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The map label for this gene is nfdA [H]

Identifier: 99081840

GI number: 99081840

Start: 2103846

End: 2105762

Strand: Reverse

Name: nfdA [H]

Synonym: TM1040_2000

Alternate gene names: 99081840

Gene position: 2105762-2103846 (Counterclockwise)

Preceding gene: 99081845

Following gene: 99081836

Centisome position: 65.79

GC content: 62.7

Gene sequence:

>1917_bases
ATGAGGACCTTGAACCGCAGAAATCTGTTGAAGGGCAGCGTTGCGGCGGCCTGTTTGACGGTGCCCGCCGTGCGCGCAGT
TGGGCAGACGAACTCGCTCTCGGAGATCGGCAAGTTGTTTGCACCGGTGCCTCGGGCAAAGATCTTCACCGCGCGCGATA
TTGTCACGCTTGACCCTTCTCAACCCTCGGCCGAGGCCATCGCGGTTGTCGGCACGCGAATCCTCGCGGTGGGCGCGCTC
AGCGAGGTGCAGGAGATGCTCGGGGATCAACCCTTTGATGTGGATGACAGTTTCGCCGACAAGGTCATCGTGCCAGGGTT
TATCAGCCAGCATGATCATCCGGTTCTGGCGGCGCTGTCGATGTCGTCCGAGATCCTGTCGATCGAGGAGTGGGCCTTGC
CGACGGGCACCGTGCCTGCGGTCAAGGACAAGGCGGACTTCATGAAACGCCTCACGGCAGCCGTGGAGGCGCGTACCACC
CCCGGAGAGCCGGTGGTGACCTGGGGGTATCATCCCGCCTTTTACGGTCCTTTGACACGCGCGGATCTCGACGGGATCAG
CACCGAGCGCCCCATTCTGGTGTGGGGTCGCTCCTGCCACGAGATGATCCTCAATAGCGCCGCCCTGACGGCCGGTGGGG
TGACACAGGCCGCGGTGGAGGCCTTCGACGCGGCCTCGCAGAAACAGGCCAACCTTGCGGAGGGCCATTTCTGGGAGCAG
GGGCTTTTTGCGGTTCTGCCTCATATTGCGTCTTTGGTGGCCACGCCCGAACAGCTGCGCGCTGGGCTCGAACTCAGCCG
TGACTTCATGCACAGCAAGGGCATCACCTTTGGCAACGAGCCCGGCGGCATTCTTGCAAAACCCGTGCAGGACGGAGTCA
ATGCGGTGTTTTCCAGCCCGGATATGCCGTTTCGCTGGTCGTTCATGGTCGATGCCAAGAGCATGGTCGCCAGTTACGCC
GATGACGGCGAGGTCATTGCCCGGTCAGAGGCGCTTCAATCCTGGTACTACGGGATGACAAGCCTCGCGCCGCGCCAGGC
CAAACTGTTTTCGGATGGGGCGATCTATTCGCAGCTCATGCAGGTGCGCGCGCCCTATCTCGACGATCACCACGGCGAGT
GGATGATGGAGAAAGAGCTGTTTGAGCGCGCGTTCAGGGTCTACTGGGATGCGGGGTATCAGCTGCACATCCATGTCAAC
GGTGATGCCGGGTTGGATCGTGTGCTGGAGACGCTCGAGACCAACATGCGTCGCAATCCGCGTTTTGATCACCGTACGGT
CATCGTGCATTTTGCCGTCAGCGCCTTTGACCAGGTGGAGCGGATCAAGGCGCTCGGGGCCATCGTGAGCGGCAATCCCT
ATTATGTCACGGCGCTTGCGGATCAGTATTCCGAAGTGGGTCTTGGCGCCGAGCGGGCAGACAGCATGGTGCGTCTCGGG
GATCTCTCGCGGGCCGGGGTGCGTTGGTCGCTCCATTCGGATATGCCGATGGCGCCAGCTGACCCGTTGTTCCTGATGTG
GTGCGCGGTGAACCGGGTGACCACGTCGGGCCGGGTGGCGGCGCCGGAACAGGCGGTGTCCGCCGAGGACGCGCTGCGCG
GCGTCACCATCGAAGCGGCCTATTCGCTGCAGATGGAAGAAGAAATCGGCAGCCTCGTGCGTGGCAAGCGGGCGAATATG
ACGATCCTTGCCGAGAACCCGCTCGAGGTGGATCCCATGGCGATCCGCGAGATCGAGGTCTGGGGCACGGTGATGGAGGG
GCGGGTGCTGCCGGTCCGTTCGAGTGATCGAGCCGCAGCAACGCAGCAGGACGCCTCGGCTGGTCCGCGTGAGGCGGTTT
CACCAAATGGAGCGCCAGCCTTTGACAAAGCTGCCCTTGAGCATGCCCTGAAGGTCACGCACGCGCATCATCTCTGA

Upstream 100 bases:

>100_bases
ACTTTGCCGCGCTCCGGCATTGGGCGACACGCTGTGACGCTGCGCCCGATTTTGAAAGACCAAAGATATTTTCGAGCGAC
ATCTTCTTGGGAGATATGAC

Downstream 100 bases:

>100_bases
TACGGCGCGTGCGGTGTTCTCTTTCAGGCCTTGCAATTTTTTGGGCGCATCTGCGGCAAGGCGGAAAGCGAACACTTGTG
GTGCCTCGGCGCGCGCGTTC

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MRTLNRRNLLKGSVAAACLTVPAVRAVGQTNSLSEIGKLFAPVPRAKIFTARDIVTLDPSQPSAEAIAVVGTRILAVGAL
SEVQEMLGDQPFDVDDSFADKVIVPGFISQHDHPVLAALSMSSEILSIEEWALPTGTVPAVKDKADFMKRLTAAVEARTT
PGEPVVTWGYHPAFYGPLTRADLDGISTERPILVWGRSCHEMILNSAALTAGGVTQAAVEAFDAASQKQANLAEGHFWEQ
GLFAVLPHIASLVATPEQLRAGLELSRDFMHSKGITFGNEPGGILAKPVQDGVNAVFSSPDMPFRWSFMVDAKSMVASYA
DDGEVIARSEALQSWYYGMTSLAPRQAKLFSDGAIYSQLMQVRAPYLDDHHGEWMMEKELFERAFRVYWDAGYQLHIHVN
GDAGLDRVLETLETNMRRNPRFDHRTVIVHFAVSAFDQVERIKALGAIVSGNPYYVTALADQYSEVGLGAERADSMVRLG
DLSRAGVRWSLHSDMPMAPADPLFLMWCAVNRVTTSGRVAAPEQAVSAEDALRGVTIEAAYSLQMEEEIGSLVRGKRANM
TILAENPLEVDPMAIREIEVWGTVMEGRVLPVRSSDRAAATQQDASAGPREAVSPNGAPAFDKAALEHALKVTHAHHL

Sequences:

>Translated_638_residues
MRTLNRRNLLKGSVAAACLTVPAVRAVGQTNSLSEIGKLFAPVPRAKIFTARDIVTLDPSQPSAEAIAVVGTRILAVGAL
SEVQEMLGDQPFDVDDSFADKVIVPGFISQHDHPVLAALSMSSEILSIEEWALPTGTVPAVKDKADFMKRLTAAVEARTT
PGEPVVTWGYHPAFYGPLTRADLDGISTERPILVWGRSCHEMILNSAALTAGGVTQAAVEAFDAASQKQANLAEGHFWEQ
GLFAVLPHIASLVATPEQLRAGLELSRDFMHSKGITFGNEPGGILAKPVQDGVNAVFSSPDMPFRWSFMVDAKSMVASYA
DDGEVIARSEALQSWYYGMTSLAPRQAKLFSDGAIYSQLMQVRAPYLDDHHGEWMMEKELFERAFRVYWDAGYQLHIHVN
GDAGLDRVLETLETNMRRNPRFDHRTVIVHFAVSAFDQVERIKALGAIVSGNPYYVTALADQYSEVGLGAERADSMVRLG
DLSRAGVRWSLHSDMPMAPADPLFLMWCAVNRVTTSGRVAAPEQAVSAEDALRGVTIEAAYSLQMEEEIGSLVRGKRANM
TILAENPLEVDPMAIREIEVWGTVMEGRVLPVRSSDRAAATQQDASAGPREAVSPNGAPAFDKAALEHALKVTHAHHL
>Mature_638_residues
MRTLNRRNLLKGSVAAACLTVPAVRAVGQTNSLSEIGKLFAPVPRAKIFTARDIVTLDPSQPSAEAIAVVGTRILAVGAL
SEVQEMLGDQPFDVDDSFADKVIVPGFISQHDHPVLAALSMSSEILSIEEWALPTGTVPAVKDKADFMKRLTAAVEARTT
PGEPVVTWGYHPAFYGPLTRADLDGISTERPILVWGRSCHEMILNSAALTAGGVTQAAVEAFDAASQKQANLAEGHFWEQ
GLFAVLPHIASLVATPEQLRAGLELSRDFMHSKGITFGNEPGGILAKPVQDGVNAVFSSPDMPFRWSFMVDAKSMVASYA
DDGEVIARSEALQSWYYGMTSLAPRQAKLFSDGAIYSQLMQVRAPYLDDHHGEWMMEKELFERAFRVYWDAGYQLHIHVN
GDAGLDRVLETLETNMRRNPRFDHRTVIVHFAVSAFDQVERIKALGAIVSGNPYYVTALADQYSEVGLGAERADSMVRLG
DLSRAGVRWSLHSDMPMAPADPLFLMWCAVNRVTTSGRVAAPEQAVSAEDALRGVTIEAAYSLQMEEEIGSLVRGKRANM
TILAENPLEVDPMAIREIEVWGTVMEGRVLPVRSSDRAAATQQDASAGPREAVSPNGAPAFDKAALEHALKVTHAHHL

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 69351; Mature: 69351

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLNRRNLLKGSVAAACLTVPAVRAVGQTNSLSEIGKLFAPVPRAKIFTARDIVTLDPS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCEEEECCC
QPSAEAIAVVGTRILAVGALSEVQEMLGDQPFDVDDSFADKVIVPGFISQHDHPVLAALS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEECCCHHCCCCCCEEEEEH
MSSEILSIEEWALPTGTVPAVKDKADFMKRLTAAVEARTTPGEPVVTWGYHPAFYGPLTR
HCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHCCCCCC
ADLDGISTERPILVWGRSCHEMILNSAALTAGGVTQAAVEAFDAASQKQANLAEGHFWEQ
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
GLFAVLPHIASLVATPEQLRAGLELSRDFMHSKGITFGNEPGGILAKPVQDGVNAVFSSP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHCCC
DMPFRWSFMVDAKSMVASYADDGEVIARSEALQSWYYGMTSLAPRQAKLFSDGAIYSQLM
CCCEEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHH
QVRAPYLDDHHGEWMMEKELFERAFRVYWDAGYQLHIHVNGDAGLDRVLETLETNMRRNP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCC
RFDHRTVIVHFAVSAFDQVERIKALGAIVSGNPYYVTALADQYSEVGLGAERADSMVRLG
CCCCEEEEEHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHHHHHHCCCHHHHHHHHHHC
DLSRAGVRWSLHSDMPMAPADPLFLMWCAVNRVTTSGRVAAPEQAVSAEDALRGVTIEAA
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHCCEEEEHH
YSLQMEEEIGSLVRGKRANMTILAENPLEVDPMAIREIEVWGTVMEGRVLPVRSSDRAAA
EEEEEHHHHHHHHCCCCCCEEEEECCCCCCCCHHHEEEEEHHHHHCCEEEEECCCCCCHH
TQQDASAGPREAVSPNGAPAFDKAALEHALKVTHAHHL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTLNRRNLLKGSVAAACLTVPAVRAVGQTNSLSEIGKLFAPVPRAKIFTARDIVTLDPS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCEEEECCC
QPSAEAIAVVGTRILAVGALSEVQEMLGDQPFDVDDSFADKVIVPGFISQHDHPVLAALS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEECCCHHCCCCCCEEEEEH
MSSEILSIEEWALPTGTVPAVKDKADFMKRLTAAVEARTTPGEPVVTWGYHPAFYGPLTR
HCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHCCCCCC
ADLDGISTERPILVWGRSCHEMILNSAALTAGGVTQAAVEAFDAASQKQANLAEGHFWEQ
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
GLFAVLPHIASLVATPEQLRAGLELSRDFMHSKGITFGNEPGGILAKPVQDGVNAVFSSP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHCCC
DMPFRWSFMVDAKSMVASYADDGEVIARSEALQSWYYGMTSLAPRQAKLFSDGAIYSQLM
CCCEEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHH
QVRAPYLDDHHGEWMMEKELFERAFRVYWDAGYQLHIHVNGDAGLDRVLETLETNMRRNP
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCC
RFDHRTVIVHFAVSAFDQVERIKALGAIVSGNPYYVTALADQYSEVGLGAERADSMVRLG
CCCCEEEEEHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHHHHHHCCCHHHHHHHHHHC
DLSRAGVRWSLHSDMPMAPADPLFLMWCAVNRVTTSGRVAAPEQAVSAEDALRGVTIEAA
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHCCEEEEHH
YSLQMEEEIGSLVRGKRANMTILAENPLEVDPMAIREIEVWGTVMEGRVLPVRSSDRAAA
EEEEEHHHHHHHHCCCCCCEEEEECCCCCCCCHHHEEEEEHHHHHCCEEEEECCCCCCHH
TQQDASAGPREAVSPNGAPAFDKAALEHALKVTHAHHL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA