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The map label for this gene is rfbD [H]

Identifier: 99081323

GI number: 99081323

Start: 1583603

End: 1584724

Strand: Reverse

Name: rfbD [H]

Synonym: TM1040_1482

Alternate gene names: 99081323

Gene position: 1584724-1583603 (Counterclockwise)

Preceding gene: 99081324

Following gene: 99081322

Centisome position: 49.51

GC content: 58.2

Gene sequence:

>1122_bases
ATGACCAAACGTGCGCTCATCACCGGGGTCACCGGTCAAGACGGCTCTTACCTGGCAGAGTTCCTGCTGGAGAAGGGCTA
TGAGGTGCATGGCATTAAGCGCCGCGCCTCATCCTTCAACACCAGCCGTATCGACCATATCTATCAGGATCCGCACACGG
ATCGGGCGCGCTTCAAGCTGCATTATGGAGATCTGGCCGACACTTCCAACCTGACGCGGATCTTGCGCGAGGTCGAACCG
GATGAGGTGTATAACCTCGGTGCGCAATCTCATGTCGCGGTGTCTTTTGAGGCTCCGGAATACACGGCAGATGTGGATGC
CATTGGCACACTGCGCCTCTTGGAGGCGATCCGTTTCCTCGGTCTTGAGCAGAAGACCCGGTTTTATCAGGCCTCGACAT
CTGAGCTCTATGGTCTGGTGCAGGAGACGCCGCAACGCGAAACGACTCCGTTTCACCCCCGCTCGCCCTATGCGGTGGCC
AAGATGTACGCCTATTGGATCACGGTGAACTACCGCGAGGCTTATGGCCTTTACGCTTGTAACGGCATTCTGTTCAACCA
TGAGAGCCCGCGCCGGGGTGAGACATTTGTGACCCGCAAGATCACCCGCGGCCTTGCCAATATCGCACAGGGGCTCGAGC
CCTGCCTTTACATGGGCAATATCGACAGCCTGCGGGATTGGGGTCATGCCAAGGACTATGTGCGCATGCAGTGGATGATG
CTGCAGCAAGACACGCCCGAGGATTTCGTGATCGCCACGGGCAAGCAATCTTCCGTGCGGGAGTTCATCATCTGGTCGGC
AGCGGAACTCGGGATTGAGCTCGAGTTTTCCGGCAAGGGCGTGGACGAGGTGGCAACTGTCACCGCGATCAACGGTGATG
CCGCACCCGCGCTCAGGGTGGGCGATATTGTCATGCGTATTGATCCGCGGTACTTCCGTCCGGCCGAGGTCGACACGCTC
CTTGGAGATCCGACAAAGGCGAAGGAAAAACTCGGCTGGGTGCCGGAGATCACGGCGCAGGAGATGTGCGCGGAAATGAT
TGAGGAAGACCTGAAAACCGCGCGCCGTCATGCCCTTTTGAAAGAACATGGCTATGATCTGCCGGTCTGCATCGGGGAGT
AA

Upstream 100 bases:

>100_bases
CTGCCCAATGGACTCAAACCTGAGACAAAGCGCCTGAACCCGCGCCTGAACCTTGAGCCTGCACGCCCCAAAAGCGCGTG
CCCGAATACACGAGGATAAC

Downstream 100 bases:

>100_bases
ACCCTGCCATGACATCTGAACAGGTGTTTGATCTACGGGGAAAGCGCATTTTTGTGGCTGGTCATCGTGGAATGGTCGGC
GGTGCGGTCTTGCGACGGCT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTKRALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDPHTDRARFKLHYGDLADTSNLTRILREVEP
DEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFLGLEQKTRFYQASTSELYGLVQETPQRETTPFHPRSPYAVA
KMYAYWITVNYREAYGLYACNGILFNHESPRRGETFVTRKITRGLANIAQGLEPCLYMGNIDSLRDWGHAKDYVRMQWMM
LQQDTPEDFVIATGKQSSVREFIIWSAAELGIELEFSGKGVDEVATVTAINGDAAPALRVGDIVMRIDPRYFRPAEVDTL
LGDPTKAKEKLGWVPEITAQEMCAEMIEEDLKTARRHALLKEHGYDLPVCIGE

Sequences:

>Translated_373_residues
MTKRALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDPHTDRARFKLHYGDLADTSNLTRILREVEP
DEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFLGLEQKTRFYQASTSELYGLVQETPQRETTPFHPRSPYAVA
KMYAYWITVNYREAYGLYACNGILFNHESPRRGETFVTRKITRGLANIAQGLEPCLYMGNIDSLRDWGHAKDYVRMQWMM
LQQDTPEDFVIATGKQSSVREFIIWSAAELGIELEFSGKGVDEVATVTAINGDAAPALRVGDIVMRIDPRYFRPAEVDTL
LGDPTKAKEKLGWVPEITAQEMCAEMIEEDLKTARRHALLKEHGYDLPVCIGE
>Mature_372_residues
TKRALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDPHTDRARFKLHYGDLADTSNLTRILREVEPD
EVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFLGLEQKTRFYQASTSELYGLVQETPQRETTPFHPRSPYAVAK
MYAYWITVNYREAYGLYACNGILFNHESPRRGETFVTRKITRGLANIAQGLEPCLYMGNIDSLRDWGHAKDYVRMQWMML
QQDTPEDFVIATGKQSSVREFIIWSAAELGIELEFSGKGVDEVATVTAINGDAAPALRVGDIVMRIDPRYFRPAEVDTLL
GDPTKAKEKLGWVPEITAQEMCAEMIEEDLKTARRHALLKEHGYDLPVCIGE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=352, Percent_Identity=58.8068181818182, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1788366, Length=371, Percent_Identity=78.167115902965, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=27.1428571428571, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788353, Length=257, Percent_Identity=26.0700389105058, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=351, Percent_Identity=61.8233618233618, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17539424, Length=351, Percent_Identity=60.3988603988604, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901788, Length=351, Percent_Identity=60.3988603988604, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901790, Length=351, Percent_Identity=60.3988603988604, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901786, Length=351, Percent_Identity=60.3988603988604, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17539422, Length=351, Percent_Identity=60.3988603988604, Blast_Score=446, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24158427, Length=358, Percent_Identity=58.9385474860335, Blast_Score=446, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 42198; Mature: 42067

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDPHTDRARFKL
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCCCCCEEEEE
HYGDLADTSNLTRILREVEPDEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFL
EECCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
GLEQKTRFYQASTSELYGLVQETPQRETTPFHPRSPYAVAKMYAYWITVNYREAYGLYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHCCEEEE
NGILFNHESPRRGETFVTRKITRGLANIAQGLEPCLYMGNIDSLRDWGHAKDYVRMQWMM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCHHHHHHHHHHH
LQQDTPEDFVIATGKQSSVREFIIWSAAELGIELEFSGKGVDEVATVTAINGDAAPALRV
CCCCCCCCEEEECCCCHHHHHHHEEEHHHHCEEEEECCCCCCCEEEEEEECCCCCCCEEE
GDIVMRIDPRYFRPAEVDTLLGDPTKAKEKLGWVPEITAQEMCAEMIEEDLKTARRHALL
CCEEEEECCCCCCCCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KEHGYDLPVCIGE
HHCCCCCCEEECC
>Mature Secondary Structure 
TKRALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTSRIDHIYQDPHTDRARFKL
CCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCCCCCEEEEE
HYGDLADTSNLTRILREVEPDEVYNLGAQSHVAVSFEAPEYTADVDAIGTLRLLEAIRFL
EECCCCCHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
GLEQKTRFYQASTSELYGLVQETPQRETTPFHPRSPYAVAKMYAYWITVNYREAYGLYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHCCEEEE
NGILFNHESPRRGETFVTRKITRGLANIAQGLEPCLYMGNIDSLRDWGHAKDYVRMQWMM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCHHHHHHHHHHH
LQQDTPEDFVIATGKQSSVREFIIWSAAELGIELEFSGKGVDEVATVTAINGDAAPALRV
CCCCCCCCEEEECCCCHHHHHHHEEEHHHHCEEEEECCCCCCCEEEEEEECCCCCCCEEE
GDIVMRIDPRYFRPAEVDTLLGDPTKAKEKLGWVPEITAQEMCAEMIEEDLKTARRHALL
CCEEEEECCCCCCCCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KEHGYDLPVCIGE
HHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]