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The map label for this gene is amaA [H]

Identifier: 94984316

GI number: 94984316

Start: 193407

End: 194600

Strand: Reverse

Name: amaA [H]

Synonym: Dgeo_0208

Alternate gene names: 94984316

Gene position: 194600-193407 (Counterclockwise)

Preceding gene: 94984319

Following gene: 94984315

Centisome position: 7.89

GC content: 66.42

Gene sequence:

>1194_bases
ATGACCCAAACGGTGGACCCGGTGGCGACCCTCCGTGACCAGCTCATCGCGTGGCGGCGGCACCTGCACATGCATCCAGA
AGTGGGCTTTGAGGAACACGAGACGGCAGCCTACATCGAGGCCGAGCTGCGGAAGATGCCGGGCCTGACCGTCACCCGCC
CCACCGCGACCAGCGTCCTGGCCGTTCTCAAAGGCGGCCGGCCCGGGCGCACCATTCTGCTGCGGGCCGACATCGATGCC
CTCCCCATCGAGGAGGAGAACACCTTCGAGTTCCGCTCGCGCAGACCCGGGGTCATGCACGCCTGCGGCCACGACGGCCA
TACGGCAATCCTGCTGGGAGTGGCCAAACTGCTCTCCGAACACCCCGAACACGTGCCGGGGGAGGTCCGGATGATCTTCC
AGCATGCCGAGGAGATCGGGCCGGGCGGCGCCGAGGAACTGGTGATGAATACGGGGTTGATGGACGGCGTGGACGTGGTG
ACCGGCCTGCACCTCAACAGCCAACTTCCAACCGGAATGGTGAGTGTCAAGCCGGGGGCCTTTATGGCCGCGCCCGACAG
CCTATACCTCACCATCCAGGGCAAGGGCGGGCACGGCGCCCACCCCGAACAGACGGTGGACCCCATTGCGGTCGGCGCGC
AGGTCGTGACCAACCTCCAGCATGTGGTCAGCCGCCACGTGGCGGCGCTCGACGCTCTGGTCGTCAGCATCACGTCCTTC
CAGAGCGGCACCACCCACAACGTGATTCCCGATACGGCGGTCCTCCAGGGCACCGTGCGGACCTTTGACCCAGAGCTGCG
CCAGCGCGCCCCAAAGCTGATCGAACGCGTGATCAAGGGGGTGTGTGAGGCACATGGGGCGAGCTACACGCTGAAGTACG
AGTTCGGTTACCGGCCCGTCATCAACACCGACTGGGTGGCCGCACAGCTGAGAGAAATCGCGCTGGAGACCGTAGGGGAA
GAGCACTACCAGGACGCGCAGCCCACGATGGGTGGCGAGGATTTCAGCGCCTATCTGGAAAAGGCCCCCGGCGCGTATTT
CAACGTGGGGTCCGGCAGTGACGAGCAGGACAGCCGCTGGCCGCACCACCACCCCCGCTTCACCCTCGACGAGGCCAGCC
TGGAAACCGGGGTCCGGATGCTGCATGCGGCTGCGCTGCGCCTCACGCTGCCGCAGCCGCACCCGCAAGGATAA

Upstream 100 bases:

>100_bases
TCAGCTTGCCCCGCACGCCAAGCAGGTGCTCGTGGGCAGTGCGGCGGCATGGCAGTGGGGCGGACGCGCAGACCCAGGAG
GGTGGGGCGTAGACTGAGGC

Downstream 100 bases:

>100_bases
GGCCATGCTGCCCGAGGCCCTCGCCCGCCAAATCATCACGGACCACAGCCAGCACCCGCGCGGCCGGGGGGAGATCGCGG
GTGTGCCCCACGCCACGCGC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTQTVDPVATLRDQLIAWRRHLHMHPEVGFEEHETAAYIEAELRKMPGLTVTRPTATSVLAVLKGGRPGRTILLRADIDA
LPIEEENTFEFRSRRPGVMHACGHDGHTAILLGVAKLLSEHPEHVPGEVRMIFQHAEEIGPGGAEELVMNTGLMDGVDVV
TGLHLNSQLPTGMVSVKPGAFMAAPDSLYLTIQGKGGHGAHPEQTVDPIAVGAQVVTNLQHVVSRHVAALDALVVSITSF
QSGTTHNVIPDTAVLQGTVRTFDPELRQRAPKLIERVIKGVCEAHGASYTLKYEFGYRPVINTDWVAAQLREIALETVGE
EHYQDAQPTMGGEDFSAYLEKAPGAYFNVGSGSDEQDSRWPHHHPRFTLDEASLETGVRMLHAAALRLTLPQPHPQG

Sequences:

>Translated_397_residues
MTQTVDPVATLRDQLIAWRRHLHMHPEVGFEEHETAAYIEAELRKMPGLTVTRPTATSVLAVLKGGRPGRTILLRADIDA
LPIEEENTFEFRSRRPGVMHACGHDGHTAILLGVAKLLSEHPEHVPGEVRMIFQHAEEIGPGGAEELVMNTGLMDGVDVV
TGLHLNSQLPTGMVSVKPGAFMAAPDSLYLTIQGKGGHGAHPEQTVDPIAVGAQVVTNLQHVVSRHVAALDALVVSITSF
QSGTTHNVIPDTAVLQGTVRTFDPELRQRAPKLIERVIKGVCEAHGASYTLKYEFGYRPVINTDWVAAQLREIALETVGE
EHYQDAQPTMGGEDFSAYLEKAPGAYFNVGSGSDEQDSRWPHHHPRFTLDEASLETGVRMLHAAALRLTLPQPHPQG
>Mature_396_residues
TQTVDPVATLRDQLIAWRRHLHMHPEVGFEEHETAAYIEAELRKMPGLTVTRPTATSVLAVLKGGRPGRTILLRADIDAL
PIEEENTFEFRSRRPGVMHACGHDGHTAILLGVAKLLSEHPEHVPGEVRMIFQHAEEIGPGGAEELVMNTGLMDGVDVVT
GLHLNSQLPTGMVSVKPGAFMAAPDSLYLTIQGKGGHGAHPEQTVDPIAVGAQVVTNLQHVVSRHVAALDALVVSITSFQ
SGTTHNVIPDTAVLQGTVRTFDPELRQRAPKLIERVIKGVCEAHGASYTLKYEFGYRPVINTDWVAAQLREIALETVGEE
HYQDAQPTMGGEDFSAYLEKAPGAYFNVGSGSDEQDSRWPHHHPRFTLDEASLETGVRMLHAAALRLTLPQPHPQG

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=432, Percent_Identity=26.6203703703704, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43276; Mature: 43144

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTVDPVATLRDQLIAWRRHLHMHPEVGFEEHETAAYIEAELRKMPGLTVTRPTATSVL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHH
AVLKGGRPGRTILLRADIDALPIEEENTFEFRSRRPGVMHACGHDGHTAILLGVAKLLSE
HHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
HPEHVPGEVRMIFQHAEEIGPGGAEELVMNTGLMDGVDVVTGLHLNSQLPTGMVSVKPGA
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCEECCCCCCCEEEECCCC
FMAAPDSLYLTIQGKGGHGAHPEQTVDPIAVGAQVVTNLQHVVSRHVAALDALVVSITSF
EEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSGTTHNVIPDTAVLQGTVRTFDPELRQRAPKLIERVIKGVCEAHGASYTLKYEFGYRPV
CCCCCCCCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
INTDWVAAQLREIALETVGEEHYQDAQPTMGGEDFSAYLEKAPGAYFNVGSGSDEQDSRW
CCCHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCC
PHHHPRFTLDEASLETGVRMLHAAALRLTLPQPHPQG
CCCCCCEEECHHHHHHHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure 
TQTVDPVATLRDQLIAWRRHLHMHPEVGFEEHETAAYIEAELRKMPGLTVTRPTATSVL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHH
AVLKGGRPGRTILLRADIDALPIEEENTFEFRSRRPGVMHACGHDGHTAILLGVAKLLSE
HHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHC
HPEHVPGEVRMIFQHAEEIGPGGAEELVMNTGLMDGVDVVTGLHLNSQLPTGMVSVKPGA
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCEECCCCCCCEEEECCCC
FMAAPDSLYLTIQGKGGHGAHPEQTVDPIAVGAQVVTNLQHVVSRHVAALDALVVSITSF
EEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSGTTHNVIPDTAVLQGTVRTFDPELRQRAPKLIERVIKGVCEAHGASYTLKYEFGYRPV
CCCCCCCCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
INTDWVAAQLREIALETVGEEHYQDAQPTMGGEDFSAYLEKAPGAYFNVGSGSDEQDSRW
CCCHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCC
PHHHPRFTLDEASLETGVRMLHAAALRLTLPQPHPQG
CCCCCCEEECHHHHHHHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]