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The map label for this gene is gdhA [H]

Identifier: 94984602

GI number: 94984602

Start: 513141

End: 514385

Strand: Reverse

Name: gdhA [H]

Synonym: Dgeo_0495

Alternate gene names: 94984602

Gene position: 514385-513141 (Counterclockwise)

Preceding gene: 94984603

Following gene: 94984601

Centisome position: 20.85

GC content: 66.27

Gene sequence:

>1245_bases
ATGAGGGCATCAGGACTCAACTGGCAGGGCCTCATGGAACAGCTCCAGCAGGCACTGCCGTACAGCGAGGTCAGCGATCA
GTCGCTTGCCTACTTCAAATATCCCAAACGCACCCTGAGCGTAAACTTGCCGGTTCGGATGGACGACGGCACGGTGCGCG
TTTTTAAGGGCTACCGCACCGTTCATTCCACCGCCCGCGGCCCCAGCATGGGCGGCGTCCGCTTTAAGCCGGGTCTCAAC
GCGCACGAGTGCGAGGTGCTCGCCGCGATCATGACCCTCAAGGCCGCCGTGGCCGATCTGCCGCTGGGCGGCGCCAAGGG
CGGTGTGGATGTGGACCCGCAGCAGCTCAGCCCGCACGAGCTGGAGGGCTTGACCCGGCGCTACACCAGCGAACTGGTGG
AGCTGGTTGGCCCGAGCGAAGATATTCTGGCGCCGGACGTGGGCACCAGCCCACAGGTCATGGCCTGGATCCTGGACACC
TACGGGGAGAACACCGGCTCCACGTCGAGCGGCATGGTGGTGGGCAAGCCGCTGTCACTGGGCGGCAGCTACGGCAGCAA
AGACGCCCGGGGGCGCAGCGCCGCTCTCGTCACCGCACGGGTGCTGGAGGCGAGGGGTGAGAGCCTACAGAGCGCTCGTG
TGGCTGTGTACGGGTTCGGGGACGTGGGCCGCCGGGCCGCGCAGACCCTGGCGGCTCAAGGCGCCCTGGTGATCGCGGTT
TCGGACCAGAACGGGGCCACCTTTGCCAGCAGCGGCCTGGACCTGGACGCCCTCTCCAGCTACCGCGAGGCGCATGGCAG
CGTGCAGGGCTTTGCGACCGATATCACCCCCGAAGAGGTGATTGAGCTTGACGTGGACGTGCTGATGCTGGCCTATGACT
ACGGCAGCGTGAACGCGGGGAATGCCCACGCCGTCCGTGCCCGCTTTGTGGTGGAGGCGGCCAACCGTGCCGTCCTGCCC
GAGGCCGAACGCTTCCTGCAGGCCCAAGGCGTCACCATCCTGCCTGATCTGGTGGCCAGCATCGGCGGCCTGATCGTGAA
TTACCTGGAGTGGGTGCAGGATGCCAGCAACTTCTTCTGGACCCCTGAGGAGATCGAGCGGGCCATTGACCTGCGTGTGG
ACGCAGCCGTCAAGAGCGTGATGGCCTTTATGCGCACCCACGACGTGGACATGCGCACGGCGGCCTATGCCCTCGCCCTG
ACCCGCTTGCACCAAGCCACGGTGATGCGCGGCGTCTATCCGTAA

Upstream 100 bases:

>100_bases
CTGAGCGTATCCTGCACCGGGCAGGTCACCTGTCTATGCGTGTATGCTCGGGGAGCCCGACACCCAGCACTTCCCGGTAC
CGCCCCAAAGGAGAAACGCG

Downstream 100 bases:

>100_bases
GGGAGCAGAGGGTGAGGCCGCCGGGCACAGTCGGAACCTCTCCCTCCCCACCACCCGGCCCAACTTCTCCTGACCACACG
CCCCAACTCCTCTCCCGGAG

Product: Glu/Leu/Phe/Val dehydrogenase, dimerisation region

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRASGLNWQGLMEQLQQALPYSEVSDQSLAYFKYPKRTLSVNLPVRMDDGTVRVFKGYRTVHSTARGPSMGGVRFKPGLN
AHECEVLAAIMTLKAAVADLPLGGAKGGVDVDPQQLSPHELEGLTRRYTSELVELVGPSEDILAPDVGTSPQVMAWILDT
YGENTGSTSSGMVVGKPLSLGGSYGSKDARGRSAALVTARVLEARGESLQSARVAVYGFGDVGRRAAQTLAAQGALVIAV
SDQNGATFASSGLDLDALSSYREAHGSVQGFATDITPEEVIELDVDVLMLAYDYGSVNAGNAHAVRARFVVEAANRAVLP
EAERFLQAQGVTILPDLVASIGGLIVNYLEWVQDASNFFWTPEEIERAIDLRVDAAVKSVMAFMRTHDVDMRTAAYALAL
TRLHQATVMRGVYP

Sequences:

>Translated_414_residues
MRASGLNWQGLMEQLQQALPYSEVSDQSLAYFKYPKRTLSVNLPVRMDDGTVRVFKGYRTVHSTARGPSMGGVRFKPGLN
AHECEVLAAIMTLKAAVADLPLGGAKGGVDVDPQQLSPHELEGLTRRYTSELVELVGPSEDILAPDVGTSPQVMAWILDT
YGENTGSTSSGMVVGKPLSLGGSYGSKDARGRSAALVTARVLEARGESLQSARVAVYGFGDVGRRAAQTLAAQGALVIAV
SDQNGATFASSGLDLDALSSYREAHGSVQGFATDITPEEVIELDVDVLMLAYDYGSVNAGNAHAVRARFVVEAANRAVLP
EAERFLQAQGVTILPDLVASIGGLIVNYLEWVQDASNFFWTPEEIERAIDLRVDAAVKSVMAFMRTHDVDMRTAAYALAL
TRLHQATVMRGVYP
>Mature_414_residues
MRASGLNWQGLMEQLQQALPYSEVSDQSLAYFKYPKRTLSVNLPVRMDDGTVRVFKGYRTVHSTARGPSMGGVRFKPGLN
AHECEVLAAIMTLKAAVADLPLGGAKGGVDVDPQQLSPHELEGLTRRYTSELVELVGPSEDILAPDVGTSPQVMAWILDT
YGENTGSTSSGMVVGKPLSLGGSYGSKDARGRSAALVTARVLEARGESLQSARVAVYGFGDVGRRAAQTLAAQGALVIAV
SDQNGATFASSGLDLDALSSYREAHGSVQGFATDITPEEVIELDVDVLMLAYDYGSVNAGNAHAVRARFVVEAANRAVLP
EAERFLQAQGVTILPDLVASIGGLIVNYLEWVQDASNFFWTPEEIERAIDLRVDAAVKSVMAFMRTHDVDMRTAAYALAL
TRLHQATVMRGVYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=344, Percent_Identity=35.1744186046512, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI4885281, Length=344, Percent_Identity=35.1744186046512, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1788059, Length=367, Percent_Identity=28.8828337874659, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17544676, Length=346, Percent_Identity=32.6589595375723, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319256, Length=382, Percent_Identity=25.9162303664921, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324951, Length=420, Percent_Identity=24.5238095238095, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24649283, Length=384, Percent_Identity=30.46875, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI45549226, Length=334, Percent_Identity=32.6347305389222, Blast_Score=205, Evalue=6e-53,
Organism=Drosophila melanogaster, GI45553475, Length=334, Percent_Identity=32.6347305389222, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24649460, Length=282, Percent_Identity=32.2695035460993, Blast_Score=165, Evalue=4e-41,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 44338; Mature: 44338

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASGLNWQGLMEQLQQALPYSEVSDQSLAYFKYPKRTLSVNLPVRMDDGTVRVFKGYRT
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCEEECCCEEEEEEHHHH
VHSTARGPSMGGVRFKPGLNAHECEVLAAIMTLKAAVADLPLGGAKGGVDVDPQQLSPHE
HHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHH
LEGLTRRYTSELVELVGPSEDILAPDVGTSPQVMAWILDTYGENTGSTSSGMVVGKPLSL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
GGSYGSKDARGRSAALVTARVLEARGESLQSARVAVYGFGDVGRRAAQTLAAQGALVIAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEE
SDQNGATFASSGLDLDALSSYREAHGSVQGFATDITPEEVIELDVDVLMLAYDYGSVNAG
ECCCCCEEECCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCEEEEEEEECCCCCCC
NAHAVRARFVVEAANRAVLPEAERFLQAQGVTILPDLVASIGGLIVNYLEWVQDASNFFW
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPEEIERAIDLRVDAAVKSVMAFMRTHDVDMRTAAYALALTRLHQATVMRGVYP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRASGLNWQGLMEQLQQALPYSEVSDQSLAYFKYPKRTLSVNLPVRMDDGTVRVFKGYRT
CCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEECCEEECCCEEEEEEHHHH
VHSTARGPSMGGVRFKPGLNAHECEVLAAIMTLKAAVADLPLGGAKGGVDVDPQQLSPHE
HHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHH
LEGLTRRYTSELVELVGPSEDILAPDVGTSPQVMAWILDTYGENTGSTSSGMVVGKPLSL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
GGSYGSKDARGRSAALVTARVLEARGESLQSARVAVYGFGDVGRRAAQTLAAQGALVIAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEE
SDQNGATFASSGLDLDALSSYREAHGSVQGFATDITPEEVIELDVDVLMLAYDYGSVNAG
ECCCCCEEECCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCEEEEEEEECCCCCCC
NAHAVRARFVVEAANRAVLPEAERFLQAQGVTILPDLVASIGGLIVNYLEWVQDASNFFW
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPEEIERAIDLRVDAAVKSVMAFMRTHDVDMRTAAYALALTRLHQATVMRGVYP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]