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The map label for this gene is yafC [C]

Identifier: 99082300

GI number: 99082300

Start: 2602719

End: 2603624

Strand: Reverse

Name: yafC [C]

Synonym: TM1040_2460

Alternate gene names: 99082300

Gene position: 2603624-2602719 (Counterclockwise)

Preceding gene: 99082304

Following gene: 99082299

Centisome position: 81.34

GC content: 58.5

Gene sequence:

>906_bases
ATGGACCGACTGACCGAGATGGAAGCCTTCGCCAATGTGGTGGATCAGGGTGGCTTCACCGACGCCGCCAAGAAGATGGG
AATCTCCAAGTCGGCTGTGTCCAAACATGTGTCCAGTCTCGAAGCGCGCCTTGGCGCGCGCTTGTTGAACCGCACCACGC
GGCGGGTCTCTCCTACCGAGATCGGCCTTGCCTATTATGATCGCGCGCGCCGGGTGCTTAATGACGCAGGCGAGGCCGAT
GCATTGGTCACCTCGATGCAATCGGCGCCTTCCGGGCTTCTGCGGATCTCCGTAGCGACAGATTTTGGGGTCAACCATCT
CTCGCCGGTGCTGTCGGATTTCCTGCGCGATTTTCCCGATATTACGGTCAACATGGTGCTCAACAATCGCTTCGTGGAGC
TGATCTCCGAAGGTTTTGACATGGCGTTGCGCATCGGAGAGCTGGAAGACAGCTCACTTCGGGCGCGCAAGCTCACAGAG
ACCACCAAGCGGATGGTGGCCGCCCCCGAATACCTCGAGAAATATGGCCGACCGCAGAAGATTGATGATCTGAACGAGCA
CAAGCTCCTGCATTATTCCAACCAGTCCAGCGGCAACGTCTGGAAGCTGACCGCGCCCTCGGGCGAGAAGCGACAGGTGC
GCTCGACAGGTTGGCTTTCGGTGAACGATGGCCAGTCGCTCCTGAACGCAGCGATCTCTGGTTTGGGGATCGCCTATCTG
CCCAGCTATCTTTACGCTGAGGCCAAGGCCGAAGGACTGGTTGAGGACGTGATGCCCTCGCTGCCAGTCGAAACGCTCGG
GATCTATGCGGTTTACCCGCCGGGGCGTTTTACCCAACCCAAGGTGCGCGCATTCATTGACTTTCTCGTTCATGCCTTTG
CCGAAAAAGGCATGGAAACCTGGTAG

Upstream 100 bases:

>100_bases
ATCCGGCGAAAACCCCGCAATTATCCCAAGCGCGCCCCGTTCTTGCCCGATTTGCAGCGCAGGAAGAGCAAGATACAGAA
TGTGCGACGAGGAAAGCGAG

Downstream 100 bases:

>100_bases
GTCGCCACGTGCGATCTCCTTCCTCCCTGACCGCAAGGTCAATTCAAGACTCCCTCGAAAGACTTGCCCCTCGCCCGAAA
AGCGAGGGGCTTTTTTCTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDRLTEMEAFANVVDQGGFTDAAKKMGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTEIGLAYYDRARRVLNDAGEAD
ALVTSMQSAPSGLLRISVATDFGVNHLSPVLSDFLRDFPDITVNMVLNNRFVELISEGFDMALRIGELEDSSLRARKLTE
TTKRMVAAPEYLEKYGRPQKIDDLNEHKLLHYSNQSSGNVWKLTAPSGEKRQVRSTGWLSVNDGQSLLNAAISGLGIAYL
PSYLYAEAKAEGLVEDVMPSLPVETLGIYAVYPPGRFTQPKVRAFIDFLVHAFAEKGMETW

Sequences:

>Translated_301_residues
MDRLTEMEAFANVVDQGGFTDAAKKMGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTEIGLAYYDRARRVLNDAGEAD
ALVTSMQSAPSGLLRISVATDFGVNHLSPVLSDFLRDFPDITVNMVLNNRFVELISEGFDMALRIGELEDSSLRARKLTE
TTKRMVAAPEYLEKYGRPQKIDDLNEHKLLHYSNQSSGNVWKLTAPSGEKRQVRSTGWLSVNDGQSLLNAAISGLGIAYL
PSYLYAEAKAEGLVEDVMPSLPVETLGIYAVYPPGRFTQPKVRAFIDFLVHAFAEKGMETW
>Mature_301_residues
MDRLTEMEAFANVVDQGGFTDAAKKMGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTEIGLAYYDRARRVLNDAGEAD
ALVTSMQSAPSGLLRISVATDFGVNHLSPVLSDFLRDFPDITVNMVLNNRFVELISEGFDMALRIGELEDSSLRARKLTE
TTKRMVAAPEYLEKYGRPQKIDDLNEHKLLHYSNQSSGNVWKLTAPSGEKRQVRSTGWLSVNDGQSLLNAAISGLGIAYL
PSYLYAEAKAEGLVEDVMPSLPVETLGIYAVYPPGRFTQPKVRAFIDFLVHAFAEKGMETW

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=38.1118881118881, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=34.3434343434343, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=33.7837837837838, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=29.4736842105263, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=32.0819112627986, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=30.5084745762712, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=28.4722222222222, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=25.6849315068493, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=25.0847457627119, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790011, Length=247, Percent_Identity=25.5060728744939, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789173, Length=257, Percent_Identity=25.6809338521401, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790262, Length=297, Percent_Identity=25.5892255892256, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI157672245, Length=184, Percent_Identity=25, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=144, Percent_Identity=31.25, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=22.4080267558528, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33262; Mature: 33262

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLTEMEAFANVVDQGGFTDAAKKMGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTE
CCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IGLAYYDRARRVLNDAGEADALVTSMQSAPSGLLRISVATDFGVNHLSPVLSDFLRDFPD
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
ITVNMVLNNRFVELISEGFDMALRIGELEDSSLRARKLTETTKRMVAAPEYLEKYGRPQK
CEEEEHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
IDDLNEHKLLHYSNQSSGNVWKLTAPSGEKRQVRSTGWLSVNDGQSLLNAAISGLGIAYL
CCCCCCCCEEEECCCCCCCEEEEECCCCCCHHHHCCCCEEECCCHHHHHHHHHHCHHHHH
PSYLYAEAKAEGLVEDVMPSLPVETLGIYAVYPPGRFTQPKVRAFIDFLVHAFAEKGMET
HHHHHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
W
C
>Mature Secondary Structure
MDRLTEMEAFANVVDQGGFTDAAKKMGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTE
CCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IGLAYYDRARRVLNDAGEADALVTSMQSAPSGLLRISVATDFGVNHLSPVLSDFLRDFPD
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
ITVNMVLNNRFVELISEGFDMALRIGELEDSSLRARKLTETTKRMVAAPEYLEKYGRPQK
CEEEEHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
IDDLNEHKLLHYSNQSSGNVWKLTAPSGEKRQVRSTGWLSVNDGQSLLNAAISGLGIAYL
CCCCCCCCEEEECCCCCCCEEEEECCCCCCHHHHCCCCEEECCCHHHHHHHHHHCHHHHH
PSYLYAEAKAEGLVEDVMPSLPVETLGIYAVYPPGRFTQPKVRAFIDFLVHAFAEKGMET
HHHHHHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
W
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]