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The map label for this gene is ntrC [H]

Identifier: 99081202

GI number: 99081202

Start: 1455239

End: 1456618

Strand: Reverse

Name: ntrC [H]

Synonym: TM1040_1361

Alternate gene names: 99081202

Gene position: 1456618-1455239 (Counterclockwise)

Preceding gene: 99081203

Following gene: 99081201

Centisome position: 45.51

GC content: 59.57

Gene sequence:

>1380_bases
ATGGATGGCACCGTTCTTGTTGCAGATGATGACCGTACGATCCGCACTGTGCTCACACAGGCGCTGACGCGGGCGGGGTG
CAAGGTCCATGCGACGTCCTCTCTCACAACGTTGATGCGCTGGGTTGGCGAAGGCAAAGGCGATGTTGTGATCTCCGACG
TGATGATGCCGGACGGCAATGGGCTGGAGATGCTCCCCAAGATTGCCCAGGACCGGCCCGGGCTGCCGGTCATCGTGATC
TCGGCGCAGAATACGATCATGACCGCCATCAAGGCTGCCGAAGCCGAAGCCTATGATTATCTGCCCAAACCTTTTGACCT
GCCTGAGCTGATGAAGCGCACGGCCAAGGCCCTCACCGAAAAGCGCAGCGCCCAGAGCACCAGCCATGTCGAGGTCGTCG
AGGAGCGACCCGATGAGCTGCCGCTGGTGGGGCGCACAGAGGTGATGCAGACGCTCTATCGTCTGATTGCGCGGGTTATG
AACACCGATATGCCGCTTTTGATCACGGGCGAGAGCGGGTCTGGGAAGTCCTTAATTGCCAAGGCAATACACGATTTCTC
GGACCGCAGGACCCTGCCATTTGTGGTCGCTGAGGCGTCTGATCTCATCGAGCTTGAAGGCCCCTCGCGCCTGCTCAGTC
AAGTGAAGGGCGGCACCTTGCTGGTCGATGAGCTGGTTGATCTGACCGATGAGGCTCAGGCGCGGCTTGTGCGGATGATG
GATTCGCCCGGAGACCACGCACCGCGCTTTATTGCGACCTCCAGTCAGCAAGATCTTGCTGAACAGATCGAAGCCGGCAA
ACTGCGTCAGGATCTCTACTACCGGATTTGCGGTACGGAGCTTGCGGTGCCTGCGTTGCGCGAACGGGTCGAGGACATCG
CACTTTTGTGTGAGCATTTCCTGACCCGCGCGGACGCCGAAGGGGGGCCGCGACGCATGCTGAGCGACGAAGCGCGGGAT
CTCTTGCGGCACTATTCTTGGCCTGGGAACGTGCGTCAGCTTGAAAATATCGTGAAAAGACTGGCGCTTACAGCGCGCGG
AGATACAATCAGCAAGTCAGAAGTCGCCGCAGCGCTTGGGCCGCAGAGCACCGGAGTTGCGCCCGTCATGGGGGGCGGGG
CAAGCAATGAAAAGCTCTCCGAATCTGTGGCGCGCCATCTGCAGCGATATTTCGATCTGCATGGCGACATGCTGCCACCA
CCGGGGCTGTATGCGCGGCTGCTGCGCGAAGTCGAAGCGCCGTTGATCGAGATCTCGCTTGCTGCAACCGGCGGAAATCA
GGCGAAATGTGCCGATCTCCTCGGGATCAACCGAAATACGCTGCGCAAGAAAATCACTGATCTCGATATAGAGGTGACAC
GCCGCCGCAAACTGATGTAA

Upstream 100 bases:

>100_bases
ACGGTTGGATTTCCGTGCGCTCAGAGCCCGGCAACACGACCTTCAGACTGTCGCTTTCGCGCGTTCCGCGAGAGCAGAAA
TAACTCAGGAGACCAAAGAC

Downstream 100 bases:

>100_bases
TATCGCCACAGCAGCGTTGCCCGGAGGTCAATAGGCCCATGGGGCAACGCGAGATAGTGTGGTGAGCCAAGAAGGCTCAA
CATCAACTGGGGGCAGGCGG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLTTLMRWVGEGKGDVVISDVMMPDGNGLEMLPKIAQDRPGLPVIVI
SAQNTIMTAIKAAEAEAYDYLPKPFDLPELMKRTAKALTEKRSAQSTSHVEVVEERPDELPLVGRTEVMQTLYRLIARVM
NTDMPLLITGESGSGKSLIAKAIHDFSDRRTLPFVVAEASDLIELEGPSRLLSQVKGGTLLVDELVDLTDEAQARLVRMM
DSPGDHAPRFIATSSQQDLAEQIEAGKLRQDLYYRICGTELAVPALRERVEDIALLCEHFLTRADAEGGPRRMLSDEARD
LLRHYSWPGNVRQLENIVKRLALTARGDTISKSEVAAALGPQSTGVAPVMGGGASNEKLSESVARHLQRYFDLHGDMLPP
PGLYARLLREVEAPLIEISLAATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM

Sequences:

>Translated_459_residues
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLTTLMRWVGEGKGDVVISDVMMPDGNGLEMLPKIAQDRPGLPVIVI
SAQNTIMTAIKAAEAEAYDYLPKPFDLPELMKRTAKALTEKRSAQSTSHVEVVEERPDELPLVGRTEVMQTLYRLIARVM
NTDMPLLITGESGSGKSLIAKAIHDFSDRRTLPFVVAEASDLIELEGPSRLLSQVKGGTLLVDELVDLTDEAQARLVRMM
DSPGDHAPRFIATSSQQDLAEQIEAGKLRQDLYYRICGTELAVPALRERVEDIALLCEHFLTRADAEGGPRRMLSDEARD
LLRHYSWPGNVRQLENIVKRLALTARGDTISKSEVAAALGPQSTGVAPVMGGGASNEKLSESVARHLQRYFDLHGDMLPP
PGLYARLLREVEAPLIEISLAATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM
>Mature_459_residues
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLTTLMRWVGEGKGDVVISDVMMPDGNGLEMLPKIAQDRPGLPVIVI
SAQNTIMTAIKAAEAEAYDYLPKPFDLPELMKRTAKALTEKRSAQSTSHVEVVEERPDELPLVGRTEVMQTLYRLIARVM
NTDMPLLITGESGSGKSLIAKAIHDFSDRRTLPFVVAEASDLIELEGPSRLLSQVKGGTLLVDELVDLTDEAQARLVRMM
DSPGDHAPRFIATSSQQDLAEQIEAGKLRQDLYYRICGTELAVPALRERVEDIALLCEHFLTRADAEGGPRRMLSDEARD
LLRHYSWPGNVRQLENIVKRLALTARGDTISKSEVAAALGPQSTGVAPVMGGGASNEKLSESVARHLQRYFDLHGDMLPP
PGLYARLLREVEAPLIEISLAATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=483, Percent_Identity=32.5051759834368, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1790437, Length=471, Percent_Identity=32.9087048832272, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788550, Length=479, Percent_Identity=27.9749478079332, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788905, Length=391, Percent_Identity=32.2250639386189, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87082152, Length=242, Percent_Identity=35.9504132231405, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789233, Length=355, Percent_Identity=31.2676056338028, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787583, Length=201, Percent_Identity=35.8208955223881, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082117, Length=242, Percent_Identity=32.6446280991736, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=31.9672131147541, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789087, Length=242, Percent_Identity=31.404958677686, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=29.6943231441048, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081858, Length=327, Percent_Identity=25.0764525993884, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786911, Length=125, Percent_Identity=35.2, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=130, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=26.7241379310345, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=28.9473684210526, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50345; Mature: 50345

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLTTLMRWVGEGKGDVVISDVMMPDGN
CCCCEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GLEMLPKIAQDRPGLPVIVISAQNTIMTAIKAAEAEAYDYLPKPFDLPELMKRTAKALTE
CHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KRSAQSTSHVEVVEERPDELPLVGRTEVMQTLYRLIARVMNTDMPLLITGESGSGKSLIA
HHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
KAIHDFSDRRTLPFVVAEASDLIELEGPSRLLSQVKGGTLLVDELVDLTDEAQARLVRMM
HHHHHHCCCCCCCEEEECCCCCEEECCHHHHHHHHCCCEEEHHHHHCCCHHHHHHHHHHH
DSPGDHAPRFIATSSQQDLAEQIEAGKLRQDLYYRICGTELAVPALRERVEDIALLCEHF
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTRADAEGGPRRMLSDEARDLLRHYSWPGNVRQLENIVKRLALTARGDTISKSEVAAALG
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
PQSTGVAPVMGGGASNEKLSESVARHLQRYFDLHGDMLPPPGLYARLLREVEAPLIEISL
CCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEE
AATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM
EECCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLTTLMRWVGEGKGDVVISDVMMPDGN
CCCCEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GLEMLPKIAQDRPGLPVIVISAQNTIMTAIKAAEAEAYDYLPKPFDLPELMKRTAKALTE
CHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KRSAQSTSHVEVVEERPDELPLVGRTEVMQTLYRLIARVMNTDMPLLITGESGSGKSLIA
HHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
KAIHDFSDRRTLPFVVAEASDLIELEGPSRLLSQVKGGTLLVDELVDLTDEAQARLVRMM
HHHHHHCCCCCCCEEEECCCCCEEECCHHHHHHHHCCCEEEHHHHHCCCHHHHHHHHHHH
DSPGDHAPRFIATSSQQDLAEQIEAGKLRQDLYYRICGTELAVPALRERVEDIALLCEHF
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTRADAEGGPRRMLSDEARDLLRHYSWPGNVRQLENIVKRLALTARGDTISKSEVAAALG
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
PQSTGVAPVMGGGASNEKLSESVARHLQRYFDLHGDMLPPPGLYARLLREVEAPLIEISL
CCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEE
AATGGNQAKCADLLGINRNTLRKKITDLDIEVTRRRKLM
EECCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]