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The map label for this gene is murE [H]

Identifier: 99081858

GI number: 99081858

Start: 2121197

End: 2122681

Strand: Direct

Name: murE [H]

Synonym: TM1040_2018

Alternate gene names: 99081858

Gene position: 2121197-2122681 (Clockwise)

Preceding gene: 99081857

Following gene: 99081859

Centisome position: 66.27

GC content: 66.4

Gene sequence:

>1485_bases
ATGACACAGAGACCAGCGCTCAAGCTCAGCCAGTTGGGACTGACCGCAAGAGCGGGCCTGGACCCGCAGATCACGGGGCT
TGCGGTGGACAGCCGCGAGGTTGGCGAGGGTTTTGTTTTTGCCGCCCTGCCCGGCACGCGCGTGCATGGTGCAACCTTTG
TCGAACAGGTGCTCGATCAGGGCGCGGTGGCCATTCTGACCGACGCCAAGGGCGCCGAGATCGCAGGCGAGGCCATTGCC
GCAGCGGGCGCAGCCCTTGTGGTGGCCGAAGACCCGCGGCAGGCGCTCTCGGGTGCAGCGGCGCTCTGGTTTGGCGCCCA
GCCCCCGGTGATGGCAGCCGTGACAGGCACCAATGGCAAGACCTCCGTGTCGACCTTCCTGCGCATGATCTGGACCGAGC
TTGGCCACAAGGCCGTGAACCTTGGCACCACCGGCATCGAGGGCGCATGGTCACATCCGCTGGCGCATACCACGCCCGAG
CCGATCACCCTGCACCGCGCGCTTGCGGCAGCAGCCGAGGCGGGCGTCACCCATGCGGCGATGGAGGCCTCCTCGCATGG
GCTGGATCAGCGGCGGCTGGACGGTGTACAGCTCTCGGCGGCGGGTTTCACGAATTTCACCCAGGATCACCTCGACTATC
ACGAGACCTTTGAGGCCTATTTTGCGGCCAAGGCAGGGCTTTTCCGTCGTGTGCTCTCGGAAGATGGCGTCGCCGTCATC
AATATGACCGACCCCAAAGGGGCTGAGATGCGCGCCATTGCTGCCGCCCGCGGGCAGGAGATCATTACGGTTGGGCGCGG
TCTGGGTGACATTGCCCTGATGGGTATGCGAGTCGATGCCACCGGGCAGGACATCCGGTTCACATGGCACGACCGCCCCT
TTGCCAAGCGGTTGAACCTCATCGGCGGCTTTCAGGCGGAAAACGTGCTGGTGGCGGCGGGTCTGGCGATTGCCAGCGGC
GAGGACCCCGAGCAGGTGTTTGACACCCTGCCTCACCTCAGCACGGTGCGCGGGCGGATGCAGCTTGCGGCAACCCGCGA
CAATGGCGCGACGGTGTTTGTGGATTACGCCCACACCCCCGACGCGGTTGCCACCGCGATCAAGGCGCTGCGCCCGCATG
TTCTGGGCCGCCTTGTGGCGATCGTCGGCGCGGGCGGGGATCGCGATGCAACCAAACGCCCCTTGATGGGCGCCGCAGCG
CAGGACAATGCCGATGCGGTGATCGTCACCGATGACAACCCCCGCTCTGAAGATCCCGCCGCCATTCGCGCGGCCGTCAT
GGGCGGCGCGCCGGACGCGCTCAATGTGGGCGACCGCGCCGAAGCGATCCTGCGCGGCGTCGATATGCTCGAGGCTGGCG
ATGCGCTCCTCATCTGCGGCAAGGGCCATGAGAGCGGCCAGACCATCGGCACCGATGTATTGCCCTTTGACGACGTGGAG
CAGGCCAGCATGGCCGTCGCCGCCCTTGACGGGAGAATGGTATGA

Upstream 100 bases:

>100_bases
TGATCGCGCGCCTTGCGCCGCTCCTTGGGCTCAGACCACAGATTGAACCTGCCCGTATCTCTGGTGTAACCCTCACCTCA
AACTAACAGGTGGGGACGCC

Downstream 100 bases:

>100_bases
GCCTTTGGACCGCACAGGAGGCCGCCGCCGCCACCGGAGGACGTGCAACCTCGGACTGGCAGGTGAACGGTATCTCCATC
GACACCCGCACTATTGAACC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTQRPALKLSQLGLTARAGLDPQITGLAVDSREVGEGFVFAALPGTRVHGATFVEQVLDQGAVAILTDAKGAEIAGEAIA
AAGAALVVAEDPRQALSGAAALWFGAQPPVMAAVTGTNGKTSVSTFLRMIWTELGHKAVNLGTTGIEGAWSHPLAHTTPE
PITLHRALAAAAEAGVTHAAMEASSHGLDQRRLDGVQLSAAGFTNFTQDHLDYHETFEAYFAAKAGLFRRVLSEDGVAVI
NMTDPKGAEMRAIAAARGQEIITVGRGLGDIALMGMRVDATGQDIRFTWHDRPFAKRLNLIGGFQAENVLVAAGLAIASG
EDPEQVFDTLPHLSTVRGRMQLAATRDNGATVFVDYAHTPDAVATAIKALRPHVLGRLVAIVGAGGDRDATKRPLMGAAA
QDNADAVIVTDDNPRSEDPAAIRAAVMGGAPDALNVGDRAEAILRGVDMLEAGDALLICGKGHESGQTIGTDVLPFDDVE
QASMAVAALDGRMV

Sequences:

>Translated_494_residues
MTQRPALKLSQLGLTARAGLDPQITGLAVDSREVGEGFVFAALPGTRVHGATFVEQVLDQGAVAILTDAKGAEIAGEAIA
AAGAALVVAEDPRQALSGAAALWFGAQPPVMAAVTGTNGKTSVSTFLRMIWTELGHKAVNLGTTGIEGAWSHPLAHTTPE
PITLHRALAAAAEAGVTHAAMEASSHGLDQRRLDGVQLSAAGFTNFTQDHLDYHETFEAYFAAKAGLFRRVLSEDGVAVI
NMTDPKGAEMRAIAAARGQEIITVGRGLGDIALMGMRVDATGQDIRFTWHDRPFAKRLNLIGGFQAENVLVAAGLAIASG
EDPEQVFDTLPHLSTVRGRMQLAATRDNGATVFVDYAHTPDAVATAIKALRPHVLGRLVAIVGAGGDRDATKRPLMGAAA
QDNADAVIVTDDNPRSEDPAAIRAAVMGGAPDALNVGDRAEAILRGVDMLEAGDALLICGKGHESGQTIGTDVLPFDDVE
QASMAVAALDGRMV
>Mature_493_residues
TQRPALKLSQLGLTARAGLDPQITGLAVDSREVGEGFVFAALPGTRVHGATFVEQVLDQGAVAILTDAKGAEIAGEAIAA
AGAALVVAEDPRQALSGAAALWFGAQPPVMAAVTGTNGKTSVSTFLRMIWTELGHKAVNLGTTGIEGAWSHPLAHTTPEP
ITLHRALAAAAEAGVTHAAMEASSHGLDQRRLDGVQLSAAGFTNFTQDHLDYHETFEAYFAAKAGLFRRVLSEDGVAVIN
MTDPKGAEMRAIAAARGQEIITVGRGLGDIALMGMRVDATGQDIRFTWHDRPFAKRLNLIGGFQAENVLVAAGLAIASGE
DPEQVFDTLPHLSTVRGRMQLAATRDNGATVFVDYAHTPDAVATAIKALRPHVLGRLVAIVGAGGDRDATKRPLMGAAAQ
DNADAVIVTDDNPRSEDPAAIRAAVMGGAPDALNVGDRAEAILRGVDMLEAGDALLICGKGHESGQTIGTDVLPFDDVEQ
ASMAVAALDGRMV

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=467, Percent_Identity=34.0471092077088, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51309; Mature: 51177

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRPALKLSQLGLTARAGLDPQITGLAVDSREVGEGFVFAALPGTRVHGATFVEQVLDQ
CCCCCCCHHHHCCCEEECCCCCCEEEEEECCHHCCCCEEEEECCCCCCCHHHHHHHHHCC
GAVAILTDAKGAEIAGEAIAAAGAALVVAEDPRQALSGAAALWFGAQPPVMAAVTGTNGK
CCEEEEECCCCCHHHHHHHHHCCEEEEEECCHHHHHCCCEEEEECCCCCEEEEEECCCCC
TSVSTFLRMIWTELGHKAVNLGTTGIEGAWSHPLAHTTPEPITLHRALAAAAEAGVTHAA
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
MEASSHGLDQRRLDGVQLSAAGFTNFTQDHLDYHETFEAYFAAKAGLFRRVLSEDGVAVI
HHHHHCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NMTDPKGAEMRAIAAARGQEIITVGRGLGDIALMGMRVDATGQDIRFTWHDRPFAKRLNL
EECCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHH
IGGFQAENVLVAAGLAIASGEDPEQVFDTLPHLSTVRGRMQLAATRDNGATVFVDYAHTP
HCCCCCCCEEEEECEEEECCCCHHHHHHHCCHHHHHHCCEEEEEECCCCCEEEEEECCCC
DAVATAIKALRPHVLGRLVAIVGAGGDRDATKRPLMGAAAQDNADAVIVTDDNPRSEDPA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCH
AIRAAVMGGAPDALNVGDRAEAILRGVDMLEAGDALLICGKGHESGQTIGTDVLPFDDVE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHH
QASMAVAALDGRMV
HHHHEEEECCCCCC
>Mature Secondary Structure 
TQRPALKLSQLGLTARAGLDPQITGLAVDSREVGEGFVFAALPGTRVHGATFVEQVLDQ
CCCCCCHHHHCCCEEECCCCCCEEEEEECCHHCCCCEEEEECCCCCCCHHHHHHHHHCC
GAVAILTDAKGAEIAGEAIAAAGAALVVAEDPRQALSGAAALWFGAQPPVMAAVTGTNGK
CCEEEEECCCCCHHHHHHHHHCCEEEEEECCHHHHHCCCEEEEECCCCCEEEEEECCCCC
TSVSTFLRMIWTELGHKAVNLGTTGIEGAWSHPLAHTTPEPITLHRALAAAAEAGVTHAA
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
MEASSHGLDQRRLDGVQLSAAGFTNFTQDHLDYHETFEAYFAAKAGLFRRVLSEDGVAVI
HHHHHCCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NMTDPKGAEMRAIAAARGQEIITVGRGLGDIALMGMRVDATGQDIRFTWHDRPFAKRLNL
EECCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHH
IGGFQAENVLVAAGLAIASGEDPEQVFDTLPHLSTVRGRMQLAATRDNGATVFVDYAHTP
HCCCCCCCEEEEECEEEECCCCHHHHHHHCCHHHHHHCCEEEEEECCCCCEEEEEECCCC
DAVATAIKALRPHVLGRLVAIVGAGGDRDATKRPLMGAAAQDNADAVIVTDDNPRSEDPA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCH
AIRAAVMGGAPDALNVGDRAEAILRGVDMLEAGDALLICGKGHESGQTIGTDVLPFDDVE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHH
QASMAVAALDGRMV
HHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA