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The map label for this gene is 99082299

Identifier: 99082299

GI number: 99082299

Start: 2601478

End: 2602539

Strand: Reverse

Name: 99082299

Synonym: TM1040_2459

Alternate gene names: NA

Gene position: 2602539-2601478 (Counterclockwise)

Preceding gene: 99082300

Following gene: 99082297

Centisome position: 81.31

GC content: 62.71

Gene sequence:

>1062_bases
ATGACCAAGGGAGAGGCTTTTCTGGATCGTCTTGGGATTTCGCTGCCTCTGATACAGGCGCCGATGGCCGGGGTCTCAAC
TCCGAGGCTCGCAGCAGCAGTGACGGACGCCGGCGCGCTTGGTTCGATCGGGGTGGGGGCCCTGGATGCTAGCGCGACTC
GCAAGCAGATCGAAGAGCTGCAGCATCTGACCAATGGGCCCTTCAATGTGAATTTCTTTTGCCATCAGACGCCGCAGCGC
AACCCTGAGATCGAGGGTCGCTGGATCCAACGCAGCGCGCCTTTGTTTGCCCGCATGCAGGCGGAGCCACCCAAAGAGCT
ATCTGAAATATATACCAGTTTTCGGGATACAGACGCATTTCTCGAGGCGGTGCTGGACCTGCGGCCTGCGGTGGTGAGTT
TCCACTTTGGTTTGCCCAAGCCCCATCAGATCAGCGCCATGAAGGCCGCAGGGCTGACACTGCTGGCGAGCGCCACCTCG
CTGGAGGAGGCCCGCCAGATCGAAGCGGCGGGGCTGCATGGGATCATAGCACAAGGGTGGGAGGCCGGCGGCCACCGGGG
TGTTTTTGAACCTGGCGCCTTGGATGCACGCCTTGGCCTGCTGGAGCTGGTTGCGGTGCTGGCCGAACAGACCACCCTGC
CGGTGATCGCAGCCGGTGGCCTGATGACGGGCGCGGAGATCGCGCGGGCGCTCTCGGCGGGGGCAGTCGCGGCACAGCTC
GGCACCGCGTTTATCGGCTGCCCGGAGAGCAACGCCGATGCTGCCTATCGGGACAGGCTGGCGCTTGGAGATGAGACGGT
GATGACCGCAGCGATCTCCGGTCGTCCGGCGCGATGTCTCAGCAATGCCTTCACCGAATGGGCCTCAGATGTCCCGATTG
CAGAAATTCCAGCCTACCCATGCGCTTATGATCTTGGCAAAGCCCTGAATGCAGTGGCAACGGCCAAGGGCGTCAGCAGT
TTTGGCTCGCAGTGGGCAGGCATGGGCGCAGATCGCGCTCGGTTTATGCCCGCGCAGGCCCTGGTGCAGACCTTGATGGC
TGAACTTGCCGCTACAATCTGA

Upstream 100 bases:

>100_bases
AAGCGAGGGGCTTTTTTCTCCGACCCTGTCGGAGTGGCCTTCCCGGCTGCATTGAGCCATTGTGATGGCAATCGAGATCA
CACGGCGGAGGGATGTTGCT

Downstream 100 bases:

>100_bases
AATGTGGCGTGTTCTCCGTCGGGCGAGGAGGACCCTCTCGTTCGGCTGCGCCAATGAACGAGAGGGCTCTGTTCAGAGCG
TGGCCGCGAGGCGGGTGCCC

Product: 2-nitropropane dioxygenase, NPD

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTKGEAFLDRLGISLPLIQAPMAGVSTPRLAAAVTDAGALGSIGVGALDASATRKQIEELQHLTNGPFNVNFFCHQTPQR
NPEIEGRWIQRSAPLFARMQAEPPKELSEIYTSFRDTDAFLEAVLDLRPAVVSFHFGLPKPHQISAMKAAGLTLLASATS
LEEARQIEAAGLHGIIAQGWEAGGHRGVFEPGALDARLGLLELVAVLAEQTTLPVIAAGGLMTGAEIARALSAGAVAAQL
GTAFIGCPESNADAAYRDRLALGDETVMTAAISGRPARCLSNAFTEWASDVPIAEIPAYPCAYDLGKALNAVATAKGVSS
FGSQWAGMGADRARFMPAQALVQTLMAELAATI

Sequences:

>Translated_353_residues
MTKGEAFLDRLGISLPLIQAPMAGVSTPRLAAAVTDAGALGSIGVGALDASATRKQIEELQHLTNGPFNVNFFCHQTPQR
NPEIEGRWIQRSAPLFARMQAEPPKELSEIYTSFRDTDAFLEAVLDLRPAVVSFHFGLPKPHQISAMKAAGLTLLASATS
LEEARQIEAAGLHGIIAQGWEAGGHRGVFEPGALDARLGLLELVAVLAEQTTLPVIAAGGLMTGAEIARALSAGAVAAQL
GTAFIGCPESNADAAYRDRLALGDETVMTAAISGRPARCLSNAFTEWASDVPIAEIPAYPCAYDLGKALNAVATAKGVSS
FGSQWAGMGADRARFMPAQALVQTLMAELAATI
>Mature_352_residues
TKGEAFLDRLGISLPLIQAPMAGVSTPRLAAAVTDAGALGSIGVGALDASATRKQIEELQHLTNGPFNVNFFCHQTPQRN
PEIEGRWIQRSAPLFARMQAEPPKELSEIYTSFRDTDAFLEAVLDLRPAVVSFHFGLPKPHQISAMKAAGLTLLASATSL
EEARQIEAAGLHGIIAQGWEAGGHRGVFEPGALDARLGLLELVAVLAEQTTLPVIAAGGLMTGAEIARALSAGAVAAQLG
TAFIGCPESNADAAYRDRLALGDETVMTAAISGRPARCLSNAFTEWASDVPIAEIPAYPCAYDLGKALNAVATAKGVSSF
GSQWAGMGADRARFMPAQALVQTLMAELAATI

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=338, Percent_Identity=34.3195266272189, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 36902; Mature: 36770

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKGEAFLDRLGISLPLIQAPMAGVSTPRLAAAVTDAGALGSIGVGALDASATRKQIEEL
CCCHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
QHLTNGPFNVNFFCHQTPQRNPEIEGRWIQRSAPLFARMQAEPPKELSEIYTSFRDTDAF
HHHCCCCEEEEEEECCCCCCCCCCCCEEEECCCCHHEECCCCCHHHHHHHHHHHCCHHHH
LEAVLDLRPAVVSFHFGLPKPHQISAMKAAGLTLLASATSLEEARQIEAAGLHGIIAQGW
HHHHHHHCHHHHEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
EAGGHRGVFEPGALDARLGLLELVAVLAEQTTLPVIAAGGLMTGAEIARALSAGAVAAQL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
GTAFIGCPESNADAAYRDRLALGDETVMTAAISGRPARCLSNAFTEWASDVPIAEIPAYP
HHHEEECCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHCCCCC
CAYDLGKALNAVATAKGVSSFGSQWAGMGADRARFMPAQALVQTLMAELAATI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKGEAFLDRLGISLPLIQAPMAGVSTPRLAAAVTDAGALGSIGVGALDASATRKQIEEL
CCHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
QHLTNGPFNVNFFCHQTPQRNPEIEGRWIQRSAPLFARMQAEPPKELSEIYTSFRDTDAF
HHHCCCCEEEEEEECCCCCCCCCCCCEEEECCCCHHEECCCCCHHHHHHHHHHHCCHHHH
LEAVLDLRPAVVSFHFGLPKPHQISAMKAAGLTLLASATSLEEARQIEAAGLHGIIAQGW
HHHHHHHCHHHHEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
EAGGHRGVFEPGALDARLGLLELVAVLAEQTTLPVIAAGGLMTGAEIARALSAGAVAAQL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
GTAFIGCPESNADAAYRDRLALGDETVMTAAISGRPARCLSNAFTEWASDVPIAEIPAYP
HHHEEECCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHCCCCC
CAYDLGKALNAVATAKGVSSFGSQWAGMGADRARFMPAQALVQTLMAELAATI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA