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The map label for this gene is purD [H]

Identifier: 99081787

GI number: 99081787

Start: 2050013

End: 2051275

Strand: Direct

Name: purD [H]

Synonym: TM1040_1946

Alternate gene names: 99081787

Gene position: 2050013-2051275 (Clockwise)

Preceding gene: 99081785

Following gene: 99081790

Centisome position: 64.04

GC content: 62.87

Gene sequence:

>1263_bases
ATGAATATCCTCATCCTCGGCAGCGGCGGGCGGGAACATGCACTTGCCTGGGCGGTGATGCAGAACCCCAAATGCGACCG
GCTCATTGTGGCGCCGGGCAATGCGGGTATCGCCCAGATCGCCGACTGCGCCAGCCTCAACGCCGAGGATGGCGGCGCGG
TTGTGACATTCGCCGAGGAAAACGCCATTGATTTTGTGATCGTCGGCCCCGAGGCGCCTTTGGCCGCCGGTGTGGCAGAC
CGGTTGCGCGATGCGGGCATTCTGGTCTTTGGTCCCTCTGAGGCCGCCGCCCGGCTGGAGGCTTCCAAAAGCTTCACCAA
GGAGATCTGCGACGCGGCCAATGCGCCCACTGCGGGCTATGGCCACTTCACTGATGCCGAGGCGGCCAAGGCCCATGTCC
GTGCCAACGGCGCGCCGATTGTGGTCAAGGCCGATGGTCTGGCCGCAGGCAAGGGCGTGATCGTGGCGATGGACGAGCAG
ACTGCGCTCGATGCCATCGACGATATGTTCGGCGGTGCCTTTGGTGGGGCGGGCGCAGAGGTTGTCATCGAGGAATTCAT
GGAAGGTGAAGAGGCATCGCTCTTTGTGCTCTGTGATGGTGAGGAAATCCTGTCCATCGGTACCGCACAGGACCACAAGC
GCGTCGGCGAAGGCGACACTGGCCTAAATACCGGCGGCATGGGGGCTTATTCTCCTGCACCGGTTCTGAGCGCCGAGGTT
GAAGCCAAGGCCATGGAAGAGATCGTGAAGCCCACCATGCGGGTGATGGCCGAGCGTGGCATGCCCTACCAAGGCGTGCT
CTATGCAGGCCTGATGATCAAGGACGGCCAGCCGCGTCTGGTGGAATATAACGTCCGCTTTGGCGATCCCGAATGTCAGG
TGCTGATGATGCGCCTTGGCGCGCAGGCCCTGGACCTGATGCAAGCCGCAGCCGAAGGTCGCCTTGCGGACGCCCGCGTC
AACTGGGCTGATGACCACGCGATCACGGTGGTGATGGCTGCGGCAGGCTATCCGGGAAGCTATGAAAAAGGCAGCGAGAT
CAAGGGCCTTGATGCTCTGCCCGAAGACAGCATGAATATGGTCTTTCACGCAGGGACCAAGGCCGATGGCGACAAGATCC
TCGCCAATGGTGGCCGGGTGCTGAATGTGACTGCACGGGGCGAGAGCCTCTCTGAGGCGCGCGATCGCGCCTATGCCATG
GTCGATCAGATCGACTGGCCCGAGGGCTTCGTGCGCCGCGACATCGGCTGGCGCGCGCTTTGA

Upstream 100 bases:

>100_bases
TGCCACCAGAGGGCGCCCCCGGCAAGGGTGTTCTTGTTTATGGGACGCACTCAGCATAGAACGCCCATCAACCAAGGCCA
AGCAGATCGGAGAATGGCTC

Downstream 100 bases:

>100_bases
CCCCGCGATGAATCGCACACCGGTTCAGATTGCCCCGGACAGCCCGCAGTTGCGGGAGGTTCTGGACCTGATCCGGCAGT
GCTTTGCCTATATGGACGGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNILILGSGGREHALAWAVMQNPKCDRLIVAPGNAGIAQIADCASLNAEDGGAVVTFAEENAIDFVIVGPEAPLAAGVAD
RLRDAGILVFGPSEAAARLEASKSFTKEICDAANAPTAGYGHFTDAEAAKAHVRANGAPIVVKADGLAAGKGVIVAMDEQ
TALDAIDDMFGGAFGGAGAEVVIEEFMEGEEASLFVLCDGEEILSIGTAQDHKRVGEGDTGLNTGGMGAYSPAPVLSAEV
EAKAMEEIVKPTMRVMAERGMPYQGVLYAGLMIKDGQPRLVEYNVRFGDPECQVLMMRLGAQALDLMQAAAEGRLADARV
NWADDHAITVVMAAAGYPGSYEKGSEIKGLDALPEDSMNMVFHAGTKADGDKILANGGRVLNVTARGESLSEARDRAYAM
VDQIDWPEGFVRRDIGWRAL

Sequences:

>Translated_420_residues
MNILILGSGGREHALAWAVMQNPKCDRLIVAPGNAGIAQIADCASLNAEDGGAVVTFAEENAIDFVIVGPEAPLAAGVAD
RLRDAGILVFGPSEAAARLEASKSFTKEICDAANAPTAGYGHFTDAEAAKAHVRANGAPIVVKADGLAAGKGVIVAMDEQ
TALDAIDDMFGGAFGGAGAEVVIEEFMEGEEASLFVLCDGEEILSIGTAQDHKRVGEGDTGLNTGGMGAYSPAPVLSAEV
EAKAMEEIVKPTMRVMAERGMPYQGVLYAGLMIKDGQPRLVEYNVRFGDPECQVLMMRLGAQALDLMQAAAEGRLADARV
NWADDHAITVVMAAAGYPGSYEKGSEIKGLDALPEDSMNMVFHAGTKADGDKILANGGRVLNVTARGESLSEARDRAYAM
VDQIDWPEGFVRRDIGWRAL
>Mature_420_residues
MNILILGSGGREHALAWAVMQNPKCDRLIVAPGNAGIAQIADCASLNAEDGGAVVTFAEENAIDFVIVGPEAPLAAGVAD
RLRDAGILVFGPSEAAARLEASKSFTKEICDAANAPTAGYGHFTDAEAAKAHVRANGAPIVVKADGLAAGKGVIVAMDEQ
TALDAIDDMFGGAFGGAGAEVVIEEFMEGEEASLFVLCDGEEILSIGTAQDHKRVGEGDTGLNTGGMGAYSPAPVLSAEV
EAKAMEEIVKPTMRVMAERGMPYQGVLYAGLMIKDGQPRLVEYNVRFGDPECQVLMMRLGAQALDLMQAAAEGRLADARV
NWADDHAITVVMAAAGYPGSYEKGSEIKGLDALPEDSMNMVFHAGTKADGDKILANGGRVLNVTARGESLSEARDRAYAM
VDQIDWPEGFVRRDIGWRAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=45.1990632318501, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=45.3051643192488, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=45.3051643192488, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=45.3051643192488, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=52.2352941176471, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=41.5676959619952, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=43.8914027149321, Blast_Score=327, Evalue=3e-90,
Organism=Drosophila melanogaster, GI62471619, Length=414, Percent_Identity=42.2705314009662, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24582400, Length=414, Percent_Identity=42.2705314009662, Blast_Score=298, Evalue=6e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44000; Mature: 44000

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILILGSGGREHALAWAVMQNPKCDRLIVAPGNAGIAQIADCASLNAEDGGAVVTFAEE
CEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCEEEEEECC
NAIDFVIVGPEAPLAAGVADRLRDAGILVFGPSEAAARLEASKSFTKEICDAANAPTAGY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GHFTDAEAAKAHVRANGAPIVVKADGLAAGKGVIVAMDEQTALDAIDDMFGGAFGGAGAE
CCCCCCHHHHHEEECCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCHH
VVIEEFMEGEEASLFVLCDGEEILSIGTAQDHKRVGEGDTGLNTGGMGAYSPAPVLSAEV
HHHHHHHCCCCCEEEEEECCHHHEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHH
EAKAMEEIVKPTMRVMAERGMPYQGVLYAGLMIKDGQPRLVEYNVRFGDPECQVLMMRLG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHC
AQALDLMQAAAEGRLADARVNWADDHAITVVMAAAGYPGSYEKGSEIKGLDALPEDSMNM
HHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
VFHAGTKADGDKILANGGRVLNVTARGESLSEARDRAYAMVDQIDWPEGFVRRDIGWRAL
EEEECCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MNILILGSGGREHALAWAVMQNPKCDRLIVAPGNAGIAQIADCASLNAEDGGAVVTFAEE
CEEEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCEEEEEECC
NAIDFVIVGPEAPLAAGVADRLRDAGILVFGPSEAAARLEASKSFTKEICDAANAPTAGY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GHFTDAEAAKAHVRANGAPIVVKADGLAAGKGVIVAMDEQTALDAIDDMFGGAFGGAGAE
CCCCCCHHHHHEEECCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCHH
VVIEEFMEGEEASLFVLCDGEEILSIGTAQDHKRVGEGDTGLNTGGMGAYSPAPVLSAEV
HHHHHHHCCCCCEEEEEECCHHHEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHH
EAKAMEEIVKPTMRVMAERGMPYQGVLYAGLMIKDGQPRLVEYNVRFGDPECQVLMMRLG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHC
AQALDLMQAAAEGRLADARVNWADDHAITVVMAAAGYPGSYEKGSEIKGLDALPEDSMNM
HHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
VFHAGTKADGDKILANGGRVLNVTARGESLSEARDRAYAMVDQIDWPEGFVRRDIGWRAL
EEEECCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]