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The map label for this gene is fcl [C]

Identifier: 99081322

GI number: 99081322

Start: 1582635

End: 1583594

Strand: Reverse

Name: fcl [C]

Synonym: TM1040_1481

Alternate gene names: 99081322

Gene position: 1583594-1582635 (Counterclockwise)

Preceding gene: 99081323

Following gene: 99081320

Centisome position: 49.47

GC content: 59.06

Gene sequence:

>960_bases
ATGACATCTGAACAGGTGTTTGATCTACGGGGAAAGCGCATTTTTGTGGCTGGTCATCGTGGAATGGTCGGCGGTGCGGT
CTTGCGACGGCTGGCCGAAGAAGACTGTGAGGTCGTGACTGCCGGCCGCGAGGATCTGGATCTTACGCGGCAACAGGCAG
TGATGGAGTGGATGGCCGCAACCCGTCCGGATGCCATCATCATGGCTGCAGCACGGGTCGGGGGTATCAAGGCGAACAGC
GACTATCCCGTTGATTTCCTGTTGCAAAACCTTCAGATCGAAACCAATCTTGCCGAGGCTGCACATGCTGCAGATGTCCA
GCGTTTCCTGTTTCTGGGCTCTTCCTGCATCTATCCGAAATTCGCCCCGCAACCCATTCCCGAGGCAAGCCTGCTGACTG
GCGCGCTCGAGCCGAGCAACGAATGGTACGCGGTGGCCAAGATCGCAGGCATCAAGCTGATGCAGGCCTATCGCCAGCAA
TACGGGCGGGATTGGATTTCGGCGATGCCCACCAATCTCTATGGGCCGGGGGACAACTATGATCTTGAGACCAGTCATGT
GCTGCCCGCTCTGTTGCACAAGTTCCATACTGCTCGTTTGACGGGGGCGGATCAGGTCACGCTCTGGGGGTCGGGCACTC
CGCTGCGCGAGTTTCTTCATTGCGACGATCTGGCCGATGCGCTGGTGTTCTTGCTGAAACACTATTCGGGTGCGGACCAT
GTCAATGTTGGCTCGGGCAAGGAAATCAGCATTCGCGCGCTCGCCGAACTCATTGCCGAGATCGTGGGTGTGAGCCCCGA
GTTGGTTTTTGACAGCTCAAAGCCAGATGGAACGCCGCGCAAGCTGATGGACAGCGCGCGTTTGGCGGCCATGGGCTGGT
CTGGCGCACGCCCCCTGCGCGACGGGATCGCAGAAACCTACGCGGCGTTTGTGGCGCAACTGGACAGCGTTGAAGCCTGA

Upstream 100 bases:

>100_bases
CGGAAATGATTGAGGAAGACCTGAAAACCGCGCGCCGTCATGCCCTTTTGAAAGAACATGGCTATGATCTGCCGGTCTGC
ATCGGGGAGTAAACCCTGCC

Downstream 100 bases:

>100_bases
ACTTTGATACTCTTGGCTGCGCGACACTGACCATGAGGCCGCGCGCAGCCCAGTTCCTCGAGGATAATTGGGATCCCCCG
CATCGCTGTCAGCCAGGATG

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTSEQVFDLRGKRIFVAGHRGMVGGAVLRRLAEEDCEVVTAGREDLDLTRQQAVMEWMAATRPDAIIMAAARVGGIKANS
DYPVDFLLQNLQIETNLAEAAHAADVQRFLFLGSSCIYPKFAPQPIPEASLLTGALEPSNEWYAVAKIAGIKLMQAYRQQ
YGRDWISAMPTNLYGPGDNYDLETSHVLPALLHKFHTARLTGADQVTLWGSGTPLREFLHCDDLADALVFLLKHYSGADH
VNVGSGKEISIRALAELIAEIVGVSPELVFDSSKPDGTPRKLMDSARLAAMGWSGARPLRDGIAETYAAFVAQLDSVEA

Sequences:

>Translated_319_residues
MTSEQVFDLRGKRIFVAGHRGMVGGAVLRRLAEEDCEVVTAGREDLDLTRQQAVMEWMAATRPDAIIMAAARVGGIKANS
DYPVDFLLQNLQIETNLAEAAHAADVQRFLFLGSSCIYPKFAPQPIPEASLLTGALEPSNEWYAVAKIAGIKLMQAYRQQ
YGRDWISAMPTNLYGPGDNYDLETSHVLPALLHKFHTARLTGADQVTLWGSGTPLREFLHCDDLADALVFLLKHYSGADH
VNVGSGKEISIRALAELIAEIVGVSPELVFDSSKPDGTPRKLMDSARLAAMGWSGARPLRDGIAETYAAFVAQLDSVEA
>Mature_318_residues
TSEQVFDLRGKRIFVAGHRGMVGGAVLRRLAEEDCEVVTAGREDLDLTRQQAVMEWMAATRPDAIIMAAARVGGIKANSD
YPVDFLLQNLQIETNLAEAAHAADVQRFLFLGSSCIYPKFAPQPIPEASLLTGALEPSNEWYAVAKIAGIKLMQAYRQQY
GRDWISAMPTNLYGPGDNYDLETSHVLPALLHKFHTARLTGADQVTLWGSGTPLREFLHCDDLADALVFLLKHYSGADHV
NVGSGKEISIRALAELIAEIVGVSPELVFDSSKPDGTPRKLMDSARLAAMGWSGARPLRDGIAETYAAFVAQLDSVEA

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=308, Percent_Identity=32.4675324675325, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI7657641, Length=229, Percent_Identity=26.2008733624454, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=51.5923566878981, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17554428, Length=313, Percent_Identity=30.9904153354633, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19922778, Length=291, Percent_Identity=32.9896907216495, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34742; Mature: 34611

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEQVFDLRGKRIFVAGHRGMVGGAVLRRLAEEDCEVVTAGREDLDLTRQQAVMEWMAA
CCCCHHECCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
TRPDAIIMAAARVGGIKANSDYPVDFLLQNLQIETNLAEAAHAADVQRFLFLGSSCIYPK
CCCCCEEEEHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FAPQPIPEASLLTGALEPSNEWYAVAKIAGIKLMQAYRQQYGRDWISAMPTNLYGPGDNY
CCCCCCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCC
DLETSHVLPALLHKFHTARLTGADQVTLWGSGTPLREFLHCDDLADALVFLLKHYSGADH
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNVGSGKEISIRALAELIAEIVGVSPELVFDSSKPDGTPRKLMDSARLAAMGWSGARPLR
EECCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
DGIAETYAAFVAQLDSVEA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSEQVFDLRGKRIFVAGHRGMVGGAVLRRLAEEDCEVVTAGREDLDLTRQQAVMEWMAA
CCCHHECCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
TRPDAIIMAAARVGGIKANSDYPVDFLLQNLQIETNLAEAAHAADVQRFLFLGSSCIYPK
CCCCCEEEEHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FAPQPIPEASLLTGALEPSNEWYAVAKIAGIKLMQAYRQQYGRDWISAMPTNLYGPGDNY
CCCCCCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCC
DLETSHVLPALLHKFHTARLTGADQVTLWGSGTPLREFLHCDDLADALVFLLKHYSGADH
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNVGSGKEISIRALAELIAEIVGVSPELVFDSSKPDGTPRKLMDSARLAAMGWSGARPLR
EECCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHH
DGIAETYAAFVAQLDSVEA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]