Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 99081679

GI number: 99081679

Start: 1938162

End: 1939529

Strand: Direct

Name: puuA [H]

Synonym: TM1040_1838

Alternate gene names: 99081679

Gene position: 1938162-1939529 (Clockwise)

Preceding gene: 99081678

Following gene: 99081680

Centisome position: 60.55

GC content: 61.7

Gene sequence:

>1368_bases
ATGCCGACCCCGACGCGCTGGCAGGAAAAACTGCCCGAAGCTGCCACGACCTATCTGGAGGGCCGTCGTCTCGACGAGGT
TGAATGCGTTATCTCGGACTTGCCGGGCATCGCCCGGGGCAAGGCGGTGCCAGCGTCGAAATTTGCCAAGCAGGACTATT
TTTACCTGCCCGACAGCATCTTTTATCAGACCATCACCGGGGATTGGGCCGAAGCCGCCGACGAGGACGGCTGGATCGAA
AAGGACATGCTGCTGCGCCCGGACATGAGCACCGCAACCGCCGCCCCCTGGACCGGCGACTGGACTCTGCAGGTCATCCA
CGATGCCTATGACCGCGACGGCAACCCGATCCCCTTCAGCCCGCGCAACGTGCTGAAACATGTGGTGAGCCTCTATGAGG
CGCAGGGCTGGAAACCCGTGGTGGCGCCGGAGATGGAATTCTATCTCGTGGCCCGCAACGTCGACCCCGCGCGCGACATC
CAGCCCATGATGGGCCGCTCTGGCCGTCCGGCGGCGGCGCGTCAGGCCTATTCGATGACAGCGGTGGACGAATTTGGCCC
CGTCATCGACGACATCTATGATTTTGCCGAGGCGCAAGGCTTTGAAATCGACGGCATCACCCAGGAAGGCGGTGCCGGGC
AGCTGGAAATCAACCTGCGCCATGGCTCGCCGGTGAAGCTAGCCGATGAGGTGTTTTACTTCAAACGCCTGATCCGCGAG
GCCGCGCTGCGCCATGATTGCTTTGCCACCTTCATGGCAAAACCGATCGCTGATGAGCCGGGCTCTGCCATGCATATCCA
CCACTCGGTGATCGACATCGAGAGCGGCGAGAACATCTTTTCCGGTCCCCAAGGTGGTGAAACGGATGCGTTTTATCACT
TTATCGGCGGGCTGCAGAACCACCTTCCTGCCGGTCTCGCGGTGATGGCGCCCTATGTGAATTCCTATCGCCGCTATGTG
AAGAACCACGCCGCGCCGATCAATCTGGAATGGGCGCGCGACAACCGCACCACCGGCATTCGGGTGCCGCTCTCCAGTTC
CGCCTCGCGCCGGGTTGAAAACCGCATCGCCGGGATGGATTGCAACCCCTATCTCGGTATCGCGGTATCGCTGGCCTGCG
GCTACCTCGGCCTGATGGAAGAGCGCCGCCCCACCCGCCAGTTCAAAGGCGATGCCTATGAGGGCGAAGGTGACTTTCCG
CAGGTCATGGGTCAGGCGCTCGATCTCTTTGACGAGTCCAAGGCGCTTCACGAGGTGCTCGGCCCCGAATTCGCCCGGGT
CTACAGCACGGTGAAGCGCGCAGAATACGAAGAGTTCCTGCAAGTGATCTCGCCGTGGGAGCGTGAGCACCTGTTGCTCA
ACGTCTGA

Upstream 100 bases:

>100_bases
ACGCACAGCTGAACGCGGCCGAGGCCTCCCTCGACCGCCCCACCGACTCCACCCAAATTGCACAAGAGATTGCCGACTTC
TTCAAGAAAGTGAGGACCTG

Downstream 100 bases:

>100_bases
TCCATCAGCGCTGCGCCCGGTCAAAGGCGCAGCGCTTTAGGTATTTATCAAAAGATGAAGTCCCCGGTCGGCCTGTGCCG
CCTGCCGGGGCGAAAGACGA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MPTPTRWQEKLPEAATTYLEGRRLDEVECVISDLPGIARGKAVPASKFAKQDYFYLPDSIFYQTITGDWAEAADEDGWIE
KDMLLRPDMSTATAAPWTGDWTLQVIHDAYDRDGNPIPFSPRNVLKHVVSLYEAQGWKPVVAPEMEFYLVARNVDPARDI
QPMMGRSGRPAAARQAYSMTAVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLRHGSPVKLADEVFYFKRLIRE
AALRHDCFATFMAKPIADEPGSAMHIHHSVIDIESGENIFSGPQGGETDAFYHFIGGLQNHLPAGLAVMAPYVNSYRRYV
KNHAAPINLEWARDNRTTGIRVPLSSSASRRVENRIAGMDCNPYLGIAVSLACGYLGLMEERRPTRQFKGDAYEGEGDFP
QVMGQALDLFDESKALHEVLGPEFARVYSTVKRAEYEEFLQVISPWEREHLLLNV

Sequences:

>Translated_455_residues
MPTPTRWQEKLPEAATTYLEGRRLDEVECVISDLPGIARGKAVPASKFAKQDYFYLPDSIFYQTITGDWAEAADEDGWIE
KDMLLRPDMSTATAAPWTGDWTLQVIHDAYDRDGNPIPFSPRNVLKHVVSLYEAQGWKPVVAPEMEFYLVARNVDPARDI
QPMMGRSGRPAAARQAYSMTAVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLRHGSPVKLADEVFYFKRLIRE
AALRHDCFATFMAKPIADEPGSAMHIHHSVIDIESGENIFSGPQGGETDAFYHFIGGLQNHLPAGLAVMAPYVNSYRRYV
KNHAAPINLEWARDNRTTGIRVPLSSSASRRVENRIAGMDCNPYLGIAVSLACGYLGLMEERRPTRQFKGDAYEGEGDFP
QVMGQALDLFDESKALHEVLGPEFARVYSTVKRAEYEEFLQVISPWEREHLLLNV
>Mature_454_residues
PTPTRWQEKLPEAATTYLEGRRLDEVECVISDLPGIARGKAVPASKFAKQDYFYLPDSIFYQTITGDWAEAADEDGWIEK
DMLLRPDMSTATAAPWTGDWTLQVIHDAYDRDGNPIPFSPRNVLKHVVSLYEAQGWKPVVAPEMEFYLVARNVDPARDIQ
PMMGRSGRPAAARQAYSMTAVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLRHGSPVKLADEVFYFKRLIREA
ALRHDCFATFMAKPIADEPGSAMHIHHSVIDIESGENIFSGPQGGETDAFYHFIGGLQNHLPAGLAVMAPYVNSYRRYVK
NHAAPINLEWARDNRTTGIRVPLSSSASRRVENRIAGMDCNPYLGIAVSLACGYLGLMEERRPTRQFKGDAYEGEGDFPQ
VMGQALDLFDESKALHEVLGPEFARVYSTVKRAEYEEFLQVISPWEREHLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=431, Percent_Identity=23.4338747099768, Blast_Score=109, Evalue=7e-24,
Organism=Escherichia coli, GI87081870, Length=436, Percent_Identity=35.3211009174312, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1790301, Length=478, Percent_Identity=28.4518828451883, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50942; Mature: 50811

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPTRWQEKLPEAATTYLEGRRLDEVECVISDLPGIARGKAVPASKFAKQDYFYLPDSI
CCCCCCHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEECCHHH
FYQTITGDWAEAADEDGWIEKDMLLRPDMSTATAAPWTGDWTLQVIHDAYDRDGNPIPFS
HHHHHCCCHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
PRNVLKHVVSLYEAQGWKPVVAPEMEFYLVARNVDPARDIQPMMGRSGRPAAARQAYSMT
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHH
AVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLRHGSPVKLADEVFYFKRLIRE
HHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
AALRHDCFATFMAKPIADEPGSAMHIHHSVIDIESGENIFSGPQGGETDAFYHFIGGLQN
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
HLPAGLAVMAPYVNSYRRYVKNHAAPINLEWARDNRTTGIRVPLSSSASRRVENRIAGMD
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCC
CNPYLGIAVSLACGYLGLMEERRPTRQFKGDAYEGEGDFPQVMGQALDLFDESKALHEVL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GPEFARVYSTVKRAEYEEFLQVISPWEREHLLLNV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure 
PTPTRWQEKLPEAATTYLEGRRLDEVECVISDLPGIARGKAVPASKFAKQDYFYLPDSI
CCCCCHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEECCHHH
FYQTITGDWAEAADEDGWIEKDMLLRPDMSTATAAPWTGDWTLQVIHDAYDRDGNPIPFS
HHHHHCCCHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
PRNVLKHVVSLYEAQGWKPVVAPEMEFYLVARNVDPARDIQPMMGRSGRPAAARQAYSMT
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHH
AVDEFGPVIDDIYDFAEAQGFEIDGITQEGGAGQLEINLRHGSPVKLADEVFYFKRLIRE
HHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHH
AALRHDCFATFMAKPIADEPGSAMHIHHSVIDIESGENIFSGPQGGETDAFYHFIGGLQN
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
HLPAGLAVMAPYVNSYRRYVKNHAAPINLEWARDNRTTGIRVPLSSSASRRVENRIAGMD
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHCCCC
CNPYLGIAVSLACGYLGLMEERRPTRQFKGDAYEGEGDFPQVMGQALDLFDESKALHEVL
CCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GPEFARVYSTVKRAEYEEFLQVISPWEREHLLLNV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]