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The map label for this gene is braE [H]

Identifier: 99081117

GI number: 99081117

Start: 1374507

End: 1375832

Strand: Direct

Name: braE [H]

Synonym: TM1040_1276

Alternate gene names: 99081117

Gene position: 1374507-1375832 (Clockwise)

Preceding gene: 99081116

Following gene: 99081148

Centisome position: 42.94

GC content: 61.24

Gene sequence:

>1326_bases
ATGAGCGATACGTTGAAACATACGCTTCTGTTCGCCTTCGTCGGCCTGTTGCTGGTGCTCGAGGGGACCACAAATGCGTT
TGGCGTTTTCTCGGGATCGTGGAACTCTGCGTTGACCATCCTCAATATGGGGCTGATCTCGGCCATCATGGCGATTGGGG
TCAATCTGCAGTGGGGGTTTGCCGGGTTGTTCAACGTCGGCATCATGGGCTTTACCGCGCTTGGCGGGCTGGCCGTGGTT
TTGACCTCCATGCCGCCCACCCCCGGTGCCTGGCAGGCAGGTGGTTGGCAAATCCTGCTGGCGCTGGTGCTGGGTGCGGC
GACCATCGCAGCCGCAATCTACAGCTATCGGGTGATGGCGGCGGGCAAGGCGCGCACGCTGACCCTGCTTGCAATCCTGA
TCGTGGGATTTGTGGTTTACCGCGCAGCGCTGGATCCCGGTGTTGAAGCCATCGAGTCGATCAACCCCGCGCTTGAGGGC
AACATTGGCGGCTTTGGCCTGCCCGTGCTCCTGGCCTGGCCGCTGGGCGGTCTGCTGGCGGCGGGTGTTGCGTGGATGAT
CGGCAAGACAGCGCTGGGGCTGCGGTCGGACTATCTGGCGATCGCAACACTTGGCATTGCCGAGATCATCATTGCGGTGC
TGAAGAACGAGGACTGGCTGTCCCGAGGGGTCAAAAACGTCGTCGGCTTGCCGCGCCCCCTGCCCTATGAGGTTGATTTG
CAAGCAGATCCTGCGTTCGTGAACCAGGCTGCGGCTTGGGGCATGGATGCCACCACGGCCTCCACGCTCTACGTGAAACT
GGGCTATGCGCTCATGTTCACGGTCGTGCTGCTGGTCTTCCTCTGGATGGCACAAATGGCGCTCAAGAGCCCCTGGGGGC
GGATGATGCGCGCCATTCGGGACAATGAAGTGGCCGCCAACGCCATGGGCAAACATGTCACCCGGCGCCATCTGCAGGTC
TTCATCCTTGGTTCGGCCATTTGCGGCGTGGCTGGTGCGATGATGACGACGCTCGACAGCCAGTTGACACCCGGCACCTA
CAACCCGCTGCGGTTTACCTTCCTCATCTGGGTGATGGTGATCGTCGGAGGCTCCGGTAACAACTTCGGCGCGGTGCTTG
GCGGGCTCTTGGTGTGGTTCCTGTGGATCAAGGTGGAGCCGATGGGCGTGATGCTGATCGAGCTTGTGACCTCTGGCATG
TCCGAGGACAGCGGACTGCGCGCACATCTTCTGGAGAGTGCCTCACATATGCGCCTGTTCACCATGGGGCTGGTCTTGCT
GCTGGTGCTCCGGTTTAGCCCAAGGGGTCTGATCCCGGAGAAATAA

Upstream 100 bases:

>100_bases
CACAGCTTCTGAGCACGGATTACAAATTTGCGGTTTCCTTTGCGATCCTCGTCGTTGTGCTGCTGTTCAAACCCACCGGT
ATCTTCAAAGGAAAAGCGGT

Downstream 100 bases:

>100_bases
CCTCGGCAAGCCTGCCAACTTGAAGACAAACAAAAACGCCTGTCGAAATATTCGGCAGGCGTTTTCAATTTCCCGAGAGC
CTTAATCCCGAAGCTTTGTA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSDTLKHTLLFAFVGLLLVLEGTTNAFGVFSGSWNSALTILNMGLISAIMAIGVNLQWGFAGLFNVGIMGFTALGGLAVV
LTSMPPTPGAWQAGGWQILLALVLGAATIAAAIYSYRVMAAGKARTLTLLAILIVGFVVYRAALDPGVEAIESINPALEG
NIGGFGLPVLLAWPLGGLLAAGVAWMIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLSRGVKNVVGLPRPLPYEVDL
QADPAFVNQAAAWGMDATTASTLYVKLGYALMFTVVLLVFLWMAQMALKSPWGRMMRAIRDNEVAANAMGKHVTRRHLQV
FILGSAICGVAGAMMTTLDSQLTPGTYNPLRFTFLIWVMVIVGGSGNNFGAVLGGLLVWFLWIKVEPMGVMLIELVTSGM
SEDSGLRAHLLESASHMRLFTMGLVLLLVLRFSPRGLIPEK

Sequences:

>Translated_441_residues
MSDTLKHTLLFAFVGLLLVLEGTTNAFGVFSGSWNSALTILNMGLISAIMAIGVNLQWGFAGLFNVGIMGFTALGGLAVV
LTSMPPTPGAWQAGGWQILLALVLGAATIAAAIYSYRVMAAGKARTLTLLAILIVGFVVYRAALDPGVEAIESINPALEG
NIGGFGLPVLLAWPLGGLLAAGVAWMIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLSRGVKNVVGLPRPLPYEVDL
QADPAFVNQAAAWGMDATTASTLYVKLGYALMFTVVLLVFLWMAQMALKSPWGRMMRAIRDNEVAANAMGKHVTRRHLQV
FILGSAICGVAGAMMTTLDSQLTPGTYNPLRFTFLIWVMVIVGGSGNNFGAVLGGLLVWFLWIKVEPMGVMLIELVTSGM
SEDSGLRAHLLESASHMRLFTMGLVLLLVLRFSPRGLIPEK
>Mature_440_residues
SDTLKHTLLFAFVGLLLVLEGTTNAFGVFSGSWNSALTILNMGLISAIMAIGVNLQWGFAGLFNVGIMGFTALGGLAVVL
TSMPPTPGAWQAGGWQILLALVLGAATIAAAIYSYRVMAAGKARTLTLLAILIVGFVVYRAALDPGVEAIESINPALEGN
IGGFGLPVLLAWPLGGLLAAGVAWMIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLSRGVKNVVGLPRPLPYEVDLQ
ADPAFVNQAAAWGMDATTASTLYVKLGYALMFTVVLLVFLWMAQMALKSPWGRMMRAIRDNEVAANAMGKHVTRRHLQVF
ILGSAICGVAGAMMTTLDSQLTPGTYNPLRFTFLIWVMVIVGGSGNNFGAVLGGLLVWFLWIKVEPMGVMLIELVTSGMS
EDSGLRAHLLESASHMRLFTMGLVLLLVLRFSPRGLIPEK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=283, Percent_Identity=25.7950530035336, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46920; Mature: 46788

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLKHTLLFAFVGLLLVLEGTTNAFGVFSGSWNSALTILNMGLISAIMAIGVNLQWGF
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
AGLFNVGIMGFTALGGLAVVLTSMPPTPGAWQAGGWQILLALVLGAATIAAAIYSYRVMA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AGKARTLTLLAILIVGFVVYRAALDPGVEAIESINPALEGNIGGFGLPVLLAWPLGGLLA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHH
AGVAWMIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLSRGVKNVVGLPRPLPYEVDL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEC
QADPAFVNQAAAWGMDATTASTLYVKLGYALMFTVVLLVFLWMAQMALKSPWGRMMRAIR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
DNEVAANAMGKHVTRRHLQVFILGSAICGVAGAMMTTLDSQLTPGTYNPLRFTFLIWVMV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IVGGSGNNFGAVLGGLLVWFLWIKVEPMGVMLIELVTSGMSEDSGLRAHLLESASHMRLF
HHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TMGLVLLLVLRFSPRGLIPEK
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDTLKHTLLFAFVGLLLVLEGTTNAFGVFSGSWNSALTILNMGLISAIMAIGVNLQWGF
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
AGLFNVGIMGFTALGGLAVVLTSMPPTPGAWQAGGWQILLALVLGAATIAAAIYSYRVMA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AGKARTLTLLAILIVGFVVYRAALDPGVEAIESINPALEGNIGGFGLPVLLAWPLGGLLA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHH
AGVAWMIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLSRGVKNVVGLPRPLPYEVDL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEC
QADPAFVNQAAAWGMDATTASTLYVKLGYALMFTVVLLVFLWMAQMALKSPWGRMMRAIR
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
DNEVAANAMGKHVTRRHLQVFILGSAICGVAGAMMTTLDSQLTPGTYNPLRFTFLIWVMV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IVGGSGNNFGAVLGGLLVWFLWIKVEPMGVMLIELVTSGMSEDSGLRAHLLESASHMRLF
HHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TMGLVLLLVLRFSPRGLIPEK
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]