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The map label for this gene is ddl

Identifier: 99080527

GI number: 99080527

Start: 734517

End: 735437

Strand: Direct

Name: ddl

Synonym: TM1040_0686

Alternate gene names: 99080527

Gene position: 734517-735437 (Clockwise)

Preceding gene: 99080526

Following gene: 99080528

Centisome position: 22.95

GC content: 62.11

Gene sequence:

>921_bases
GTGGGTGGAGCGAGCGGAACACTCCCGAAGGTTGCGATGTTGATGGGTGGCCCCTCGGCCGAGCGCGAGGTGTCTTTGTC
GACGGGGCGCGAATGCGCCCGCGCACTCAGAGGACAGGGCTACGAGGTGGTCGAGGTCGATGCAGGCCCCGACCTTGTAG
CGCAACTCAATGACATCAAACCGGATGTGGCTTTCAACGCTCTGCATGGGCGTTGGGGAGAAGACGGGTGTGTGCAGGGG
CTTCTTGAGTGGCTGCGCATCCCCTACACCCATTCCGGCGTGCTGGCCTCTGCACTGGCGATGGACAAGGAGCGCTCCAA
GGCGGCCTATCGTACCGCAGGCCTGCCGGTGGTCGACAGTGTGATCGCCGCCAAAGCAGACGTGATGGCCCGCCATGTGA
TCGCGCCGCCCTATGTGGTGAAGCCTAATAATGAGGGTTCGTCGGTTGGCATTTACATCGTTCACGAGGCCACCAACAGC
CCGCCGCAGCTCTCTGAAGAGATGCCTGCACAGGTGATGGTCGAGGCCTATGCGCCTGGTCGCGAGATGACAGTGACCGT
GATGGGGGATCGCGCGCTCTGTGTGACCGACATCCTCACCGATGGCTGGTACGACTATGAGGCCAAATATGCCACGGGTG
GCTCGCGCCATGTATTGCCTGCCGAGATCCCGGACGAAATCGCCGATCTATGCCATGATTACGCCCTGCGCGCCCATCAG
GTGCTTGGGTGTCGCGGGATTTCGCGTACGGATTTTCGCTGGGATGAGGCGCGCGGCGCTGATGGGCTGGTGCTGCTTGA
AACCAACACGCAACCGGGGATGACGCCGACCTCGTTATCGCCTGAACAGGCCGAGCATGTTGGTCTCACATTCGGGCAAC
TGTGCGCCTGGTTGGTGGAGGACGCGTCATGCGAGCGCTGA

Upstream 100 bases:

>100_bases
GATGCTAAGACAGACAAATTGCTGTGCGGGAGCCGGAAAACCGACCCCGTTATAATGATAAAATGGGCGAAAATGCCCGG
GTACAATTGAGGCAGTGATC

Downstream 100 bases:

>100_bases
CCTCTCGTTTGTCCCGCAGCCGCGCAGGTGAAAGCAAAGCTGACCCAGCGCCTTCACGATTGAAATACCGGATCCAGCGC
TGGATGTTGACGCCGGGCAT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MGGASGTLPKVAMLMGGPSAEREVSLSTGRECARALRGQGYEVVEVDAGPDLVAQLNDIKPDVAFNALHGRWGEDGCVQG
LLEWLRIPYTHSGVLASALAMDKERSKAAYRTAGLPVVDSVIAAKADVMARHVIAPPYVVKPNNEGSSVGIYIVHEATNS
PPQLSEEMPAQVMVEAYAPGREMTVTVMGDRALCVTDILTDGWYDYEAKYATGGSRHVLPAEIPDEIADLCHDYALRAHQ
VLGCRGISRTDFRWDEARGADGLVLLETNTQPGMTPTSLSPEQAEHVGLTFGQLCAWLVEDASCER

Sequences:

>Translated_306_residues
MGGASGTLPKVAMLMGGPSAEREVSLSTGRECARALRGQGYEVVEVDAGPDLVAQLNDIKPDVAFNALHGRWGEDGCVQG
LLEWLRIPYTHSGVLASALAMDKERSKAAYRTAGLPVVDSVIAAKADVMARHVIAPPYVVKPNNEGSSVGIYIVHEATNS
PPQLSEEMPAQVMVEAYAPGREMTVTVMGDRALCVTDILTDGWYDYEAKYATGGSRHVLPAEIPDEIADLCHDYALRAHQ
VLGCRGISRTDFRWDEARGADGLVLLETNTQPGMTPTSLSPEQAEHVGLTFGQLCAWLVEDASCER
>Mature_305_residues
GGASGTLPKVAMLMGGPSAEREVSLSTGRECARALRGQGYEVVEVDAGPDLVAQLNDIKPDVAFNALHGRWGEDGCVQGL
LEWLRIPYTHSGVLASALAMDKERSKAAYRTAGLPVVDSVIAAKADVMARHVIAPPYVVKPNNEGSSVGIYIVHEATNSP
PQLSEEMPAQVMVEAYAPGREMTVTVMGDRALCVTDILTDGWYDYEAKYATGGSRHVLPAEIPDEIADLCHDYALRAHQV
LGCRGISRTDFRWDEARGADGLVLLETNTQPGMTPTSLSPEQAEHVGLTFGQLCAWLVEDASCER

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=39.2156862745098, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=31.9088319088319, Blast_Score=134, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_SILST (Q1GIU7)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612681.1
- ProteinModelPortal:   Q1GIU7
- SMR:   Q1GIU7
- STRING:   Q1GIU7
- GeneID:   4077353
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0686
- NMPDR:   fig|292414.1.peg.2791
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   TDGWYDY
- PhylomeDB:   Q1GIU7
- ProtClustDB:   PRK01372
- BioCyc:   SSP292414:TM1040_0686-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32879; Mature: 32747

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGASGTLPKVAMLMGGPSAEREVSLSTGRECARALRGQGYEVVEVDAGPDLVAQLNDIK
CCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCC
PDVAFNALHGRWGEDGCVQGLLEWLRIPYTHSGVLASALAMDKERSKAAYRTAGLPVVDS
CCCEEEHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VIAAKADVMARHVIAPPYVVKPNNEGSSVGIYIVHEATNSPPQLSEEMPAQVMVEAYAPG
HHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEECCCCCCCCHHHHCCHHEEEEECCCC
REMTVTVMGDRALCVTDILTDGWYDYEAKYATGGSRHVLPAEIPDEIADLCHDYALRAHQ
CEEEEEEECCCEEEHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VLGCRGISRTDFRWDEARGADGLVLLETNTQPGMTPTSLSPEQAEHVGLTFGQLCAWLVE
HHHCCCCCCCCCCCHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
DASCER
HCCCCC
>Mature Secondary Structure 
GGASGTLPKVAMLMGGPSAEREVSLSTGRECARALRGQGYEVVEVDAGPDLVAQLNDIK
CCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCC
PDVAFNALHGRWGEDGCVQGLLEWLRIPYTHSGVLASALAMDKERSKAAYRTAGLPVVDS
CCCEEEHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VIAAKADVMARHVIAPPYVVKPNNEGSSVGIYIVHEATNSPPQLSEEMPAQVMVEAYAPG
HHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEECCCCCCCCHHHHCCHHEEEEECCCC
REMTVTVMGDRALCVTDILTDGWYDYEAKYATGGSRHVLPAEIPDEIADLCHDYALRAHQ
CEEEEEEECCCEEEHHHHHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VLGCRGISRTDFRWDEARGADGLVLLETNTQPGMTPTSLSPEQAEHVGLTFGQLCAWLVE
HHHCCCCCCCCCCCHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
DASCER
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA