Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 94994176

GI number: 94994176

Start: 754188

End: 755051

Strand: Direct

Name: thyA [H]

Synonym: MGAS10750_Spy0780

Alternate gene names: 94994176

Gene position: 754188-755051 (Clockwise)

Preceding gene: 94994173

Following gene: 94994177

Centisome position: 38.93

GC content: 34.84

Gene sequence:

>864_bases
ATGCTTACAGAAAGGTTATTAGAGATGACAAAAGCTGACCAGATTTTTAAAGCTAATATTCAAAAAATTATAAATGAAGG
ATCATTGAGTGAGCAAGCACGTCCTAAATATAAAGATGGTCGAACAGCTCATTCTAAGTATATTACTGGGGCGTTTGCTG
AATATGATTTAGCTAAAGGAGAGTTTCCCATCACAACTTTACGACCTATTCCAATTAAATCTGCTATAAAGGAATTATTT
TGGATATATCAAGATCAGTCTAACAGTTTAGATGTGCTAGAAGCTAAATATAATGTTCATTATTGGAATGAATGGGAAGT
TGACCAGACACGTACTATTGGGCAACGTTATGGTGCTGTAGTCAAAAAACATGATATCATCTCAAAAATACTAAAGCAAT
TAGCAGAAAATCCTTGGAATCGTCGTAATGTCATTTCTCTCTGGGACTATGAAGCATTTGAGGAGACAAAAGGGTTGTTG
CCATGTGCCTTTCAAATCATGTTTGATGTTAGACGTGTAGGGGAAGATCTTTATTTAGATGCTAGTTTGACGCAACGTTC
TAATGACATATTAGTTGCTCATCACATCAATGCCATGCAATACGTTGCCTTGCAGATGATGATTGCTAAACATTTTGGAT
GGAAAATAGGTAAGTTCTTTTATTTTGTTAACAATTTACATATTTATGACAACCAATTTGATCAGGCTCAAGAATTACTA
AAACGACAGCCAGTTGCCAGTCAGCCTAAGCTTGTTTTAAATGTTCCAGATGGGACTAATTTCTTCGATATTAAACCAGA
TGATTTTGAATTACAAAATTATGATCCAGTGAAGCCTCAGTTGCACTTTGACCTTGCTATTTAA

Upstream 100 bases:

>100_bases
TTATCCTTTAATAGTTAAAATGTTCATGACCACATGAAGTTTTTCGAATGTTTATTTATTCTGGTATTTTTTGGTAAAAT
TAGTTAGAATAGTATGAAGT

Downstream 100 bases:

>100_bases
ACTTTATTTAGATTTTTTGTGAGCATTTTGTTCATAAATTTGGTAAAATAAATAGAGTTTAAAATGAAGGAAATAATTAA
TGACAAAGGAAATTATTGCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLTERLLEMTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLRPIPIKSAIKELF
WIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLL
PCAFQIMFDVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELL
KRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFDLAI

Sequences:

>Translated_287_residues
MLTERLLEMTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLRPIPIKSAIKELF
WIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLL
PCAFQIMFDVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELL
KRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFDLAI
>Mature_287_residues
MLTERLLEMTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKGEFPITTLRPIPIKSAIKELF
WIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLL
PCAFQIMFDVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELL
KRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFDLAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=23.1316725978648, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789191, Length=266, Percent_Identity=30.0751879699248, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71993377, Length=297, Percent_Identity=25.5892255892256, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324648, Length=168, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137556, Length=268, Percent_Identity=25.7462686567164, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 33551; Mature: 33551

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTERLLEMTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
EFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAV
CCCCEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHH
VKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMFDVRRVGEDLYLD
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEE
ASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELL
CHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHEEEEEEECCCHHHHHHHH
KRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFDLAI
HHCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MLTERLLEMTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAFAEYDLAKG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
EFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQTRTIGQRYGAV
CCCCEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHH
VKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMFDVRRVGEDLYLD
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEE
ASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNLHIYDNQFDQAQELL
CHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHEEEEEEECCCHHHHHHHH
KRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFDLAI
HHCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]