Click here to switch to the map view.

The map label for this gene is truA

Identifier: 99082224

GI number: 99082224

Start: 2503881

End: 2504660

Strand: Reverse

Name: truA

Synonym: TM1040_2384

Alternate gene names: 99082224

Gene position: 2504660-2503881 (Counterclockwise)

Preceding gene: 99082228

Following gene: 99082218

Centisome position: 78.25

GC content: 60.51

Gene sequence:

>780_bases
ATGCCGCGTTTTGCTCTCAAAGTTGAATATCATGGAAAGCCGTTTGCCGGTTGGCAACGGCAGAAGGACCAACCGTCTGT
TCAGGGGGCGATTGAGGCAGCGCTCGCGCGGATCGAGCCGGGTGAGCACACGATTGCCGCCGCCGGACGCACCGATACGG
GGGTGCATGGGCTTGGGCAGGTTGCGCATTGCGACCTCGAGAAGAACTGGGATCCGTTTCGCCTGAGTGAAGCACTGAAC
TACCACCTGAAGCCCGCCCCGGTTGCGATCGTGGATGCAGCCCTTGTGGACGACGAATGGCATGCCCGCTTCAGTGCGGT
GGAGCGGCAATATCTGTTCCGCATCCTGATGCGCCGCGCTCCTGCGACTCACGATGAGGGGCAGGTCTGGCAGATCAAAC
ATGATCTGGATGTCGCGGCGATGCAGGCGGGAGCCAATATGCTGCTTGGAAATCACGATTTCACCACGTTTCGCTCGTCG
ATCTGTCAGGCTGCCAGCCCGGTGAAGACCTTGGATGAGCTGAGAGTGGAGCGCGTTCAGGGACTATCGGGTCCAGAGGT
GCATTTTCATGTGCGCGCGCGCAGCTTCCTACACAATCAGGTTCGCAGTTTTGTCGGAACACTTGAGCGTGTGGGCACCG
GAGCATGGAGCCCCGAGGACGTCAAACACGCGCTTGAGGCCAAGGACCGCGCGGCCTGTGGTCCTGTGTGCCCGGGCCAT
GGGCTCTATCTGGCGCGGGTGGGCTATCCTGATCCGGTTTTTTCTAGCGACCGGGTGTAA

Upstream 100 bases:

>100_bases
TTCACAGAACATAAGGGGGCGCACCTTTGGTTGCCAGTGCTGCTGTGGATTGCGTTCAGGAGGTCTTGCCAGAATTGCCG
TCGCGCGCTAGGCCGCGCCC

Downstream 100 bases:

>100_bases
GCTCGGCTGACGAGACCCAGCGCGTTTTGTGCAGATCTCATGACGGTTCGTGCGTGTTCTAAGTCAGGCGCGTGTGTTTG
AGCAGGGCGAAGTTGATGCT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPRFALKVEYHGKPFAGWQRQKDQPSVQGAIEAALARIEPGEHTIAAAGRTDTGVHGLGQVAHCDLEKNWDPFRLSEALN
YHLKPAPVAIVDAALVDDEWHARFSAVERQYLFRILMRRAPATHDEGQVWQIKHDLDVAAMQAGANMLLGNHDFTTFRSS
ICQAASPVKTLDELRVERVQGLSGPEVHFHVRARSFLHNQVRSFVGTLERVGTGAWSPEDVKHALEAKDRAACGPVCPGH
GLYLARVGYPDPVFSSDRV

Sequences:

>Translated_259_residues
MPRFALKVEYHGKPFAGWQRQKDQPSVQGAIEAALARIEPGEHTIAAAGRTDTGVHGLGQVAHCDLEKNWDPFRLSEALN
YHLKPAPVAIVDAALVDDEWHARFSAVERQYLFRILMRRAPATHDEGQVWQIKHDLDVAAMQAGANMLLGNHDFTTFRSS
ICQAASPVKTLDELRVERVQGLSGPEVHFHVRARSFLHNQVRSFVGTLERVGTGAWSPEDVKHALEAKDRAACGPVCPGH
GLYLARVGYPDPVFSSDRV
>Mature_258_residues
PRFALKVEYHGKPFAGWQRQKDQPSVQGAIEAALARIEPGEHTIAAAGRTDTGVHGLGQVAHCDLEKNWDPFRLSEALNY
HLKPAPVAIVDAALVDDEWHARFSAVERQYLFRILMRRAPATHDEGQVWQIKHDLDVAAMQAGANMLLGNHDFTTFRSSI
CQAASPVKTLDELRVERVQGLSGPEVHFHVRARSFLHNQVRSFVGTLERVGTGAWSPEDVKHALEAKDRAACGPVCPGHG
LYLARVGYPDPVFSSDRV

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=34.6715328467153, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=37.2, Blast_Score=149, Evalue=2e-37,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=28.8524590163934, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_SILST (Q1GE00)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614378.1
- ProteinModelPortal:   Q1GE00
- SMR:   Q1GE00
- STRING:   Q1GE00
- GeneID:   4076710
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2384
- NMPDR:   fig|292414.1.peg.893
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q1GE00
- ProtClustDB:   PRK00021
- BioCyc:   SSP292414:TM1040_2384-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28681; Mature: 28550

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFALKVEYHGKPFAGWQRQKDQPSVQGAIEAALARIEPGEHTIAAAGRTDTGVHGLGQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCH
VAHCDLEKNWDPFRLSEALNYHLKPAPVAIVDAALVDDEWHARFSAVERQYLFRILMRRA
HEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
PATHDEGQVWQIKHDLDVAAMQAGANMLLGNHDFTTFRSSICQAASPVKTLDELRVERVQ
CCCCCCCCEEEEECCCHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHC
GLSGPEVHFHVRARSFLHNQVRSFVGTLERVGTGAWSPEDVKHALEAKDRAACGPVCPGH
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GLYLARVGYPDPVFSSDRV
CEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PRFALKVEYHGKPFAGWQRQKDQPSVQGAIEAALARIEPGEHTIAAAGRTDTGVHGLGQ
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCH
VAHCDLEKNWDPFRLSEALNYHLKPAPVAIVDAALVDDEWHARFSAVERQYLFRILMRRA
HEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
PATHDEGQVWQIKHDLDVAAMQAGANMLLGNHDFTTFRSSICQAASPVKTLDELRVERVQ
CCCCCCCCEEEEECCCHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHC
GLSGPEVHFHVRARSFLHNQVRSFVGTLERVGTGAWSPEDVKHALEAKDRAACGPVCPGH
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GLYLARVGYPDPVFSSDRV
CEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA