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The map label for this gene is ntrB [H]

Identifier: 99081203

GI number: 99081203

Start: 1456636

End: 1457724

Strand: Reverse

Name: ntrB [H]

Synonym: TM1040_1362

Alternate gene names: 99081203

Gene position: 1457724-1456636 (Counterclockwise)

Preceding gene: 99081204

Following gene: 99081202

Centisome position: 45.54

GC content: 58.31

Gene sequence:

>1089_bases
ATGAATGATGCCTCGAATCCTCACAGCAACGACTGCGACATCTGGGCTTCGCTGCCGGTACCCGCGTTTCTGGTCGATGA
GCATAATTGCATTGAAGATGTGAACTCAGCTGCTGAGGGCTTTATCAACTCCTCGCGCAAGTCCCTGATTGGTAAGAAAA
TCTGGCAGAAGGTCGCGATTGAGTCCTCGATCGAACTGGCCTTTGACAAGGCTCGAGAGCAGGGCACGCCCCTATTTGTG
AATGATGTCGATGTCGGCGCCCGCGAACGCGCACCGTTGCAATGTGCTGTGCAGATTGCGCCGCTCCACGGGCATCCTGG
TCATATGCTGCTGATGCTCTCGCCGCGAGAGCTTGCGGGGCGGATGACGCAGACCCACTCCGCCAAATCGGCCGCGCAAT
CGGCGATCGGCATGGCAGAGATGCTGGCGCATGAAATCAAGAACCCTTTGGCGGGGATCACCGGTGCCGCGCAGCTTTTG
AGCATGAACCTCTCCACGGATGACCTTGAACTTACGGATCTAATCGTGAGTGAGAGCCGCCGGATCGTGAAGCTGCTGGA
GCAGGTGGAGCAGTTTGGAAATCTCTCCGAGCCGGACTTCAAACCGGTCAATCTGCATGATGTTCTGGACCGCGCGCGCC
GCTCCGCGCTGCTGGGCTTCGGCGCACATATGAAGATTGTCGAGAAATACGACCCCTCGCTGCCGCTGGCCTGGGGCGAC
CCGGATCAGCTGTTGCAGGTGGTTTTGAACCTTCTCAAGAATGCCTCAGAGGCCGCAGGCAGCGCGGGTGGCACCATTAC
GGTACGCACCTTTTATGAACACTCCTTCCGCCTGCGTCGCAGCGACGGACAGGGCAAGCTGCTGCCCTTGCAGATCGAGA
TCAGCGACGATGGGCCAGGGCTGCCGGAGAAGATCCGCGACGATATCTTTGAGCCTTTTGTCTCTGGCAAGGAGAACGGA
ACAGGTCTTGGCCTGGCCTTGGTCAGCAAGATCATCGGCGACCATAACGGTTGGATTTCCGTGCGCTCAGAGCCCGGCAA
CACGACCTTCAGACTGTCGCTTTCGCGCGTTCCGCGAGAGCAGAAATAA

Upstream 100 bases:

>100_bases
CTGCGGCCCTGCGGCGCGAGGTTCTGACGGCCAAATCCCCCTCTGATGTGTTGCGATTGCTCCCGAGTGCGCTTCAGGGA
ACTGAGCAGGAGACTGCCGC

Downstream 100 bases:

>100_bases
CTCAGGAGACCAAAGACATGGATGGCACCGTTCTTGTTGCAGATGATGACCGTACGATCCGCACTGTGCTCACACAGGCG
CTGACGCGGGCGGGGTGCAA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNDASNPHSNDCDIWASLPVPAFLVDEHNCIEDVNSAAEGFINSSRKSLIGKKIWQKVAIESSIELAFDKAREQGTPLFV
NDVDVGARERAPLQCAVQIAPLHGHPGHMLLMLSPRELAGRMTQTHSAKSAAQSAIGMAEMLAHEIKNPLAGITGAAQLL
SMNLSTDDLELTDLIVSESRRIVKLLEQVEQFGNLSEPDFKPVNLHDVLDRARRSALLGFGAHMKIVEKYDPSLPLAWGD
PDQLLQVVLNLLKNASEAAGSAGGTITVRTFYEHSFRLRRSDGQGKLLPLQIEISDDGPGLPEKIRDDIFEPFVSGKENG
TGLGLALVSKIIGDHNGWISVRSEPGNTTFRLSLSRVPREQK

Sequences:

>Translated_362_residues
MNDASNPHSNDCDIWASLPVPAFLVDEHNCIEDVNSAAEGFINSSRKSLIGKKIWQKVAIESSIELAFDKAREQGTPLFV
NDVDVGARERAPLQCAVQIAPLHGHPGHMLLMLSPRELAGRMTQTHSAKSAAQSAIGMAEMLAHEIKNPLAGITGAAQLL
SMNLSTDDLELTDLIVSESRRIVKLLEQVEQFGNLSEPDFKPVNLHDVLDRARRSALLGFGAHMKIVEKYDPSLPLAWGD
PDQLLQVVLNLLKNASEAAGSAGGTITVRTFYEHSFRLRRSDGQGKLLPLQIEISDDGPGLPEKIRDDIFEPFVSGKENG
TGLGLALVSKIIGDHNGWISVRSEPGNTTFRLSLSRVPREQK
>Mature_362_residues
MNDASNPHSNDCDIWASLPVPAFLVDEHNCIEDVNSAAEGFINSSRKSLIGKKIWQKVAIESSIELAFDKAREQGTPLFV
NDVDVGARERAPLQCAVQIAPLHGHPGHMLLMLSPRELAGRMTQTHSAKSAAQSAIGMAEMLAHEIKNPLAGITGAAQLL
SMNLSTDDLELTDLIVSESRRIVKLLEQVEQFGNLSEPDFKPVNLHDVLDRARRSALLGFGAHMKIVEKYDPSLPLAWGD
PDQLLQVVLNLLKNASEAAGSAGGTITVRTFYEHSFRLRRSDGQGKLLPLQIEISDDGPGLPEKIRDDIFEPFVSGKENG
TGLGLALVSKIIGDHNGWISVRSEPGNTTFRLSLSRVPREQK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=354, Percent_Identity=32.4858757062147, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788549, Length=351, Percent_Identity=27.9202279202279, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=29.5964125560538, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=30.379746835443, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=239, Percent_Identity=28.8702928870293, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790567, Length=212, Percent_Identity=33.0188679245283, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=27.5720164609054, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=271, Percent_Identity=26.1992619926199, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39484; Mature: 39484

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDASNPHSNDCDIWASLPVPAFLVDEHNCIEDVNSAAEGFINSSRKSLIGKKIWQKVAI
CCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESSIELAFDKAREQGTPLFVNDVDVGARERAPLQCAVQIAPLHGHPGHMLLMLSPRELAG
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCHHHHH
RMTQTHSAKSAAQSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSTDDLELTDLIVSESR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCH
RIVKLLEQVEQFGNLSEPDFKPVNLHDVLDRARRSALLGFGAHMKIVEKYDPSLPLAWGD
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC
PDQLLQVVLNLLKNASEAAGSAGGTITVRTFYEHSFRLRRSDGQGKLLPLQIEISDDGPG
HHHHHHHHHHHHHCHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCEEEEEEEECCCCCC
LPEKIRDDIFEPFVSGKENGTGLGLALVSKIIGDHNGWISVRSEPGNTTFRLSLSRVPRE
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEHHCCCCC
QK
CC
>Mature Secondary Structure
MNDASNPHSNDCDIWASLPVPAFLVDEHNCIEDVNSAAEGFINSSRKSLIGKKIWQKVAI
CCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESSIELAFDKAREQGTPLFVNDVDVGARERAPLQCAVQIAPLHGHPGHMLLMLSPRELAG
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCHHHHH
RMTQTHSAKSAAQSAIGMAEMLAHEIKNPLAGITGAAQLLSMNLSTDDLELTDLIVSESR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCH
RIVKLLEQVEQFGNLSEPDFKPVNLHDVLDRARRSALLGFGAHMKIVEKYDPSLPLAWGD
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC
PDQLLQVVLNLLKNASEAAGSAGGTITVRTFYEHSFRLRRSDGQGKLLPLQIEISDDGPG
HHHHHHHHHHHHHCHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCEEEEEEEECCCCCC
LPEKIRDDIFEPFVSGKENGTGLGLALVSKIIGDHNGWISVRSEPGNTTFRLSLSRVPRE
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEHHCCCCC
QK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]