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The map label for this gene is folD

Identifier: 99082196

GI number: 99082196

Start: 2477322

End: 2478224

Strand: Reverse

Name: folD

Synonym: TM1040_2356

Alternate gene names: 99082196

Gene position: 2478224-2477322 (Counterclockwise)

Preceding gene: 99082197

Following gene: 99082182

Centisome position: 77.42

GC content: 60.8

Gene sequence:

>903_bases
ATGGCAGCAGAAATCATTGACGGGAAGGCCTTTGCGGCCAAGGTCCGCGGTCAGGTGACCGAGCATGTGACCCGCCTGAA
AGAAGAACACGGCATCACTCCGGGGCTGGCGGTTGTGCTGGTCGGTGAAGATCCGGCCTCTCAGGTCTATGTGCGCTCCA
AGGGCAAGCAGACCGTTGAGGTGGGCATGAACTCCTACGAGCATAAGCTCGACGCTGACACCTCCGAAGCGGATCTCCTG
GCGCTGATTGACCAGCTCAACAAGGACTCCGAAGTGCATGGGATTCTGGTGCAGCTGCCTCTGCCCAAGCATCTCGACGA
GGATCTGGTGATCAACTCCATCGCGCCGGAAAAAGATGTCGATGGCTTTCATATCTCCAATGTGGGCCTGCTTGGGACGG
GACAAAAATCCATGGTCCCCTGTACGCCGCTTGGTTGTCTGATGATGTTGCGCGACTATCATGGCTCGCTGTCTGGTCTC
GATGCCGTGGTGATTGGCCGCTCCAACATCGTGGGCAAGCCGATGGCTCAGCTCTTGCTGGGGGACAGCTGCACGGTGAC
GATTGCGCATTCGCGGACCAAGGATCTGCCCGAGGTGGTGCGCCGCGCCGACATCGTGGTGGCGGCGGTGGGCCGTCCCG
AGATGGTTCCGGGTGACTGGATCAAGGAAGGTGCAACCGTTATCGACGTTGGCATCAACCGCATCGACGCTCCTGAGAAG
GGCGAGGGCAAGACTCGTCTGGTGGGGGATGTCCACTATGAGAGCGCAGCGGCGCGCGCCGGGGCGATTACACCGGTGCC
CGGCGGGGTTGGTCCGATGACGATTGCCTGTCTTTTGGCGAATACGGTCACGGCCTGCTGTCGTGCGAACAATCTCTCCG
AGCCCGAAGGCCTGACGGCCTGA

Upstream 100 bases:

>100_bases
GCGATATGGTGGATTGGTCGATTTCGCGGGAAGCGCGTGAAATCCCCGAGATTTGAGCCGACTCACAATCGAGTTCGGCC
CCCTGAGAAGGATAGATACG

Downstream 100 bases:

>100_bases
TGCGGCCCTGAGGGGCCGCTGCCTCCGGCGGAGATATTTTTGAAAAGATGAAAAAGGCGTCGGGGCTGTTGCCTCCGGCG
CCTTTTGGTTTTGCAGGGCG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAAEIIDGKAFAAKVRGQVTEHVTRLKEEHGITPGLAVVLVGEDPASQVYVRSKGKQTVEVGMNSYEHKLDADTSEADLL
ALIDQLNKDSEVHGILVQLPLPKHLDEDLVINSIAPEKDVDGFHISNVGLLGTGQKSMVPCTPLGCLMMLRDYHGSLSGL
DAVVIGRSNIVGKPMAQLLLGDSCTVTIAHSRTKDLPEVVRRADIVVAAVGRPEMVPGDWIKEGATVIDVGINRIDAPEK
GEGKTRLVGDVHYESAAARAGAITPVPGGVGPMTIACLLANTVTACCRANNLSEPEGLTA

Sequences:

>Translated_300_residues
MAAEIIDGKAFAAKVRGQVTEHVTRLKEEHGITPGLAVVLVGEDPASQVYVRSKGKQTVEVGMNSYEHKLDADTSEADLL
ALIDQLNKDSEVHGILVQLPLPKHLDEDLVINSIAPEKDVDGFHISNVGLLGTGQKSMVPCTPLGCLMMLRDYHGSLSGL
DAVVIGRSNIVGKPMAQLLLGDSCTVTIAHSRTKDLPEVVRRADIVVAAVGRPEMVPGDWIKEGATVIDVGINRIDAPEK
GEGKTRLVGDVHYESAAARAGAITPVPGGVGPMTIACLLANTVTACCRANNLSEPEGLTA
>Mature_299_residues
AAEIIDGKAFAAKVRGQVTEHVTRLKEEHGITPGLAVVLVGEDPASQVYVRSKGKQTVEVGMNSYEHKLDADTSEADLLA
LIDQLNKDSEVHGILVQLPLPKHLDEDLVINSIAPEKDVDGFHISNVGLLGTGQKSMVPCTPLGCLMMLRDYHGSLSGLD
AVVIGRSNIVGKPMAQLLLGDSCTVTIAHSRTKDLPEVVRRADIVVAAVGRPEMVPGDWIKEGATVIDVGINRIDAPEKG
EGKTRLVGDVHYESAAARAGAITPVPGGVGPMTIACLLANTVTACCRANNLSEPEGLTA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=47.4226804123711, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=44.5945945945946, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI94721354, Length=308, Percent_Identity=44.1558441558442, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI36796743, Length=212, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786741, Length=286, Percent_Identity=54.1958041958042, Blast_Score=281, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=296, Percent_Identity=44.5945945945946, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=293, Percent_Identity=45.0511945392491, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=43.4782608695652, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=317, Percent_Identity=26.4984227129338, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=45.5172413793103, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=45.5172413793103, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=45.5172413793103, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=45.5172413793103, Blast_Score=234, Evalue=4e-62,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=46.0750853242321, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=46.0750853242321, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_SILST (Q1GE28)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614350.1
- ProteinModelPortal:   Q1GE28
- SMR:   Q1GE28
- STRING:   Q1GE28
- GeneID:   4076475
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2356
- NMPDR:   fig|292414.1.peg.920
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q1GE28
- ProtClustDB:   PRK14188
- BioCyc:   SSP292414:TM1040_2356-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31628; Mature: 31497

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEIIDGKAFAAKVRGQVTEHVTRLKEEHGITPGLAVVLVGEDPASQVYVRSKGKQTVE
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCEEEE
VGMNSYEHKLDADTSEADLLALIDQLNKDSEVHGILVQLPLPKHLDEDLVINSIAPEKDV
ECCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCC
DGFHISNVGLLGTGQKSMVPCTPLGCLMMLRDYHGSLSGLDAVVIGRSNIVGKPMAQLLL
CCEEECCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHC
GDSCTVTIAHSRTKDLPEVVRRADIVVAAVGRPEMVPGDWIKEGATVIDVGINRIDAPEK
CCCEEEEEECCCCCCHHHHHHHHCEEEEECCCCCCCCCHHHHCCCEEEEECCCCCCCCCC
GEGKTRLVGDVHYESAAARAGAITPVPGGVGPMTIACLLANTVTACCRANNLSEPEGLTA
CCCCEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AAEIIDGKAFAAKVRGQVTEHVTRLKEEHGITPGLAVVLVGEDPASQVYVRSKGKQTVE
CCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCEEEE
VGMNSYEHKLDADTSEADLLALIDQLNKDSEVHGILVQLPLPKHLDEDLVINSIAPEKDV
ECCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCC
DGFHISNVGLLGTGQKSMVPCTPLGCLMMLRDYHGSLSGLDAVVIGRSNIVGKPMAQLLL
CCEEECCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHC
GDSCTVTIAHSRTKDLPEVVRRADIVVAAVGRPEMVPGDWIKEGATVIDVGINRIDAPEK
CCCEEEEEECCCCCCHHHHHHHHCEEEEECCCCCCCCCHHHHCCCEEEEECCCCCCCCCC
GEGKTRLVGDVHYESAAARAGAITPVPGGVGPMTIACLLANTVTACCRANNLSEPEGLTA
CCCCEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA