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The map label for this gene is oprF [H]

Identifier: 99082304

GI number: 99082304

Start: 2605380

End: 2606438

Strand: Reverse

Name: oprF [H]

Synonym: TM1040_2464

Alternate gene names: 99082304

Gene position: 2606438-2605380 (Counterclockwise)

Preceding gene: 99082305

Following gene: 99082300

Centisome position: 81.43

GC content: 62.98

Gene sequence:

>1059_bases
ATGGATCTGAAGGCTGCGCTTGTTGCGCTGTCTTTTGCGTCCATGGCGGTCGTGTTGTTCGGAGGTGCGGTGCAGGCGGC
CACGGTCGAGATCCCTCTGCCCGGCGCACCGGAGCTGTTGCGCACGCAAGAGACGCCGCTTGATACCTTGCTGTTGCCCA
TTGGTCCGGTGTCCGGCGATGATGTTCCCACCCGAGCCCTTGAGGGGCGGGTTCACCGGCGTAGTTGGCGGATCGACGCG
GACCTCAGCCCATTGGAGGTGCTTGCGCCAATCCGGGACGCGCTCACCGCGCGAGGATTTGAGGAAGTCTATGCCTGCAC
GGCGCAGGCCTGCGGCGGGTTTGGGTTTCGCTTTGGGATCGAGGTGATCCCTGCACCGGATATGGCAGTCAACCTTTCAA
ACTACGAGTTTCTCTCAGCGCAGGAGCCTCAATCCGGGACGGCGGTGTCGCTTCTGGTGTCCCGGCTGGGGGGCTCCATA
TACGTTCAAGTCATAGAGCGTCATGACCCGGCGCATCTTTTGCGCCCGCAAACGCTGCCCGCACTGGGCCCCAGCACGCA
GCCTGTGGACCGCGACACCATCCAAGCGGATGCCAGCGCACAGGGTGCCGCAAGCAATACCGAGAACGGATTGGAACAAG
CTCTGCTCGTTGATGCGATCAAAAAGGGTGGAAGCCTGAGCACCGGCGCCCTTCGGGATGTCTTGCAGTTGCAGGGCCAT
GTCCCGCTGCTGGATCTCGAATTCGATACGGCGTCGGCACAGGTGGGCGAGGGGCCTTATGATAGCCTCAAGCACCTTGC
GACCTTCATGAGCGATCATGAAAGCGCGGTCATCTTGCTCGTGGGTCACACCGATACGGTTGGGGGGCTGCAGGGCAATA
TCGCGCTTTCTGAACGCCGGGCCGCAAGCGTGCGCGAGCGTTTGATCACGCGCTACGGGGTCGACGGTGCGCGCATTCAG
GTGGCGGGGGCCGGATATATGGCTCCCTTGGCGCCCAACACTTCCGAGGAGGGGCGCGAAATCAACCGCCGCGTCGAGGC
GGTTCTGGTGCGCCCCTGA

Upstream 100 bases:

>100_bases
GCTTCTTCAACATGACAAACCGTGTCGCCAGCGCCACTGCCATGATGCCAAACGCAGAATACCACGGGCAGTTTCGGTGA
CCACGAAGGCTGGCTCTCTC

Downstream 100 bases:

>100_bases
GCACACATCATCCGAAAAGCATCCATATTGCGATCCCGACCAAGAGGCATGCAAAGGCGGTGTTGATCCCTCGCGCTTGC
ACAGAATGGCAAAAGACGCG

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MDLKAALVALSFASMAVVLFGGAVQAATVEIPLPGAPELLRTQETPLDTLLLPIGPVSGDDVPTRALEGRVHRRSWRIDA
DLSPLEVLAPIRDALTARGFEEVYACTAQACGGFGFRFGIEVIPAPDMAVNLSNYEFLSAQEPQSGTAVSLLVSRLGGSI
YVQVIERHDPAHLLRPQTLPALGPSTQPVDRDTIQADASAQGAASNTENGLEQALLVDAIKKGGSLSTGALRDVLQLQGH
VPLLDLEFDTASAQVGEGPYDSLKHLATFMSDHESAVILLVGHTDTVGGLQGNIALSERRAASVRERLITRYGVDGARIQ
VAGAGYMAPLAPNTSEEGREINRRVEAVLVRP

Sequences:

>Translated_352_residues
MDLKAALVALSFASMAVVLFGGAVQAATVEIPLPGAPELLRTQETPLDTLLLPIGPVSGDDVPTRALEGRVHRRSWRIDA
DLSPLEVLAPIRDALTARGFEEVYACTAQACGGFGFRFGIEVIPAPDMAVNLSNYEFLSAQEPQSGTAVSLLVSRLGGSI
YVQVIERHDPAHLLRPQTLPALGPSTQPVDRDTIQADASAQGAASNTENGLEQALLVDAIKKGGSLSTGALRDVLQLQGH
VPLLDLEFDTASAQVGEGPYDSLKHLATFMSDHESAVILLVGHTDTVGGLQGNIALSERRAASVRERLITRYGVDGARIQ
VAGAGYMAPLAPNTSEEGREINRRVEAVLVRP
>Mature_352_residues
MDLKAALVALSFASMAVVLFGGAVQAATVEIPLPGAPELLRTQETPLDTLLLPIGPVSGDDVPTRALEGRVHRRSWRIDA
DLSPLEVLAPIRDALTARGFEEVYACTAQACGGFGFRFGIEVIPAPDMAVNLSNYEFLSAQEPQSGTAVSLLVSRLGGSI
YVQVIERHDPAHLLRPQTLPALGPSTQPVDRDTIQADASAQGAASNTENGLEQALLVDAIKKGGSLSTGALRDVLQLQGH
VPLLDLEFDTASAQVGEGPYDSLKHLATFMSDHESAVILLVGHTDTVGGLQGNIALSERRAASVRERLITRYGVDGARIQ
VAGAGYMAPLAPNTSEEGREINRRVEAVLVRP

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=105, Percent_Identity=37.1428571428571, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 37247; Mature: 37247

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKAALVALSFASMAVVLFGGAVQAATVEIPLPGAPELLRTQETPLDTLLLPIGPVSGD
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCC
DVPTRALEGRVHRRSWRIDADLSPLEVLAPIRDALTARGFEEVYACTAQACGGFGFRFGI
CCCHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECE
EVIPAPDMAVNLSNYEFLSAQEPQSGTAVSLLVSRLGGSIYVQVIERHDPAHLLRPQTLP
EEECCCCEEEECCCCEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHCCCCCCC
ALGPSTQPVDRDTIQADASAQGAASNTENGLEQALLVDAIKKGGSLSTGALRDVLQLQGH
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
VPLLDLEFDTASAQVGEGPYDSLKHLATFMSDHESAVILLVGHTDTVGGLQGNIALSERR
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEHHHH
AASVRERLITRYGVDGARIQVAGAGYMAPLAPNTSEEGREINRRVEAVLVRP
HHHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCCHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MDLKAALVALSFASMAVVLFGGAVQAATVEIPLPGAPELLRTQETPLDTLLLPIGPVSGD
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCC
DVPTRALEGRVHRRSWRIDADLSPLEVLAPIRDALTARGFEEVYACTAQACGGFGFRFGI
CCCHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECE
EVIPAPDMAVNLSNYEFLSAQEPQSGTAVSLLVSRLGGSIYVQVIERHDPAHLLRPQTLP
EEECCCCEEEECCCCEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHCCCCCCC
ALGPSTQPVDRDTIQADASAQGAASNTENGLEQALLVDAIKKGGSLSTGALRDVLQLQGH
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
VPLLDLEFDTASAQVGEGPYDSLKHLATFMSDHESAVILLVGHTDTVGGLQGNIALSERR
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEHHHH
AASVRERLITRYGVDGARIQVAGAGYMAPLAPNTSEEGREINRRVEAVLVRP
HHHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCCHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]