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The map label for this gene is murF [H]

Identifier: 99081859

GI number: 99081859

Start: 2122678

End: 2124069

Strand: Direct

Name: murF [H]

Synonym: TM1040_2019

Alternate gene names: 99081859

Gene position: 2122678-2124069 (Clockwise)

Preceding gene: 99081858

Following gene: 99081860

Centisome position: 66.31

GC content: 66.81

Gene sequence:

>1392_bases
ATGAGCCTTTGGACCGCACAGGAGGCCGCCGCCGCCACCGGAGGACGTGCAACCTCGGACTGGCAGGTGAACGGTATCTC
CATCGACACCCGCACTATTGAACCCGGGGATCTCTTTGTGGCGCTCAAGGCGGCCCGCGATGGGCACGACTTTGTGGCGC
AGGCCCTGGAAAAAGGCGCCGGAGCGGCGCTGGTGTCGCATATCCCCGAAGGCGTGGCCGAGGATGCCCCGCTCCTGCTG
GTGGAGGATGTGCAAACCGCGCTGGAAGCGCTGGGGCGCGCGGGTCGCGCCCGCACAAAGGCGCGCGTGGTGGCGGTGAC
AGGATCGGTGGGCAAGACCTCAACGAAGGAAATGCTCGCCGCGATGCTCACGCCGCAGGGACGCACCCATGCGGCGGTCG
CAAGCTACAACAACCACTGGGGCGTGCCGCTGACACTGGCGCGGATGCCTGCCGACACCGAATATGCCGTGATCGAAATC
GGCATGAACCACCCCGGCGAGATCGCGCCGCTCTCGCGTCAGGCCCGCCCGCATGTGGCGCTGGTGACAACCGTGGCTGC
CGTACATCTTGAGGCCTTTGAGGATGTGGCGGGGATCGCCCGCGAGAAGGCCGCCATCATCGAAGGGCTGGAACCCGGCG
GCATCGCGGTGCTGAACGCCGATCTTGAGGAAACCCCGGTGCTGCGCGACGTGGCACAAGATGCGGGCGTCGCAACGCGC
TGGTTTGGCCAGACGAGTGATGACTACTGCCTGCAGGAGGCGCAGCTGTCGGGCGACGTCACGCGCGCGCGGATCGTACA
GGCGGAGCGTGAATTTTCGGCCGAGATCCAATCGCTTGGCGTACATTTTGCCATGAACGCCACGGGCGCGCTGGCCGCCA
TTGAGGCGCTCGGCGCGGATCTGGAACTGGCGCTTGCCGGGCTTCAGGGCTGGTCGCCGGTCAAAGGGCGCGGCGCGCGC
TTTACCGTGGGGCTGCCCTCCGGTCCCGTCACGGTGCTGGACGACGCCTATAACGCCAACCCCACATCCATCGGGGCGGC
GCTCACGGTTCTTGCCGCGACCCCCACCGAGGGGCGCCGCATCGCCTATCTCGCTGATATGAAAGAACTCGGCCCCGAAG
AGGCGCGACTGCACGCGGGCCTGGCCGATCATCCGGCGCTGGCAAAGATTGACGCGGTACATTGCATCGGTCCCCTGATG
AAACATCTCTACGCGGCCTTGCCGGAGAACAAGCGCGGCACCTGGGTCGAAACCTCGGCCGAGGCCGCCTCTGCCCTGCC
CGCGCAGATCCAAGGGGGCGATGTGGTACTGGCGAAGGGGTCGCTGTCCATGGCGGTGGCCCGCGTGGTTGACGCGATCC
GCGAAATCGGTCAAGGCAACGCACCTTCGTGA

Upstream 100 bases:

>100_bases
AGGGCCATGAGAGCGGCCAGACCATCGGCACCGATGTATTGCCCTTTGACGACGTGGAGCAGGCCAGCATGGCCGTCGCC
GCCCTTGACGGGAGAATGGT

Downstream 100 bases:

>100_bases
CTCGTAATGATAGACCCACTTAAGACAGGACCCGCACTGGCATGCTTTATTGGTTGACCGCACTGTCGGACGGGGGAGAT
TTCTTCAACCTCTTCCGCTA

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSLWTAQEAAAATGGRATSDWQVNGISIDTRTIEPGDLFVALKAARDGHDFVAQALEKGAGAALVSHIPEGVAEDAPLLL
VEDVQTALEALGRAGRARTKARVVAVTGSVGKTSTKEMLAAMLTPQGRTHAAVASYNNHWGVPLTLARMPADTEYAVIEI
GMNHPGEIAPLSRQARPHVALVTTVAAVHLEAFEDVAGIAREKAAIIEGLEPGGIAVLNADLEETPVLRDVAQDAGVATR
WFGQTSDDYCLQEAQLSGDVTRARIVQAEREFSAEIQSLGVHFAMNATGALAAIEALGADLELALAGLQGWSPVKGRGAR
FTVGLPSGPVTVLDDAYNANPTSIGAALTVLAATPTEGRRIAYLADMKELGPEEARLHAGLADHPALAKIDAVHCIGPLM
KHLYAALPENKRGTWVETSAEAASALPAQIQGGDVVLAKGSLSMAVARVVDAIREIGQGNAPS

Sequences:

>Translated_463_residues
MSLWTAQEAAAATGGRATSDWQVNGISIDTRTIEPGDLFVALKAARDGHDFVAQALEKGAGAALVSHIPEGVAEDAPLLL
VEDVQTALEALGRAGRARTKARVVAVTGSVGKTSTKEMLAAMLTPQGRTHAAVASYNNHWGVPLTLARMPADTEYAVIEI
GMNHPGEIAPLSRQARPHVALVTTVAAVHLEAFEDVAGIAREKAAIIEGLEPGGIAVLNADLEETPVLRDVAQDAGVATR
WFGQTSDDYCLQEAQLSGDVTRARIVQAEREFSAEIQSLGVHFAMNATGALAAIEALGADLELALAGLQGWSPVKGRGAR
FTVGLPSGPVTVLDDAYNANPTSIGAALTVLAATPTEGRRIAYLADMKELGPEEARLHAGLADHPALAKIDAVHCIGPLM
KHLYAALPENKRGTWVETSAEAASALPAQIQGGDVVLAKGSLSMAVARVVDAIREIGQGNAPS
>Mature_462_residues
SLWTAQEAAAATGGRATSDWQVNGISIDTRTIEPGDLFVALKAARDGHDFVAQALEKGAGAALVSHIPEGVAEDAPLLLV
EDVQTALEALGRAGRARTKARVVAVTGSVGKTSTKEMLAAMLTPQGRTHAAVASYNNHWGVPLTLARMPADTEYAVIEIG
MNHPGEIAPLSRQARPHVALVTTVAAVHLEAFEDVAGIAREKAAIIEGLEPGGIAVLNADLEETPVLRDVAQDAGVATRW
FGQTSDDYCLQEAQLSGDVTRARIVQAEREFSAEIQSLGVHFAMNATGALAAIEALGADLELALAGLQGWSPVKGRGARF
TVGLPSGPVTVLDDAYNANPTSIGAALTVLAATPTEGRRIAYLADMKELGPEEARLHAGLADHPALAKIDAVHCIGPLMK
HLYAALPENKRGTWVETSAEAASALPAQIQGGDVVLAKGSLSMAVARVVDAIREIGQGNAPS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=37.3303167420815, Blast_Score=194, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48131; Mature: 48000

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLWTAQEAAAATGGRATSDWQVNGISIDTRTIEPGDLFVALKAARDGHDFVAQALEKGA
CCCCCCHHHHHHCCCCCCCCEEEEEEEEECEECCCCCEEEEEEECCCCHHHHHHHHHCCC
GAALVSHIPEGVAEDAPLLLVEDVQTALEALGRAGRARTKARVVAVTGSVGKTSTKEMLA
CHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHH
AMLTPQGRTHAAVASYNNHWGVPLTLARMPADTEYAVIEIGMNHPGEIAPLSRQARPHVA
HHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCH
LVTTVAAVHLEAFEDVAGIAREKAAIIEGLEPGGIAVLNADLEETPVLRDVAQDAGVATR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEE
WFGQTSDDYCLQEAQLSGDVTRARIVQAEREFSAEIQSLGVHFAMNATGALAAIEALGAD
ECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCC
LELALAGLQGWSPVKGRGARFTVGLPSGPVTVLDDAYNANPTSIGAALTVLAATPTEGRR
CEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHEEEEEEECCCCCCE
IAYLADMKELGPEEARLHAGLADHPALAKIDAVHCIGPLMKHLYAALPENKRGTWVETSA
EEEECCHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCH
EAASALPAQIQGGDVVLAKGSLSMAVARVVDAIREIGQGNAPS
HHHHCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLWTAQEAAAATGGRATSDWQVNGISIDTRTIEPGDLFVALKAARDGHDFVAQALEKGA
CCCCCHHHHHHCCCCCCCCEEEEEEEEECEECCCCCEEEEEEECCCCHHHHHHHHHCCC
GAALVSHIPEGVAEDAPLLLVEDVQTALEALGRAGRARTKARVVAVTGSVGKTSTKEMLA
CHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHH
AMLTPQGRTHAAVASYNNHWGVPLTLARMPADTEYAVIEIGMNHPGEIAPLSRQARPHVA
HHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCH
LVTTVAAVHLEAFEDVAGIAREKAAIIEGLEPGGIAVLNADLEETPVLRDVAQDAGVATR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEE
WFGQTSDDYCLQEAQLSGDVTRARIVQAEREFSAEIQSLGVHFAMNATGALAAIEALGAD
ECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCC
LELALAGLQGWSPVKGRGARFTVGLPSGPVTVLDDAYNANPTSIGAALTVLAATPTEGRR
CEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHEEEEEEECCCCCCE
IAYLADMKELGPEEARLHAGLADHPALAKIDAVHCIGPLMKHLYAALPENKRGTWVETSA
EEEECCHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCH
EAASALPAQIQGGDVVLAKGSLSMAVARVVDAIREIGQGNAPS
HHHHCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]