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The map label for this gene is fabI2 [H]

Identifier: 99080021

GI number: 99080021

Start: 199436

End: 200230

Strand: Reverse

Name: fabI2 [H]

Synonym: TM1040_0180

Alternate gene names: 99080021

Gene position: 200230-199436 (Counterclockwise)

Preceding gene: 99080022

Following gene: 99080020

Centisome position: 6.26

GC content: 61.26

Gene sequence:

>795_bases
GTGACTGGAGTATTGACCGGCAAACGGGGCCTGATCATGGGCGTTGCAAACGAACGCTCTATCGCTTGGGGGATCGCCAA
GGCGATGCATGAGGCAGGGGCCGAGCTGGCCTTTACCTACCAGGGCGAAGCCTTCGGCAAACGTCTTGAGCCGCTTGCGG
CCTCGGTGGACAGCGACTTCATGGTGGACGTGGATGTCACCGACGATGCCTCGCTGGATACGGCGTTCGCACAACTGGGC
GCCCGCTGGCCAAAGATTGATTTCCTTGTCCACGCGATTGCGTATTCGGACAAAAACGAGCTCACCGGCCGGTTCATCAA
CACCAGTCGTGCGAACTTCAAGAACTCCATGGATATCTCTGCCTATTCCTTCATCGAGGTGGCGCGCCGCGCCTACCCGC
TGATGCAGGAAAACGGCGGCGGCACGATGATGACGCTCACCTACGGTGGCTCCAATCGCGTGACGCCGAACTACAATGTG
ATGGGTGTGGCCAAGGCCGCATTGGAAAGCGCCACGCGCTATCTGGCCAATGACCTTGGTCCCGATGGCATCCGCGTAAA
TGCGATTTCCCCCGGCCCGATGAAAACCCTCGCCGGGGCTGCGATCGGTGGTGCGCGCAAGACCTACAAGCATACCGAGC
AGAACGCTCCGCTGCGGTCGAACGCCACGCTTGAGGCCGTCGGCGGCACCGCCGTCTACCTGGCGTCCGACGCGGGCGCC
TATACCACGGGCGAGATCATCCGCGTTGATGGCGGCTTCCATGTGCTTGGCATGCCCCAACAAGAACACCTCTGA

Upstream 100 bases:

>100_bases
ATGTTGAAAACGCGGGACTCGACAGTGTCATGACCAACAGCTTCGGCTTTGGCGGCACCAACGGCTCCATGGTTCTGAGT
AAGTACAAGGAATAACGACC

Downstream 100 bases:

>100_bases
TATTACTCATAATATCATGGTCTTAAGGCGCCCTTCGGGGCGCCTTTTCTTTTTGTCACAAGCGATCTGAAGCCCGGCTC
TTTTATTTTATACCGAATCG

Product: enoyl-[acyl-carrier-protein] reductase [NADH]

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTGVLTGKRGLIMGVANERSIAWGIAKAMHEAGAELAFTYQGEAFGKRLEPLAASVDSDFMVDVDVTDDASLDTAFAQLG
ARWPKIDFLVHAIAYSDKNELTGRFINTSRANFKNSMDISAYSFIEVARRAYPLMQENGGGTMMTLTYGGSNRVTPNYNV
MGVAKAALESATRYLANDLGPDGIRVNAISPGPMKTLAGAAIGGARKTYKHTEQNAPLRSNATLEAVGGTAVYLASDAGA
YTTGEIIRVDGGFHVLGMPQQEHL

Sequences:

>Translated_264_residues
MTGVLTGKRGLIMGVANERSIAWGIAKAMHEAGAELAFTYQGEAFGKRLEPLAASVDSDFMVDVDVTDDASLDTAFAQLG
ARWPKIDFLVHAIAYSDKNELTGRFINTSRANFKNSMDISAYSFIEVARRAYPLMQENGGGTMMTLTYGGSNRVTPNYNV
MGVAKAALESATRYLANDLGPDGIRVNAISPGPMKTLAGAAIGGARKTYKHTEQNAPLRSNATLEAVGGTAVYLASDAGA
YTTGEIIRVDGGFHVLGMPQQEHL
>Mature_263_residues
TGVLTGKRGLIMGVANERSIAWGIAKAMHEAGAELAFTYQGEAFGKRLEPLAASVDSDFMVDVDVTDDASLDTAFAQLGA
RWPKIDFLVHAIAYSDKNELTGRFINTSRANFKNSMDISAYSFIEVARRAYPLMQENGGGTMMTLTYGGSNRVTPNYNVM
GVAKAALESATRYLANDLGPDGIRVNAISPGPMKTLAGAAIGGARKTYKHTEQNAPLRSNATLEAVGGTAVYLASDAGAY
TTGEIIRVDGGFHVLGMPQQEHL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=28, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI10190704, Length=214, Percent_Identity=29.9065420560748, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI32483357, Length=167, Percent_Identity=26.3473053892216, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI126723750, Length=167, Percent_Identity=26.3473053892216, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787545, Length=261, Percent_Identity=48.2758620689655, Blast_Score=260, Evalue=7e-71,
Organism=Escherichia coli, GI1787905, Length=200, Percent_Identity=28.5, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367365, Length=259, Percent_Identity=27.4131274131274, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=25, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=228, Percent_Identity=27.6315789473684, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=209, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=178, Percent_Identity=29.2134831460674, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=113, Percent_Identity=32.7433628318584, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=257, Percent_Identity=26.8482490272374, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI23397609, Length=246, Percent_Identity=27.6422764227642, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571526, Length=196, Percent_Identity=28.0612244897959, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644337, Length=177, Percent_Identity=29.9435028248588, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28105; Mature: 27974

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVLTGKRGLIMGVANERSIAWGIAKAMHEAGAELAFTYQGEAFGKRLEPLAASVDSDF
CCCEEECCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCE
MVDVDVTDDASLDTAFAQLGARWPKIDFLVHAIAYSDKNELTGRFINTSRANFKNSMDIS
EEEEECCCCCCHHHHHHHHCCCCCHHHHHEEHHHCCCCCCCCEEEEECCCCCCCCCCCCH
AYSFIEVARRAYPLMQENGGGTMMTLTYGGSNRVTPNYNVMGVAKAALESATRYLANDLG
HHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PDGIRVNAISPGPMKTLAGAAIGGARKTYKHTEQNAPLRSNATLEAVGGTAVYLASDAGA
CCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEECCCEEEEEECCCCC
YTTGEIIRVDGGFHVLGMPQQEHL
EECCCEEEECCCEEEECCCHHHCC
>Mature Secondary Structure 
TGVLTGKRGLIMGVANERSIAWGIAKAMHEAGAELAFTYQGEAFGKRLEPLAASVDSDF
CCEEECCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCE
MVDVDVTDDASLDTAFAQLGARWPKIDFLVHAIAYSDKNELTGRFINTSRANFKNSMDIS
EEEEECCCCCCHHHHHHHHCCCCCHHHHHEEHHHCCCCCCCCEEEEECCCCCCCCCCCCH
AYSFIEVARRAYPLMQENGGGTMMTLTYGGSNRVTPNYNVMGVAKAALESATRYLANDLG
HHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PDGIRVNAISPGPMKTLAGAAIGGARKTYKHTEQNAPLRSNATLEAVGGTAVYLASDAGA
CCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEECCCEEEEEECCCCC
YTTGEIIRVDGGFHVLGMPQQEHL
EECCCEEEECCCEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]