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The map label for this gene is fabI1 [H]

Identifier: 99080988

GI number: 99080988

Start: 1234809

End: 1235615

Strand: Reverse

Name: fabI1 [H]

Synonym: TM1040_1147

Alternate gene names: 99080988

Gene position: 1235615-1234809 (Counterclockwise)

Preceding gene: 99080997

Following gene: 99080987

Centisome position: 38.6

GC content: 58.12

Gene sequence:

>807_bases
ATGTCTAGTCAACTGATGGCCGGTAAGCGCGGCCTCATCATGGGCCTGGCCAACGACAAATCCATTGCGTGGGGCATCGC
CAAAGCCTGCGCCGAGGCCGGGGCGGAACTTGCGTTCTCCTATCAAGGAGACGCGCTCAAGAAACGCGTTGGACCACTGG
CCGAACAGCTGGGCAGCGACATTGTTCTGCCCTGCGATGTTTCAGACGAAGCATCCATCGACACACTCTTTGCGTCCCTC
GAGGAAAAATGGGGCAAGCTTGACTTTGTTGTGCATGCCATCGGCTTTTCCGACAAGAACGAGCTGCGTGGTCGCTATGT
CGACACCAGCCGCGCAAATTTCCAGATGACCATGGATATTTCCGTCTATTCCTTCACGGCCGTGGCGGCGCGTGCGGAAA
AGATGATGGAAGCCGGCGGCTCCATGCTGACGCTGACCTATTATGGCGCCGAACAAGTGATGCCGCATTATAATGTCATG
GGTGTCGCCAAGGCCGCGCTGGAAGCCTCTGTGAAATATCTCGCCGAGGATCTGGGCAAGGACGGCATCCGCGTCAACGC
GATTTCTGCCGGCCCGATCAAGACCCTGGCCGCCTCCGGCATTGGCGATTTCCGCTACATCATGAAGTGGAACGAATACA
ATTCGCCCCTGCGCCGCAATGTGACCACCGATGACGTGGGCAAATCCGCGCTCTACCTGCTGTCGGATCTCGCCTCCGGT
GTGACCGGCGAGAACCTGCATGTGGATGCGGGTTATCATGTGGTCGGCATGAAGGCCGTGGACGCCCCCGATATCGAGAA
AGGCTAA

Upstream 100 bases:

>100_bases
CTGCCCGATTGAACGCCCCCACAAGAGCGAGTAGGCTTGATGACTTTGCCCCAGCGGGCTAAAGGACATTCTATTAACCG
ATCACAGGCGGAGAACCTGC

Downstream 100 bases:

>100_bases
CCCCTGATGAGCACCAAGGATCCGAACCGCCTGCCCCACGAGAAGGGGTTTCACATCTCCTGGGATCAAATCCACCGAGA
CAGCCGCGCGCTGGCGTGGC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSSQLMAGKRGLIMGLANDKSIAWGIAKACAEAGAELAFSYQGDALKKRVGPLAEQLGSDIVLPCDVSDEASIDTLFASL
EEKWGKLDFVVHAIGFSDKNELRGRYVDTSRANFQMTMDISVYSFTAVAARAEKMMEAGGSMLTLTYYGAEQVMPHYNVM
GVAKAALEASVKYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPLRRNVTTDDVGKSALYLLSDLASG
VTGENLHVDAGYHVVGMKAVDAPDIEKG

Sequences:

>Translated_268_residues
MSSQLMAGKRGLIMGLANDKSIAWGIAKACAEAGAELAFSYQGDALKKRVGPLAEQLGSDIVLPCDVSDEASIDTLFASL
EEKWGKLDFVVHAIGFSDKNELRGRYVDTSRANFQMTMDISVYSFTAVAARAEKMMEAGGSMLTLTYYGAEQVMPHYNVM
GVAKAALEASVKYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPLRRNVTTDDVGKSALYLLSDLASG
VTGENLHVDAGYHVVGMKAVDAPDIEKG
>Mature_267_residues
SSQLMAGKRGLIMGLANDKSIAWGIAKACAEAGAELAFSYQGDALKKRVGPLAEQLGSDIVLPCDVSDEASIDTLFASLE
EKWGKLDFVVHAIGFSDKNELRGRYVDTSRANFQMTMDISVYSFTAVAARAEKMMEAGGSMLTLTYYGAEQVMPHYNVMG
VAKAALEASVKYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPLRRNVTTDDVGKSALYLLSDLASGV
TGENLHVDAGYHVVGMKAVDAPDIEKG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI59889578, Length=230, Percent_Identity=28.695652173913, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=53.1007751937985, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1789378, Length=252, Percent_Identity=28.968253968254, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=29.9595141700405, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=27.0588235294118, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=204, Percent_Identity=31.3725490196078, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644337, Length=196, Percent_Identity=28.0612244897959, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28739; Mature: 28608

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQLMAGKRGLIMGLANDKSIAWGIAKACAEAGAELAFSYQGDALKKRVGPLAEQLGSD
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCC
IVLPCDVSDEASIDTLFASLEEKWGKLDFVVHAIGFSDKNELRGRYVDTSRANFQMTMDI
EEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCHHCCCEEEECCCCCEEEEEEE
SVYSFTAVAARAEKMMEAGGSMLTLTYYGAEQVMPHYNVMGVAKAALEASVKYLAEDLGK
EHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
DGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPLRRNVTTDDVGKSALYLLSDLASG
CCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHCCCCCHHCCHHHHHHHHHHHCC
VTGENLHVDAGYHVVGMKAVDAPDIEKG
CCCCCEEEECCEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SSQLMAGKRGLIMGLANDKSIAWGIAKACAEAGAELAFSYQGDALKKRVGPLAEQLGSD
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCC
IVLPCDVSDEASIDTLFASLEEKWGKLDFVVHAIGFSDKNELRGRYVDTSRANFQMTMDI
EEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCHHCCCEEEECCCCCEEEEEEE
SVYSFTAVAARAEKMMEAGGSMLTLTYYGAEQVMPHYNVMGVAKAALEASVKYLAEDLGK
EHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
DGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPLRRNVTTDDVGKSALYLLSDLASG
CCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCHHHCCCCCHHCCHHHHHHHHHHHCC
VTGENLHVDAGYHVVGMKAVDAPDIEKG
CCCCCEEEECCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]