Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 103485533

GI number: 103485533

Start: 33976

End: 34914

Strand: Direct

Name: metF [H]

Synonym: Sala_0035

Alternate gene names: 103485533

Gene position: 33976-34914 (Clockwise)

Preceding gene: 103485532

Following gene: 103485534

Centisome position: 1.02

GC content: 67.09

Gene sequence:

>939_bases
ATGACCGCGAATCTCGACGCGCTGGCGGAAGCGCGCCGCGCGGCGGCGTCGCCGTTGTTCGCCAATCTCGACGGCGACGT
CGGCGTCAGCTTCGAGTTTTTCCCGCCGAAGTCGGAGAAGATGGAGGCGCAGCTGTGGGATACGTTCCGCACGCTCGAAC
CGCTGGCCCCCAGCTTCGTCTCGGTCACCTATGGCGCCGGTGGGTCGACCCGCGAGCGCACCCATGCCACGGTCGCGCGC
ATCGCGGCGGAGAGCCGGGTGCCGCCCGCGGCGCACCTTACCTGTGTCGAAGCCTCGCGCGCCGAAATCGACGAGGTGGC
GCGCGCCTATTGGGATGCGGGCGTGCGCCATATCGTCGCGCTGCGCGGCGACGTTCCGGGCGGCGGACCCTATCGCGCGC
ACCCCGACGGCTATGCCAATGCGGTCGAACTCGTCGCGGGGTTGAAGGCGATCGCGCCGTTCGACATCTCGGTCGCCGCC
TATCCCGAAGTGCATCCCGATGCCGATTGTCCACAGTCCGACCTCGACAATCTGAAGCGCAAGCTCGACGCGGGTGCAAA
CCGGGCAATCACGCAATTCTTCTTTTCGCCCGACAGTTTCCTCCGCTTTCGCGACACCGCGGCGGCGGCGGGAATCGACG
CGCCGATCATCCCCGGTATCCTGCCCGTGTCGAACGTGTCGCAGACGCGGCGGATGGCGGACATGTGCGGTACCGCGATC
CCCGCGTGGATGGTGTCGCTGTTCGATGGGCTCGACGACCACCCGGCGGCGCGCCAGCTCGTCGCGGCGACGGTCGCCGC
CGAAATGTGCCGCCGCCTCTATGCAGGGGGGGTGCGCGATTTCCATTTCTACACGCTGAACCGCGCCGAACTCAGCTATG
CCATTTGTCATTTGCTGGGGCTGCGGCCAGTATCGACATTGAAGGATCGGGCAGCATGA

Upstream 100 bases:

>100_bases
AAGGCGGGAAGCTGGCCGTCAAGCTCTGGCTCGGACGTCGCCGGAGCGACCAGGATCAACCCCCCGTCAGCGACGGCGGA
CCGACGAAAAGGCTTGCTGC

Downstream 100 bases:

>100_bases
CATCGTCATTGGCCAGCAGCGCGCGCGACGCGCTGAAAGAGGCGGCGAAGCAGCGCATCCTCATCAAGGATGGGCCCTTC
GGTACCGCCTTTCAGCATCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTANLDALAEARRAAASPLFANLDGDVGVSFEFFPPKSEKMEAQLWDTFRTLEPLAPSFVSVTYGAGGSTRERTHATVAR
IAAESRVPPAAHLTCVEASRAEIDEVARAYWDAGVRHIVALRGDVPGGGPYRAHPDGYANAVELVAGLKAIAPFDISVAA
YPEVHPDADCPQSDLDNLKRKLDAGANRAITQFFFSPDSFLRFRDTAAAAGIDAPIIPGILPVSNVSQTRRMADMCGTAI
PAWMVSLFDGLDDHPAARQLVAATVAAEMCRRLYAGGVRDFHFYTLNRAELSYAICHLLGLRPVSTLKDRAA

Sequences:

>Translated_312_residues
MTANLDALAEARRAAASPLFANLDGDVGVSFEFFPPKSEKMEAQLWDTFRTLEPLAPSFVSVTYGAGGSTRERTHATVAR
IAAESRVPPAAHLTCVEASRAEIDEVARAYWDAGVRHIVALRGDVPGGGPYRAHPDGYANAVELVAGLKAIAPFDISVAA
YPEVHPDADCPQSDLDNLKRKLDAGANRAITQFFFSPDSFLRFRDTAAAAGIDAPIIPGILPVSNVSQTRRMADMCGTAI
PAWMVSLFDGLDDHPAARQLVAATVAAEMCRRLYAGGVRDFHFYTLNRAELSYAICHLLGLRPVSTLKDRAA
>Mature_311_residues
TANLDALAEARRAAASPLFANLDGDVGVSFEFFPPKSEKMEAQLWDTFRTLEPLAPSFVSVTYGAGGSTRERTHATVARI
AAESRVPPAAHLTCVEASRAEIDEVARAYWDAGVRHIVALRGDVPGGGPYRAHPDGYANAVELVAGLKAIAPFDISVAAY
PEVHPDADCPQSDLDNLKRKLDAGANRAITQFFFSPDSFLRFRDTAAAAGIDAPIIPGILPVSNVSQTRRMADMCGTAIP
AWMVSLFDGLDDHPAARQLVAATVAAEMCRRLYAGGVRDFHFYTLNRAELSYAICHLLGLRPVSTLKDRAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=284, Percent_Identity=32.7464788732394, Blast_Score=151, Evalue=6e-37,
Organism=Escherichia coli, GI1790377, Length=293, Percent_Identity=55.6313993174061, Blast_Score=340, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI25147436, Length=280, Percent_Identity=31.0714285714286, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=280, Percent_Identity=31.0714285714286, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=296, Percent_Identity=33.7837837837838, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321313, Length=311, Percent_Identity=28.9389067524116, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24662658, Length=291, Percent_Identity=26.1168384879725, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33554; Mature: 33423

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANLDALAEARRAAASPLFANLDGDVGVSFEFFPPKSEKMEAQLWDTFRTLEPLAPSFV
CCCCHHHHHHHHHHHCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCE
SVTYGAGGSTRERTHATVARIAAESRVPPAAHLTCVEASRAEIDEVARAYWDAGVRHIVA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHEEEE
LRGDVPGGGPYRAHPDGYANAVELVAGLKAIAPFDISVAAYPEVHPDADCPQSDLDNLKR
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHH
KLDAGANRAITQFFFSPDSFLRFRDTAAAAGIDAPIIPGILPVSNVSQTRRMADMCGTAI
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PAWMVSLFDGLDDHPAARQLVAATVAAEMCRRLYAGGVRDFHFYTLNRAELSYAICHLLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHC
LRPVSTLKDRAA
CCCHHHHHHCCC
>Mature Secondary Structure 
TANLDALAEARRAAASPLFANLDGDVGVSFEFFPPKSEKMEAQLWDTFRTLEPLAPSFV
CCCHHHHHHHHHHHCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCE
SVTYGAGGSTRERTHATVARIAAESRVPPAAHLTCVEASRAEIDEVARAYWDAGVRHIVA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHEEEE
LRGDVPGGGPYRAHPDGYANAVELVAGLKAIAPFDISVAAYPEVHPDADCPQSDLDNLKR
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHH
KLDAGANRAITQFFFSPDSFLRFRDTAAAAGIDAPIIPGILPVSNVSQTRRMADMCGTAI
HHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PAWMVSLFDGLDDHPAARQLVAATVAAEMCRRLYAGGVRDFHFYTLNRAELSYAICHLLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHC
LRPVSTLKDRAA
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA