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The map label for this gene is dus [H]

Identifier: 99081204

GI number: 99081204

Start: 1457721

End: 1458707

Strand: Reverse

Name: dus [H]

Synonym: TM1040_1363

Alternate gene names: 99081204

Gene position: 1458707-1457721 (Counterclockwise)

Preceding gene: 99081208

Following gene: 99081203

Centisome position: 45.57

GC content: 61.5

Gene sequence:

>987_bases
TTGTCCTTTTCAGTTGGACCCACACATCTCGATCCCCCCGTCGCTCTGGCGCCCATGGCCGGGATCACTGACCGCCCGTT
TCGGGATCTGGTGCGCTCTTTCGGGGCGGGCCTAGTGGTGAGCGAGATGGTCGCCAGTCAGGAGATGGTTCAAGCCAAGC
CCGGCGTGCGCGAGAAGGCGGAACTGAGCGCCGATGTGGAGAATACCTCGGTTCAGATCGCCGGGCGCGACGCGTATTGG
ATGGCAGAGGCCGCGCGTCAGGTGGCAGATCGTGGGGCGCGGATGATCGACATCAACATGGGATGTCCGGCAAAGAAAGT
GACCAACGGCTATTCGGGCTCTGCGCTCCTGAAGACCCCCGATCACGCGCTGTCGTTGATTGAGGCAGTCGTTCAGGCGG
TGGATGTGCCTGTCACGCTCAAGACCCGGTTGGGGTGGGACGATAACTGTCTCAATGCCGCTGATGTGGCGCGCCGCGCC
GAAGCCGCGGGTGTCCAGATGGTCACTATCCATGGTCGTACCCGGTGCCAGTTTTACAAAGGTCATGCTGACTGGGCTGC
GATCTCGGAGATCAAGAATGCGATCTCTGTTCCCTTGCTGGCCAATGGCGATATTGTCGATGCAAAAAGCGCGGGCAAGG
CGCTCTCAGACTCCGGAGCGGATGGCGTCATGATCGGGCGCGGTGTGCAGGGAAGACCCTGGCTTCTGGCTCAGATCGCG
CATGATCTCTGGGGCACGGCTGCTCCGGACGTTCCCGAAGGGCGCGCATTTATTGATCTGGTTTCAAAGCACTACGAGGC
GATGCTTGCCTTTTATGGGGCGGAGTTGGGCCTCCGCGTCGCGCGCAAGCACCTAGGCTGGTATATGGATGAGGCCGGGA
CACCTGCGGCCCTGCGGCGCGAGGTTCTGACGGCCAAATCCCCCTCTGATGTGTTGCGATTGCTCCCGAGTGCGCTTCAG
GGAACTGAGCAGGAGACTGCCGCATGA

Upstream 100 bases:

>100_bases
TAGGCATACAGTGTCATGAGCAGGGCTGGAATGGCGTGTTGGAATGGAGCATAGCTCCGATTCAAGCTAAAAAACACTCG
TATGCATAAGGATTAAGCAG

Downstream 100 bases:

>100_bases
ATGATGCCTCGAATCCTCACAGCAACGACTGCGACATCTGGGCTTCGCTGCCGGTACCCGCGTTTCTGGTCGATGAGCAT
AATTGCATTGAAGATGTGAA

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: Nitrogen regulation protein nifR3 [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSFSVGPTHLDPPVALAPMAGITDRPFRDLVRSFGAGLVVSEMVASQEMVQAKPGVREKAELSADVENTSVQIAGRDAYW
MAEAARQVADRGARMIDINMGCPAKKVTNGYSGSALLKTPDHALSLIEAVVQAVDVPVTLKTRLGWDDNCLNAADVARRA
EAAGVQMVTIHGRTRCQFYKGHADWAAISEIKNAISVPLLANGDIVDAKSAGKALSDSGADGVMIGRGVQGRPWLLAQIA
HDLWGTAAPDVPEGRAFIDLVSKHYEAMLAFYGAELGLRVARKHLGWYMDEAGTPAALRREVLTAKSPSDVLRLLPSALQ
GTEQETAA

Sequences:

>Translated_328_residues
MSFSVGPTHLDPPVALAPMAGITDRPFRDLVRSFGAGLVVSEMVASQEMVQAKPGVREKAELSADVENTSVQIAGRDAYW
MAEAARQVADRGARMIDINMGCPAKKVTNGYSGSALLKTPDHALSLIEAVVQAVDVPVTLKTRLGWDDNCLNAADVARRA
EAAGVQMVTIHGRTRCQFYKGHADWAAISEIKNAISVPLLANGDIVDAKSAGKALSDSGADGVMIGRGVQGRPWLLAQIA
HDLWGTAAPDVPEGRAFIDLVSKHYEAMLAFYGAELGLRVARKHLGWYMDEAGTPAALRREVLTAKSPSDVLRLLPSALQ
GTEQETAA
>Mature_327_residues
SFSVGPTHLDPPVALAPMAGITDRPFRDLVRSFGAGLVVSEMVASQEMVQAKPGVREKAELSADVENTSVQIAGRDAYWM
AEAARQVADRGARMIDINMGCPAKKVTNGYSGSALLKTPDHALSLIEAVVQAVDVPVTLKTRLGWDDNCLNAADVARRAE
AAGVQMVTIHGRTRCQFYKGHADWAAISEIKNAISVPLLANGDIVDAKSAGKALSDSGADGVMIGRGVQGRPWLLAQIAH
DLWGTAAPDVPEGRAFIDLVSKHYEAMLAFYGAELGLRVARKHLGWYMDEAGTPAALRREVLTAKSPSDVLRLLPSALQG
TEQETAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=229, Percent_Identity=31.4410480349345, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=32.9004329004329, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=32.8947368421053, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI40807366, Length=253, Percent_Identity=28.4584980237154, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=242, Percent_Identity=28.099173553719, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=43.0817610062893, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788462, Length=233, Percent_Identity=34.3347639484979, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI145693211, Length=264, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=30.7339449541284, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=289, Percent_Identity=26.643598615917, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=248, Percent_Identity=28.2258064516129, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=305, Percent_Identity=27.2131147540984, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=29.5652173913043, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=305, Percent_Identity=23.2786885245902, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=29.6875, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=29.6875, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585320, Length=320, Percent_Identity=28.75, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=31.4410480349345, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI45549423, Length=235, Percent_Identity=28.5106382978723, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34936; Mature: 34805

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVGPTHLDPPVALAPMAGITDRPFRDLVRSFGAGLVVSEMVASQEMVQAKPGVREKA
CCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHH
ELSADVENTSVQIAGRDAYWMAEAARQVADRGARMIDINMGCPAKKVTNGYSGSALLKTP
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCEEEECC
DHALSLIEAVVQAVDVPVTLKTRLGWDDNCLNAADVARRAEAAGVQMVTIHGRTRCQFYK
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCEEEEEE
GHADWAAISEIKNAISVPLLANGDIVDAKSAGKALSDSGADGVMIGRGVQGRPWLLAQIA
CCCHHHHHHHHHHHHCCCEEECCCEEECHHCCCHHCCCCCCCEEECCCCCCCCHHHHHHH
HDLWGTAAPDVPEGRAFIDLVSKHYEAMLAFYGAELGLRVARKHLGWYMDEAGTPAALRR
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHH
EVLTAKSPSDVLRLLPSALQGTEQETAA
HHHHCCCHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SFSVGPTHLDPPVALAPMAGITDRPFRDLVRSFGAGLVVSEMVASQEMVQAKPGVREKA
CCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHH
ELSADVENTSVQIAGRDAYWMAEAARQVADRGARMIDINMGCPAKKVTNGYSGSALLKTP
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCEEEECC
DHALSLIEAVVQAVDVPVTLKTRLGWDDNCLNAADVARRAEAAGVQMVTIHGRTRCQFYK
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCEEEEEE
GHADWAAISEIKNAISVPLLANGDIVDAKSAGKALSDSGADGVMIGRGVQGRPWLLAQIA
CCCHHHHHHHHHHHHCCCEEECCCEEECHHCCCHHCCCCCCCEEECCCCCCCCHHHHHHH
HDLWGTAAPDVPEGRAFIDLVSKHYEAMLAFYGAELGLRVARKHLGWYMDEAGTPAALRR
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHH
EVLTAKSPSDVLRLLPSALQGTEQETAA
HHHHCCCHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8355615 [H]