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The map label for this gene is ndhF [H]

Identifier: 99080673

GI number: 99080673

Start: 873956

End: 875506

Strand: Direct

Name: ndhF [H]

Synonym: TM1040_0832

Alternate gene names: 99080673

Gene position: 873956-875506 (Clockwise)

Preceding gene: 99080668

Following gene: 99080674

Centisome position: 27.3

GC content: 63.77

Gene sequence:

>1551_bases
ATGTCCTATCTCTTCGTGCCCCTCTTCAGCCCGCTGGTTCTGCTGGCCGCTGCCGTTTATGCGGCGCGCAATCCGGGCAA
GCGACCGGGACGGGTGCCGCTTGTGGCAGAATTGTCCGCGCTGGCGGCCTTTGGCGGCGCGCTGCTGTCCTCAGCGCTGC
TGGCGATGGAAGGCCCCGGCACCTCCGCGCTCGTGGGGGCACTTGGCATCGGGATCTCGGTGCGGCTGGATGTGGTGAGC
GTTGTGATGCTGGTACTGGTGAGCTTTATCGGCTGGATCGTCCTGCGCTATGCGCGCACCTATATGGACGGTGAAGAGCG
GCAGGGGGCCTTCACCGCATGGATGGCCGCGACTCTGGCGACGGTACTGCTGTTGGTGATGGCAGGCAATCTGGTACAGG
TCTTTGCAGCCTGGGTGATGACCGGCCTCCTTCTGAACCAACTGCTGCTGCATTATCCCGAACGCGCCACGGCGCAGCGG
GCGGCGCGCAAGAAGGCCTTTGTGGCCCGGCTGAGCGAAGTCGCCCTGGCAGTGTCATTTGTGCTCTTGGTTGTCGCGAC
CGGAACCACCGACATCGCGACCATTCTGGAAAGCGCGACGGCCACATGGCTGACCATGAGCGCGGCCCTGTTCCTGGCGA
TCGCTGCTGTTCTCGCATCGGCGCAGTTCCCGATGCACGGCTGGCTCACAGAGGTGATGGAAGCGCCCACACCGGTCTCG
GCGCTCCTGCATGCCGGGGTGATCAACGCTGGAGGTTTTCTTCTGATCCGCTTTGCTGACGTGATGCTTCTGGCCCCGGG
GGTGATGGCGCTCTTGGTGATGCTGGGCGGGTTCACGGCGCTCTTTGGCGGACTTGTGATGCTCACCCAGCCCGCAGTCA
AAACCTCGCTGGCGTGGTCCACCATTGCGCAGATGGCGTTCATGATGATGCAGTGTGGCCTTGCGCTCTTCCCGCTGGCG
CTGCTGCATATCGTGGCCCATTCGCTCTACAAGGCGCATGCCTTCCTGCGCTCGGGAGAAGCGGTGCGCAATGTCGCCAA
GATCCGCCGGCCCGGCCCGGTCGCGGTGCCAAGCCTGCGCAATGTGGCCCAGGCCTTTCTGAGCGCCATTGCGATCTACG
CGCTGGTCGGGCTTGGTCTTGGCTTTGACGGGAAATCGGCGCAGGCCATCGCGCTGGGTGTGATCTTGATCTTTGGTGTG
GCCTATCTTCTGGCGCAGGGCTTTGCTGACGAAGCACCGCGCGCCCTGATGCGTCGCACCGTGGTCTATGCGCTGGCCAC
ATCGCTGAGCTACTTCGCACTGCAGGTGATCGCCGTGTCGCTGACCAAGGGCACCTTGCCCGCAACCCCTGCACCTGGAC
CGCTGGAATGGGCACTGCTGGTGCTGGCTCTCCTAAGCTTCGGCCTGGTGGCGATTGCACAATCGACCTTCCCCCTCTGG
GCCACGCATCCAGCCGCGGCAGGTCTGCGGGTCCATCTGACCAACGGGCTTTATGCCAATGCGATCTTTGACAAGCTGCT
GGACGGTTGGTCCAAGCGCGCTGCAACCTGA

Upstream 100 bases:

>100_bases
AAGCGAACGAAAGCATCCAAACAATGAATTTTTATGGTCACGAGTTTTGACCTAGTTGATCTCCTCAGGTCGTTGACCCG
AAATTCTAAGGAGTGAGATC

Downstream 100 bases:

>100_bases
AAGGACACGAGACAATGTTTGCGAAACTAGAAACATATCCCGCCGCGTTGCTCGAACTGGTTGCAACCGCGAATGACGCA
GCCAAGGCCATCCCGCCCCT

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSYLFVPLFSPLVLLAAAVYAARNPGKRPGRVPLVAELSALAAFGGALLSSALLAMEGPGTSALVGALGIGISVRLDVVS
VVMLVLVSFIGWIVLRYARTYMDGEERQGAFTAWMAATLATVLLLVMAGNLVQVFAAWVMTGLLLNQLLLHYPERATAQR
AARKKAFVARLSEVALAVSFVLLVVATGTTDIATILESATATWLTMSAALFLAIAAVLASAQFPMHGWLTEVMEAPTPVS
ALLHAGVINAGGFLLIRFADVMLLAPGVMALLVMLGGFTALFGGLVMLTQPAVKTSLAWSTIAQMAFMMMQCGLALFPLA
LLHIVAHSLYKAHAFLRSGEAVRNVAKIRRPGPVAVPSLRNVAQAFLSAIAIYALVGLGLGFDGKSAQAIALGVILIFGV
AYLLAQGFADEAPRALMRRTVVYALATSLSYFALQVIAVSLTKGTLPATPAPGPLEWALLVLALLSFGLVAIAQSTFPLW
ATHPAAAGLRVHLTNGLYANAIFDKLLDGWSKRAAT

Sequences:

>Translated_516_residues
MSYLFVPLFSPLVLLAAAVYAARNPGKRPGRVPLVAELSALAAFGGALLSSALLAMEGPGTSALVGALGIGISVRLDVVS
VVMLVLVSFIGWIVLRYARTYMDGEERQGAFTAWMAATLATVLLLVMAGNLVQVFAAWVMTGLLLNQLLLHYPERATAQR
AARKKAFVARLSEVALAVSFVLLVVATGTTDIATILESATATWLTMSAALFLAIAAVLASAQFPMHGWLTEVMEAPTPVS
ALLHAGVINAGGFLLIRFADVMLLAPGVMALLVMLGGFTALFGGLVMLTQPAVKTSLAWSTIAQMAFMMMQCGLALFPLA
LLHIVAHSLYKAHAFLRSGEAVRNVAKIRRPGPVAVPSLRNVAQAFLSAIAIYALVGLGLGFDGKSAQAIALGVILIFGV
AYLLAQGFADEAPRALMRRTVVYALATSLSYFALQVIAVSLTKGTLPATPAPGPLEWALLVLALLSFGLVAIAQSTFPLW
ATHPAAAGLRVHLTNGLYANAIFDKLLDGWSKRAAT
>Mature_515_residues
SYLFVPLFSPLVLLAAAVYAARNPGKRPGRVPLVAELSALAAFGGALLSSALLAMEGPGTSALVGALGIGISVRLDVVSV
VMLVLVSFIGWIVLRYARTYMDGEERQGAFTAWMAATLATVLLLVMAGNLVQVFAAWVMTGLLLNQLLLHYPERATAQRA
ARKKAFVARLSEVALAVSFVLLVVATGTTDIATILESATATWLTMSAALFLAIAAVLASAQFPMHGWLTEVMEAPTPVSA
LLHAGVINAGGFLLIRFADVMLLAPGVMALLVMLGGFTALFGGLVMLTQPAVKTSLAWSTIAQMAFMMMQCGLALFPLAL
LHIVAHSLYKAHAFLRSGEAVRNVAKIRRPGPVAVPSLRNVAQAFLSAIAIYALVGLGLGFDGKSAQAIALGVILIFGVA
YLLAQGFADEAPRALMRRTVVYALATSLSYFALQVIAVSLTKGTLPATPAPGPLEWALLVLALLSFGLVAIAQSTFPLWA
THPAAAGLRVHLTNGLYANAIFDKLLDGWSKRAAT

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=288, Percent_Identity=32.2916666666667, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1788614, Length=305, Percent_Identity=33.4426229508197, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788829, Length=349, Percent_Identity=28.9398280802292, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788831, Length=297, Percent_Identity=28.6195286195286, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788827, Length=328, Percent_Identity=25.3048780487805, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54189; Mature: 54058

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLFVPLFSPLVLLAAAVYAARNPGKRPGRVPLVAELSALAAFGGALLSSALLAMEGPG
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSALVGALGIGISVRLDVVSVVMLVLVSFIGWIVLRYARTYMDGEERQGAFTAWMAATLA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVLLLVMAGNLVQVFAAWVMTGLLLNQLLLHYPERATAQRAARKKAFVARLSEVALAVSF
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLVVATGTTDIATILESATATWLTMSAALFLAIAAVLASAQFPMHGWLTEVMEAPTPVS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
ALLHAGVINAGGFLLIRFADVMLLAPGVMALLVMLGGFTALFGGLVMLTQPAVKTSLAWS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TIAQMAFMMMQCGLALFPLALLHIVAHSLYKAHAFLRSGEAVRNVAKIRRPGPVAVPSLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHH
NVAQAFLSAIAIYALVGLGLGFDGKSAQAIALGVILIFGVAYLLAQGFADEAPRALMRRT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVYALATSLSYFALQVIAVSLTKGTLPATPAPGPLEWALLVLALLSFGLVAIAQSTFPLW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
ATHPAAAGLRVHLTNGLYANAIFDKLLDGWSKRAAT
ECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYLFVPLFSPLVLLAAAVYAARNPGKRPGRVPLVAELSALAAFGGALLSSALLAMEGPG
CEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSALVGALGIGISVRLDVVSVVMLVLVSFIGWIVLRYARTYMDGEERQGAFTAWMAATLA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVLLLVMAGNLVQVFAAWVMTGLLLNQLLLHYPERATAQRAARKKAFVARLSEVALAVSF
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLVVATGTTDIATILESATATWLTMSAALFLAIAAVLASAQFPMHGWLTEVMEAPTPVS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
ALLHAGVINAGGFLLIRFADVMLLAPGVMALLVMLGGFTALFGGLVMLTQPAVKTSLAWS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TIAQMAFMMMQCGLALFPLALLHIVAHSLYKAHAFLRSGEAVRNVAKIRRPGPVAVPSLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHH
NVAQAFLSAIAIYALVGLGLGFDGKSAQAIALGVILIFGVAYLLAQGFADEAPRALMRRT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVYALATSLSYFALQVIAVSLTKGTLPATPAPGPLEWALLVLALLSFGLVAIAQSTFPLW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
ATHPAAAGLRVHLTNGLYANAIFDKLLDGWSKRAAT
ECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]