGGACGAGTTGCGCGTCACCCACGCGCAGAACATCG
TGCTTCCGCACGTCCGCAAGGCCGACCTTTACGCCATCTGGCAAAAGCTCGACGAAGCGGGGCTTGCGACACCCAACCTC
GACCTCATCAGCGACATCATCGCCTGCCCCGGCCTCGATTATTGCAGCCTTGCCAATGCGCGCTCGATCCCGGTCGCGCA
GAAGATCGCGATGCGCTTTGCCGATCTTGGCCGTCAGAAGGAACTGGGCGAATTGAAGCTCAAGATTTCGGGCTGCATCA
ATGCCTGCGGCCACCATCACGCCGGCCACATCGGCATCCTCGGCGTCGATCGCAAGGGCACCGAAAACTACCAGCTGCTG
CTTGGCGGATCGGGCGCAGAGGATGTGTCGCTCGCCACGATCACCGGCCCCGGTTTCGACGAGGACGGCATCGTCGATGC
GATCGAGCGCGTCACCGACAAATATCTCGCGACAAGGACCGAGGGCGAACGTTTCGTCGACACCTTCCGCCGCGTCGGCA
TGGCCCCGTTCAAGGAGGCGATCTATGGTTGA

Upstream 100 bases:

>100_bases
GCGCATATCAAGGACCGCATCCGCGCGTTGGGGCCGACCGTGCGCCCCGACCTGACATTGAAACCCGCCGATCCGACGGC
CGGCGACTGGGTGATCTGAC

Downstream 100 bases:

>100_bases
ACGCTCCGATAATCAGTCCCTTCCGCAAGCGGAAGGGTGGCACAGCGACGGCGAGGAACCCTGCGTCACGCTCGACGCCT
TCCTCGCGCAGTCGAATGCC

Product: nitrite/sulfite reductase, hemoprotein beta-component, ferrodoxin-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MYKYDEYDQAMVEARVAEFSDQVRRRLAGEITEDQFKPLRLMNGLYLQLHAYMLRVAVPYGTLDSRQMRMLAHIARTYDR
DYGHFTTRQNIQYNWIKLEDAPAILADLASVEMHAIQTSGNCIRNISSDQWAGAAADEITDPRPWAELLRQWSSFHPEFS
YLPRKFKIAVIAADEDRAAMRLHDIGIQIVERDGVHGAAFYVGGGMGRTPMIAPLIKDFVPLDDLLSYAEACLRVYNRYG
RRDNIYKARIKILIHELGADEYRRQVEEEFAHVKSLGIDPPKAEFDRIAAQFAPPPLDASAPDAIDRSDPDFAVWVDQNV
AAHKVPGHAIVNISLKPVGGIPGDASSAQIDLMADLAERYSQDELRVTHAQNIVLPHVRKADLYAIWQKLDEAGLATPNL
DLISDIIACPGLDYCSLANARSIPVAQKIAMRFADLGRQKELGELKLKISGCINACGHHHAGHIGILGVDRKGTENYQLL
LGGSGAEDVSLATITGPGFDEDGIVDAIERVTDKYLATRTEGERFVDTFRRVGMAPFKEAIYG

Sequences:

>Translated_543_residues
MYKYDEYDQAMVEARVAEFSDQVRRRLAGEITEDQFKPLRLMNGLYLQLHAYMLRVAVPYGTLDSRQMRMLAHIARTYDR
DYGHFTTRQNIQYNWIKLEDAPAILADLASVEMHAIQTSGNCIRNISSDQWAGAAADEITDPRPWAELLRQWSSFHPEFS
YLPRKFKIAVIAADEDRAAMRLHDIGIQIVERDGVHGAAFYVGGGMGRTPMIAPLIKDFVPLDDLLSYAEACLRVYNRYG
RRDNIYKARIKILIHELGADEYRRQVEEEFAHVKSLGIDPPKAEFDRIAAQFAPPPLDASAPDAIDRSDPDFAVWVDQNV
AAHKVPGHAIVNISLKPVGGIPGDASSAQIDLMADLAERYSQDELRVTHAQNIVLPHVRKADLYAIWQKLDEAGLATPNL
DLISDIIACPGLDYCSLANARSIPVAQKIAMRFADLGRQKELGELKLKISGCINACGHHHAGHIGILGVDRKGTENYQLL
LGGSGAEDVSLATITGPGFDEDGIVDAIERVTDKYLATRTEGERFVDTFRRVGMAPFKEAIYG
>Mature_543_residues
MYKYDEYDQAMVEARVAEFSDQVRRRLAGEITEDQFKPLRLMNGLYLQLHAYMLRVAVPYGTLDSRQMRMLAHIARTYDR
DYGHFTTRQNIQYNWIKLEDAPAILADLASVEMHAIQTSGNCIRNISSDQWAGAAADEITDPRPWAELLRQWSSFHPEFS
YLPRKFKIAVIAADEDRAAMRLHDIGIQIVERDGVHGAAFYVGGGMGRTPMIAPLIKDFVPLDDLLSYAEACLRVYNRYG
RRDNIYKARIKILIHELGADEYRRQVEEEFAHVKSLGIDPPKAEFDRIAAQFAPPPLDASAPDAIDRSDPDFAVWVDQNV
AAHKVPGHAIVNISLKPVGGIPGDASSAQIDLMADLAERYSQDELRVTHAQNIVLPHVRKADLYAIWQKLDEAGLATPNL
DLISDIIACPGLDYCSLANARSIPVAQKIAMRFADLGRQKELGELKLKISGCINACGHHHAGHIGILGVDRKGTENYQLL
LGGSGAEDVSLATITGPGFDEDGIVDAIERVTDKYLATRTEGERFVDTFRRVGMAPFKEAIYG

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=523, Percent_Identity=23.1357552581262, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 60545; Mature: 60545

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKYDEYDQAMVEARVAEFSDQVRRRLAGEITEDQFKPLRLMNGLYLQLHAYMLRVAVPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTLDSRQMRMLAHIARTYDRDYGHFTTRQNIQYNWIKLEDAPAILADLASVEMHAIQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHEEECCC
NCIRNISSDQWAGAAADEITDPRPWAELLRQWSSFHPEFSYLPRKFKIAVIAADEDRAAM
HHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCHHHCCCEEEEEEEEECCCCHHH
RLHDIGIQIVERDGVHGAAFYVGGGMGRTPMIAPLIKDFVPLDDLLSYAEACLRVYNRYG
HHHHCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RRDNIYKARIKILIHELGADEYRRQVEEEFAHVKSLGIDPPKAEFDRIAAQFAPPPLDAS
CCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
APDAIDRSDPDFAVWVDQNVAAHKVPGHAIVNISLKPVGGIPGDASSAQIDLMADLAERY
CCCCCCCCCCCEEEEEECCCCEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHC
SQDELRVTHAQNIVLPHVRKADLYAIWQKLDEAGLATPNLDLISDIIACPGLDYCSLANA
CCCCEEEEHHCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCCC
RSIPVAQKIAMRFADLGRQKELGELKLKISGCINACGHHHAGHIGILGVDRKGTENYQLL
CCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEE
LGGSGAEDVSLATITGPGFDEDGIVDAIERVTDKYLATRTEGERFVDTFRRVGMAPFKEA
EECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
IYG
HCC
>Mature Secondary Structure
MYKYDEYDQAMVEARVAEFSDQVRRRLAGEITEDQFKPLRLMNGLYLQLHAYMLRVAVPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTLDSRQMRMLAHIARTYDRDYGHFTTRQNIQYNWIKLEDAPAILADLASVEMHAIQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHEEECCC
NCIRNISSDQWAGAAADEITDPRPWAELLRQWSSFHPEFSYLPRKFKIAVIAADEDRAAM
HHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCCCCHHHCCCEEEEEEEEECCCCHHH
RLHDIGIQIVERDGVHGAAFYVGGGMGRTPMIAPLIKDFVPLDDLLSYAEACLRVYNRYG
HHHHCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RRDNIYKARIKILIHELGADEYRRQVEEEFAHVKSLGIDPPKAEFDRIAAQFAPPPLDAS
CCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
APDAIDRSDPDFAVWVDQNVAAHKVPGHAIVNISLKPVGGIPGDASSAQIDLMADLAERY
CCCCCCCCCCCEEEEEECCCCEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHC
SQDELRVTHAQNIVLPHVRKADLYAIWQKLDEAGLATPNLDLISDIIACPGLDYCSLANA
CCCCEEEEHHCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCCC
RSIPVAQKIAMRFADLGRQKELGELKLKISGCINACGHHHAGHIGILGVDRKGTENYQLL
CCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEE
LGGSGAEDVSLATITGPGFDEDGIVDAIERVTDKYLATRTEGERFVDTFRRVGMAPFKEA
EECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHH
IYG
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA