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The map label for this gene is gcvA [H]

Identifier: 99081845

GI number: 99081845

Start: 2110423

End: 2111313

Strand: Reverse

Name: gcvA [H]

Synonym: TM1040_2005

Alternate gene names: 99081845

Gene position: 2111313-2110423 (Counterclockwise)

Preceding gene: 99081848

Following gene: 99081840

Centisome position: 65.96

GC content: 63.97

Gene sequence:

>891_bases
ATGGATCATCTACCCCCTTTGCGCCTGCTTGTGACCTTTGAGGCGATCTCGCGTCACGGGTCGATGCGCGATGCTGCGCA
GCGGCTCAATGTGACCCAACCGGCGGTCAGTCAGGCGCTCAAGGCGCTGGAGGATCACGTGGGCGTGGCGCTCTTTGATC
GCAGTGTGCGCCCGGCGCGTCTGACCGACGCCGGAGAGATGCTCTCGCGGGCGGTACGCGAGGGGCTTGGCCGGATTGCG
GCCACGCTCGAAGACATCCGTGCCCTGCATGCCATTGGCGAACGGCAGGTAACGGTTTCATGCACATTGGGCATGGCGAC
CTATTGGTTGATGCCGAAATTGCCAATGTTTTATGCGGACTACCCGGAGCTTACGGTCAACGTGCAAGCCCCGGCAACCG
ATCTTCCGGTGCTGAGCCCAGGGATAGATGTGGCGCTTCGCTATGGCCGGGGCGGCTGGCGCGAAGGGCGCACGGAGAAG
CTCTTTGCTGAGGTGATCTGCCCGGTTGGCGCGCCGGCACTGGTGGAGCGCCTTCTTGCGGAGGGCGCCACATTGGCTGA
GGCGCCGTTGATCCATGTGCGCAATCCGCACAATGACCACTGGGCGGGTTGGGAAGATTATCTTCAGGCTCGCGGTATTC
AGCGTCCGCGCGGGGGCGGACAGATCTTTAACAATTACGTCCAGGCGGCGCAGGCGATCTATGATGGGCGCGGGCTTATG
CTGGGCTGGCGCTCGATCACCGGTCAGGCGGAACGCGACGGCGCCCTCCGGGCCTGGCCCGATGGCGCGCTGGATCTCGG
GACGAGCTATTTTGTCACCATGCCCACGACGCCCTCGCAGGACACGCTTCTGTTTGTCGATTGGGTCCGCAAGGCGGCCG
CGACCGTCTAG

Upstream 100 bases:

>100_bases
CTTTCCTGAGGAGTGTCTTTGCCGCAGTATTTCACCACGGGAGGGCTTGACGACAGCCTGTTTCGGGTGAAGATAACTTA
ATCTAAGGATTAAGTTAGAG

Downstream 100 bases:

>100_bases
AGCCTCTGCGTCAGCAGCCAGTCCACAAGTTTGCGCAGACCCGGGTTTTCCCTCAGGTCGTCGCGCTCGACCAGAACAAA
GCTCTCCTGCATCGGCAGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDHLPPLRLLVTFEAISRHGSMRDAAQRLNVTQPAVSQALKALEDHVGVALFDRSVRPARLTDAGEMLSRAVREGLGRIA
ATLEDIRALHAIGERQVTVSCTLGMATYWLMPKLPMFYADYPELTVNVQAPATDLPVLSPGIDVALRYGRGGWREGRTEK
LFAEVICPVGAPALVERLLAEGATLAEAPLIHVRNPHNDHWAGWEDYLQARGIQRPRGGGQIFNNYVQAAQAIYDGRGLM
LGWRSITGQAERDGALRAWPDGALDLGTSYFVTMPTTPSQDTLLFVDWVRKAAATV

Sequences:

>Translated_296_residues
MDHLPPLRLLVTFEAISRHGSMRDAAQRLNVTQPAVSQALKALEDHVGVALFDRSVRPARLTDAGEMLSRAVREGLGRIA
ATLEDIRALHAIGERQVTVSCTLGMATYWLMPKLPMFYADYPELTVNVQAPATDLPVLSPGIDVALRYGRGGWREGRTEK
LFAEVICPVGAPALVERLLAEGATLAEAPLIHVRNPHNDHWAGWEDYLQARGIQRPRGGGQIFNNYVQAAQAIYDGRGLM
LGWRSITGQAERDGALRAWPDGALDLGTSYFVTMPTTPSQDTLLFVDWVRKAAATV
>Mature_296_residues
MDHLPPLRLLVTFEAISRHGSMRDAAQRLNVTQPAVSQALKALEDHVGVALFDRSVRPARLTDAGEMLSRAVREGLGRIA
ATLEDIRALHAIGERQVTVSCTLGMATYWLMPKLPMFYADYPELTVNVQAPATDLPVLSPGIDVALRYGRGGWREGRTEK
LFAEVICPVGAPALVERLLAEGATLAEAPLIHVRNPHNDHWAGWEDYLQARGIQRPRGGGQIFNNYVQAAQAIYDGRGLM
LGWRSITGQAERDGALRAWPDGALDLGTSYFVTMPTTPSQDTLLFVDWVRKAAATV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=251, Percent_Identity=34.6613545816733, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=26, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI157672245, Length=125, Percent_Identity=40, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786448, Length=252, Percent_Identity=29.3650793650794, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.9672131147541, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786401, Length=168, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787128, Length=138, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789639, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32459; Mature: 32459

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLPPLRLLVTFEAISRHGSMRDAAQRLNVTQPAVSQALKALEDHVGVALFDRSVRPAR
CCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCC
LTDAGEMLSRAVREGLGRIAATLEDIRALHAIGERQVTVSCTLGMATYWLMPKLPMFYAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCEECC
YPELTVNVQAPATDLPVLSPGIDVALRYGRGGWREGRTEKLFAEVICPVGAPALVERLLA
CCCEEEEEECCCCCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
EGATLAEAPLIHVRNPHNDHWAGWEDYLQARGIQRPRGGGQIFNNYVQAAQAIYDGRGLM
CCCCHHCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
LGWRSITGQAERDGALRAWPDGALDLGTSYFVTMPTTPSQDTLLFVDWVRKAAATV
EEEHHHCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHCCC
>Mature Secondary Structure
MDHLPPLRLLVTFEAISRHGSMRDAAQRLNVTQPAVSQALKALEDHVGVALFDRSVRPAR
CCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCC
LTDAGEMLSRAVREGLGRIAATLEDIRALHAIGERQVTVSCTLGMATYWLMPKLPMFYAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCEECC
YPELTVNVQAPATDLPVLSPGIDVALRYGRGGWREGRTEKLFAEVICPVGAPALVERLLA
CCCEEEEEECCCCCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
EGATLAEAPLIHVRNPHNDHWAGWEDYLQARGIQRPRGGGQIFNNYVQAAQAIYDGRGLM
CCCCHHCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
LGWRSITGQAERDGALRAWPDGALDLGTSYFVTMPTTPSQDTLLFVDWVRKAAATV
EEEHHHCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]