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The map label for this gene is phoB [H]

Identifier: 99082727

GI number: 99082727

Start: 3056120

End: 3057391

Strand: Direct

Name: phoB [H]

Synonym: TM1040_2887

Alternate gene names: 99082727

Gene position: 3056120-3057391 (Clockwise)

Preceding gene: 99082725

Following gene: 99082729

Centisome position: 95.48

GC content: 56.37

Gene sequence:

>1272_bases
GTGCTGCCAGAGCATTCAATTGAAGGTGAAGACGTGCCTGTAAGCGGGTCCATCCGACGAGTGTTGGTGGTGGACGATAG
CCGCTTGCAGCGTCGAATCCTTGTCGCTTCCCTCAAGAAGTGGGGCTTTGACGTCGTCGAAGCCGAGAGCGGCGAGGATG
CGATGGACATCTGCCGCGCCGATCCGCCTGATCTCATCCTGAGTGACTGGATGATGCCGGGGATGAACGGCATTGAATTT
TGCCGCGCCTTTCGCGCCGACTCGCATGAAACCTATAGCTACTTTATTCTGCTGACCTCAAAGAGCGAGAAAAACGAAGT
AGCAGAGGGGCTTGATGCCGGCGCGGATGATTTTCTGACCAAGCCTGTAAGCTCGGACGAGCTGCGCGCCCGTATCTCTG
CGGGGGAGCGGATCCTGCGTATGCAGCGCGAACTCTTTGAGAAAAACCGGATTGTGTCGGAAACGCTGGATGAGTTGCAG
ACCGTATATGATGCGATCGACAAGGACCTTATTCAGGCCCGCAAGATCCAGGAATCTCTGGTTCCGGAGCTGACCCGTGC
TTTCGGCGCGTCAGAGGTAAACCTGCTGCTGAAGCCCTGTGGACATATCGGTGGTGATCTGGTCGGAATGTTCTCGCCGG
GGATCAATCGCCTCGGCTTTTACTCCATCGATGTTTCGGGACATGGCATCACCTCCGCGATGATGACCGCACGGCTGGGG
GGCTATCTGTCCTCGACCTATTTCGACCAGAACGTCGGAATGGAAAAGAAATTCAACCGTTTCTACGCTCTGCGCCCCCC
GGAAGAGGTTGCGAGTGTCCTGAATGCCCGTTTGATTGCCGACACCGGGATCGAAGAGTATTTCACGATGGCCTACTGCA
TCGCGGACCTGCGCACGGGCGTCATCAAGATGGTTCAGGCCGGCCATCCTCACCCACTGCTTTTGCGCGCGGATGGCTCG
ATGGAGTTCATTGGCAAAGGCGGGGTGCCGGTCGGGCTCGTGCCCGATGTTGCCTATGAGCAAGAAGAATTTGTGATGGA
GAAGGGTGACAGGCTGCTTCTCTATTCAGATGGTTTCACAGAAGCGCATCTCAAAGACGGATCTATGCTCGACACCGAAG
GCCTGGTCGAGTTGGTCAACCGCTGTGACCCAAGGCGCAGCGGCAAGGAATTCCTCGACGACCTCTATTGGCACCTCACG
CAAGTGATGGGCGATGACAAAGGGCTGGAGGACGATATTTCTGCCACCCTGTTTGAGTTTCAAGGCCCTTAG

Upstream 100 bases:

>100_bases
AAAAATCGACTAAAAGTTTATTTTTCATCAGCTATTAAGTTGATCAGGTGTTTTCTAAGAAGGTCACAAGGGGACACGCC
GCGTTAATGAGGGATTGTCG

Downstream 100 bases:

>100_bases
GGGCAGTCAAACATACCGCGCCAGCAGCGCCGCAAAATGTCGATCCCCCGCGTTACCCAGAACCGAAACTGCGTCATTGA
CCGGCATCCACAATGCGCGG

Product: response regulator receiver modulated serine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MLPEHSIEGEDVPVSGSIRRVLVVDDSRLQRRILVASLKKWGFDVVEAESGEDAMDICRADPPDLILSDWMMPGMNGIEF
CRAFRADSHETYSYFILLTSKSEKNEVAEGLDAGADDFLTKPVSSDELRARISAGERILRMQRELFEKNRIVSETLDELQ
TVYDAIDKDLIQARKIQESLVPELTRAFGASEVNLLLKPCGHIGGDLVGMFSPGINRLGFYSIDVSGHGITSAMMTARLG
GYLSSTYFDQNVGMEKKFNRFYALRPPEEVASVLNARLIADTGIEEYFTMAYCIADLRTGVIKMVQAGHPHPLLLRADGS
MEFIGKGGVPVGLVPDVAYEQEEFVMEKGDRLLLYSDGFTEAHLKDGSMLDTEGLVELVNRCDPRRSGKEFLDDLYWHLT
QVMGDDKGLEDDISATLFEFQGP

Sequences:

>Translated_423_residues
MLPEHSIEGEDVPVSGSIRRVLVVDDSRLQRRILVASLKKWGFDVVEAESGEDAMDICRADPPDLILSDWMMPGMNGIEF
CRAFRADSHETYSYFILLTSKSEKNEVAEGLDAGADDFLTKPVSSDELRARISAGERILRMQRELFEKNRIVSETLDELQ
TVYDAIDKDLIQARKIQESLVPELTRAFGASEVNLLLKPCGHIGGDLVGMFSPGINRLGFYSIDVSGHGITSAMMTARLG
GYLSSTYFDQNVGMEKKFNRFYALRPPEEVASVLNARLIADTGIEEYFTMAYCIADLRTGVIKMVQAGHPHPLLLRADGS
MEFIGKGGVPVGLVPDVAYEQEEFVMEKGDRLLLYSDGFTEAHLKDGSMLDTEGLVELVNRCDPRRSGKEFLDDLYWHLT
QVMGDDKGLEDDISATLFEFQGP
>Mature_423_residues
MLPEHSIEGEDVPVSGSIRRVLVVDDSRLQRRILVASLKKWGFDVVEAESGEDAMDICRADPPDLILSDWMMPGMNGIEF
CRAFRADSHETYSYFILLTSKSEKNEVAEGLDAGADDFLTKPVSSDELRARISAGERILRMQRELFEKNRIVSETLDELQ
TVYDAIDKDLIQARKIQESLVPELTRAFGASEVNLLLKPCGHIGGDLVGMFSPGINRLGFYSIDVSGHGITSAMMTARLG
GYLSSTYFDQNVGMEKKFNRFYALRPPEEVASVLNARLIADTGIEEYFTMAYCIADLRTGVIKMVQAGHPHPLLLRADGS
MEFIGKGGVPVGLVPDVAYEQEEFVMEKGDRLLLYSDGFTEAHLKDGSMLDTEGLVELVNRCDPRRSGKEFLDDLYWHLT
QVMGDDKGLEDDISATLFEFQGP

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=41.3793103448276, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788394, Length=139, Percent_Identity=31.6546762589928, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788550, Length=139, Percent_Identity=32.3741007194245, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788191, Length=115, Percent_Identity=33.9130434782609, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=38.4615384615385, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=34.9593495934959, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787487, Length=219, Percent_Identity=25.5707762557078, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=29.6610169491525, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=33.0508474576271, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 47117; Mature: 47117

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPEHSIEGEDVPVSGSIRRVLVVDDSRLQRRILVASLKKWGFDVVEAESGEDAMDICRA
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCC
DPPDLILSDWMMPGMNGIEFCRAFRADSHETYSYFILLTSKSEKNEVAEGLDAGADDFLT
CCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHC
KPVSSDELRARISAGERILRMQRELFEKNRIVSETLDELQTVYDAIDKDLIQARKIQESL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPELTRAFGASEVNLLLKPCGHIGGDLVGMFSPGINRLGFYSIDVSGHGITSAMMTARLG
HHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHH
GYLSSTYFDQNVGMEKKFNRFYALRPPEEVASVLNARLIADTGIEEYFTMAYCIADLRTG
HHHHHHHHCCCCCCHHHHCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VIKMVQAGHPHPLLLRADGSMEFIGKGGVPVGLVPDVAYEQEEFVMEKGDRLLLYSDGFT
HHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCC
EAHLKDGSMLDTEGLVELVNRCDPRRSGKEFLDDLYWHLTQVMGDDKGLEDDISATLFEF
CHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
QGP
CCC
>Mature Secondary Structure
MLPEHSIEGEDVPVSGSIRRVLVVDDSRLQRRILVASLKKWGFDVVEAESGEDAMDICRA
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCC
DPPDLILSDWMMPGMNGIEFCRAFRADSHETYSYFILLTSKSEKNEVAEGLDAGADDFLT
CCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHC
KPVSSDELRARISAGERILRMQRELFEKNRIVSETLDELQTVYDAIDKDLIQARKIQESL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPELTRAFGASEVNLLLKPCGHIGGDLVGMFSPGINRLGFYSIDVSGHGITSAMMTARLG
HHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHH
GYLSSTYFDQNVGMEKKFNRFYALRPPEEVASVLNARLIADTGIEEYFTMAYCIADLRTG
HHHHHHHHCCCCCCHHHHCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VIKMVQAGHPHPLLLRADGSMEFIGKGGVPVGLVPDVAYEQEEFVMEKGDRLLLYSDGFT
HHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCC
EAHLKDGSMLDTEGLVELVNRCDPRRSGKEFLDDLYWHLTQVMGDDKGLEDDISATLFEF
CHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
QGP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]