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The map label for this gene is ghrA [H]

Identifier: 99080307

GI number: 99080307

Start: 485294

End: 486220

Strand: Reverse

Name: ghrA [H]

Synonym: TM1040_0466

Alternate gene names: 99080307

Gene position: 486220-485294 (Counterclockwise)

Preceding gene: 99080308

Following gene: 99080296

Centisome position: 15.19

GC content: 62.24

Gene sequence:

>927_bases
ATGATCAATGCGCTTTTTGCAGCCCGTCCGGAACGGTGGGACACCTATGAGCCAACCCTGCGCGCTGCGTTCGAGGCCGA
GGGGCTCGCAGTCGATCTGCGCCAGGATTTCGCGCCCGAGGACGTGGATTACATAGTCTATGCGCCCAATTCAGACCTTC
AGGACTTTCGCCCCTACACGCGCTGCAAAGCGGTCCTGAACCTCTGGGCCGGGGTGGAGAAGATCACCACCAATGAGACC
CTGACCCAACCGCTCTGCCGTATGGTCGACCCTGGCCTGACGCAATCCATGATCGAATGGGTCACGGGCCATGTGCTACG
GTATCATCTGGACATCGACCGCTCTCTCGCGCTGCAGAACAGCTGGTCGCCCTATATCCCGCCGCTTGCCGAAGAGCGCC
CCATCACCATTCTCGGCCTCGGCGCTCTGGGCCAGGCCTGCGCCACGGCTTTGACGCAACTCGGCTTTCCCGTCTCCGGA
TGGTCGCGCACCGCCAAATCTGTTGCGGGCGTGACCTGTCACCACGGACCAGAGGGCCTGAATGCAGCGCTTGCGGAGGC
GCAGATCGTTATCCTGCTCTTGCCGGATACGCCTCACACCCAAAACATCCTGAATGCGCAAAGCCTCGCTCGGCTGCCCA
AGGGCGCACGCATCATAAACCCCGGCCGCGGCCCCCTGATCGACGACGAGGCCCTGCTTGCGGCGCTCGAAACGGGGCAG
ATCGCACATGCGACCTTGGATGTGTTCCGGATTGAGCCCCTGCCCGAGGATCATCCCTATTGGGCCCATCCCAAAGTCAC
TGTGACCCCTCACATCGCGGCTGAAACCCGCCCCATCACGGCCTCACGCGTGATTGCGGAGAATATTCGCCGCGGCGAAG
CAGGTGAGGATTTTGTGCATCTCGTCGACCGGAACCGCGGCTATTGA

Upstream 100 bases:

>100_bases
AAGGAGAACCGGGCGAGCAAAGCACCATGTTCTTCTACGACCCGGCTGGAAACCCGATCGAGGTCAAAGGCTTTGCCGAT
ATGGACGGAGTGTTTGCAAC

Downstream 100 bases:

>100_bases
CGACGAAGGGCGCCCAACTTGGCGCCCCTCTTCATCTTTCCCAAATCTCCCCATCGGGGGCCGCGCCCCAGAAGGGGCGC
TCACTTGGCGGCCAGCCCAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MINALFAARPERWDTYEPTLRAAFEAEGLAVDLRQDFAPEDVDYIVYAPNSDLQDFRPYTRCKAVLNLWAGVEKITTNET
LTQPLCRMVDPGLTQSMIEWVTGHVLRYHLDIDRSLALQNSWSPYIPPLAEERPITILGLGALGQACATALTQLGFPVSG
WSRTAKSVAGVTCHHGPEGLNAALAEAQIVILLLPDTPHTQNILNAQSLARLPKGARIINPGRGPLIDDEALLAALETGQ
IAHATLDVFRIEPLPEDHPYWAHPKVTVTPHIAAETRPITASRVIAENIRRGEAGEDFVHLVDRNRGY

Sequences:

>Translated_308_residues
MINALFAARPERWDTYEPTLRAAFEAEGLAVDLRQDFAPEDVDYIVYAPNSDLQDFRPYTRCKAVLNLWAGVEKITTNET
LTQPLCRMVDPGLTQSMIEWVTGHVLRYHLDIDRSLALQNSWSPYIPPLAEERPITILGLGALGQACATALTQLGFPVSG
WSRTAKSVAGVTCHHGPEGLNAALAEAQIVILLLPDTPHTQNILNAQSLARLPKGARIINPGRGPLIDDEALLAALETGQ
IAHATLDVFRIEPLPEDHPYWAHPKVTVTPHIAAETRPITASRVIAENIRRGEAGEDFVHLVDRNRGY
>Mature_308_residues
MINALFAARPERWDTYEPTLRAAFEAEGLAVDLRQDFAPEDVDYIVYAPNSDLQDFRPYTRCKAVLNLWAGVEKITTNET
LTQPLCRMVDPGLTQSMIEWVTGHVLRYHLDIDRSLALQNSWSPYIPPLAEERPITILGLGALGQACATALTQLGFPVSG
WSRTAKSVAGVTCHHGPEGLNAALAEAQIVILLLPDTPHTQNILNAQSLARLPKGARIINPGRGPLIDDEALLAALETGQ
IAHATLDVFRIEPLPEDHPYWAHPKVTVTPHIAAETRPITASRVIAENIRRGEAGEDFVHLVDRNRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=312, Percent_Identity=26.2820512820513, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI6912396, Length=169, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI87081824, Length=255, Percent_Identity=42.7450980392157, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI87082289, Length=146, Percent_Identity=35.6164383561644, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789279, Length=152, Percent_Identity=28.2894736842105, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=165, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=165, Percent_Identity=32.7272727272727, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=152, Percent_Identity=29.6052631578947, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324055, Length=125, Percent_Identity=32, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320925, Length=185, Percent_Identity=27.027027027027, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI28571528, Length=175, Percent_Identity=32, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28574286, Length=171, Percent_Identity=30.4093567251462, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921140, Length=147, Percent_Identity=29.9319727891156, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45552429, Length=171, Percent_Identity=30.9941520467836, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28574284, Length=171, Percent_Identity=30.9941520467836, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585514, Length=171, Percent_Identity=30.9941520467836, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28574282, Length=171, Percent_Identity=30.9941520467836, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45551003, Length=171, Percent_Identity=30.9941520467836, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33921; Mature: 33921

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINALFAARPERWDTYEPTLRAAFEAEGLAVDLRQDFAPEDVDYIVYAPNSDLQDFRPYT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEHHHCCCCCCCCEEEECCCCCHHHCCHHH
RCKAVLNLWAGVEKITTNETLTQPLCRMVDPGLTQSMIEWVTGHVLRYHLDIDRSLALQN
HHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHCCC
SWSPYIPPLAEERPITILGLGALGQACATALTQLGFPVSGWSRTAKSVAGVTCHHGPEGL
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEECCCCCHH
NAALAEAQIVILLLPDTPHTQNILNAQSLARLPKGARIINPGRGPLIDDEALLAALETGQ
HHHHCCEEEEEEEECCCCCHHHHHCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCC
IAHATLDVFRIEPLPEDHPYWAHPKVTVTPHIAAETRPITASRVIAENIRRGEAGEDFVH
CCEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
LVDRNRGY
HHHCCCCC
>Mature Secondary Structure
MINALFAARPERWDTYEPTLRAAFEAEGLAVDLRQDFAPEDVDYIVYAPNSDLQDFRPYT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEHHHCCCCCCCCEEEECCCCCHHHCCHHH
RCKAVLNLWAGVEKITTNETLTQPLCRMVDPGLTQSMIEWVTGHVLRYHLDIDRSLALQN
HHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHCCC
SWSPYIPPLAEERPITILGLGALGQACATALTQLGFPVSGWSRTAKSVAGVTCHHGPEGL
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEECCCCCHH
NAALAEAQIVILLLPDTPHTQNILNAQSLARLPKGARIINPGRGPLIDDEALLAALETGQ
HHHHCCEEEEEEEECCCCCHHHHHCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCC
IAHATLDVFRIEPLPEDHPYWAHPKVTVTPHIAAETRPITASRVIAENIRRGEAGEDFVH
CCEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
LVDRNRGY
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA