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The map label for this gene is pdhC [H]

Identifier: 99080918

GI number: 99080918

Start: 1152399

End: 1153739

Strand: Reverse

Name: pdhC [H]

Synonym: TM1040_1077

Alternate gene names: 99080918

Gene position: 1153739-1152399 (Counterclockwise)

Preceding gene: 99080919

Following gene: 99080915

Centisome position: 36.04

GC content: 65.32

Gene sequence:

>1341_bases
ATGCCCACTGAAATTCTCATGCCCGCCCTCTCTCCCACCATGGAGGAAGGCACGCTGGCGAAATGGCTCGTCAAAGAAGG
CGACACCGTCTCCTCTGGCGATCTGATTGCCGAAATCGAAACCGACAAGGCCACGATGGAATTCGAGGCCGTGGACGAAG
GTGTCGTTGGCAAGATCCTGATCGCCGAAGGCTCCGAGGGTGTGAAGGTCAACACCCCCATCGCGGTGCTGCTGGAAGAC
GGCGAAAGCGCCGATGATATCGACACATCGGCGGCAACACCGGAAGCGGCCCCCGCCGCTGACGCGGCTGCCGAGGAGGC
GCCTGCCGCCGCCGAGAAAGCCGCCGCCCCGGCTGCGGCAACCCCTGCCCCGGCAGCGCCCGCTGCGGCAGATGGCTCGC
GCATCTTTGCCTCGCCACTGGCGCGTCGCATCGCTGCCGACAAGGGACTCGACCTGAGCGCCATCAAAGGCTCCGGCCCC
CGTGGTCGCATCATCAAGGTGGACGTGGAAAACGCCACCGCCGCGCCCAAGGCCGACGCACAGACCGACGCGCAGGCTGC
CGCCGCCCCTGCGGCAAGTGCCTCCCCCGCGCCAGTCGCAGCCCCCGCCGGCCCCTCCGCCGATCAGGTGGCCAAGATGT
ACGAGGGCCGCAGCTTCGAGGAAGTCAAACTCGACGGGATGCGCAAGACCATTGCCGCGCGTCTCACCGAAGCCAAGCAG
ACCATCCCGCATTTCTACCTGCGCCGCGACATCCAGCTCGACGCGCTGTTGAAATTCCGCGCGCAGCTCAACAAGCAGCT
TGAAGGCCGCGGTGTGAAGCTCTCGGTCAACGACTTCATCATCAAGGCCGTGGCGCTGGCGCTGCAATCGGTGCCGGACG
CCAACGCCGTGTGGGCCGGGGATCGTGTGCTCAAGATGAAAGCCTCCGATGTGGCCGTTGCGGTCGCCATCGACGGCGGT
CTCTTCACGCCGGTCCTGCAAGACGCCGACATGAAGTCGCTGTCGGCCCTGTCGAGCGAAATGAAAGACCTCGCCACCCG
TGCGCGCGACCGCAAGCTTGCGCCGCATGAATACCAGGGCGGCTCCTTCGCGATCTCCAACCTCGGCATGTTCGGCATCG
ACAATTTCGACGCCATCGTGAACCCGCCGCATGCGGGTATTCTGGCCGTCGGCTCCGGCGTCAAGAAACCCGTGGTGGGC
GCCGATGGCGAGCTGACCGTTGCCACCGTCATGAGCGTCACCATGTCCGTGGATCACCGCGTGATCGACGGCGCATTGGG
CGCGGACCTCTTGAAGGCCATCGTCGACAATCTGGAAAACCCGATGGTGATGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGATGCCCTATGCTGCCAACCTCGAAAAACACGCGCTGATCACCACCGACGAGGTGATCGAAGCCGTGAAGCAAGTGACC
TACCGCTAAGGAGAGCAAGG

Downstream 100 bases:

>100_bases
TCGGCTGCGACTCACACCGAACGCAGAAGGGCCGGACACAGACGTCCGGCCCTTTTTCTATCTGCTTCTTCGATCTGCCG
GGCCCCGTCTTGAGCGCTGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPTEILMPALSPTMEEGTLAKWLVKEGDTVSSGDLIAEIETDKATMEFEAVDEGVVGKILIAEGSEGVKVNTPIAVLLED
GESADDIDTSAATPEAAPAADAAAEEAPAAAEKAAAPAAATPAPAAPAAADGSRIFASPLARRIAADKGLDLSAIKGSGP
RGRIIKVDVENATAAPKADAQTDAQAAAAPAASASPAPVAAPAGPSADQVAKMYEGRSFEEVKLDGMRKTIAARLTEAKQ
TIPHFYLRRDIQLDALLKFRAQLNKQLEGRGVKLSVNDFIIKAVALALQSVPDANAVWAGDRVLKMKASDVAVAVAIDGG
LFTPVLQDADMKSLSALSSEMKDLATRARDRKLAPHEYQGGSFAISNLGMFGIDNFDAIVNPPHAGILAVGSGVKKPVVG
ADGELTVATVMSVTMSVDHRVIDGALGADLLKAIVDNLENPMVMLA

Sequences:

>Translated_446_residues
MPTEILMPALSPTMEEGTLAKWLVKEGDTVSSGDLIAEIETDKATMEFEAVDEGVVGKILIAEGSEGVKVNTPIAVLLED
GESADDIDTSAATPEAAPAADAAAEEAPAAAEKAAAPAAATPAPAAPAAADGSRIFASPLARRIAADKGLDLSAIKGSGP
RGRIIKVDVENATAAPKADAQTDAQAAAAPAASASPAPVAAPAGPSADQVAKMYEGRSFEEVKLDGMRKTIAARLTEAKQ
TIPHFYLRRDIQLDALLKFRAQLNKQLEGRGVKLSVNDFIIKAVALALQSVPDANAVWAGDRVLKMKASDVAVAVAIDGG
LFTPVLQDADMKSLSALSSEMKDLATRARDRKLAPHEYQGGSFAISNLGMFGIDNFDAIVNPPHAGILAVGSGVKKPVVG
ADGELTVATVMSVTMSVDHRVIDGALGADLLKAIVDNLENPMVMLA
>Mature_445_residues
PTEILMPALSPTMEEGTLAKWLVKEGDTVSSGDLIAEIETDKATMEFEAVDEGVVGKILIAEGSEGVKVNTPIAVLLEDG
ESADDIDTSAATPEAAPAADAAAEEAPAAAEKAAAPAAATPAPAAPAAADGSRIFASPLARRIAADKGLDLSAIKGSGPR
GRIIKVDVENATAAPKADAQTDAQAAAAPAASASPAPVAAPAGPSADQVAKMYEGRSFEEVKLDGMRKTIAARLTEAKQT
IPHFYLRRDIQLDALLKFRAQLNKQLEGRGVKLSVNDFIIKAVALALQSVPDANAVWAGDRVLKMKASDVAVAVAIDGGL
FTPVLQDADMKSLSALSSEMKDLATRARDRKLAPHEYQGGSFAISNLGMFGIDNFDAIVNPPHAGILAVGSGVKKPVVGA
DGELTVATVMSVTMSVDHRVIDGALGADLLKAIVDNLENPMVMLA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=450, Percent_Identity=42.6666666666667, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI203098753, Length=466, Percent_Identity=38.1974248927039, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI203098816, Length=466, Percent_Identity=38.1974248927039, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=29.1284403669725, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=45.9119496855346, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=32.7586206896552, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1786946, Length=443, Percent_Identity=31.1512415349887, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=33.8862559241706, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=460, Percent_Identity=44.1304347826087, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17538894, Length=318, Percent_Identity=36.1635220125786, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=27.8801843317972, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=439, Percent_Identity=30.5239179954442, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=40.6521739130435, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6320352, Length=446, Percent_Identity=28.0269058295964, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6321632, Length=177, Percent_Identity=36.1581920903955, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI20129315, Length=454, Percent_Identity=41.1894273127753, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24582497, Length=447, Percent_Identity=40.4921700223714, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=29.5871559633028, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=31.7269076305221, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45952; Mature: 45821

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEILMPALSPTMEEGTLAKWLVKEGDTVSSGDLIAEIETDKATMEFEAVDEGVVGKIL
CCCHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCEEEEE
IAEGSEGVKVNTPIAVLLEDGESADDIDTSAATPEAAPAADAAAEEAPAAAEKAAAPAAA
EEECCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCC
TPAPAAPAAADGSRIFASPLARRIAADKGLDLSAIKGSGPRGRIIKVDVENATAAPKADA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCC
QTDAQAAAAPAASASPAPVAAPAGPSADQVAKMYEGRSFEEVKLDGMRKTIAARLTEAKQ
CCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TIPHFYLRRDIQLDALLKFRAQLNKQLEGRGVKLSVNDFIIKAVALALQSVPDANAVWAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECC
DRVLKMKASDVAVAVAIDGGLFTPVLQDADMKSLSALSSEMKDLATRARDRKLAPHEYQG
CEEEEEECCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSFAISNLGMFGIDNFDAIVNPPHAGILAVGSGVKKPVVGADGELTVATVMSVTMSVDHR
CCEEECCCCEECCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHH
VIDGALGADLLKAIVDNLENPMVMLA
HHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure 
PTEILMPALSPTMEEGTLAKWLVKEGDTVSSGDLIAEIETDKATMEFEAVDEGVVGKIL
CCHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCEEEEE
IAEGSEGVKVNTPIAVLLEDGESADDIDTSAATPEAAPAADAAAEEAPAAAEKAAAPAAA
EEECCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCC
TPAPAAPAAADGSRIFASPLARRIAADKGLDLSAIKGSGPRGRIIKVDVENATAAPKADA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCC
QTDAQAAAAPAASASPAPVAAPAGPSADQVAKMYEGRSFEEVKLDGMRKTIAARLTEAKQ
CCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TIPHFYLRRDIQLDALLKFRAQLNKQLEGRGVKLSVNDFIIKAVALALQSVPDANAVWAG
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECC
DRVLKMKASDVAVAVAIDGGLFTPVLQDADMKSLSALSSEMKDLATRARDRKLAPHEYQG
CEEEEEECCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSFAISNLGMFGIDNFDAIVNPPHAGILAVGSGVKKPVVGADGELTVATVMSVTMSVDHR
CCEEECCCCEECCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHH
VIDGALGADLLKAIVDNLENPMVMLA
HHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]