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The map label for this gene is metH [H]

Identifier: 103485534

GI number: 103485534

Start: 34911

End: 35978

Strand: Direct

Name: metH [H]

Synonym: Sala_0036

Alternate gene names: 103485534

Gene position: 34911-35978 (Clockwise)

Preceding gene: 103485533

Following gene: 103485535

Centisome position: 1.04

GC content: 64.7

Gene sequence:

>1068_bases
ATGACATCGTCATTGGCCAGCAGCGCGCGCGACGCGCTGAAAGAGGCGGCGAAGCAGCGCATCCTCATCAAGGATGGGCC
CTTCGGTACCGCCTTTCAGCATCGCAACGCCTATGTCGGCGTCGACGGGGCCGAACACGGCCTGTCGATCGATCCGCGCG
GCAACGGCGATTATCTCAACCTGGCGCGGCCCGATATCGTTGCGGACATCATGCGCGACTATCTTGTCGCGGGCGCCGAT
ATTATCGCAACCAACACCTTCAGCGCGAATCGCATCAGCCAGGCCGATTATGGCGCCGAGCATCTGGTGGCCGGGATCAA
TCTGGCCAGTGCGCGCCTCGCCCGCCGCGAGGCCGATGCGATGCAGCAAAAGGACGGCCGTCGCCGCTTCGTCGCTGGCG
CGGTCGGGCCGACGAACAAGACGCTGTCGCTGTCGCCCGACGTCAACAATCCGGGTTTTCGCGAGATCGACTTCGACGAA
TTGAAGGACGTCTATCTGGAACAGGTGGTCGCGCTGGCCGAAGGCGGCGCCGATTTCATCCTGATCGAGACCATCTTCGA
CACGCTGAATGCGAAAGCCGGGATCGCCGCGACGCTGGAAGCCGAAGCGGCGTTGGGGCGCGAACTGCCGCTGATGATCT
CGATGACCCTGACCGACCTGTCGGGCCGCAATCTGTCGGGGCACACGGTCGAGGCCTTCTGGTATGCGGTGCGTCATGCA
AAACCGATGACGATCGGGCTCAATTGCTCGTTCGGCGCGGCGCAGCTGCGCCCGCATGTTAAGGTTCTGTCGGACATCGC
CGATGCGCTGGTGATGGTCTATCCCAATGCGGGCTTGCCGAACGAGCTTGGCGAATATGACGAGCTGCCCGAGACGACAG
CCGAACTCGTCCGCGAATGGGCCGAGCATGGGCAGGTCAATATCCTCGGCGGCTGCTGCGGTTCGACGCCCGACCATATC
GCGGCGATGGCGAAGGCGGTCGAGGGGCTGGCGCCGCGCCAGGTGCCCGAGGTGCCGGTGCGGACGCGGCTGGCGGGGCT
GGAGCCATTCACGATGGCGGCCGCCTAG

Upstream 100 bases:

>100_bases
GCGATTTCCATTTCTACACGCTGAACCGCGCCGAACTCAGCTATGCCATTTGTCATTTGCTGGGGCTGCGGCCAGTATCG
ACATTGAAGGATCGGGCAGC

Downstream 100 bases:

>100_bases
TCCCCCTCCCGCTTGCGGGAGGGGTTAGGGGAGGGGCTGTCCCTGTCCGACGGCGCCGCCCGAGATTTGAAACAGACCCT
CCCCCGACCCCTCCCGCAAG

Product: 5-methyltetrahydrofolate--homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTSSLASSARDALKEAAKQRILIKDGPFGTAFQHRNAYVGVDGAEHGLSIDPRGNGDYLNLARPDIVADIMRDYLVAGAD
IIATNTFSANRISQADYGAEHLVAGINLASARLARREADAMQQKDGRRRFVAGAVGPTNKTLSLSPDVNNPGFREIDFDE
LKDVYLEQVVALAEGGADFILIETIFDTLNAKAGIAATLEAEAALGRELPLMISMTLTDLSGRNLSGHTVEAFWYAVRHA
KPMTIGLNCSFGAAQLRPHVKVLSDIADALVMVYPNAGLPNELGEYDELPETTAELVREWAEHGQVNILGGCCGSTPDHI
AAMAKAVEGLAPRQVPEVPVRTRLAGLEPFTMAAA

Sequences:

>Translated_355_residues
MTSSLASSARDALKEAAKQRILIKDGPFGTAFQHRNAYVGVDGAEHGLSIDPRGNGDYLNLARPDIVADIMRDYLVAGAD
IIATNTFSANRISQADYGAEHLVAGINLASARLARREADAMQQKDGRRRFVAGAVGPTNKTLSLSPDVNNPGFREIDFDE
LKDVYLEQVVALAEGGADFILIETIFDTLNAKAGIAATLEAEAALGRELPLMISMTLTDLSGRNLSGHTVEAFWYAVRHA
KPMTIGLNCSFGAAQLRPHVKVLSDIADALVMVYPNAGLPNELGEYDELPETTAELVREWAEHGQVNILGGCCGSTPDHI
AAMAKAVEGLAPRQVPEVPVRTRLAGLEPFTMAAA
>Mature_354_residues
TSSLASSARDALKEAAKQRILIKDGPFGTAFQHRNAYVGVDGAEHGLSIDPRGNGDYLNLARPDIVADIMRDYLVAGADI
IATNTFSANRISQADYGAEHLVAGINLASARLARREADAMQQKDGRRRFVAGAVGPTNKTLSLSPDVNNPGFREIDFDEL
KDVYLEQVVALAEGGADFILIETIFDTLNAKAGIAATLEAEAALGRELPLMISMTLTDLSGRNLSGHTVEAFWYAVRHAK
PMTIGLNCSFGAAQLRPHVKVLSDIADALVMVYPNAGLPNELGEYDELPETTAELVREWAEHGQVNILGGCCGSTPDHIA
AMAKAVEGLAPRQVPEVPVRTRLAGLEPFTMAAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=356, Percent_Identity=46.6292134831461, Blast_Score=322, Evalue=5e-88,
Organism=Escherichia coli, GI1790450, Length=349, Percent_Identity=52.4355300859599, Blast_Score=339, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17535405, Length=351, Percent_Identity=48.4330484330484, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24585077, Length=328, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37948; Mature: 37817

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSLASSARDALKEAAKQRILIKDGPFGTAFQHRNAYVGVDGAEHGLSIDPRGNGDYLN
CCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEE
LARPDIVADIMRDYLVAGADIIATNTFSANRISQADYGAEHLVAGINLASARLARREADA
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
MQQKDGRRRFVAGAVGPTNKTLSLSPDVNNPGFREIDFDELKDVYLEQVVALAEGGADFI
HHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LIETIFDTLNAKAGIAATLEAEAALGRELPLMISMTLTDLSGRNLSGHTVEAFWYAVRHA
EHHHHHHHHCCCCCEEEEEEHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCC
KPMTIGLNCSFGAAQLRPHVKVLSDIADALVMVYPNAGLPNELGEYDELPETTAELVREW
CCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHCCHHCCCHHHHHHHHHH
AEHGQVNILGGCCGSTPDHIAAMAKAVEGLAPRQVPEVPVRTRLAGLEPFTMAAA
HHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
TSSLASSARDALKEAAKQRILIKDGPFGTAFQHRNAYVGVDGAEHGLSIDPRGNGDYLN
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEE
LARPDIVADIMRDYLVAGADIIATNTFSANRISQADYGAEHLVAGINLASARLARREADA
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
MQQKDGRRRFVAGAVGPTNKTLSLSPDVNNPGFREIDFDELKDVYLEQVVALAEGGADFI
HHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LIETIFDTLNAKAGIAATLEAEAALGRELPLMISMTLTDLSGRNLSGHTVEAFWYAVRHA
EHHHHHHHHCCCCCEEEEEEHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCC
KPMTIGLNCSFGAAQLRPHVKVLSDIADALVMVYPNAGLPNELGEYDELPETTAELVREW
CCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHCCHHCCCHHHHHHHHHH
AEHGQVNILGGCCGSTPDHIAAMAKAVEGLAPRQVPEVPVRTRLAGLEPFTMAAA
HHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]