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The map label for this gene is thrC [H]

Identifier: 99082170

GI number: 99082170

Start: 2450310

End: 2451701

Strand: Reverse

Name: thrC [H]

Synonym: TM1040_2330

Alternate gene names: 99082170

Gene position: 2451701-2450310 (Counterclockwise)

Preceding gene: 99082171

Following gene: 99082169

Centisome position: 76.59

GC content: 60.78

Gene sequence:

>1392_bases
ATGAAATATATCTCCACCCGCGGCCAAGCGCCCGAACTCACCTTCGAAGAAGCCATGCTGACCGGGCTTGCGCGCGACGG
CGGGCTTTATGTTCCGGCAGAAATCCCGACGCTCTCGGCAGAGGAAATCGCAGGCTTTGCCGGGCTGCCTTATGAGGAGG
TCGCGTTTCGCGTGATGTGGCCCTATGTGTCGGGGTCTTTCTCCGAAGAGGAATTCAAGGGCATCATCGCGCGCGCCTAT
GCCGGGTTTGAGCACGCCGCTCGCGCGCCGCTGAAACAGATGGCGCCGAACCACTTCCTGTTGGAGCTCTTTCACGGCCC
GACGTTGGCGTTCAAGGATTTCGCCATGCAGCTCATCGGTCAGCTGTTTCAGGTCGCGCTCAAACGCCGGGGCGACAGCG
TGACCATCGTGGGTGCCACTTCTGGCGACACCGGGTCCGCGGCGATCGAGGCGTTTCGCGGTCTGGACGCGGTCAACGTC
TTTATCATGTATCCCCATGGCCGCGTCTCCGAGGTGCAGCGCCGCCAGATGACCACACCGCAGGACGCCAATGTGCATGC
CCTCGCGGTGGATGGAGACTTTGACGACTGCCAGGCTGCGGTCAAAGACATGTTCAACGATTTTGACTTCCGCGATTCGG
TGCATCTGGCGGGCGTGAACTCGATCAACTTTGCCCGCGTTTTGGCGCAGGTGGTCTATTACTTCACCGCTGCCGTGGCC
TTGGGCGCACCGCACCGCAAAGTGTCCTTCACCGTGCCGACCGGTAACTTTGGCGACATCTTTGCGGGCTTTATCGCGCG
CCAGATGGGGCTGCCGATCGATCAGCTGGTGGTCGCCACCAACCAGAACGACATCCTGCACCGCTGCCTCTCGGGCGAGG
GCTATTTCAAAGGCGAGACCATCCCGTCGATTTCGCCTTCTATGGATATTCAGGTCTCTTCGAACTTCGAGCGGGCCTTG
TTCTATGCCTACGATCAGGACGGCGCGGCTGTGGCGCAGCTGATGGACGAACTGAAGACCGGTGGTGGTTTTAACGTGAG
CCAGGGGGCCATGCAGGCGTTGAGCGAAATCTACAGCTCAGGCCGCGCTTCCGAGGAGGAGACTTCCGCCACGATCAAAT
CCGAACTCGCGGCCTCAGGAGAGCTGCTTTGCCCACATGGGGCAGTTGGGGTGAAGGTCGCCAATGAACACCTCAAGGAT
GGGGTGCCGATGGTCACGCTGGCCACGGCGCATCCCGCAAAATTCCCGGCCGCGGTCGAGGCGGCCTCGGAGGTGCATCC
GCCTCTTCCCCCTCGCATGGCAGACCTGTATGACAGATCGGAGCGCGTGACCCGGATCGCCAATGATCTCGGCGCGATTG
AAGATCATATCAGAAAGCACATCGCCAATTGA

Upstream 100 bases:

>100_bases
AATATGTCATCACCTGGTTCTCGCTCGCCCTCATATGGGCGGCCATGACCGCCGCCTTTCTCTTGCGTACGCGCGCCAAA
ACCTGACAGGAACTGACGCC

Downstream 100 bases:

>100_bases
CCGTTAAACAAGACACCCTGCCCAACGGGTTTCGTATTGTCACCGAGTATATGCCGGGGCTGCAATCCGCAGCCCTCGGC
ATCTGGGTTTCGGCAGGGGG

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKYISTRGQAPELTFEEAMLTGLARDGGLYVPAEIPTLSAEEIAGFAGLPYEEVAFRVMWPYVSGSFSEEEFKGIIARAY
AGFEHAARAPLKQMAPNHFLLELFHGPTLAFKDFAMQLIGQLFQVALKRRGDSVTIVGATSGDTGSAAIEAFRGLDAVNV
FIMYPHGRVSEVQRRQMTTPQDANVHALAVDGDFDDCQAAVKDMFNDFDFRDSVHLAGVNSINFARVLAQVVYYFTAAVA
LGAPHRKVSFTVPTGNFGDIFAGFIARQMGLPIDQLVVATNQNDILHRCLSGEGYFKGETIPSISPSMDIQVSSNFERAL
FYAYDQDGAAVAQLMDELKTGGGFNVSQGAMQALSEIYSSGRASEEETSATIKSELAASGELLCPHGAVGVKVANEHLKD
GVPMVTLATAHPAKFPAAVEAASEVHPPLPPRMADLYDRSERVTRIANDLGAIEDHIRKHIAN

Sequences:

>Translated_463_residues
MKYISTRGQAPELTFEEAMLTGLARDGGLYVPAEIPTLSAEEIAGFAGLPYEEVAFRVMWPYVSGSFSEEEFKGIIARAY
AGFEHAARAPLKQMAPNHFLLELFHGPTLAFKDFAMQLIGQLFQVALKRRGDSVTIVGATSGDTGSAAIEAFRGLDAVNV
FIMYPHGRVSEVQRRQMTTPQDANVHALAVDGDFDDCQAAVKDMFNDFDFRDSVHLAGVNSINFARVLAQVVYYFTAAVA
LGAPHRKVSFTVPTGNFGDIFAGFIARQMGLPIDQLVVATNQNDILHRCLSGEGYFKGETIPSISPSMDIQVSSNFERAL
FYAYDQDGAAVAQLMDELKTGGGFNVSQGAMQALSEIYSSGRASEEETSATIKSELAASGELLCPHGAVGVKVANEHLKD
GVPMVTLATAHPAKFPAAVEAASEVHPPLPPRMADLYDRSERVTRIANDLGAIEDHIRKHIAN
>Mature_463_residues
MKYISTRGQAPELTFEEAMLTGLARDGGLYVPAEIPTLSAEEIAGFAGLPYEEVAFRVMWPYVSGSFSEEEFKGIIARAY
AGFEHAARAPLKQMAPNHFLLELFHGPTLAFKDFAMQLIGQLFQVALKRRGDSVTIVGATSGDTGSAAIEAFRGLDAVNV
FIMYPHGRVSEVQRRQMTTPQDANVHALAVDGDFDDCQAAVKDMFNDFDFRDSVHLAGVNSINFARVLAQVVYYFTAAVA
LGAPHRKVSFTVPTGNFGDIFAGFIARQMGLPIDQLVVATNQNDILHRCLSGEGYFKGETIPSISPSMDIQVSSNFERAL
FYAYDQDGAAVAQLMDELKTGGGFNVSQGAMQALSEIYSSGRASEEETSATIKSELAASGELLCPHGAVGVKVANEHLKD
GVPMVTLATAHPAKFPAAVEAASEVHPPLPPRMADLYDRSERVTRIANDLGAIEDHIRKHIAN

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI149193323, Length=454, Percent_Identity=35.2422907488987, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI153792148, Length=517, Percent_Identity=32.8820116054159, Blast_Score=246, Evalue=4e-65,
Organism=Escherichia coli, GI1786185, Length=464, Percent_Identity=31.8965517241379, Blast_Score=217, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319901, Length=456, Percent_Identity=44.078947368421, Blast_Score=347, Evalue=3e-96,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 50072; Mature: 50072

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYISTRGQAPELTFEEAMLTGLARDGGLYVPAEIPTLSAEEIAGFAGLPYEEVAFRVMW
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCHHHHHEEEEE
PYVSGSFSEEEFKGIIARAYAGFEHAARAPLKQMAPNHFLLELFHGPTLAFKDFAMQLIG
HHHCCCCCHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCEEHHHHCCCCHHHHHHHHHHHH
QLFQVALKRRGDSVTIVGATSGDTGSAAIEAFRGLDAVNVFIMYPHGRVSEVQRRQMTTP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCC
QDANVHALAVDGDFDDCQAAVKDMFNDFDFRDSVHLAGVNSINFARVLAQVVYYFTAAVA
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LGAPHRKVSFTVPTGNFGDIFAGFIARQMGLPIDQLVVATNQNDILHRCLSGEGYFKGET
HCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHEEEECCCHHHHHHHHCCCCEECCCC
IPSISPSMDIQVSSNFERALFYAYDQDGAAVAQLMDELKTGGGFNVSQGAMQALSEIYSS
CCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GRASEEETSATIKSELAASGELLCPHGAVGVKVANEHLKDGVPMVTLATAHPAKFPAAVE
CCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEECHHHHHCCCCEEEEECCCCCCCCHHHH
AASEVHPPLPPRMADLYDRSERVTRIANDLGAIEDHIRKHIAN
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYISTRGQAPELTFEEAMLTGLARDGGLYVPAEIPTLSAEEIAGFAGLPYEEVAFRVMW
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCHHHHHEEEEE
PYVSGSFSEEEFKGIIARAYAGFEHAARAPLKQMAPNHFLLELFHGPTLAFKDFAMQLIG
HHHCCCCCHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCEEHHHHCCCCHHHHHHHHHHHH
QLFQVALKRRGDSVTIVGATSGDTGSAAIEAFRGLDAVNVFIMYPHGRVSEVQRRQMTTP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCC
QDANVHALAVDGDFDDCQAAVKDMFNDFDFRDSVHLAGVNSINFARVLAQVVYYFTAAVA
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LGAPHRKVSFTVPTGNFGDIFAGFIARQMGLPIDQLVVATNQNDILHRCLSGEGYFKGET
HCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHEEEECCCHHHHHHHHCCCCEECCCC
IPSISPSMDIQVSSNFERALFYAYDQDGAAVAQLMDELKTGGGFNVSQGAMQALSEIYSS
CCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GRASEEETSATIKSELAASGELLCPHGAVGVKVANEHLKDGVPMVTLATAHPAKFPAAVE
CCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEECHHHHHCCCCEEEEECCCCCCCCHHHH
AASEVHPPLPPRMADLYDRSERVTRIANDLGAIEDHIRKHIAN
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1333566; 10984043 [H]