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The map label for this gene is pdhB [H]

Identifier: 99080919

GI number: 99080919

Start: 1153751

End: 1155127

Strand: Reverse

Name: pdhB [H]

Synonym: TM1040_1078

Alternate gene names: 99080919

Gene position: 1155127-1153751 (Counterclockwise)

Preceding gene: 99080920

Following gene: 99080918

Centisome position: 36.09

GC content: 61.73

Gene sequence:

>1377_bases
ATGGCAACTGAAATTCTGATGCCCGCCCTGTCGCCGACCATGGAGGAAGGCACACTGGCCAAATGGCTGGTCAAGGAAGG
CGATACCGTGAATTCCGGCGATATTCTAGCCGAGATCGAAACAGACAAGGCCACGATGGAATTCGAGGCCGTCGACGAAG
GTATCGTCGGCAAGATCCTCATCGACGAGGGCTCTGAGGGCGTAAAAGTGAACACGCCCATCGCGATCCTTGTTGAAGAA
GGGGAAAGCGTCGAGGATGCGGTCTCCTCCGCAGCCGCGCCTGCAGCCGAAGCCCCCGCCGCAGAGGCCGCAGCGCCCGC
GCCGGTCGCCGCGGCTGCCGCCACCCCGGCCGCACCCGAGGTGGATGAAAGCCCCGATTATCCCGAAGGCACCGAGATGG
TCAGCCAGACCGTCCGTGAAGCCCTGCGCGACGCCATGGCCGAAGAGATGCGCAGCAGCGAAGATGTCTTTGTGATGGGT
GAAGAAGTCGCCGAATATGAGGGCGCTTACAAGATCACCCAAGGTCTTCTGGACGAATTCGGCTCCAAACGCGTGATCGA
CACCCCGATCACCGAACATGGCTTTGCCGGGATCGCCACCGGCGCGGCCTTCGGGGGCCTGCGCCCGGTCGTGGAGTTCA
TGACCTTCAACTTCGCCATGCAAGCGATTGACCACATCATCAACTCTGCCGCCAAGACGCTCTATATGTCCGGTGGTCAG
ATGGGCGCACCGATGGTGTTCCGTGGCCCCAATGGCGCCGCCGCCCGCGTGGCTGCCCAGCACTCTCAGGACTATGCGGC
CTGGTACATGCAGATCCCCGGCCTCAAAGTGGCGATGCCCTACTCCGCTGCCGACGCCAAAGGCCTGCTGAAATCGGCGA
TCCGCGACCCCAACCCGGTGATTTTCCTCGAGAATGAGATCCTCTACGGCAAATCCTTTGAGGTGCCCAAGCTTGATGAC
TACACCGTGCCCTTCGGCAAGGCGAAGATCTGGCGCAAGGGCGATGATGTGACCATCGTGTCCTTCGGCATCGGCATGAC
CTACGCGCTTGACGCGGCGGACAAGCTGGCCGAGGACGGCATCAACGCCGAGGTCATCGACCTGCGCACCCTGCGCCCGA
TGGACCTGCCCACCGTCATCAAATCGGTGATGAAGACCAACCGTCTGGTCACTGTTGAGGAAGGCTGGCCGCAGGGCTCC
GTTGGCTCCTACATCGCCTCCGAGGTGATGCAACAGGCGTTCGACTATCTCGATGCGCCCGTCGCGGTCTGCACCGGCAA
GGACGTGCCGATGCCCTATGCTGCCAACCTCGAAAAACACGCGCTGATCACCACCGACGAGGTGATCGAAGCCGTGAAGC
AAGTGACCTACCGCTAA

Upstream 100 bases:

>100_bases
TCGCCAAAGAGAGCCCCGAGCCCGCGCTCGAGGAGCTTTGGACCGATATTTACGCCGACGATATTCCGCAAAAGAGCGCC
TGAGGGGAGAGACTGAGACT

Downstream 100 bases:

>100_bases
GGAGAGCAAGGATGCCCACTGAAATTCTCATGCCCGCCCTCTCTCCCACCATGGAGGAAGGCACGCTGGCGAAATGGCTC
GTCAAAGAAGGCGACACCGT

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MATEILMPALSPTMEEGTLAKWLVKEGDTVNSGDILAEIETDKATMEFEAVDEGIVGKILIDEGSEGVKVNTPIAILVEE
GESVEDAVSSAAAPAAEAPAAEAAAPAPVAAAAATPAAPEVDESPDYPEGTEMVSQTVREALRDAMAEEMRSSEDVFVMG
EEVAEYEGAYKITQGLLDEFGSKRVIDTPITEHGFAGIATGAAFGGLRPVVEFMTFNFAMQAIDHIINSAAKTLYMSGGQ
MGAPMVFRGPNGAAARVAAQHSQDYAAWYMQIPGLKVAMPYSAADAKGLLKSAIRDPNPVIFLENEILYGKSFEVPKLDD
YTVPFGKAKIWRKGDDVTIVSFGIGMTYALDAADKLAEDGINAEVIDLRTLRPMDLPTVIKSVMKTNRLVTVEEGWPQGS
VGSYIASEVMQQAFDYLDAPVAVCTGKDVPMPYAANLEKHALITTDEVIEAVKQVTYR

Sequences:

>Translated_458_residues
MATEILMPALSPTMEEGTLAKWLVKEGDTVNSGDILAEIETDKATMEFEAVDEGIVGKILIDEGSEGVKVNTPIAILVEE
GESVEDAVSSAAAPAAEAPAAEAAAPAPVAAAAATPAAPEVDESPDYPEGTEMVSQTVREALRDAMAEEMRSSEDVFVMG
EEVAEYEGAYKITQGLLDEFGSKRVIDTPITEHGFAGIATGAAFGGLRPVVEFMTFNFAMQAIDHIINSAAKTLYMSGGQ
MGAPMVFRGPNGAAARVAAQHSQDYAAWYMQIPGLKVAMPYSAADAKGLLKSAIRDPNPVIFLENEILYGKSFEVPKLDD
YTVPFGKAKIWRKGDDVTIVSFGIGMTYALDAADKLAEDGINAEVIDLRTLRPMDLPTVIKSVMKTNRLVTVEEGWPQGS
VGSYIASEVMQQAFDYLDAPVAVCTGKDVPMPYAANLEKHALITTDEVIEAVKQVTYR
>Mature_457_residues
ATEILMPALSPTMEEGTLAKWLVKEGDTVNSGDILAEIETDKATMEFEAVDEGIVGKILIDEGSEGVKVNTPIAILVEEG
ESVEDAVSSAAAPAAEAPAAEAAAPAPVAAAAATPAAPEVDESPDYPEGTEMVSQTVREALRDAMAEEMRSSEDVFVMGE
EVAEYEGAYKITQGLLDEFGSKRVIDTPITEHGFAGIATGAAFGGLRPVVEFMTFNFAMQAIDHIINSAAKTLYMSGGQM
GAPMVFRGPNGAAARVAAQHSQDYAAWYMQIPGLKVAMPYSAADAKGLLKSAIRDPNPVIFLENEILYGKSFEVPKLDDY
TVPFGKAKIWRKGDDVTIVSFGIGMTYALDAADKLAEDGINAEVIDLRTLRPMDLPTVIKSVMKTNRLVTVEEGWPQGSV
GSYIASEVMQQAFDYLDAPVAVCTGKDVPMPYAANLEKHALITTDEVIEAVKQVTYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=322, Percent_Identity=57.1428571428571, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI291084858, Length=322, Percent_Identity=53.7267080745342, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI4557353, Length=314, Percent_Identity=35.9872611464968, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI34101272, Length=314, Percent_Identity=35.9872611464968, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI203098753, Length=79, Percent_Identity=55.6962025316456, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI203098816, Length=79, Percent_Identity=55.6962025316456, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI31711992, Length=133, Percent_Identity=39.0977443609023, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI225637461, Length=303, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI225637459, Length=303, Percent_Identity=27.7227722772277, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI225637463, Length=303, Percent_Identity=28.0528052805281, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI260898739, Length=57, Percent_Identity=61.4035087719298, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI133778974, Length=161, Percent_Identity=31.6770186335404, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI205277463, Length=241, Percent_Identity=27.8008298755187, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI4507521, Length=241, Percent_Identity=27.8008298755187, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=324, Percent_Identity=62.6543209876543, Blast_Score=413, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17506935, Length=330, Percent_Identity=38.1818181818182, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=132, Percent_Identity=43.1818181818182, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539652, Length=315, Percent_Identity=26.3492063492064, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=58.5889570552147, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324258, Length=138, Percent_Identity=44.2028985507246, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321632, Length=99, Percent_Identity=42.4242424242424, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21358145, Length=323, Percent_Identity=59.4427244582043, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24650940, Length=323, Percent_Identity=59.4427244582043, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI160714832, Length=297, Percent_Identity=35.6902356902357, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI160714828, Length=297, Percent_Identity=35.6902356902357, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=65.1685393258427, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=65.1685393258427, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI20129315, Length=78, Percent_Identity=53.8461538461538, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24582497, Length=71, Percent_Identity=50.7042253521127, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45551847, Length=246, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550715, Length=246, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24645119, Length=246, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48890; Mature: 48759

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEILMPALSPTMEEGTLAKWLVKEGDTVNSGDILAEIETDKATMEFEAVDEGIVGKIL
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHCCCCEEEEE
IDEGSEGVKVNTPIAILVEEGESVEDAVSSAAAPAAEAPAAEAAAPAPVAAAAATPAAPE
EECCCCCEEECCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
VDESPDYPEGTEMVSQTVREALRDAMAEEMRSSEDVFVMGEEVAEYEGAYKITQGLLDEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHHHHHHHHH
GSKRVIDTPITEHGFAGIATGAAFGGLRPVVEFMTFNFAMQAIDHIINSAAKTLYMSGGQ
CCCCEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
MGAPMVFRGPNGAAARVAAQHSQDYAAWYMQIPGLKVAMPYSAADAKGLLKSAIRDPNPV
CCCCEEEECCCCCHHHHHHHCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCE
IFLENEILYGKSFEVPKLDDYTVPFGKAKIWRKGDDVTIVSFGIGMTYALDAADKLAEDG
EEEECEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEHHHHHHHHHCC
INAEVIDLRTLRPMDLPTVIKSVMKTNRLVTVEEGWPQGSVGSYIASEVMQQAFDYLDAP
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
VAVCTGKDVPMPYAANLEKHALITTDEVIEAVKQVTYR
EEEECCCCCCCCHHCCCCCCEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATEILMPALSPTMEEGTLAKWLVKEGDTVNSGDILAEIETDKATMEFEAVDEGIVGKIL
CCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHCCCCEEEEE
IDEGSEGVKVNTPIAILVEEGESVEDAVSSAAAPAAEAPAAEAAAPAPVAAAAATPAAPE
EECCCCCEEECCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
VDESPDYPEGTEMVSQTVREALRDAMAEEMRSSEDVFVMGEEVAEYEGAYKITQGLLDEF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHHHHHHHHH
GSKRVIDTPITEHGFAGIATGAAFGGLRPVVEFMTFNFAMQAIDHIINSAAKTLYMSGGQ
CCCCEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
MGAPMVFRGPNGAAARVAAQHSQDYAAWYMQIPGLKVAMPYSAADAKGLLKSAIRDPNPV
CCCCEEEECCCCCHHHHHHHCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCE
IFLENEILYGKSFEVPKLDDYTVPFGKAKIWRKGDDVTIVSFGIGMTYALDAADKLAEDG
EEEECEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEHHHHHHHHHCC
INAEVIDLRTLRPMDLPTVIKSVMKTNRLVTVEEGWPQGSVGSYIASEVMQQAFDYLDAP
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
VAVCTGKDVPMPYAANLEKHALITTDEVIEAVKQVTYR
EEEECCCCCCCCHHCCCCCCEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]