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The map label for this gene is puuA [H]

Identifier: 99081677

GI number: 99081677

Start: 1936107

End: 1937426

Strand: Direct

Name: puuA [H]

Synonym: TM1040_1836

Alternate gene names: 99081677

Gene position: 1936107-1937426 (Clockwise)

Preceding gene: 99081676

Following gene: 99081678

Centisome position: 60.49

GC content: 64.02

Gene sequence:

>1320_bases
ATGGATCTCTCTGCGCTCAAGACAATTCGCATCGCCGCCTGTGATCTCAACGGGCAGATGCGCGGCAAACGACTGCCTGC
GGGCATGGCGGCAAAGCTGGGCGAAGGCTCGGCGCGGATGCCGTTCTCGGTTCTGAACGTCGACATCTGGGGGCGTGACA
TCGAAGACAGCCCGCTGGTGTTTGAAACCGGCGATGCCGATGGCATCCTTCTGCCCACCAAACGCGGCGCGGTGCCGATG
CCGTGGCTTGCGACCCCCTCGGCGCTGGTGCCGATGAAGATGGTGCATGAAGATGGCCGCCCTTTCCTTGGCGATCCGCG
TCAGGTGCTGGCGCATGTGCTGGACCGCTACGCCGAAAAAGGCTGGCAGGTCGCCGCCGCAACCGAACTTGAGTTCATCC
TTGTCGATGACGCGGGCACCGAACCCCTGCCCCCGATCGACCCCCTCACAGGGCGCGCGCTCGATCAGCAATCGGTGCTC
TCGATTGCCGAACTGGACGCCTATGACGACTTTTTTACCGATCTCTATGACGGCGCTGAGGCAATGGGCATCCCGGCGCA
GGATGCAATTTCCGAGGCCGGTCTGGGCCAGTTCGAGATCAACCTGACCCATCAGGACGCGATGCGCGCCGCCGATGATA
CCTGGCTCTTTAAGGCGCTGGTGAAAGGCGTTGCACGCAAGCACGGCTTTGCCGCGACCTTTATGGCCAAGCCCTATGCC
GAGGAATCCGGCAATGGGATGCACGTGCATTTCTCGGTGGTGGACAAAGACGGCGTCAATATCTTTGACAATGGCGGCCC
CGAGGGCACGCCTGCCCTGCACGCCGCCGTGGCGGGCTGCATCAACGCGATGCGCGACAGCTCGCTTGTTTTTGCCCCGC
ATGGCAATTCCTACGACCGTCTGGTGCCGGGTGCCCATGCGCCGACCTCGGCGGCCTGGGCCTATGAGAACCGCACTGCG
GCCATCCGCATTCCCGGCGGCAGCCCGAAGGCGCGGCGGATTGAGCACCGGGTTTCCGGCGGCGACATCAACCCCTATTT
GATGCTGGCCACCGTGCTGGGCGCAGCCCTCATCGGCATGGAGGATGGCGCCACGCCGCCGCCGCCCTCTGAGGGCAATA
TCTATGACATCGACGGCCTGCCGCAGCTTGCGGGCAGCTGGGCCGAGGCGATCGACCTCTTTGAAAACAGCCCCCTCATC
GCCCGCATCCTGCCGCCGATGGCGATCCGCAATCTGGTGATGACAAAGCGCCAAGAGCTCAAGGGCTTTGCCGAGCGCCC
CCCCGAGAGCCATTGGCTCTCGTGGCTGGAGGCCGTATGA

Upstream 100 bases:

>100_bases
TTGCGCAGGGCGCAGTCTTGCGAATAATTTTTGATCAAATTCTCTTGCCAGCGCCCGCTGCTCACGCCAATGTGTCTCCA
GCAAGACAAGGAGCACGCGC

Downstream 100 bases:

>100_bases
CGCGGGGAGCGCCCCTTGCGTCTCACGTCCATGTTTTGATCATATTTGGTGACTTATGAAGATCGGCATCCTGCAAACCG
GCCACGCGCCCGACGAACTG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MDLSALKTIRIAACDLNGQMRGKRLPAGMAAKLGEGSARMPFSVLNVDIWGRDIEDSPLVFETGDADGILLPTKRGAVPM
PWLATPSALVPMKMVHEDGRPFLGDPRQVLAHVLDRYAEKGWQVAAATELEFILVDDAGTEPLPPIDPLTGRALDQQSVL
SIAELDAYDDFFTDLYDGAEAMGIPAQDAISEAGLGQFEINLTHQDAMRAADDTWLFKALVKGVARKHGFAATFMAKPYA
EESGNGMHVHFSVVDKDGVNIFDNGGPEGTPALHAAVAGCINAMRDSSLVFAPHGNSYDRLVPGAHAPTSAAWAYENRTA
AIRIPGGSPKARRIEHRVSGGDINPYLMLATVLGAALIGMEDGATPPPPSEGNIYDIDGLPQLAGSWAEAIDLFENSPLI
ARILPPMAIRNLVMTKRQELKGFAERPPESHWLSWLEAV

Sequences:

>Translated_439_residues
MDLSALKTIRIAACDLNGQMRGKRLPAGMAAKLGEGSARMPFSVLNVDIWGRDIEDSPLVFETGDADGILLPTKRGAVPM
PWLATPSALVPMKMVHEDGRPFLGDPRQVLAHVLDRYAEKGWQVAAATELEFILVDDAGTEPLPPIDPLTGRALDQQSVL
SIAELDAYDDFFTDLYDGAEAMGIPAQDAISEAGLGQFEINLTHQDAMRAADDTWLFKALVKGVARKHGFAATFMAKPYA
EESGNGMHVHFSVVDKDGVNIFDNGGPEGTPALHAAVAGCINAMRDSSLVFAPHGNSYDRLVPGAHAPTSAAWAYENRTA
AIRIPGGSPKARRIEHRVSGGDINPYLMLATVLGAALIGMEDGATPPPPSEGNIYDIDGLPQLAGSWAEAIDLFENSPLI
ARILPPMAIRNLVMTKRQELKGFAERPPESHWLSWLEAV
>Mature_439_residues
MDLSALKTIRIAACDLNGQMRGKRLPAGMAAKLGEGSARMPFSVLNVDIWGRDIEDSPLVFETGDADGILLPTKRGAVPM
PWLATPSALVPMKMVHEDGRPFLGDPRQVLAHVLDRYAEKGWQVAAATELEFILVDDAGTEPLPPIDPLTGRALDQQSVL
SIAELDAYDDFFTDLYDGAEAMGIPAQDAISEAGLGQFEINLTHQDAMRAADDTWLFKALVKGVARKHGFAATFMAKPYA
EESGNGMHVHFSVVDKDGVNIFDNGGPEGTPALHAAVAGCINAMRDSSLVFAPHGNSYDRLVPGAHAPTSAAWAYENRTA
AIRIPGGSPKARRIEHRVSGGDINPYLMLATVLGAALIGMEDGATPPPPSEGNIYDIDGLPQLAGSWAEAIDLFENSPLI
ARILPPMAIRNLVMTKRQELKGFAERPPESHWLSWLEAV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=430, Percent_Identity=24.1860465116279, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87081870, Length=386, Percent_Identity=39.119170984456, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1790301, Length=364, Percent_Identity=31.043956043956, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 47185; Mature: 47185

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSALKTIRIAACDLNGQMRGKRLPAGMAAKLGEGSARMPFSVLNVDIWGRDIEDSPLV
CCHHHHCEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCCCCEE
FETGDADGILLPTKRGAVPMPWLATPSALVPMKMVHEDGRPFLGDPRQVLAHVLDRYAEK
EECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
GWQVAAATELEFILVDDAGTEPLPPIDPLTGRALDQQSVLSIAELDAYDDFFTDLYDGAE
CCEEEEECEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
AMGIPAQDAISEAGLGQFEINLTHQDAMRAADDTWLFKALVKGVARKHGFAATFMAKPYA
HCCCCHHHHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCC
EESGNGMHVHFSVVDKDGVNIFDNGGPEGTPALHAAVAGCINAMRDSSLVFAPHGNSYDR
CCCCCEEEEEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
LVPGAHAPTSAAWAYENRTAAIRIPGGSPKARRIEHRVSGGDINPYLMLATVLGAALIGM
CCCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
EDGATPPPPSEGNIYDIDGLPQLAGSWAEAIDLFENSPLIARILPPMAIRNLVMTKRQEL
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHH
KGFAERPPESHWLSWLEAV
HHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MDLSALKTIRIAACDLNGQMRGKRLPAGMAAKLGEGSARMPFSVLNVDIWGRDIEDSPLV
CCHHHHCEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEEEEECCCCCCCCEE
FETGDADGILLPTKRGAVPMPWLATPSALVPMKMVHEDGRPFLGDPRQVLAHVLDRYAEK
EECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
GWQVAAATELEFILVDDAGTEPLPPIDPLTGRALDQQSVLSIAELDAYDDFFTDLYDGAE
CCEEEEECEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
AMGIPAQDAISEAGLGQFEINLTHQDAMRAADDTWLFKALVKGVARKHGFAATFMAKPYA
HCCCCHHHHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCCC
EESGNGMHVHFSVVDKDGVNIFDNGGPEGTPALHAAVAGCINAMRDSSLVFAPHGNSYDR
CCCCCEEEEEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
LVPGAHAPTSAAWAYENRTAAIRIPGGSPKARRIEHRVSGGDINPYLMLATVLGAALIGM
CCCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
EDGATPPPPSEGNIYDIDGLPQLAGSWAEAIDLFENSPLIARILPPMAIRNLVMTKRQEL
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHH
KGFAERPPESHWLSWLEAV
HHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]