Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 99081680

GI number: 99081680

Start: 1939640

End: 1940950

Strand: Direct

Name: puuB [H]

Synonym: TM1040_1839

Alternate gene names: 99081680

Gene position: 1939640-1940950 (Clockwise)

Preceding gene: 99081679

Following gene: 99081681

Centisome position: 60.6

GC content: 64.38

Gene sequence:

>1311_bases
ATGACGCTGAACCTGCTGCATGCAAACGACCGACCCGGAAAATACCCCCAAAGCTGGTACGCCGCCACCGCAACGCCGGG
GGTGCATTACCCGTCTTTGACGGGTGGGGTCAGGGCGGATGTCTGCATTGTGGGCGCGGGCTACACCGGGCTCTCGGCGG
CGCTGCATCTGGCCGAGGCGGGCCGCTCCGTAGTGCTGTTGGATGCGCAGCGTGTGGGCTTTGGGGCGTCCGGGCGCAAT
GGTGGGCAGCTCGGGTCCGGCCAGCGCCAGGACCAGGAGGTGCTGGAGAAAATGGTGGGCGCCGAGGATGCCGCCCACCT
GTGGCGCCTCGGCGAAGAGGCCAAGGACCTCGTCAAAGACCTGATCTCCAAGCACCAGATCGACTGCCACCTGAAGCCGG
GCGTGGCCTGGACTGCGGAACGTTCGGGCGATGTGACGCATCTTCATGACTACGGTCGCCACCTTGAAGACCGCTATGGC
TATGATCAGATTGAGTTTCTGGATCAGGACGCCTGCCACGCCATGTGCCCCTCCCCGGCCTACAAGGGCGGTATTCTGGA
TCATGGCGCGGGGCATCTGCACCCGTTGAATTTTGCGCTGGGTCTGGCGCGGGCCGCTGCAGAGGCCGGTGCGCAGATCT
ATGAGCGCAGCGAAGTCTTTGCCATCGAGGACGGACCGCAGGTGGAAGTGCGCACGGGCGAGGGCACCGTGCGGGCCGAT
CACGTTATACTGGCCTGCAACGGCTATCTCGGCGGGCTCAACGAGCAGGTCGCCACACGCGTGATGCCGATCAACAATTT
TGTCGCCGCCACCGAGCCTCTGGGCGATGACGCCGCGCAGGTTCTGGCGCGCGATGTGGCCGTGGCGGACAGCAAATTCG
TGGTGAACTATTTTCGCCTGAGCCATGACAAGCGGCTGCTGTTTGGCGGCGGAGAAAGCTATGGTTATCGCTTCCCGAGC
GACATTGCCGCCAAGGTGCGCAAACCCATGACGGAGATCTTTCCGCATCTTCATGATGTAAAGATCGACTACGCCTGGGG
CGGCACCCTGGGGATCACCATGAAGCGTCTGCCCTATCTGGCGCGGGTGTCGCCGCGGATCCTCTCGGCCTCAGGGTATA
GCGGTCATGGGGTCGGCACCGCCACCCATGCGGGGCGGCTGCTGGCAGAGGCCATCGCAGGCCAGAGCGAGGGGTTCAAC
ACGATGGCGCGCATTCCCACGACGCCCTTCCCGGGTGGCCCCAGGATGCGCTCTCCGCTTCTGGTGCTGGCGATGACGTG
GTTCTCCTTGCGCGACCGGTTGGGGATCTGA

Upstream 100 bases:

>100_bases
CTGCGCCCGGTCAAAGGCGCAGCGCTTTAGGTATTTATCAAAAGATGAAGTCCCCGGTCGGCCTGTGCCGCCTGCCGGGG
CGAAAGACGAGGCCGACCCG

Downstream 100 bases:

>100_bases
TCCGGCCTGACGTCGGGGGCCTTGCCCGCAGTTTGAGATGCCCGGGGGCTCTGCCCACAGTCTAAAAGTTCGGGGGCTCT
GCCCAAGGTTGTTGTCTGTT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTLNLLHANDRPGKYPQSWYAATATPGVHYPSLTGGVRADVCIVGAGYTGLSAALHLAEAGRSVVLLDAQRVGFGASGRN
GGQLGSGQRQDQEVLEKMVGAEDAAHLWRLGEEAKDLVKDLISKHQIDCHLKPGVAWTAERSGDVTHLHDYGRHLEDRYG
YDQIEFLDQDACHAMCPSPAYKGGILDHGAGHLHPLNFALGLARAAAEAGAQIYERSEVFAIEDGPQVEVRTGEGTVRAD
HVILACNGYLGGLNEQVATRVMPINNFVAATEPLGDDAAQVLARDVAVADSKFVVNYFRLSHDKRLLFGGGESYGYRFPS
DIAAKVRKPMTEIFPHLHDVKIDYAWGGTLGITMKRLPYLARVSPRILSASGYSGHGVGTATHAGRLLAEAIAGQSEGFN
TMARIPTTPFPGGPRMRSPLLVLAMTWFSLRDRLGI

Sequences:

>Translated_436_residues
MTLNLLHANDRPGKYPQSWYAATATPGVHYPSLTGGVRADVCIVGAGYTGLSAALHLAEAGRSVVLLDAQRVGFGASGRN
GGQLGSGQRQDQEVLEKMVGAEDAAHLWRLGEEAKDLVKDLISKHQIDCHLKPGVAWTAERSGDVTHLHDYGRHLEDRYG
YDQIEFLDQDACHAMCPSPAYKGGILDHGAGHLHPLNFALGLARAAAEAGAQIYERSEVFAIEDGPQVEVRTGEGTVRAD
HVILACNGYLGGLNEQVATRVMPINNFVAATEPLGDDAAQVLARDVAVADSKFVVNYFRLSHDKRLLFGGGESYGYRFPS
DIAAKVRKPMTEIFPHLHDVKIDYAWGGTLGITMKRLPYLARVSPRILSASGYSGHGVGTATHAGRLLAEAIAGQSEGFN
TMARIPTTPFPGGPRMRSPLLVLAMTWFSLRDRLGI
>Mature_435_residues
TLNLLHANDRPGKYPQSWYAATATPGVHYPSLTGGVRADVCIVGAGYTGLSAALHLAEAGRSVVLLDAQRVGFGASGRNG
GQLGSGQRQDQEVLEKMVGAEDAAHLWRLGEEAKDLVKDLISKHQIDCHLKPGVAWTAERSGDVTHLHDYGRHLEDRYGY
DQIEFLDQDACHAMCPSPAYKGGILDHGAGHLHPLNFALGLARAAAEAGAQIYERSEVFAIEDGPQVEVRTGEGTVRADH
VILACNGYLGGLNEQVATRVMPINNFVAATEPLGDDAAQVLARDVAVADSKFVVNYFRLSHDKRLLFGGGESYGYRFPSD
IAAKVRKPMTEIFPHLHDVKIDYAWGGTLGITMKRLPYLARVSPRILSASGYSGHGVGTATHAGRLLAEAIAGQSEGFNT
MARIPTTPFPGGPRMRSPLLVLAMTWFSLRDRLGI

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=42.7230046948357, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46983; Mature: 46852

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNLLHANDRPGKYPQSWYAATATPGVHYPSLTGGVRADVCIVGAGYTGLSAALHLAEA
CEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHC
GRSVVLLDAQRVGFGASGRNGGQLGSGQRQDQEVLEKMVGAEDAAHLWRLGEEAKDLVKD
CCEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHH
LISKHQIDCHLKPGVAWTAERSGDVTHLHDYGRHLEDRYGYDQIEFLDQDACHAMCPSPA
HHHHCCCCEEECCCEEEECCCCCCEEHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCCCCC
YKGGILDHGAGHLHPLNFALGLARAAAEAGAQIYERSEVFAIEDGPQVEVRTGEGTVRAD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEC
HVILACNGYLGGLNEQVATRVMPINNFVAATEPLGDDAAQVLARDVAVADSKFVVNYFRL
EEEEEECCCCCCCCHHHHHHEECCCCCEECCCCCCHHHHHHHHHHHHHCCCHHHEEHHHH
SHDKRLLFGGGESYGYRFPSDIAAKVRKPMTEIFPHLHDVKIDYAWGGTLGITMKRLPYL
CCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHCCCHHHEEEEEEECCEEEHHHHHCCHH
ARVSPRILSASGYSGHGVGTATHAGRLLAEAIAGQSEGFNTMARIPTTPFPGGPRMRSPL
HHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCH
LVLAMTWFSLRDRLGI
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLNLLHANDRPGKYPQSWYAATATPGVHYPSLTGGVRADVCIVGAGYTGLSAALHLAEA
EEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHC
GRSVVLLDAQRVGFGASGRNGGQLGSGQRQDQEVLEKMVGAEDAAHLWRLGEEAKDLVKD
CCEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHH
LISKHQIDCHLKPGVAWTAERSGDVTHLHDYGRHLEDRYGYDQIEFLDQDACHAMCPSPA
HHHHCCCCEEECCCEEEECCCCCCEEHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCCCCC
YKGGILDHGAGHLHPLNFALGLARAAAEAGAQIYERSEVFAIEDGPQVEVRTGEGTVRAD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEC
HVILACNGYLGGLNEQVATRVMPINNFVAATEPLGDDAAQVLARDVAVADSKFVVNYFRL
EEEEEECCCCCCCCHHHHHHEECCCCCEECCCCCCHHHHHHHHHHHHHCCCHHHEEHHHH
SHDKRLLFGGGESYGYRFPSDIAAKVRKPMTEIFPHLHDVKIDYAWGGTLGITMKRLPYL
CCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHCCCHHHEEEEEEECCEEEHHHHHCCHH
ARVSPRILSASGYSGHGVGTATHAGRLLAEAIAGQSEGFNTMARIPTTPFPGGPRMRSPL
HHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCH
LVLAMTWFSLRDRLGI
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]