Click here to switch to the map view.

The map label for this gene is murB

Identifier: 99080526

GI number: 99080526

Start: 733268

End: 734194

Strand: Direct

Name: murB

Synonym: TM1040_0685

Alternate gene names: 99080526

Gene position: 733268-734194 (Clockwise)

Preceding gene: 99080525

Following gene: 99080527

Centisome position: 22.91

GC content: 61.7

Gene sequence:

>927_bases
ATGACCGACCTTCCTGCTGTGCGCGGGCGCCTGACGCCGAACCGAGACCTTTCCGATCTGACTTGGCTGCGTGTCGGAGG
TCCAGCGGATTACCTGTTCCAGCCAGCCGATCTGGAGGATCTGCAAGAATTCCTGCGGAATCTACCGGCAGATATGGACG
TCTTTCCCATGGGCGTTGGCTCCAATCTGATTGTGCGTGATGGCGGGTTGCGTTCGGTGGTGATCCGTCTTGGGCGCGGC
TTCAACGGGATCGAGGTCGACGGCGACACGGTGACTGCGGGCGCGGCGGCGCTTGATGCACATGTGGCACGAAAATCGGC
CGAGGCAGGAGTGGACCTGACGTTTCTGCGCACGATCCCCGGCAGTATTGGCGGCGCCGTGCGAATGAACGCGGGGTGCT
ATGGTTCGTATGTGGCGGATGTCTTTATGTCCGCGCAGGTGGTGCTGCGGGACGGCAGCCTTGCGACGCTTTCGGCAGAG
GATCTGCAGTTCAAGTACCGCCAGACCGAGCTGGCGCCCGGCGCAGTTCTCGTGTCCGCAACGCTGCGAGGCCCCAAGGG
GGACCCGGATGCCCTGGCGGCGCGCATGGAGGACCAGCTGCGCAAACGCGACGAGACCCAGCCGACAAAGGAACGCTCTG
CGGGGTCCACCTTCCGCAACCCGGCGGGGTTCTCGTCCACTGGCAAGGCGGATGATGTCATGGATCTCAAGGCCTGGAAG
GTGATTGACAACGCAGGCTTGCGCGGTGCCACCCTTGGAGGGGCGCAGATGAGTGAAAAACATTCCAACTTCATGATTAA
CGCCGGTGGCGCAACCGCCGCCGATCTGGAAGGATTGGGCGAAAATGTGCGAAAAAAGGTTTACGCGGACTCAGGCATTT
GGCTAGAATGGGAAATCATGCGTGTGGGCGATCCTAAGCGGTCGTGA

Upstream 100 bases:

>100_bases
GCACGGGAATGTCGTTCTGAAGGCGCCCGAGATCGGACAGAGGGGTTTTCCTCGCAATGGGCATGGCCTAAACCCTCGGG
GAAACCACAAGCGAGCCATC

Downstream 100 bases:

>100_bases
CCCTCGAAAACTCAAGTCAATTCATGAATTTGGGCCGATCAGCGGAATGTCTGATCCGGCTCGGTGGAGCGATCTGAGCA
GGGGCATGGTCGTTTGGCTG

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTDLPAVRGRLTPNRDLSDLTWLRVGGPADYLFQPADLEDLQEFLRNLPADMDVFPMGVGSNLIVRDGGLRSVVIRLGRG
FNGIEVDGDTVTAGAAALDAHVARKSAEAGVDLTFLRTIPGSIGGAVRMNAGCYGSYVADVFMSAQVVLRDGSLATLSAE
DLQFKYRQTELAPGAVLVSATLRGPKGDPDALAARMEDQLRKRDETQPTKERSAGSTFRNPAGFSSTGKADDVMDLKAWK
VIDNAGLRGATLGGAQMSEKHSNFMINAGGATAADLEGLGENVRKKVYADSGIWLEWEIMRVGDPKRS

Sequences:

>Translated_308_residues
MTDLPAVRGRLTPNRDLSDLTWLRVGGPADYLFQPADLEDLQEFLRNLPADMDVFPMGVGSNLIVRDGGLRSVVIRLGRG
FNGIEVDGDTVTAGAAALDAHVARKSAEAGVDLTFLRTIPGSIGGAVRMNAGCYGSYVADVFMSAQVVLRDGSLATLSAE
DLQFKYRQTELAPGAVLVSATLRGPKGDPDALAARMEDQLRKRDETQPTKERSAGSTFRNPAGFSSTGKADDVMDLKAWK
VIDNAGLRGATLGGAQMSEKHSNFMINAGGATAADLEGLGENVRKKVYADSGIWLEWEIMRVGDPKRS
>Mature_307_residues
TDLPAVRGRLTPNRDLSDLTWLRVGGPADYLFQPADLEDLQEFLRNLPADMDVFPMGVGSNLIVRDGGLRSVVIRLGRGF
NGIEVDGDTVTAGAAALDAHVARKSAEAGVDLTFLRTIPGSIGGAVRMNAGCYGSYVADVFMSAQVVLRDGSLATLSAED
LQFKYRQTELAPGAVLVSATLRGPKGDPDALAARMEDQLRKRDETQPTKERSAGSTFRNPAGFSSTGKADDVMDLKAWKV
IDNAGLRGATLGGAQMSEKHSNFMINAGGATAADLEGLGENVRKKVYADSGIWLEWEIMRVGDPKRS

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=288, Percent_Identity=26.0416666666667, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_SILST (Q1GIU8)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612680.1
- ProteinModelPortal:   Q1GIU8
- SMR:   Q1GIU8
- STRING:   Q1GIU8
- GeneID:   4077293
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0685
- NMPDR:   fig|292414.1.peg.2790
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- PhylomeDB:   Q1GIU8
- ProtClustDB:   PRK13905
- BioCyc:   SSP292414:TM1040_0685-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32918; Mature: 32786

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 167-167 ACT_SITE 216-216 ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPAVRGRLTPNRDLSDLTWLRVGGPADYLFQPADLEDLQEFLRNLPADMDVFPMGVG
CCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCC
SNLIVRDGGLRSVVIRLGRGFNGIEVDGDTVTAGAAALDAHVARKSAEAGVDLTFLRTIP
CEEEEECCCHHHHHHHHCCCCCCEEECCCEEECCHHHHHHHHHHHHHHCCCCEEEEECCC
GSIGGAVRMNAGCYGSYVADVFMSAQVVLRDGSLATLSAEDLQFKYRQTELAPGAVLVSA
CCCCCEEEECCCCHHHHHHHHHHCCEEEEECCCEEEEEHHHHHEEHHHHCCCCCEEEEEE
TLRGPKGDPDALAARMEDQLRKRDETQPTKERSAGSTFRNPAGFSSTGKADDVMDLKAWK
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHE
VIDNAGLRGATLGGAQMSEKHSNFMINAGGATAADLEGLGENVRKKVYADSGIWLEWEIM
EECCCCCCCEECCCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
RVGDPKRS
ECCCCCCC
>Mature Secondary Structure 
TDLPAVRGRLTPNRDLSDLTWLRVGGPADYLFQPADLEDLQEFLRNLPADMDVFPMGVG
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCC
SNLIVRDGGLRSVVIRLGRGFNGIEVDGDTVTAGAAALDAHVARKSAEAGVDLTFLRTIP
CEEEEECCCHHHHHHHHCCCCCCEEECCCEEECCHHHHHHHHHHHHHHCCCCEEEEECCC
GSIGGAVRMNAGCYGSYVADVFMSAQVVLRDGSLATLSAEDLQFKYRQTELAPGAVLVSA
CCCCCEEEECCCCHHHHHHHHHHCCEEEEECCCEEEEEHHHHHEEHHHHCCCCCEEEEEE
TLRGPKGDPDALAARMEDQLRKRDETQPTKERSAGSTFRNPAGFSSTGKADDVMDLKAWK
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHE
VIDNAGLRGATLGGAQMSEKHSNFMINAGGATAADLEGLGENVRKKVYADSGIWLEWEIM
EECCCCCCCEECCCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
RVGDPKRS
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA