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The map label for this gene is ilvA [H]

Identifier: 99082725

GI number: 99082725

Start: 3054365

End: 3055591

Strand: Direct

Name: ilvA [H]

Synonym: TM1040_2885

Alternate gene names: 99082725

Gene position: 3054365-3055591 (Clockwise)

Preceding gene: 99082724

Following gene: 99082727

Centisome position: 95.42

GC content: 58.76

Gene sequence:

>1227_bases
ATGACCAACTTCAGAACCCAGGCCAAAGCGGCCGAGGCGGCGATGCGCGATGTCTTCCCGCCCACGCCGCTGCAACGCAA
CGAACTCCTGTCGCAGCGCTTTGGTGCGGAGATTTACCTCAAACGCGAGGATCTCAGCCCGGTGCGCTCCTACAAGATTC
GCGGCGCGCTCAACGCGATGCGAAAGCAGACCAGCAAGGATCTGTTTGTCTGTGCTTCGGCGGGAAATCACGCGCAGGGC
ATGGCTTACATGTGTCGGCAAATGGACAAACGGGGTGTGATCTTTATGCCGGTGACCACACCTCAGCAGAAGATCCAGAA
GACCCGCATGTTTGGCGGTGACAACGTCGAGGTGCATCTGATCGGGGATTACTTCGACGATACATTGTCCGCCGCGCAGG
CGTGGTGCGCAGAAAAAGGAGGGTATTTCCTCTCTCCTTTTGACGACGCGGATGTCATCGAAGGTCAGGCATCAATCGCA
GTGGAAATCGAGGCGCAACTCGGCAAGGCGCCGGATCATATCGTTCTACCGGTGGGCGGCGGGGGTATGTCCTCTGGCGT
GGTTCGGTATTTTGGCGAGGATGTGCATGCGCTTCTTGTGGAGCCTGAGGGCGGCGCCTGCCTGAAGGCGGCGCTTGAGG
CAGGTCATCCCACACCGCTCAACCGGGTGGATACCTTTGTGGACGGCGCCGCCGTTGGCAAAATTGGCGAGCGGCCTTTT
GATATTTTGCGCAGCGTGCCGTTGCCGGATGTCCTGACTGTGTCAGAGGACCGGATCTGCACCACCATCCTCGAGATGCT
CAATGTCGAAGGTATCGTTCTGGAACCTGCAGGCGCGCTCGCCGTCGAGGCGCTGGGCGACCTGCGCACATGGATTAAGG
GTAAGACCGTGGTTTGTCTGACTTCCGGCGGGAACTTTGATTTCGAGCGACTGCCAGAGGTCAAGGAACGGGCGCAGCGC
TATTCCGGGGTGAAGAAATACTTCCTGCTGCGCCTGCCGCAACGTCCCGGCGCGCTCAAGGAATTCCTGAACATTCTTGG
GCCGGATGATGACATCGCACGTTTTGAATACATGAAGAAGTCTGCGCGCAACTTCGGCTCGGTCCTGATCGGGATCGAGA
CCAAGCGCCCAGAGAACTTCGCGCCGCTGTTTGCGCAACTGGATGCGGCGGGTTTCACCTACACCGACATCACAAATGAC
GAGACACTGGCGCAGTTCGTGATCTGA

Upstream 100 bases:

>100_bases
CCACTACGTCCAGGGTCGTTCTATCTGAAACGGCTCCAACATCATGCGTGAAACGCTGCGCCCTCTTGCCTTGGGCGTGG
CTTTGGGTCACGAAATCCAT

Downstream 100 bases:

>100_bases
GAAGATGAAGCAACACGTTGCGCCGCGTCGTCTATGCCAGTTCTCGGACGTTTTGGAGGAAGCTCGTCTTGGAAGCTGTA
TAGCTCGACATCACCCGGGT

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTNFRTQAKAAEAAMRDVFPPTPLQRNELLSQRFGAEIYLKREDLSPVRSYKIRGALNAMRKQTSKDLFVCASAGNHAQG
MAYMCRQMDKRGVIFMPVTTPQQKIQKTRMFGGDNVEVHLIGDYFDDTLSAAQAWCAEKGGYFLSPFDDADVIEGQASIA
VEIEAQLGKAPDHIVLPVGGGGMSSGVVRYFGEDVHALLVEPEGGACLKAALEAGHPTPLNRVDTFVDGAAVGKIGERPF
DILRSVPLPDVLTVSEDRICTTILEMLNVEGIVLEPAGALAVEALGDLRTWIKGKTVVCLTSGGNFDFERLPEVKERAQR
YSGVKKYFLLRLPQRPGALKEFLNILGPDDDIARFEYMKKSARNFGSVLIGIETKRPENFAPLFAQLDAAGFTYTDITND
ETLAQFVI

Sequences:

>Translated_408_residues
MTNFRTQAKAAEAAMRDVFPPTPLQRNELLSQRFGAEIYLKREDLSPVRSYKIRGALNAMRKQTSKDLFVCASAGNHAQG
MAYMCRQMDKRGVIFMPVTTPQQKIQKTRMFGGDNVEVHLIGDYFDDTLSAAQAWCAEKGGYFLSPFDDADVIEGQASIA
VEIEAQLGKAPDHIVLPVGGGGMSSGVVRYFGEDVHALLVEPEGGACLKAALEAGHPTPLNRVDTFVDGAAVGKIGERPF
DILRSVPLPDVLTVSEDRICTTILEMLNVEGIVLEPAGALAVEALGDLRTWIKGKTVVCLTSGGNFDFERLPEVKERAQR
YSGVKKYFLLRLPQRPGALKEFLNILGPDDDIARFEYMKKSARNFGSVLIGIETKRPENFAPLFAQLDAAGFTYTDITND
ETLAQFVI
>Mature_407_residues
TNFRTQAKAAEAAMRDVFPPTPLQRNELLSQRFGAEIYLKREDLSPVRSYKIRGALNAMRKQTSKDLFVCASAGNHAQGM
AYMCRQMDKRGVIFMPVTTPQQKIQKTRMFGGDNVEVHLIGDYFDDTLSAAQAWCAEKGGYFLSPFDDADVIEGQASIAV
EIEAQLGKAPDHIVLPVGGGGMSSGVVRYFGEDVHALLVEPEGGACLKAALEAGHPTPLNRVDTFVDGAAVGKIGERPFD
ILRSVPLPDVLTVSEDRICTTILEMLNVEGIVLEPAGALAVEALGDLRTWIKGKTVVCLTSGGNFDFERLPEVKERAQRY
SGVKKYFLLRLPQRPGALKEFLNILGPDDDIARFEYMKKSARNFGSVLIGIETKRPENFAPLFAQLDAAGFTYTDITNDE
TLAQFVI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=326, Percent_Identity=28.5276073619632, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI33469958, Length=314, Percent_Identity=29.2993630573248, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI19923959, Length=271, Percent_Identity=29.520295202952, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790207, Length=389, Percent_Identity=34.1902313624679, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789505, Length=301, Percent_Identity=31.2292358803987, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789235, Length=285, Percent_Identity=22.1052631578947, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71991565, Length=333, Percent_Identity=32.4324324324324, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17508781, Length=256, Percent_Identity=30.46875, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537387, Length=347, Percent_Identity=28.2420749279539, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320930, Length=412, Percent_Identity=32.7669902912621, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322631, Length=308, Percent_Identity=28.8961038961039, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319788, Length=297, Percent_Identity=24.5791245791246, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355833, Length=323, Percent_Identity=28.4829721362229, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 44774; Mature: 44643

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFRTQAKAAEAAMRDVFPPTPLQRNELLSQRFGAEIYLKREDLSPVRSYKIRGALNAM
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
RKQTSKDLFVCASAGNHAQGMAYMCRQMDKRGVIFMPVTTPQQKIQKTRMFGGDNVEVHL
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEE
IGDYFDDTLSAAQAWCAEKGGYFLSPFDDADVIEGQASIAVEIEAQLGKAPDHIVLPVGG
ECCHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCEEEEEEECCCCCCCCEEEEEECC
GGMSSGVVRYFGEDVHALLVEPEGGACLKAALEAGHPTPLNRVDTFVDGAAVGKIGERPF
CCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHCCCCCCHH
DILRSVPLPDVLTVSEDRICTTILEMLNVEGIVLEPAGALAVEALGDLRTWIKGKTVVCL
HHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEE
TSGGNFDFERLPEVKERAQRYSGVKKYFLLRLPQRPGALKEFLNILGPDDDIARFEYMKK
ECCCCCCHHHCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHH
SARNFGSVLIGIETKRPENFAPLFAQLDAAGFTYTDITNDETLAQFVI
HHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHCC
>Mature Secondary Structure 
TNFRTQAKAAEAAMRDVFPPTPLQRNELLSQRFGAEIYLKREDLSPVRSYKIRGALNAM
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
RKQTSKDLFVCASAGNHAQGMAYMCRQMDKRGVIFMPVTTPQQKIQKTRMFGGDNVEVHL
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEE
IGDYFDDTLSAAQAWCAEKGGYFLSPFDDADVIEGQASIAVEIEAQLGKAPDHIVLPVGG
ECCHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCEEEEEEECCCCCCCCEEEEEECC
GGMSSGVVRYFGEDVHALLVEPEGGACLKAALEAGHPTPLNRVDTFVDGAAVGKIGERPF
CCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHCCCCCCHH
DILRSVPLPDVLTVSEDRICTTILEMLNVEGIVLEPAGALAVEALGDLRTWIKGKTVVCL
HHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEE
TSGGNFDFERLPEVKERAQRYSGVKKYFLLRLPQRPGALKEFLNILGPDDDIARFEYMKK
ECCCCCCHHHCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHH
SARNFGSVLIGIETKRPENFAPLFAQLDAAGFTYTDITNDETLAQFVI
HHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]