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The map label for this gene is ispDF

Identifier: 99081205

GI number: 99081205

Start: 1458902

End: 1460050

Strand: Direct

Name: ispDF

Synonym: TM1040_1364

Alternate gene names: 99081205

Gene position: 1458902-1460050 (Clockwise)

Preceding gene: 99081195

Following gene: 99081206

Centisome position: 45.58

GC content: 61.53

Gene sequence:

>1149_bases
ATGACCCTCGCCGTATTGATCGTTGCCGCCGGCAAAGGCACCCGCGCAGGCGGTGGTCTCGCCAAGCAATGGCGCCCACT
GGCCGGGCGACTGGTCATCGACTGGACGATCGAGGCCTTTCAACGTGCGGGGTGCGGCACCATCATGGTTGTGCGCGATC
CCGACAATGAGCACGCGATCGAGGCGCTTGCGCCCTACCCTGAATTATTGCTCGCAGATGGCGGTCCCTCGCGGTCTGAA
TCCGTGCGTAACGGTTTGATTGCGCTCCAAGAGATCGGTGTCGAACGCGTTCTTATTCATGACGCGGCGCGTCCATGTGT
GTGTCCTCAGGTGATCCAACAGGTGCTCGACGCACTTGATGACACGCCTGCTGCCGCGCCAGGACTTGCGGTGACAGATG
CGCTTTGGACCGGGGCCGATGGCCATGTCACAGGCACGCAGGACCGAAGCGCGCTCTTTGCGGCGCAAACGCCGCAAGGC
TTTCATTTTGACGCGATCCTTGCGGCGCATATGCGCCACGACGGCACCGCAGCGGATGATGTCGAGGTTGCCCGTCAAGC
GGGGCTCGCGGTCCGTATCACGCCGGGTGACGTCAATAATATCAAGATCACCCGGCCCGAAGATTTCTCCCGCGCCGAGC
ACATATTGAGGAGCACCATGGACAACATTCCTGACATCAGGCTTGGAAATGGCTATGACGTTCACCGGTTCGGACCCGGG
GATCATGTCATGCTCTGTGGGGTTCAAGTGCCGCATGAGCGCGGTCTGCAAGGCCATTCCGATGCGGATGTGGGCATGCA
CGCGGTCACCGACGCACTCTACGGGGCGATGGCAGAGGGCGACATCGGCCGCCACTTCCCGCCAAGCGACCCTCAGTGGA
AAGGCGCGGCGTCGGACATCTTCCTGCGCCATGCGGTCGAATTGGCACGCTCCAAAGGGTTCACCATCAATAACGTGGAT
TGCACCCTCGTCTGTGAATACCCCAAAGTCGGCCCCCACGCAGAGGCGATGCGCGCCCGGATGGCAGAGATCATGGGCAT
GGATATGGGACGCCTCTCGATCAAGGCGACAACTTCAGAGCGGCTTGGGTTCACCGGTCGCAAAGAAGGCATCGCGGCAC
TGGCGACAGCAACATTGGTGCGGGCATGA

Upstream 100 bases:

>100_bases
TGCCTATTTTTTAATCACACCTGCTCGAAACATTGCTACTTTATCCGCCGCAGCTTAAACACGCCGGCAAGTTGCTCATG
CACAGGACGAACACCGCGCC

Downstream 100 bases:

>100_bases
CACTTCCACAACTTCTCGGAACCTGTTGCGGAATAGGGTATTTTCGCCCTGCGCCCGGAACCTGGGGGTCACTGGCCGCG
CTGCCGTTGACCTATGTGTT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTLAVLIVAAGKGTRAGGGLAKQWRPLAGRLVIDWTIEAFQRAGCGTIMVVRDPDNEHAIEALAPYPELLLADGGPSRSE
SVRNGLIALQEIGVERVLIHDAARPCVCPQVIQQVLDALDDTPAAAPGLAVTDALWTGADGHVTGTQDRSALFAAQTPQG
FHFDAILAAHMRHDGTAADDVEVARQAGLAVRITPGDVNNIKITRPEDFSRAEHILRSTMDNIPDIRLGNGYDVHRFGPG
DHVMLCGVQVPHERGLQGHSDADVGMHAVTDALYGAMAEGDIGRHFPPSDPQWKGAASDIFLRHAVELARSKGFTINNVD
CTLVCEYPKVGPHAEAMRARMAEIMGMDMGRLSIKATTSERLGFTGRKEGIAALATATLVRA

Sequences:

>Translated_382_residues
MTLAVLIVAAGKGTRAGGGLAKQWRPLAGRLVIDWTIEAFQRAGCGTIMVVRDPDNEHAIEALAPYPELLLADGGPSRSE
SVRNGLIALQEIGVERVLIHDAARPCVCPQVIQQVLDALDDTPAAAPGLAVTDALWTGADGHVTGTQDRSALFAAQTPQG
FHFDAILAAHMRHDGTAADDVEVARQAGLAVRITPGDVNNIKITRPEDFSRAEHILRSTMDNIPDIRLGNGYDVHRFGPG
DHVMLCGVQVPHERGLQGHSDADVGMHAVTDALYGAMAEGDIGRHFPPSDPQWKGAASDIFLRHAVELARSKGFTINNVD
CTLVCEYPKVGPHAEAMRARMAEIMGMDMGRLSIKATTSERLGFTGRKEGIAALATATLVRA
>Mature_381_residues
TLAVLIVAAGKGTRAGGGLAKQWRPLAGRLVIDWTIEAFQRAGCGTIMVVRDPDNEHAIEALAPYPELLLADGGPSRSES
VRNGLIALQEIGVERVLIHDAARPCVCPQVIQQVLDALDDTPAAAPGLAVTDALWTGADGHVTGTQDRSALFAAQTPQGF
HFDAILAAHMRHDGTAADDVEVARQAGLAVRITPGDVNNIKITRPEDFSRAEHILRSTMDNIPDIRLGNGYDVHRFGPGD
HVMLCGVQVPHERGLQGHSDADVGMHAVTDALYGAMAEGDIGRHFPPSDPQWKGAASDIFLRHAVELARSKGFTINNVDC
TLVCEYPKVGPHAEAMRARMAEIMGMDMGRLSIKATTSERLGFTGRKEGIAALATATLVRA

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=50.3184713375796, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=29.2576419213974, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_SILST (Q1GGW9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613359.1
- ProteinModelPortal:   Q1GGW9
- SMR:   Q1GGW9
- STRING:   Q1GGW9
- GeneID:   4076381
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1364
- NMPDR:   fig|292414.1.peg.3529
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q1GGW9
- ProtClustDB:   CLSK933757
- BioCyc:   SSP292414:TM1040_1364-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40754; Mature: 40623

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAVLIVAAGKGTRAGGGLAKQWRPLAGRLVIDWTIEAFQRAGCGTIMVVRDPDNEHAI
CEEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEEEEHHHHHHCCCCEEEEEECCCCCHHH
EALAPYPELLLADGGPSRSESVRNGLIALQEIGVERVLIHDAARPCVCPQVIQQVLDALD
HHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHC
DTPAAAPGLAVTDALWTGADGHVTGTQDRSALFAAQTPQGFHFDAILAAHMRHDGTAADD
CCCCCCCCCEEHHHHHCCCCCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHH
VEVARQAGLAVRITPGDVNNIKITRPEDFSRAEHILRSTMDNIPDIRLGNGYDVHRFGPG
HHHHHHCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCC
DHVMLCGVQVPHERGLQGHSDADVGMHAVTDALYGAMAEGDIGRHFPPSDPQWKGAASDI
CEEEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
FLRHAVELARSKGFTINNVDCTLVCEYPKVGPHAEAMRARMAEIMGMDMGRLSIKATTSE
HHHHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHH
RLGFTGRKEGIAALATATLVRA
HCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLAVLIVAAGKGTRAGGGLAKQWRPLAGRLVIDWTIEAFQRAGCGTIMVVRDPDNEHAI
EEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEEEEHHHHHHCCCCEEEEEECCCCCHHH
EALAPYPELLLADGGPSRSESVRNGLIALQEIGVERVLIHDAARPCVCPQVIQQVLDALD
HHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHC
DTPAAAPGLAVTDALWTGADGHVTGTQDRSALFAAQTPQGFHFDAILAAHMRHDGTAADD
CCCCCCCCCEEHHHHHCCCCCCEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHH
VEVARQAGLAVRITPGDVNNIKITRPEDFSRAEHILRSTMDNIPDIRLGNGYDVHRFGPG
HHHHHHCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCC
DHVMLCGVQVPHERGLQGHSDADVGMHAVTDALYGAMAEGDIGRHFPPSDPQWKGAASDI
CEEEEEEEECCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
FLRHAVELARSKGFTINNVDCTLVCEYPKVGPHAEAMRARMAEIMGMDMGRLSIKATTSE
HHHHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHH
RLGFTGRKEGIAALATATLVRA
HCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA