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The map label for this gene is cynR [H]

Identifier: 99080054

GI number: 99080054

Start: 229195

End: 230097

Strand: Direct

Name: cynR [H]

Synonym: TM1040_0213

Alternate gene names: 99080054

Gene position: 229195-230097 (Clockwise)

Preceding gene: 99080052

Following gene: 99080056

Centisome position: 7.16

GC content: 61.46

Gene sequence:

>903_bases
ATGGTTATCATTTTGGTGCTCAATTCTACCTGGCTCGAAACCTTTACCGTGCTCGCCGAAGAAGGGCATTTCACCCGCGC
GGCGGCCCGCTTGAATATGACGCAGCCCGGAGTGTCCCAGCACCTGCGCAAGCTCGAGCAGCAATTGGGACGGGCGCTGA
TCTCGAAGGAGGGCAAGCGCTTCACCCTGACCTCCGCCGGGGAGGCAGTGCGCGACCTCGGGTTGGCGCGGCGTGTCGAG
GAGCAGGCGCTGCGCGATACAATTACGCGCGATGATCCGGACCGAGGGGAGCTGCGGATCGCATGCTCTGGCAGTTTTGC
GATGCTGTTTTACCCACGCGCCATCGCGTTGATGAAAGAGGCACCGGGGCTTGTGATCCATGTGGAGGCTACGCCGCAGG
CGCGCGTGCTAGAGGGTGTTCTGGCCGGGCATTTTGATCTTGGGATTGTCTCTGATGTCCCACACCATCCACGTGTCGAA
GCACAGCAACTGGGGCAGGAGGAACTGTGCTTGCTGTTGCCGGCTGAGGTCGAGGACCCGGTGTCTTTCGAGACGCTTGA
AGCCCTGGGACTGGTCGCACACCCGGATGTGGCCAACTATGCGGATGATCTGTTTTCACGTAACTTCCCCATAGATTTTG
CTGGGGCGGACCGGTTGAAGCTGCGCACTTATATCAACCAGATCAGCCAGATCCCGTCTCCGGTGGCCGCTGGGGTTGGC
TATACGCTGCTTCCGCGCAGTGGTCTTGCGGCGTTTGCGCAATCGGCACAGATCAGGACGGCGCGCCTGCCGCAGCCACG
GTATCGGGAGCTCTGGCGCATAACCCGTCGCGGACGTATCGCAAGCGCGCGGCTAAAGCGGGTCTTGGCGGTCGTCGAAG
ACATCGCCGCGACCTTGGGATGA

Upstream 100 bases:

>100_bases
GGTGGGTGTCATGATGATCTCCTTTCAGATTTGCTTGATGGAAATCTAGACACGCGCCACCCGTATGTGAAATTATCATA
TCGAATTAAAATGCAAATGA

Downstream 100 bases:

>100_bases
GATCTCTTCGCCTTGGCGTCCGGGCCGGAATGGGCGTGGTCTGCCTTGTGAAAGCGGATACCAAAGCGAGGCGCTTCGGG
ATCTCTATTTGAACTTGTCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MVIILVLNSTWLETFTVLAEEGHFTRAAARLNMTQPGVSQHLRKLEQQLGRALISKEGKRFTLTSAGEAVRDLGLARRVE
EQALRDTITRDDPDRGELRIACSGSFAMLFYPRAIALMKEAPGLVIHVEATPQARVLEGVLAGHFDLGIVSDVPHHPRVE
AQQLGQEELCLLLPAEVEDPVSFETLEALGLVAHPDVANYADDLFSRNFPIDFAGADRLKLRTYINQISQIPSPVAAGVG
YTLLPRSGLAAFAQSAQIRTARLPQPRYRELWRITRRGRIASARLKRVLAVVEDIAATLG

Sequences:

>Translated_300_residues
MVIILVLNSTWLETFTVLAEEGHFTRAAARLNMTQPGVSQHLRKLEQQLGRALISKEGKRFTLTSAGEAVRDLGLARRVE
EQALRDTITRDDPDRGELRIACSGSFAMLFYPRAIALMKEAPGLVIHVEATPQARVLEGVLAGHFDLGIVSDVPHHPRVE
AQQLGQEELCLLLPAEVEDPVSFETLEALGLVAHPDVANYADDLFSRNFPIDFAGADRLKLRTYINQISQIPSPVAAGVG
YTLLPRSGLAAFAQSAQIRTARLPQPRYRELWRITRRGRIASARLKRVLAVVEDIAATLG
>Mature_300_residues
MVIILVLNSTWLETFTVLAEEGHFTRAAARLNMTQPGVSQHLRKLEQQLGRALISKEGKRFTLTSAGEAVRDLGLARRVE
EQALRDTITRDDPDRGELRIACSGSFAMLFYPRAIALMKEAPGLVIHVEATPQARVLEGVLAGHFDLGIVSDVPHHPRVE
AQQLGQEELCLLLPAEVEDPVSFETLEALGLVAHPDVANYADDLFSRNFPIDFAGADRLKLRTYINQISQIPSPVAAGVG
YTLLPRSGLAAFAQSAQIRTARLPQPRYRELWRITRRGRIASARLKRVLAVVEDIAATLG

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=197, Percent_Identity=31.4720812182741, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=26.1016949152542, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787806, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=287, Percent_Identity=25.7839721254355, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=140, Percent_Identity=33.5714285714286, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=243, Percent_Identity=26.7489711934156, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788887, Length=177, Percent_Identity=27.1186440677966, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33069; Mature: 33069

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50931 HTH_LYSR ; PS00758 ARGE_DAPE_CPG2_1 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIILVLNSTWLETFTVLAEEGHFTRAAARLNMTQPGVSQHLRKLEQQLGRALISKEGKR
CEEEEEECCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
FTLTSAGEAVRDLGLARRVEEQALRDTITRDDPDRGELRIACSGSFAMLFYPRAIALMKE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEHHHHHHHHHHC
APGLVIHVEATPQARVLEGVLAGHFDLGIVSDVPHHPRVEAQQLGQEELCLLLPAEVEDP
CCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHEEEEECCCCCCC
VSFETLEALGLVAHPDVANYADDLFSRNFPIDFAGADRLKLRTYINQISQIPSPVAAGVG
CCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCHHHHCCC
YTLLPRSGLAAFAQSAQIRTARLPQPRYRELWRITRRGRIASARLKRVLAVVEDIAATLG
EEECCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIILVLNSTWLETFTVLAEEGHFTRAAARLNMTQPGVSQHLRKLEQQLGRALISKEGKR
CEEEEEECCHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
FTLTSAGEAVRDLGLARRVEEQALRDTITRDDPDRGELRIACSGSFAMLFYPRAIALMKE
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEHHHHHHHHHHC
APGLVIHVEATPQARVLEGVLAGHFDLGIVSDVPHHPRVEAQQLGQEELCLLLPAEVEDP
CCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHEEEEECCCCCCC
VSFETLEALGLVAHPDVANYADDLFSRNFPIDFAGADRLKLRTYINQISQIPSPVAAGVG
CCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCHHHHCCC
YTLLPRSGLAAFAQSAQIRTARLPQPRYRELWRITRRGRIASARLKRVLAVVEDIAATLG
EEECCCCHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]