Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 99082218

GI number: 99082218

Start: 2497575

End: 2499782

Strand: Reverse

Name: priA [H]

Synonym: TM1040_2378

Alternate gene names: 99082218

Gene position: 2499782-2497575 (Counterclockwise)

Preceding gene: 99082224

Following gene: 99082217

Centisome position: 78.1

GC content: 63.18

Gene sequence:

>2208_bases
ATGAGTAAAGCAGGCGATAGCCCGGAGTTTTTTGCCCAAGGCGAGCGGGTCGGGGTGCTGACAGCGCAGCCGCTGGATCG
TCTGTTGGACTATCGCGCGCCCGAGGGCGGCTGCTTTCTCGGAGCCTTTGTCGAGGTGCCGCTTGGGCCGCGCAAGGTGA
TCGGCGTGGTCTGGGGTCCGGGCGCCGGTGACTTTGATGCGGCAAAGCTGCGCTCGGTGATCCGTGTACTGGATCTCGCA
CCGATGCGCGCCGAGATGCGCGGCTTTCTGGAGCGTGCAGGTCAGTATACGCTCACACCGATGTCGATGATGCTCCGGCT
GGCAACGCGCGCACCGGGTCTGGCGGACCCGCCTTCGATGCGCAAGATCCTGCGGCGCGGTGATCGTGAACCTGACCGCA
TCACAGACGCCCGCGCCAAGGTGCTTGATGTGATGCAGGAATATGAGGGCGGCGCGTTTACCCCGTCAGAGCTTTCTGAG
CTGGCTGGCGTGACCACTTCCGTCATCAAGGGGCTGATAAAACAGGGGGCGATCCGCGAAGAAGAAGCGCCCCGGGACCT
GCCATTTCCCCCTCTTGACCCAGATCTGCCGGGCAAGACGCTCTCTGAAGATCAGGCCGCAGCCGCCGCCGCCCTTGAGG
CCGGTGTGGAATCTGGGCGCTACGGTACGACCCTTCTCAAGGGGGTGACAGGGTCGGGCAAGACCGAAGTCTACCTTGAG
GCTGTGGCGGCCTGTCTGCGCAAGGGGCGGCAAGCCTTGGTGCTTTTGCCGGAGATTGCTCTCACTGCAGAGTTCCTGAA
ACGCGTGGAGGCGCGGTTTGGGGCGGTTCCGGCCGAATGGCACTCTGGCGTGACAATGACCGAACGCCGCCGCGTCTGGC
GCATGGTGGGGCAGGGGGCGGCGCAACTGGTGATTGGTGCGCGTTCCTCTCTCTATCTGCCGTTCCGCGATCTGGGGCTG
ATCATCGTCGATGAAGAGCACGACACGTCTTATAAACAAGAAGAAGGGGTGCTGTATAACGCGCGGGATCTGGCGGTCTG
GCGCGCGTCCTTGTGTCAGGCGCAGGTGGTTCTTGCGTCGGCCACCCCGAGCCTTGAAACCTGGGCAAACGCGGAGGCCG
GGAAATACCAGCGCCTTGATCTGACATCGCGCTTTGGCGACTCGGTGCTGCCGGAGATGCGCGCGATTGATATGCGCAAT
GAGGAGCTATTGCCCTCCACGTGGATTTCGCCGACGCTCAAACAGGCCATGAAACTGCGCCTCGAACGGGGCGAACAGTC
GCTTTTGTTCCTCAATCGGCGCGGCTTTGCTCCGGTGACGATCTGTCGGGCCTGTGGTGCGCAGGTGGCCTGCGACAATT
GCGATGCGCGAATGGTCGAGCATCGCTTCATGAAACGCCTGATGTGCCACCAATGTGGTGAAACCAAACCCGTGCCAACC
GAATGCCCCTCCTGCAAGGTCGAGGGCAAAATGGCCCCCGTTGGCCCCGGTATCGAACGTCTGGGCGAAGAAGCGGCAGC
CCTCTTTCCCGAGGCGCGCATCGCGGTGCTGAGCTCTGATCTCTTTGGATCTGCACGCGCGCTCAAACAGCGGATCGAGG
ATATTGCCGCAGGTGAGGCCGACATCATTCTGGGTACGCAATTGGTCGCGAAGGGACACAATTTCCCCCTGCTCACGCTT
GTGGGGGTGATCGACGCTGATCTCGGCTTGCAAGGCTCTGACCTGCGTGCGGCGGAGCGGACCTTTCAGCTCATGCGTCA
GGTGGCGGGGCGCGCTGGGCGCGCTGAACGCAGGGGCGAGGCCCTGATGCAGAGCTTTCAGCCCGAACATCCTGTGATCC
GCGCGATCCTATCGGGCGATGAGGAGAGCTTCTGGCGCGCAGAGGCAGAACAGCGACAGGCAGCGGGGGTGCCCCCCTAT
GGACGTATGGCGGGCGTGATCCTGTCGGGGACCGATCTAGCGCAGGTCTTTGATCTTGGCGGGGCCTTGGCACGCAACGA
TGGCCCCTTGCGGCAGGTGGGCGCGCAGCTTTTTGGTCCGGCGCCCGCCCCGATTGCGCGCGTCAGGGGGCGGCACCGTG
TGCGGCTCCTGGTGAAAGCCGACAAAGGCGCGCCCCTTCAAGATGCGATCACCCGCTGGATCGCGCCATTCAAGCTCAAG
GGCGATCTGCGCCTCAATGTGGACATTGATCCACAGAGCTTCCTTTAG

Upstream 100 bases:

>100_bases
CCATGGGGCAAAGCAGGGCGCGTCCGCGCGCATGTTAGCGTAAACAGCGGCGCGCGCCTACCGCCAGGTGCAGGAAATAC
AGAGCAGGGTCACACGCGGG

Downstream 100 bases:

>100_bases
GCTGTCACACAGTCGTAGCGCGCAGAAATGCAGAGGCGTCTGTGCGTGGCTTGGTACAATTCTTTTCTCGCCCACGCACA
AAAAGCGCACTAAAGCCAAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 735; Mature: 734

Protein sequence:

>735_residues
MSKAGDSPEFFAQGERVGVLTAQPLDRLLDYRAPEGGCFLGAFVEVPLGPRKVIGVVWGPGAGDFDAAKLRSVIRVLDLA
PMRAEMRGFLERAGQYTLTPMSMMLRLATRAPGLADPPSMRKILRRGDREPDRITDARAKVLDVMQEYEGGAFTPSELSE
LAGVTTSVIKGLIKQGAIREEEAPRDLPFPPLDPDLPGKTLSEDQAAAAAALEAGVESGRYGTTLLKGVTGSGKTEVYLE
AVAACLRKGRQALVLLPEIALTAEFLKRVEARFGAVPAEWHSGVTMTERRRVWRMVGQGAAQLVIGARSSLYLPFRDLGL
IIVDEEHDTSYKQEEGVLYNARDLAVWRASLCQAQVVLASATPSLETWANAEAGKYQRLDLTSRFGDSVLPEMRAIDMRN
EELLPSTWISPTLKQAMKLRLERGEQSLLFLNRRGFAPVTICRACGAQVACDNCDARMVEHRFMKRLMCHQCGETKPVPT
ECPSCKVEGKMAPVGPGIERLGEEAAALFPEARIAVLSSDLFGSARALKQRIEDIAAGEADIILGTQLVAKGHNFPLLTL
VGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAERRGEALMQSFQPEHPVIRAILSGDEESFWRAEAEQRQAAGVPPY
GRMAGVILSGTDLAQVFDLGGALARNDGPLRQVGAQLFGPAPAPIARVRGRHRVRLLVKADKGAPLQDAITRWIAPFKLK
GDLRLNVDIDPQSFL

Sequences:

>Translated_735_residues
MSKAGDSPEFFAQGERVGVLTAQPLDRLLDYRAPEGGCFLGAFVEVPLGPRKVIGVVWGPGAGDFDAAKLRSVIRVLDLA
PMRAEMRGFLERAGQYTLTPMSMMLRLATRAPGLADPPSMRKILRRGDREPDRITDARAKVLDVMQEYEGGAFTPSELSE
LAGVTTSVIKGLIKQGAIREEEAPRDLPFPPLDPDLPGKTLSEDQAAAAAALEAGVESGRYGTTLLKGVTGSGKTEVYLE
AVAACLRKGRQALVLLPEIALTAEFLKRVEARFGAVPAEWHSGVTMTERRRVWRMVGQGAAQLVIGARSSLYLPFRDLGL
IIVDEEHDTSYKQEEGVLYNARDLAVWRASLCQAQVVLASATPSLETWANAEAGKYQRLDLTSRFGDSVLPEMRAIDMRN
EELLPSTWISPTLKQAMKLRLERGEQSLLFLNRRGFAPVTICRACGAQVACDNCDARMVEHRFMKRLMCHQCGETKPVPT
ECPSCKVEGKMAPVGPGIERLGEEAAALFPEARIAVLSSDLFGSARALKQRIEDIAAGEADIILGTQLVAKGHNFPLLTL
VGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAERRGEALMQSFQPEHPVIRAILSGDEESFWRAEAEQRQAAGVPPY
GRMAGVILSGTDLAQVFDLGGALARNDGPLRQVGAQLFGPAPAPIARVRGRHRVRLLVKADKGAPLQDAITRWIAPFKLK
GDLRLNVDIDPQSFL
>Mature_734_residues
SKAGDSPEFFAQGERVGVLTAQPLDRLLDYRAPEGGCFLGAFVEVPLGPRKVIGVVWGPGAGDFDAAKLRSVIRVLDLAP
MRAEMRGFLERAGQYTLTPMSMMLRLATRAPGLADPPSMRKILRRGDREPDRITDARAKVLDVMQEYEGGAFTPSELSEL
AGVTTSVIKGLIKQGAIREEEAPRDLPFPPLDPDLPGKTLSEDQAAAAAALEAGVESGRYGTTLLKGVTGSGKTEVYLEA
VAACLRKGRQALVLLPEIALTAEFLKRVEARFGAVPAEWHSGVTMTERRRVWRMVGQGAAQLVIGARSSLYLPFRDLGLI
IVDEEHDTSYKQEEGVLYNARDLAVWRASLCQAQVVLASATPSLETWANAEAGKYQRLDLTSRFGDSVLPEMRAIDMRNE
ELLPSTWISPTLKQAMKLRLERGEQSLLFLNRRGFAPVTICRACGAQVACDNCDARMVEHRFMKRLMCHQCGETKPVPTE
CPSCKVEGKMAPVGPGIERLGEEAAALFPEARIAVLSSDLFGSARALKQRIEDIAAGEADIILGTQLVAKGHNFPLLTLV
GVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAERRGEALMQSFQPEHPVIRAILSGDEESFWRAEAEQRQAAGVPPYG
RMAGVILSGTDLAQVFDLGGALARNDGPLRQVGAQLFGPAPAPIARVRGRHRVRLLVKADKGAPLQDAITRWIAPFKLKG
DLRLNVDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=538, Percent_Identity=39.0334572490706, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 79963; Mature: 79832

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAGDSPEFFAQGERVGVLTAQPLDRLLDYRAPEGGCFLGAFVEVPLGPRKVIGVVWGP
CCCCCCCCHHHHCCCEEEEEEHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEECC
GAGDFDAAKLRSVIRVLDLAPMRAEMRGFLERAGQYTLTPMSMMLRLATRAPGLADPPSM
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCHHH
RKILRRGDREPDRITDARAKVLDVMQEYEGGAFTPSELSELAGVTTSVIKGLIKQGAIRE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEAPRDLPFPPLDPDLPGKTLSEDQAAAAAALEAGVESGRYGTTLLKGVTGSGKTEVYLE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHH
AVAACLRKGRQALVLLPEIALTAEFLKRVEARFGAVPAEWHSGVTMTERRRVWRMVGQGA
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCH
AQLVIGARSSLYLPFRDLGLIIVDEEHDTSYKQEEGVLYNARDLAVWRASLCQAQVVLAS
HEEEEECCCCEECCHHHCCEEEEECCCCCCCHHHCCCEECHHHHHHHHHHHHHHHHEEEC
ATPSLETWANAEAGKYQRLDLTSRFGDSVLPEMRAIDMRNEELLPSTWISPTLKQAMKLR
CCCCHHHHCCCCCCCEEEEEHHHHCCCHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
LERGEQSLLFLNRRGFAPVTICRACGAQVACDNCDARMVEHRFMKRLMCHQCGETKPVPT
HHCCCCEEEEEECCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
ECPSCKVEGKMAPVGPGIERLGEEAAALFPEARIAVLSSDLFGSARALKQRIEDIAAGEA
CCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIILGTQLVAKGHNFPLLTLVGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAERRGE
CEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
ALMQSFQPEHPVIRAILSGDEESFWRAEAEQRQAAGVPPYGRMAGVILSGTDLAQVFDLG
HHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHCEEECCCHHHHHHHCC
GALARNDGPLRQVGAQLFGPAPAPIARVRGRHRVRLLVKADKGAPLQDAITRWIAPFKLK
CHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCEEEC
GDLRLNVDIDPQSFL
CCEEEEEECCHHHCC
>Mature Secondary Structure 
SKAGDSPEFFAQGERVGVLTAQPLDRLLDYRAPEGGCFLGAFVEVPLGPRKVIGVVWGP
CCCCCCCHHHHCCCEEEEEEHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEECC
GAGDFDAAKLRSVIRVLDLAPMRAEMRGFLERAGQYTLTPMSMMLRLATRAPGLADPPSM
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCHHH
RKILRRGDREPDRITDARAKVLDVMQEYEGGAFTPSELSELAGVTTSVIKGLIKQGAIRE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEAPRDLPFPPLDPDLPGKTLSEDQAAAAAALEAGVESGRYGTTLLKGVTGSGKTEVYLE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHH
AVAACLRKGRQALVLLPEIALTAEFLKRVEARFGAVPAEWHSGVTMTERRRVWRMVGQGA
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCH
AQLVIGARSSLYLPFRDLGLIIVDEEHDTSYKQEEGVLYNARDLAVWRASLCQAQVVLAS
HEEEEECCCCEECCHHHCCEEEEECCCCCCCHHHCCCEECHHHHHHHHHHHHHHHHEEEC
ATPSLETWANAEAGKYQRLDLTSRFGDSVLPEMRAIDMRNEELLPSTWISPTLKQAMKLR
CCCCHHHHCCCCCCCEEEEEHHHHCCCHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
LERGEQSLLFLNRRGFAPVTICRACGAQVACDNCDARMVEHRFMKRLMCHQCGETKPVPT
HHCCCCEEEEEECCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
ECPSCKVEGKMAPVGPGIERLGEEAAALFPEARIAVLSSDLFGSARALKQRIEDIAAGEA
CCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIILGTQLVAKGHNFPLLTLVGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRAERRGE
CEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
ALMQSFQPEHPVIRAILSGDEESFWRAEAEQRQAAGVPPYGRMAGVILSGTDLAQVFDLG
HHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHCEEECCCHHHHHHHCC
GALARNDGPLRQVGAQLFGPAPAPIARVRGRHRVRLLVKADKGAPLQDAITRWIAPFKLK
CHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCEEEC
GDLRLNVDIDPQSFL
CCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]