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The map label for this gene is fdoI [H]

Identifier: 99080244

GI number: 99080244

Start: 412303

End: 413517

Strand: Direct

Name: fdoI [H]

Synonym: TM1040_0403

Alternate gene names: 99080244

Gene position: 412303-413517 (Clockwise)

Preceding gene: 99080243

Following gene: 99080245

Centisome position: 12.88

GC content: 60.0

Gene sequence:

>1215_bases
ATGCCGCGCCTGATTTTCGTATTCGTCCTCAAATTGGCCTTGCTTTGCGGCCTTTCTGTCAACGCGCAGGAGGCGAGCCC
AGCTGCGCCAGACCGCTCCGCCACCGGCGGCGCGCAGACGCTCGAGGATATTCTCGCGCGGCAACGTGGCGAGGATCTGG
ACGACAGCTTCCGCAGCGAGGCCACGGGCAACCCCGAGCTTGCCGCCGGGATTGAACAGCAGCTTGGCACCCTTGGAGGC
CAGTCCGACCCCGAACTCTGGCGCGCATTGCGCTATAATTCTGCCGACATCAGTGTTTCGGCCGGCGGCGATGTTGCGAC
GGTTCTGGTGCAGGATGGCGGCATGAGCTGGCTGAGCTTCCGCGAAGGACCGCTGCGCCAATATGGCGGGTGGCTCCTTT
TAGGCACGATTGCCGCGCTCGCCGTCTTCTTTGTGCTGCGGGGCCGGATCGGTATCGATGCAGACAAGACCGGACGCACC
GTGACCCGCTTTGCCTTTGTTGAGCGCATGGCCCATTGGACGCTTGCGGGATCGTTCATCCTGCTGGGTATCACCGGGCT
TTTGACGCTCTTTGGGCGCGTCGCAATCCTGCCCTACCTGGGCAAGGATGTGAATTCGGTGCTGCTCATCGGCTCCAAGT
GGATCCACAACTCCGTATCGTGGGCCTTCATGCTGGCGCTGATCGTGGTCTTTTTCATGTGGGTGGCGCATAACATCCCA
AACAAGCTCGATCTTGTCTGGCTCAAGCAGTTCGGTGGTATCATCGGCAGCAAGCACCCGCCCGCAAAGAAATTCAACGC
AGGGCAGAAGGTGATCTTCTGGTCGGTGATCTTGCTCGGGGCCTCGATTTCCGTCTCTGGTCTGTCGCTGCTGTTTCCCT
TCGAGATGCCGCTCTTTGCCAAGACCTTTGCCGCTCTGAACAGCACGGGAATCCCGGAATTGCTGGGCTTTGGGGTTCTT
CCCACCGAGCTTACGCCACATGGCGAAATGCAGCTTGCACAGCTCTGGCACGCCATTGTCGGCTTTGTCCTTATGGCGAT
CATCATCGCGCATATTTATATCGGCTCTGTCGGCATGGAAGGCGCCTATGATGCCATGGGGTCGGGCGAAGTGGATGAGG
CCTGGGCCCATCAGCACCATTCGATCTGGCTCGAAGAAGTGAAGGAAAAAGAGCGCGCAAAAGGCAGCGACGCGAGCGCC
ACTCCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CCAAAACGCCTACAACGGGAGGCGGCCCGCCGTTTGGCGGGCCCCTTCCGCTATACGAATAATTCATCTTAACAAATCCG
GCTCAACGGGAGGTCCATCC

Downstream 100 bases:

>100_bases
CGCGATGCTGCGGCGACTGGCTCTTGGCCTGCTCCTCTGCGCCGCCTCTGGGGTGAGCGCGGAGGAGTTCCAGACCCTCA
AAGGACACGGAGGGCCGATC

Product: formate dehydrogenase gamma subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPRLIFVFVLKLALLCGLSVNAQEASPAAPDRSATGGAQTLEDILARQRGEDLDDSFRSEATGNPELAAGIEQQLGTLGG
QSDPELWRALRYNSADISVSAGGDVATVLVQDGGMSWLSFREGPLRQYGGWLLLGTIAALAVFFVLRGRIGIDADKTGRT
VTRFAFVERMAHWTLAGSFILLGITGLLTLFGRVAILPYLGKDVNSVLLIGSKWIHNSVSWAFMLALIVVFFMWVAHNIP
NKLDLVWLKQFGGIIGSKHPPAKKFNAGQKVIFWSVILLGASISVSGLSLLFPFEMPLFAKTFAALNSTGIPELLGFGVL
PTELTPHGEMQLAQLWHAIVGFVLMAIIIAHIYIGSVGMEGAYDAMGSGEVDEAWAHQHHSIWLEEVKEKERAKGSDASA
TPAE

Sequences:

>Translated_404_residues
MPRLIFVFVLKLALLCGLSVNAQEASPAAPDRSATGGAQTLEDILARQRGEDLDDSFRSEATGNPELAAGIEQQLGTLGG
QSDPELWRALRYNSADISVSAGGDVATVLVQDGGMSWLSFREGPLRQYGGWLLLGTIAALAVFFVLRGRIGIDADKTGRT
VTRFAFVERMAHWTLAGSFILLGITGLLTLFGRVAILPYLGKDVNSVLLIGSKWIHNSVSWAFMLALIVVFFMWVAHNIP
NKLDLVWLKQFGGIIGSKHPPAKKFNAGQKVIFWSVILLGASISVSGLSLLFPFEMPLFAKTFAALNSTGIPELLGFGVL
PTELTPHGEMQLAQLWHAIVGFVLMAIIIAHIYIGSVGMEGAYDAMGSGEVDEAWAHQHHSIWLEEVKEKERAKGSDASA
TPAE
>Mature_403_residues
PRLIFVFVLKLALLCGLSVNAQEASPAAPDRSATGGAQTLEDILARQRGEDLDDSFRSEATGNPELAAGIEQQLGTLGGQ
SDPELWRALRYNSADISVSAGGDVATVLVQDGGMSWLSFREGPLRQYGGWLLLGTIAALAVFFVLRGRIGIDADKTGRTV
TRFAFVERMAHWTLAGSFILLGITGLLTLFGRVAILPYLGKDVNSVLLIGSKWIHNSVSWAFMLALIVVFFMWVAHNIPN
KLDLVWLKQFGGIIGSKHPPAKKFNAGQKVIFWSVILLGASISVSGLSLLFPFEMPLFAKTFAALNSTGIPELLGFGVLP
TELTPHGEMQLAQLWHAIVGFVLMAIIIAHIYIGSVGMEGAYDAMGSGEVDEAWAHQHHSIWLEEVKEKERAKGSDASAT
PAE

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=242, Percent_Identity=26.8595041322314, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787750, Length=249, Percent_Identity=26.9076305220884, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 43639; Mature: 43508

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLIFVFVLKLALLCGLSVNAQEASPAAPDRSATGGAQTLEDILARQRGEDLDDSFRSE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHC
ATGNPELAAGIEQQLGTLGGQSDPELWRALRYNSADISVSAGGDVATVLVQDGGMSWLSF
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCHHHHH
REGPLRQYGGWLLLGTIAALAVFFVLRGRIGIDADKTGRTVTRFAFVERMAHWTLAGSFI
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLGITGLLTLFGRVAILPYLGKDVNSVLLIGSKWIHNSVSWAFMLALIVVFFMWVAHNIP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NKLDLVWLKQFGGIIGSKHPPAKKFNAGQKVIFWSVILLGASISVSGLSLLFPFEMPLFA
CHHHHHHHHHHCCHHCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
KTFAALNSTGIPELLGFGVLPTELTPHGEMQLAQLWHAIVGFVLMAIIIAHIYIGSVGME
HHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAYDAMGSGEVDEAWAHQHHSIWLEEVKEKERAKGSDASATPAE
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PRLIFVFVLKLALLCGLSVNAQEASPAAPDRSATGGAQTLEDILARQRGEDLDDSFRSE
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHC
ATGNPELAAGIEQQLGTLGGQSDPELWRALRYNSADISVSAGGDVATVLVQDGGMSWLSF
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCHHHHH
REGPLRQYGGWLLLGTIAALAVFFVLRGRIGIDADKTGRTVTRFAFVERMAHWTLAGSFI
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLGITGLLTLFGRVAILPYLGKDVNSVLLIGSKWIHNSVSWAFMLALIVVFFMWVAHNIP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NKLDLVWLKQFGGIIGSKHPPAKKFNAGQKVIFWSVILLGASISVSGLSLLFPFEMPLFA
CHHHHHHHHHHCCHHCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
KTFAALNSTGIPELLGFGVLPTELTPHGEMQLAQLWHAIVGFVLMAIIIAHIYIGSVGME
HHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAYDAMGSGEVDEAWAHQHHSIWLEEVKEKERAKGSDASATPAE
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]