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The map label for this gene is fadI [H]

Identifier: 103485607

GI number: 103485607

Start: 108571

End: 109836

Strand: Direct

Name: fadI [H]

Synonym: Sala_0110

Alternate gene names: 103485607

Gene position: 108571-109836 (Clockwise)

Preceding gene: 103485606

Following gene: 103485608

Centisome position: 3.25

GC content: 63.98

Gene sequence:

>1266_bases
ATGAGCCGCTTTGCTGAACCCGTCTTTGTTGCCGCCGGCCTTCGCACTCCCTTCGGCCGCGGCGGAGGGGCTTTGGCTGC
TTATGATGCCGTCTCTTTGTCCGTCCCTCTTGTGCAGGCGATGGCGGCGCAAGCAGAGCCGGATCTTCTCATCTGGGGTA
CCGTGATCCCGAATTTGGGTTGGAGCAACATTGCTCGCGAGACCTGGCTTGACGCCAAGCTCAATCCGAATGTCCCCGCA
TTTTCGGTCGTTTTGGCATGCTCTACCAGCATGACGGCGACTTTCGCTGCGGCAGGGATGCTGGGCGGAGGAACCGATCT
CACGATGGTGGGCGGTGCCGAAGTCATGAGCCGACCTCCCATCGCTTTGACGGCCGATGCATCGAAGCGGCTCACCGACC
TCTTTGCGCAAGATCCAGCGGCGGCGCTTGCGGCCCTCCAGTCTCTTGCCCTCCACGACTATGTGCTCCCGACCAAGGGA
TGGGCCAACCGGATCACCGGCAGGACAATGGGCGATCATATGGAGGACACCGCCAAGGCCTGGCGGATCTCGCGCGAGGC
GCAGGACGATTGGGCGCTAAAGAGCCACCAGCGGTCGGTCGCTGGTTGGGAGCGAGGTTTCTTCGACGATCTCGCGATTT
CCCTTCCTGAACTGGCGCGCGATGCGAACCCCCGCGCCGATACGTCGCCCGAACGGCTTGCCGCACTCAAGCCCGTCTTC
GATCGCGACAGTGGCAGGGGAACGCTGACTGCCGGCAACAGCTCGCCGATAACGGATGGCGCCGCCGGTTGCTGGGTGGC
AAACGAAGCCGGCGTGGCACGACTGCCGGCGGGCACCCCCTACGCGCGGCTCATCGATTACGAGGTGTCGGCCGTCGATT
TCCACACCGAGGGTCTGTTGATGGCACCCTCCTATGGCATTCCACGTCTGCTCGCGCGGCATCAACTAAGCTTCTCCGAT
ATCGCTCTTTGGGAAATCCACGAAGCCTTCGCCGCCCAGGTCCTGGCGAATGTCGCGGCCATCACCGACCGGGAGTGGGT
GCGCGAAACCGCCGGTGTCCAGTCGGATTTGGGCGACTTCGACTGGGACCGCGTCAATCCCAACGGGGGCTCGGTAGCAA
TCGGTCATCCCTTCGGCGCGACAGGCGCGCGCGACCTCAGCCAGGCAGTGAAGGAGCTCTGGGACATGCCGAGCGGATCA
CGGGCAATCGTCAGCATCTGCGCCGACGGCGGCCAAGGCACCGTCGCACTCCTCGAACGCCCCTGA

Upstream 100 bases:

>100_bases
GTCGACCCCTCGATAATCAAGACCGAGCGCTATGGTGGTTAGCGGCGCATACTCCGTCGCGCCCCACCTTTCACCCTGCG
GATAATATTTGGAGATCGTC

Downstream 100 bases:

>100_bases
TTCACGGGCCGCTGCGAACGCCGTGCCATTGACAATATGAGAGGATATTCCGTTGGTCTCAGCAGTTTGGAACGACACCA
CCATCGCCGCAAGCGACGAG

Product: Acetyl-CoA C-acyltransferase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSRFAEPVFVAAGLRTPFGRGGGALAAYDAVSLSVPLVQAMAAQAEPDLLIWGTVIPNLGWSNIARETWLDAKLNPNVPA
FSVVLACSTSMTATFAAAGMLGGGTDLTMVGGAEVMSRPPIALTADASKRLTDLFAQDPAAALAALQSLALHDYVLPTKG
WANRITGRTMGDHMEDTAKAWRISREAQDDWALKSHQRSVAGWERGFFDDLAISLPELARDANPRADTSPERLAALKPVF
DRDSGRGTLTAGNSSPITDGAAGCWVANEAGVARLPAGTPYARLIDYEVSAVDFHTEGLLMAPSYGIPRLLARHQLSFSD
IALWEIHEAFAAQVLANVAAITDREWVRETAGVQSDLGDFDWDRVNPNGGSVAIGHPFGATGARDLSQAVKELWDMPSGS
RAIVSICADGGQGTVALLERP

Sequences:

>Translated_421_residues
MSRFAEPVFVAAGLRTPFGRGGGALAAYDAVSLSVPLVQAMAAQAEPDLLIWGTVIPNLGWSNIARETWLDAKLNPNVPA
FSVVLACSTSMTATFAAAGMLGGGTDLTMVGGAEVMSRPPIALTADASKRLTDLFAQDPAAALAALQSLALHDYVLPTKG
WANRITGRTMGDHMEDTAKAWRISREAQDDWALKSHQRSVAGWERGFFDDLAISLPELARDANPRADTSPERLAALKPVF
DRDSGRGTLTAGNSSPITDGAAGCWVANEAGVARLPAGTPYARLIDYEVSAVDFHTEGLLMAPSYGIPRLLARHQLSFSD
IALWEIHEAFAAQVLANVAAITDREWVRETAGVQSDLGDFDWDRVNPNGGSVAIGHPFGATGARDLSQAVKELWDMPSGS
RAIVSICADGGQGTVALLERP
>Mature_420_residues
SRFAEPVFVAAGLRTPFGRGGGALAAYDAVSLSVPLVQAMAAQAEPDLLIWGTVIPNLGWSNIARETWLDAKLNPNVPAF
SVVLACSTSMTATFAAAGMLGGGTDLTMVGGAEVMSRPPIALTADASKRLTDLFAQDPAAALAALQSLALHDYVLPTKGW
ANRITGRTMGDHMEDTAKAWRISREAQDDWALKSHQRSVAGWERGFFDDLAISLPELARDANPRADTSPERLAALKPVFD
RDSGRGTLTAGNSSPITDGAAGCWVANEAGVARLPAGTPYARLIDYEVSAVDFHTEGLLMAPSYGIPRLLARHQLSFSDI
ALWEIHEAFAAQVLANVAAITDREWVRETAGVQSDLGDFDWDRVNPNGGSVAIGHPFGATGARDLSQAVKELWDMPSGSR
AIVSICADGGQGTVALLERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=32.9411764705882, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI167614485, Length=427, Percent_Identity=30.4449648711944, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4557237, Length=439, Percent_Identity=28.9293849658314, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI148539872, Length=435, Percent_Identity=28.0459770114943, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI4501853, Length=389, Percent_Identity=29.0488431876607, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1788683, Length=406, Percent_Identity=34.7290640394089, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788554, Length=430, Percent_Identity=32.7906976744186, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787663, Length=441, Percent_Identity=31.5192743764172, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082165, Length=429, Percent_Identity=31.2354312354312, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI48994986, Length=435, Percent_Identity=31.0344827586207, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=32.7830188679245, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17535921, Length=436, Percent_Identity=29.8165137614679, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI133906874, Length=438, Percent_Identity=30.5936073059361, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=425, Percent_Identity=27.7647058823529, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17535917, Length=166, Percent_Identity=32.5301204819277, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322031, Length=256, Percent_Identity=35.15625, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=437, Percent_Identity=27.9176201372998, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17137578, Length=431, Percent_Identity=34.8027842227378, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24640423, Length=445, Percent_Identity=31.4606741573034, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24655093, Length=427, Percent_Identity=28.1030444964871, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI17648125, Length=435, Percent_Identity=28.5057471264368, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 44590; Mature: 44458

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFAEPVFVAAGLRTPFGRGGGALAAYDAVSLSVPLVQAMAAQAEPDLLIWGTVIPNLG
CCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
WSNIARETWLDAKLNPNVPAFSVVLACSTSMTATFAAAGMLGGGTDLTMVGGAEVMSRPP
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCCC
IALTADASKRLTDLFAQDPAAALAALQSLALHDYVLPTKGWANRITGRTMGDHMEDTAKA
EEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCHHHHHHHHH
WRISREAQDDWALKSHQRSVAGWERGFFDDLAISLPELARDANPRADTSPERLAALKPVF
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHH
DRDSGRGTLTAGNSSPITDGAAGCWVANEAGVARLPAGTPYARLIDYEVSAVDFHTEGLL
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCCEECCCCCCCHHEEEEEEEEEEEECCCEE
MAPSYGIPRLLARHQLSFSDIALWEIHEAFAAQVLANVAAITDREWVRETAGVQSDLGDF
ECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
DWDRVNPNGGSVAIGHPFGATGARDLSQAVKELWDMPSGSRAIVSICADGGQGTVALLER
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEC
P
C
>Mature Secondary Structure 
SRFAEPVFVAAGLRTPFGRGGGALAAYDAVSLSVPLVQAMAAQAEPDLLIWGTVIPNLG
CCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
WSNIARETWLDAKLNPNVPAFSVVLACSTSMTATFAAAGMLGGGTDLTMVGGAEVMSRPP
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCCC
IALTADASKRLTDLFAQDPAAALAALQSLALHDYVLPTKGWANRITGRTMGDHMEDTAKA
EEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCHHHHHHHHH
WRISREAQDDWALKSHQRSVAGWERGFFDDLAISLPELARDANPRADTSPERLAALKPVF
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHH
DRDSGRGTLTAGNSSPITDGAAGCWVANEAGVARLPAGTPYARLIDYEVSAVDFHTEGLL
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCCEECCCCCCCHHEEEEEEEEEEEECCCEE
MAPSYGIPRLLARHQLSFSDIALWEIHEAFAAQVLANVAAITDREWVRETAGVQSDLGDF
ECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
DWDRVNPNGGSVAIGHPFGATGARDLSQAVKELWDMPSGSRAIVSICADGGQGTVALLER
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA