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The map label for this gene is ilvE [C]

Identifier: 94984604

GI number: 94984604

Start: 516425

End: 517144

Strand: Reverse

Name: ilvE [C]

Synonym: Dgeo_0497

Alternate gene names: 94984604

Gene position: 517144-516425 (Counterclockwise)

Preceding gene: 94984605

Following gene: 94984603

Centisome position: 20.96

GC content: 70.69

Gene sequence:

>720_bases
ATGAGGCCGCTTCCCACCGAGGTCGACACGGGGGCCTGGCTCCACGGCGCCACGGCCTTTACGACCCTCCGCACGCACTG
GGGGAAGCCGCTGCTGTGGGAAGCGCACCTCGCGCGGCTGGCCGCAACCTGCGCCTGGCTGGGGCTCCCCGCACCGGAAG
GCGAGCCGCCCACCCTCGACCCGCTGCCCTGGGGCCTGTTGCGCCTGACCGCCACACCGGACGGTCTCTTTTGGTCGCAC
CGACTCCTCCAACCCGGTCCCCGTCCGGTGAAGGGCGTGCGCGTGTGTCTGACGAACGTGCAGGTTCACCCACAACTGGC
CGGGCACAAAACCGGGAACTATCTTCCCTCGCTGCTCGCGGGCCGCGAGGCCGAACGGCAGGGCGCGTTTGAGGGCTGGC
TGACGGACGCAGCAGGCAACATCGTAGACGGGAGCCGAACATCACCGCTGCTGGAACTGGGGGGGAGACTGGTCCTCCCG
GCAGGCGGCCTGCCGGGGATCACCCGCGCCGCCTTTCTGAAGGGCCAGGCGTTCGAGGAGCGGCCCGTCGCCGTATCCGA
GCTGCCGCACGTCACCCGCGCCTGGGTGTGCGGCAGCGGCGTGGGCGTGGTGCCGGTATGTGAAATCATAGGCGAAGGCT
GGCAGATGACGTCTCCTGCGGCGTGGCCAGAGGTGACCGATCCGGCGCTGCTCTGGCCCGCAGAGCAGGCGGGGCGGTGA

Upstream 100 bases:

>100_bases
TCCATCCCGAGAGCGTGCTGAGTCCGGCTGGGCGAAGATTGCTGGGAAACTGGCTGCGGCTCTCTGCCCTCCACACCGCC
CCCCAGCGCCAGGTCAGGCC

Downstream 100 bases:

>100_bases
GGTCCCCCCACTGCCCCGCCTGCTGCTCCCTGTGCTCAGTGGCCGTCCTGGCCGGTCCGTTCGCTCTGAAGGCGGCCGCC
GCCCGCAACCTCGTCCAGCT

Product: hypothetical protein

Products: 2-Oxoglutarate; L-Leucine; 3-Methyl-2-oxobutanoate; L-Glutamate; (S)-3-Methyl-2-oxopentanoate [C]

Alternate protein names: None

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRPLPTEVDTGAWLHGATAFTTLRTHWGKPLLWEAHLARLAATCAWLGLPAPEGEPPTLDPLPWGLLRLTATPDGLFWSH
RLLQPGPRPVKGVRVCLTNVQVHPQLAGHKTGNYLPSLLAGREAERQGAFEGWLTDAAGNIVDGSRTSPLLELGGRLVLP
AGGLPGITRAAFLKGQAFEERPVAVSELPHVTRAWVCGSGVGVVPVCEIIGEGWQMTSPAAWPEVTDPALLWPAEQAGR

Sequences:

>Translated_239_residues
MRPLPTEVDTGAWLHGATAFTTLRTHWGKPLLWEAHLARLAATCAWLGLPAPEGEPPTLDPLPWGLLRLTATPDGLFWSH
RLLQPGPRPVKGVRVCLTNVQVHPQLAGHKTGNYLPSLLAGREAERQGAFEGWLTDAAGNIVDGSRTSPLLELGGRLVLP
AGGLPGITRAAFLKGQAFEERPVAVSELPHVTRAWVCGSGVGVVPVCEIIGEGWQMTSPAAWPEVTDPALLWPAEQAGR
>Mature_239_residues
MRPLPTEVDTGAWLHGATAFTTLRTHWGKPLLWEAHLARLAATCAWLGLPAPEGEPPTLDPLPWGLLRLTATPDGLFWSH
RLLQPGPRPVKGVRVCLTNVQVHPQLAGHKTGNYLPSLLAGREAERQGAFEGWLTDAAGNIVDGSRTSPLLELGGRLVLP
AGGLPGITRAAFLKGQAFEERPVAVSELPHVTRAWVCGSGVGVVPVCEIIGEGWQMTSPAAWPEVTDPALLWPAEQAGR

Specific function: Acts On Leucine, Isoleucine And Valine. [C]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.6.1.42 [C]

Molecular weight: Translated: 25569; Mature: 25569

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLPTEVDTGAWLHGATAFTTLRTHWGKPLLWEAHLARLAATCAWLGLPAPEGEPPTLD
CCCCCCCCCCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PLPWGLLRLTATPDGLFWSHRLLQPGPRPVKGVRVCLTNVQVHPQLAGHKTGNYLPSLLA
CCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHEEEEECCEECCCCCCCCCCCHHHHHHC
GREAERQGAFEGWLTDAAGNIVDGSRTSPLLELGGRLVLPAGGLPGITRAAFLKGQAFEE
CCCHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHCCEEEEECCCCCCHHHHHHHCCCCCCC
RPVAVSELPHVTRAWVCGSGVGVVPVCEIIGEGWQMTSPAAWPEVTDPALLWPAEQAGR
CCCCHHHCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHCC
>Mature Secondary Structure
MRPLPTEVDTGAWLHGATAFTTLRTHWGKPLLWEAHLARLAATCAWLGLPAPEGEPPTLD
CCCCCCCCCCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PLPWGLLRLTATPDGLFWSHRLLQPGPRPVKGVRVCLTNVQVHPQLAGHKTGNYLPSLLA
CCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHEEEEECCEECCCCCCCCCCCHHHHHHC
GREAERQGAFEGWLTDAAGNIVDGSRTSPLLELGGRLVLPAGGLPGITRAAFLKGQAFEE
CCCHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHCCEEEEECCCCCCHHHHHHHCCCCCCC
RPVAVSELPHVTRAWVCGSGVGVVPVCEIIGEGWQMTSPAAWPEVTDPALLWPAEQAGR
CCCCHHHCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.13 {L-valine}} 2.7 {L-valine}} 1.26 {L-valine}} 0.2 {3-methyl-2-oxopentanoate}} 19 {L-methionine}} 2.2 {L-leucine}} 0.56 {L-leucine}} 0.97 {2-oxoglutarate}} 0.42 {L-isoleucine}} [C]

Substrates: 4-Methyl-2-oxopentanoate; L-Glutamate; 2-Oxoglutarate; L-Valine; L-Isoleucine [C]

Specific reaction: 4-Methyl-2-oxopentanoate + L-Glutamate --> 2-Oxoglutarate + L-Leucine 2-Oxoglutarate + L-Valine <==> 3-Methyl-2-oxobutanoate + L-Glutamate 2-Oxoglutarate + L-Isoleucine <==> (S)-3-Methyl-2-oxopentanoate + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA