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The map label for this gene is hslU

Identifier: 99082691

GI number: 99082691

Start: 3021606

End: 3022916

Strand: Direct

Name: hslU

Synonym: TM1040_2851

Alternate gene names: 99082691

Gene position: 3021606-3022916 (Clockwise)

Preceding gene: 99082690

Following gene: 99082692

Centisome position: 94.4

GC content: 57.28

Gene sequence:

>1311_bases
ATGACAGATCTGACCCCCCGCGAAATCGTTTCTGAACTCGACCGGTTCATCATTGGCCAAAAGGATGCCAAACGCGCTGT
CGCTGTAGCGTTGCGCAATCGCTGGCGCCGCAAACAGCTACCGGATGACCTGCGCGACGAAGTGCATCCAAAGAACATCC
TGATGATCGGCCCCACTGGCGTCGGCAAGACCGAAATTTCGCGCCGCTTGGCGAAGCTGGCGCGCGCGCCTTTCATCAAG
GTCGAAGCTACCAAATTCACCGAGGTTGGCTATGTCGGCCGCGACGTGGAACAAATCGTGCGTGATCTTGTGGATACCGC
AATCGTGCAAACCCGCGAGCACATGCGAGAGGACGTCAAAGCCAAGGCGCATAAAGCCGCCGAGGACCGTGTGCTCGAAG
CGATCGCCGGAACCGATGCCCGCGAGAGCACGCTCGAGATGTTCCGCAAAAAGCTCAAGGCAGGTGAGCTTGATGACACG
GTGATCGAGTTGGACATCGCCGATACCTCCAACCCCATGGGCGGTATGTTTGAAATTCCGGGTCAGCCAGGTGCAAACAT
GGGGATGATGAACCTCGGTGATCTCTTCGGAAAAGCCATGGGCGGGCGTACCACACGCAAAAAGCTCACCGTTGCAGAGA
GCTATGACGTGTTGATCGGGGAAGAAGCGGACAAGCTTCTGGATGATGAAACCGTAAACAAGGCTGCATTGGAAGCAGTA
GAGCAGAACGGGATCGTGTTCCTTGATGAGATCGACAAGGTCTGCGCCCGTTCCGATGCGCGTGGTGGCGACGTCAGCCG
TGAGGGCGTGCAGCGGGACTTGCTGCCGCTGATCGAAGGCACCACTGTCAGCACCAAACATGGCCCAGTCAAAACCGACC
ATATCCTGTTCATAGCGTCCGGTGCGTTCCACATCGCCAAGCCTTCTGATCTCCTGCCCGAGCTGCAAGGACGTTTGCCG
ATCCGCGTAAACCTGCGCGCCCTCAGTGAAGAGGATTTTGTGCGCATCCTGACCGAAACCGACAATGCGCTGACACGCCA
GTACGAGGCGCTCTTGGGCACAGAAAAAGTCAAAGTGACCTTCACCAAGGACGGGATCCACGCCCTTGCGCAGATTGCCG
CCGAAGTGAACCACACGGTGGAGAACATCGGCGCGCGGCGTCTCTACACGGTAATGGAGCGGGTCTTTGAGGAGATGTCC
TTTGCTGCGCCGGATCGATCCGGTGAAGAGATCATCGTAGATGAGCCCTTTGTGACCAAGAATTTGGGCGAATTGACCAA
ATCCACCGATCTCAGCCGCTACGTGCTCTGA

Upstream 100 bases:

>100_bases
CGCCTTCTGGGCCCGGATCCGCGCCGCGCTGCTTTCACTCCTTTCTGGTGTGCTGCCGCTTCATCGGCTGCCACCTTCTC
ACTGACCTCAAGGGATAGAC

Downstream 100 bases:

>100_bases
AATCGGAACGCGTATATCTATGCCTTGCCCGACAACACCGCCGGGCAAGGCTCATCCGAGGCGCAAAAAAGGGGCCAACA
TGAAGAACGCCATGCTGGTG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTDLTPREIVSELDRFIIGQKDAKRAVAVALRNRWRRKQLPDDLRDEVHPKNILMIGPTGVGKTEISRRLAKLARAPFIK
VEATKFTEVGYVGRDVEQIVRDLVDTAIVQTREHMREDVKAKAHKAAEDRVLEAIAGTDARESTLEMFRKKLKAGELDDT
VIELDIADTSNPMGGMFEIPGQPGANMGMMNLGDLFGKAMGGRTTRKKLTVAESYDVLIGEEADKLLDDETVNKAALEAV
EQNGIVFLDEIDKVCARSDARGGDVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHIAKPSDLLPELQGRLP
IRVNLRALSEEDFVRILTETDNALTRQYEALLGTEKVKVTFTKDGIHALAQIAAEVNHTVENIGARRLYTVMERVFEEMS
FAAPDRSGEEIIVDEPFVTKNLGELTKSTDLSRYVL

Sequences:

>Translated_436_residues
MTDLTPREIVSELDRFIIGQKDAKRAVAVALRNRWRRKQLPDDLRDEVHPKNILMIGPTGVGKTEISRRLAKLARAPFIK
VEATKFTEVGYVGRDVEQIVRDLVDTAIVQTREHMREDVKAKAHKAAEDRVLEAIAGTDARESTLEMFRKKLKAGELDDT
VIELDIADTSNPMGGMFEIPGQPGANMGMMNLGDLFGKAMGGRTTRKKLTVAESYDVLIGEEADKLLDDETVNKAALEAV
EQNGIVFLDEIDKVCARSDARGGDVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHIAKPSDLLPELQGRLP
IRVNLRALSEEDFVRILTETDNALTRQYEALLGTEKVKVTFTKDGIHALAQIAAEVNHTVENIGARRLYTVMERVFEEMS
FAAPDRSGEEIIVDEPFVTKNLGELTKSTDLSRYVL
>Mature_435_residues
TDLTPREIVSELDRFIIGQKDAKRAVAVALRNRWRRKQLPDDLRDEVHPKNILMIGPTGVGKTEISRRLAKLARAPFIKV
EATKFTEVGYVGRDVEQIVRDLVDTAIVQTREHMREDVKAKAHKAAEDRVLEAIAGTDARESTLEMFRKKLKAGELDDTV
IELDIADTSNPMGGMFEIPGQPGANMGMMNLGDLFGKAMGGRTTRKKLTVAESYDVLIGEEADKLLDDETVNKAALEAVE
QNGIVFLDEIDKVCARSDARGGDVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHIAKPSDLLPELQGRLPI
RVNLRALSEEDFVRILTETDNALTRQYEALLGTEKVKVTFTKDGIHALAQIAAEVNHTVENIGARRLYTVMERVFEEMSF
AAPDRSGEEIIVDEPFVTKNLGELTKSTDLSRYVL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=95, Percent_Identity=38.9473684210526, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=56.3758389261745, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1786642, Length=232, Percent_Identity=32.7586206896552, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648289, Length=260, Percent_Identity=33.4615384615385, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648291, Length=260, Percent_Identity=33.4615384615385, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_SILST (Q1GCN3)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614845.1
- ProteinModelPortal:   Q1GCN3
- SMR:   Q1GCN3
- STRING:   Q1GCN3
- GeneID:   4076385
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2851
- NMPDR:   fig|292414.1.peg.1754
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   Q1GCN3
- ProtClustDB:   PRK05201
- BioCyc:   SSP292414:TM1040_2851-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2

EC number: NA

Molecular weight: Translated: 48369; Mature: 48238

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 249-249 BINDING 314-314 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTPREIVSELDRFIIGQKDAKRAVAVALRNRWRRKQLPDDLRDEVHPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCC
VGKTEISRRLAKLARAPFIKVEATKFTEVGYVGRDVEQIVRDLVDTAIVQTREHMREDVK
CCHHHHHHHHHHHHHCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHKAAEDRVLEAIAGTDARESTLEMFRKKLKAGELDDTVIELDIADTSNPMGGMFEIP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEECC
GQPGANMGMMNLGDLFGKAMGGRTTRKKLTVAESYDVLIGEEADKLLDDETVNKAALEAV
CCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEECCCCEEECCCHHHHHCHHHHHHHHHHHH
EQNGIVFLDEIDKVCARSDARGGDVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIAS
HCCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEC
GAFHIAKPSDLLPELQGRLPIRVNLRALSEEDFVRILTETDNALTRQYEALLGTEKVKVT
CCEEECCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEE
FTKDGIHALAQIAAEVNHTVENIGARRLYTVMERVFEEMSFAAPDRSGEEIIVDEPFVTK
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHH
NLGELTKSTDLSRYVL
HHHHHHHHCCCHHHCC
>Mature Secondary Structure 
TDLTPREIVSELDRFIIGQKDAKRAVAVALRNRWRRKQLPDDLRDEVHPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEECCCC
VGKTEISRRLAKLARAPFIKVEATKFTEVGYVGRDVEQIVRDLVDTAIVQTREHMREDVK
CCHHHHHHHHHHHHHCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHKAAEDRVLEAIAGTDARESTLEMFRKKLKAGELDDTVIELDIADTSNPMGGMFEIP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEECC
GQPGANMGMMNLGDLFGKAMGGRTTRKKLTVAESYDVLIGEEADKLLDDETVNKAALEAV
CCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEECCCCEEECCCHHHHHCHHHHHHHHHHHH
EQNGIVFLDEIDKVCARSDARGGDVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIAS
HCCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEC
GAFHIAKPSDLLPELQGRLPIRVNLRALSEEDFVRILTETDNALTRQYEALLGTEKVKVT
CCEEECCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEE
FTKDGIHALAQIAAEVNHTVENIGARRLYTVMERVFEEMSFAAPDRSGEEIIVDEPFVTK
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHH
NLGELTKSTDLSRYVL
HHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA