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The map label for this gene is gdhA [H]

Identifier: 94984601

GI number: 94984601

Start: 511711

End: 513033

Strand: Reverse

Name: gdhA [H]

Synonym: Dgeo_0494

Alternate gene names: 94984601

Gene position: 513033-511711 (Counterclockwise)

Preceding gene: 94984602

Following gene: 94984599

Centisome position: 20.79

GC content: 65.0

Gene sequence:

>1323_bases
ATGACCACCACGCAGGACCCTAAGAACCAAGAGCAACAGCACCTCAACCTGCACGATCTGCCCAGCTACCTCGACCCGAA
CAACCTGGGGCCGTGGGGCATTTTTCTGGAACAGGTGGAGCGAGTCACGCCCTACCTGGGCAAGCTGGCGTACTGGGTCG
ACACCCTCAAGCGGCCCAAGCGCATCCTGATCGTGGATGTGCCGATCCACCTGGACGACGGCTCGGTGGCTCACTTCGAA
GGCTACCGAGTGCAGCACAACACCTCGCGCGGCCCGGCCAAGGGCGGCGTTCGCTACCACCAGGACGTGACGCTCTCGGA
AGTGATGGCGCTCTCGGCCTGGATGACCATCAAAAACGCCGCCGTCGGTCTCCCCTATGGCGGCGGCAAGGGGGGCATCC
GGATCGATCCGCGCAAGTACAGCCAGGGCGAACTCGAACGGCTGACCCGGCGCTACACCTCCGAGATCGGCTTGATCATT
GGGCCGGAAAAAGACATCCCCGCGCCGGACGTGAACACCAATCCGCAGACGATGGCCTGGATGATGGACACCTACAGCAT
GAATGTGGGCCGGACGGCGACCGGAGTGGTGACCGGCAAGCCGGTGAGCCTGGGGGGCAGCCTGGGCCGCAGTGACGCAA
CCGGACGCGGGGTGTTTGTGACCGGGGCTGAGGCGATGAAAAAGCTGGGGGTACCGCTGGAAGGCGCGCGGATTGCGGTG
CAGGGCTTCGGGAATGTGGGGAGTGCCGCGGCGCGGATCTTCCACGAGCACGGGGCGAAGATCGTGGCGATCCAGGACGT
GAGCGGAACGGTCTACAGCGCGGCGGGGATCGATCCCGCTCAGGCCCTCAAGCAGCTGCAGCAGACCGGCAAGATCACGG
ACCTGGCCGGAACCGAAACCCTGAAGCGTGAGGAATTCTGGAGCGTAGACTGCGACGTCTTGATCCCCGCCGCCCTGGAA
AAGCAGATCACCGAGGCCAACGCCGGACAGATCCAAGCCCGGTTGATCGTGGAGGGGGCCAACGGACCCACCACGCCCCA
GGCCGATGACATCCTGCGCGAACGGGGAGTCACGGTGGTGCCGGACGTGCTGGCCAATGCCGGTGGGGTGACGGTCTCAT
ACTTCGAGTGGGTGCAGGACTTCTCCAGCTTCTTTTGGACCGAGGAGGAGATCAACGCCCGGCTCGACCGCATCATGCGC
GAGGCCTTCCTGAGCCTCTGGGAGGTCAAGGAACGCCACGAGGTGACCTTGCGCACGGCGGCCTATATCGTCGCCTGCAC
CCGCGTCCTCGAAGCCCGCGCCCTGCGTGGCCTCTATCCCTGA

Upstream 100 bases:

>100_bases
GAGGGTGAGGCCGCCGGGCACAGTCGGAACCTCTCCCTCCCCACCACCCGGCCCAACTTCTCCTGACCACACGCCCCAAC
TCCTCTCCCGGAGGCTCCCA

Downstream 100 bases:

>100_bases
GTCAGCAGCCTGCACGCAGGAACAGGGGGAAGCCTCAGCCCAAGCTGGGGCTTCTTTGTGTTGGAGGCCAAGTCCGGCTC
AACTGTTTCGGTCCTTCAGA

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTTTQDPKNQEQQHLNLHDLPSYLDPNNLGPWGIFLEQVERVTPYLGKLAYWVDTLKRPKRILIVDVPIHLDDGSVAHFE
GYRVQHNTSRGPAKGGVRYHQDVTLSEVMALSAWMTIKNAAVGLPYGGGKGGIRIDPRKYSQGELERLTRRYTSEIGLII
GPEKDIPAPDVNTNPQTMAWMMDTYSMNVGRTATGVVTGKPVSLGGSLGRSDATGRGVFVTGAEAMKKLGVPLEGARIAV
QGFGNVGSAAARIFHEHGAKIVAIQDVSGTVYSAAGIDPAQALKQLQQTGKITDLAGTETLKREEFWSVDCDVLIPAALE
KQITEANAGQIQARLIVEGANGPTTPQADDILRERGVTVVPDVLANAGGVTVSYFEWVQDFSSFFWTEEEINARLDRIMR
EAFLSLWEVKERHEVTLRTAAYIVACTRVLEARALRGLYP

Sequences:

>Translated_440_residues
MTTTQDPKNQEQQHLNLHDLPSYLDPNNLGPWGIFLEQVERVTPYLGKLAYWVDTLKRPKRILIVDVPIHLDDGSVAHFE
GYRVQHNTSRGPAKGGVRYHQDVTLSEVMALSAWMTIKNAAVGLPYGGGKGGIRIDPRKYSQGELERLTRRYTSEIGLII
GPEKDIPAPDVNTNPQTMAWMMDTYSMNVGRTATGVVTGKPVSLGGSLGRSDATGRGVFVTGAEAMKKLGVPLEGARIAV
QGFGNVGSAAARIFHEHGAKIVAIQDVSGTVYSAAGIDPAQALKQLQQTGKITDLAGTETLKREEFWSVDCDVLIPAALE
KQITEANAGQIQARLIVEGANGPTTPQADDILRERGVTVVPDVLANAGGVTVSYFEWVQDFSSFFWTEEEINARLDRIMR
EAFLSLWEVKERHEVTLRTAAYIVACTRVLEARALRGLYP
>Mature_439_residues
TTTQDPKNQEQQHLNLHDLPSYLDPNNLGPWGIFLEQVERVTPYLGKLAYWVDTLKRPKRILIVDVPIHLDDGSVAHFEG
YRVQHNTSRGPAKGGVRYHQDVTLSEVMALSAWMTIKNAAVGLPYGGGKGGIRIDPRKYSQGELERLTRRYTSEIGLIIG
PEKDIPAPDVNTNPQTMAWMMDTYSMNVGRTATGVVTGKPVSLGGSLGRSDATGRGVFVTGAEAMKKLGVPLEGARIAVQ
GFGNVGSAAARIFHEHGAKIVAIQDVSGTVYSAAGIDPAQALKQLQQTGKITDLAGTETLKREEFWSVDCDVLIPAALEK
QITEANAGQIQARLIVEGANGPTTPQADDILRERGVTVVPDVLANAGGVTVSYFEWVQDFSSFFWTEEEINARLDRIMRE
AFLSLWEVKERHEVTLRTAAYIVACTRVLEARALRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=376, Percent_Identity=43.3510638297872, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI4885281, Length=340, Percent_Identity=45.2941176470588, Blast_Score=295, Evalue=5e-80,
Organism=Escherichia coli, GI1788059, Length=378, Percent_Identity=34.3915343915344, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17544676, Length=369, Percent_Identity=41.1924119241192, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319256, Length=375, Percent_Identity=31.2, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324951, Length=375, Percent_Identity=30.6666666666667, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45553475, Length=361, Percent_Identity=41.8282548476454, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI45549226, Length=353, Percent_Identity=42.4929178470255, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24649283, Length=331, Percent_Identity=44.7129909365559, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24649460, Length=300, Percent_Identity=42.3333333333333, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 48149; Mature: 48018

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTQDPKNQEQQHLNLHDLPSYLDPNNLGPWGIFLEQVERVTPYLGKLAYWVDTLKRPK
CCCCCCCCCHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RILIVDVPIHLDDGSVAHFEGYRVQHNTSRGPAKGGVRYHQDVTLSEVMALSAWMTIKNA
EEEEEEEEEEECCCCEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHC
AVGLPYGGGKGGIRIDPRKYSQGELERLTRRYTSEIGLIIGPEKDIPAPDVNTNPQTMAW
EEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEE
MMDTYSMNVGRTATGVVTGKPVSLGGSLGRSDATGRGVFVTGAEAMKKLGVPLEGARIAV
EEEHHCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCEEEE
QGFGNVGSAAARIFHEHGAKIVAIQDVSGTVYSAAGIDPAQALKQLQQTGKITDLAGTET
EECCCHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCEEECCCCHH
LKREEFWSVDCDVLIPAALEKQITEANAGQIQARLIVEGANGPTTPQADDILRERGVTVV
HHHHHHCCCCCCEECHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEE
PDVLANAGGVTVSYFEWVQDFSSFFWTEEEINARLDRIMREAFLSLWEVKERHEVTLRTA
CHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYIVACTRVLEARALRGLYP
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTTQDPKNQEQQHLNLHDLPSYLDPNNLGPWGIFLEQVERVTPYLGKLAYWVDTLKRPK
CCCCCCCCHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RILIVDVPIHLDDGSVAHFEGYRVQHNTSRGPAKGGVRYHQDVTLSEVMALSAWMTIKNA
EEEEEEEEEEECCCCEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHC
AVGLPYGGGKGGIRIDPRKYSQGELERLTRRYTSEIGLIIGPEKDIPAPDVNTNPQTMAW
EEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEE
MMDTYSMNVGRTATGVVTGKPVSLGGSLGRSDATGRGVFVTGAEAMKKLGVPLEGARIAV
EEEHHCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCEEEE
QGFGNVGSAAARIFHEHGAKIVAIQDVSGTVYSAAGIDPAQALKQLQQTGKITDLAGTET
EECCCHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCEEECCCCHH
LKREEFWSVDCDVLIPAALEKQITEANAGQIQARLIVEGANGPTTPQADDILRERGVTVV
HHHHHHCCCCCCEECHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEE
PDVLANAGGVTVSYFEWVQDFSSFFWTEEEINARLDRIMREAFLSLWEVKERHEVTLRTA
CHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYIVACTRVLEARALRGLYP
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]