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The map label for this gene is ispB [H]

Identifier: 94984603

GI number: 94984603

Start: 514701

End: 516428

Strand: Reverse

Name: ispB [H]

Synonym: Dgeo_0496

Alternate gene names: 94984603

Gene position: 516428-514701 (Counterclockwise)

Preceding gene: 94984604

Following gene: 94984602

Centisome position: 20.93

GC content: 68.23

Gene sequence:

>1728_bases
GTGAGGTCCCCCCACTGCCCCGCCTGCTGCTCCCTGTGCTCAGTGGCCGTCCTGGCCGGTCCGTTCGCTCTGAAGGCGGC
CGCCGCCCGCAACCTCGTCCAGCTTGAGGGCGCCGTAGCGCCGCATCAGCCTGGTGGCCTCCTCGGCGCGGGTGCCGTCG
GGATCGCGGGCAATCACCAGGATGAGGCCGCGCTTGATGCCGTCATAGAAGCGCTCGGCCTGCGCAGCGGGCACGCCCAT
CCGCCGCAGCAGCTTGACGTAGTCACCGTGGTCACTCCCGGTGAGGGCCCCAAAGAGGCCGCCCAGACCTGCACCGCCGA
TCACGCCGTACAGGATGGCCAGCAGGCCGCCTTCCTGGTAGATGCGGGTGCTGGGCACAATCAGCAGCAGGAGCCAGACT
GGGATGGTGAGGGCCGCACCGGCCAGTGCGCCCATCAGAATGCCGCGAATGACGCTTCCGGAACCGCCGGGCGCACCCTC
TTCCGGGCTGATGCCGGTCGCCTGCGCGAGATCATCCTCAGCCACCGCATCGGCCAGAGCAAAGCCAAGGTGGTCACGGT
CAAACCCGCGGTCCTGCAGGGCACCCAGCACACCCTGGACCTGCTGCGGCTCACGGAAAAGGGCAACGACGCTTTCCATC
CCAACGGTTTTACCACGCCCGGTGCAGGCGAAGCGAGGGACAAAGCGGGTATGGGGCGGCACTCTTCAGTCAAGGATACG
TTCGGCCCACCGGCGGTTTTCTATACTGCAGCCATGACTGGCGTGGCGGCCCTGATCCTTCCCGGCGCAGCGTTTGAGGC
GCGGCTGCGCGAGGTGCTGCGCTCGCGGGTAGAGTTTATCGAGCTGATCGGGGAAGACCTGGTGGCGGCGGGTGGCAAAC
GGGCTCGTCCCACCGTGACGTTCTTGGCCGCGCAGGCACTTGGGTTAGACGGCCAGGACCCGCGGTGGCAGACCGTCACC
GACCTTGCGGTCTGTATCGAGCTGCTGCACTCGGCCTCTTTGCTGCACGACGACCTGATTGACGATGCCGACACTCGCCG
GGGCCAGCAAGCCGCCTTCCGACGCTTCGGCAACGTGGTCAGTGTGATGAGCGGCGACTTCATGCTGGCGCGGCTGCTGA
CGCTGCTGGCTGCCATGCCCCAGGGCCCTGCGCTCACCCGCGCCTTTGGGCAGACGGCCTCCCAGATCTGCGAGGGCGAG
GTGCTGCAATTTCAGGTCGCGGCCTACGCTGAGTACGGCCTCGCCCCTTATCTGGACATCATTCACGGCAAGACGGCCGC
GCTGATCGAACTAGCGGCCTTCTCCCCTGCCCTGCTGCTGAATGCGCCGGAGGCGCAGCGGGCCGCCCTGGCGACCTTTG
GGCGCGAATACGGCCTGGCCTTTCAGATTCAAGACGACCTGCTCGACCTGATGGGCGATGAGGCCATCCTCGGCAAGCCG
GTGGGCGGCGATCTGCGCGAAGGCAAAGCGACGCTGCCAGTGCTCCACCTGCTGGACGGCCCCCAGCATGCCGAGGTTCG
GGCGGTGTTGGAACGCCGCGCGGCGAACCCCGGCGATGTGGAGCGGATCCGTGAGCTGGCCCGAACCCAAGGCGCGGTCG
AACGCACCCGTGAGGAGATTGGCCGCCGCGCCCGCCGCGCCTTGGAGGCCCTGGAACTCCTGCCTCCTTCCGAGGCGCGC
GCAGCCCTGGCCGCGCTCCCCCAGCGCGAGCTCGAGCGCACCCACTAG

Upstream 100 bases:

>100_bases
TATGTGAAATCATAGGCGAAGGCTGGCAGATGACGTCTCCTGCGGCGTGGCCAGAGGTGACCGATCCGGCGCTGCTCTGG
CCCGCAGAGCAGGCGGGGCG

Downstream 100 bases:

>100_bases
GCACTGCGGCCGCCTTGAGACAGCAGCGTCCAAGCCGTCCGGACAACCGGGTGCTTGCTCGCAACGGACCCACCCACACC
GCCACTGTCATTTGTCAAGA

Product: dimethylallyltransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MRSPHCPACCSLCSVAVLAGPFALKAAAARNLVQLEGAVAPHQPGGLLGAGAVGIAGNHQDEAALDAVIEALGLRSGHAH
PPQQLDVVTVVTPGEGPKEAAQTCTADHAVQDGQQAAFLVDAGAGHNQQQEPDWDGEGRTGQCAHQNAANDASGTAGRTL
FRADAGRLREIILSHRIGQSKAKVVTVKPAVLQGTQHTLDLLRLTEKGNDAFHPNGFTTPGAGEARDKAGMGRHSSVKDT
FGPPAVFYTAAMTGVAALILPGAAFEARLREVLRSRVEFIELIGEDLVAAGGKRARPTVTFLAAQALGLDGQDPRWQTVT
DLAVCIELLHSASLLHDDLIDDADTRRGQQAAFRRFGNVVSVMSGDFMLARLLTLLAAMPQGPALTRAFGQTASQICEGE
VLQFQVAAYAEYGLAPYLDIIHGKTAALIELAAFSPALLLNAPEAQRAALATFGREYGLAFQIQDDLLDLMGDEAILGKP
VGGDLREGKATLPVLHLLDGPQHAEVRAVLERRAANPGDVERIRELARTQGAVERTREEIGRRARRALEALELLPPSEAR
AALAALPQRELERTH

Sequences:

>Translated_575_residues
MRSPHCPACCSLCSVAVLAGPFALKAAAARNLVQLEGAVAPHQPGGLLGAGAVGIAGNHQDEAALDAVIEALGLRSGHAH
PPQQLDVVTVVTPGEGPKEAAQTCTADHAVQDGQQAAFLVDAGAGHNQQQEPDWDGEGRTGQCAHQNAANDASGTAGRTL
FRADAGRLREIILSHRIGQSKAKVVTVKPAVLQGTQHTLDLLRLTEKGNDAFHPNGFTTPGAGEARDKAGMGRHSSVKDT
FGPPAVFYTAAMTGVAALILPGAAFEARLREVLRSRVEFIELIGEDLVAAGGKRARPTVTFLAAQALGLDGQDPRWQTVT
DLAVCIELLHSASLLHDDLIDDADTRRGQQAAFRRFGNVVSVMSGDFMLARLLTLLAAMPQGPALTRAFGQTASQICEGE
VLQFQVAAYAEYGLAPYLDIIHGKTAALIELAAFSPALLLNAPEAQRAALATFGREYGLAFQIQDDLLDLMGDEAILGKP
VGGDLREGKATLPVLHLLDGPQHAEVRAVLERRAANPGDVERIRELARTQGAVERTREEIGRRARRALEALELLPPSEAR
AALAALPQRELERTH
>Mature_575_residues
MRSPHCPACCSLCSVAVLAGPFALKAAAARNLVQLEGAVAPHQPGGLLGAGAVGIAGNHQDEAALDAVIEALGLRSGHAH
PPQQLDVVTVVTPGEGPKEAAQTCTADHAVQDGQQAAFLVDAGAGHNQQQEPDWDGEGRTGQCAHQNAANDASGTAGRTL
FRADAGRLREIILSHRIGQSKAKVVTVKPAVLQGTQHTLDLLRLTEKGNDAFHPNGFTTPGAGEARDKAGMGRHSSVKDT
FGPPAVFYTAAMTGVAALILPGAAFEARLREVLRSRVEFIELIGEDLVAAGGKRARPTVTFLAAQALGLDGQDPRWQTVT
DLAVCIELLHSASLLHDDLIDDADTRRGQQAAFRRFGNVVSVMSGDFMLARLLTLLAAMPQGPALTRAFGQTASQICEGE
VLQFQVAAYAEYGLAPYLDIIHGKTAALIELAAFSPALLLNAPEAQRAALATFGREYGLAFQIQDDLLDLMGDEAILGKP
VGGDLREGKATLPVLHLLDGPQHAEVRAVLERRAANPGDVERIRELARTQGAVERTREEIGRRARRALEALELLPPSEAR
AALAALPQRELERTH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=312, Percent_Identity=31.0897435897436, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1789578, Length=317, Percent_Identity=36.5930599369085, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1786623, Length=221, Percent_Identity=33.4841628959276, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17505681, Length=309, Percent_Identity=30.7443365695793, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17508563, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319475, Length=297, Percent_Identity=29.6296296296296, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=239, Percent_Identity=25.9414225941423, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24651612, Length=278, Percent_Identity=32.7338129496403, Blast_Score=134, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 60811; Mature: 60811

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPHCPACCSLCSVAVLAGPFALKAAAARNLVQLEGAVAPHQPGGLLGAGAVGIAGNHQ
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEECCCCCC
DEAALDAVIEALGLRSGHAHPPQQLDVVTVVTPGEGPKEAAQTCTADHAVQDGQQAAFLV
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEE
DAGAGHNQQQEPDWDGEGRTGQCAHQNAANDASGTAGRTLFRADAGRLREIILSHRIGQS
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KAKVVTVKPAVLQGTQHTLDLLRLTEKGNDAFHPNGFTTPGAGEARDKAGMGRHSSVKDT
CCEEEEECHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
FGPPAVFYTAAMTGVAALILPGAAFEARLREVLRSRVEFIELIGEDLVAAGGKRARPTVT
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
FLAAQALGLDGQDPRWQTVTDLAVCIELLHSASLLHDDLIDDADTRRGQQAAFRRFGNVV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHH
SVMSGDFMLARLLTLLAAMPQGPALTRAFGQTASQICEGEVLQFQVAAYAEYGLAPYLDI
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHCCHHHHHH
IHGKTAALIELAAFSPALLLNAPEAQRAALATFGREYGLAFQIQDDLLDLMGDEAILGKP
HCCCHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHHHCEEEEEHHHHHHHHCCCHHCCCC
VGGDLREGKATLPVLHLLDGPQHAEVRAVLERRAANPGDVERIRELARTQGAVERTREEI
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GRRARRALEALELLPPSEARAALAALPQRELERTH
HHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MRSPHCPACCSLCSVAVLAGPFALKAAAARNLVQLEGAVAPHQPGGLLGAGAVGIAGNHQ
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEECCCCCC
DEAALDAVIEALGLRSGHAHPPQQLDVVTVVTPGEGPKEAAQTCTADHAVQDGQQAAFLV
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEE
DAGAGHNQQQEPDWDGEGRTGQCAHQNAANDASGTAGRTLFRADAGRLREIILSHRIGQS
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KAKVVTVKPAVLQGTQHTLDLLRLTEKGNDAFHPNGFTTPGAGEARDKAGMGRHSSVKDT
CCEEEEECHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
FGPPAVFYTAAMTGVAALILPGAAFEARLREVLRSRVEFIELIGEDLVAAGGKRARPTVT
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
FLAAQALGLDGQDPRWQTVTDLAVCIELLHSASLLHDDLIDDADTRRGQQAAFRRFGNVV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHH
SVMSGDFMLARLLTLLAAMPQGPALTRAFGQTASQICEGEVLQFQVAAYAEYGLAPYLDI
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHHHHHCCHHHHHH
IHGKTAALIELAAFSPALLLNAPEAQRAALATFGREYGLAFQIQDDLLDLMGDEAILGKP
HCCCHHHHHHHHHCCCHHEECCCHHHHHHHHHHHHHHCEEEEEHHHHHHHHCCCHHCCCC
VGGDLREGKATLPVLHLLDGPQHAEVRAVLERRAANPGDVERIRELARTQGAVERTREEI
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GRRARRALEALELLPPSEARAALAALPQRELERTH
HHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]