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The map label for this gene is dyr [H]

Identifier: 94994177

GI number: 94994177

Start: 755131

End: 755628

Strand: Direct

Name: dyr [H]

Synonym: MGAS10750_Spy0781

Alternate gene names: 94994177

Gene position: 755131-755628 (Clockwise)

Preceding gene: 94994176

Following gene: 94994178

Centisome position: 38.98

GC content: 36.95

Gene sequence:

>498_bases
ATGACAAAGGAAATTATTGCTATTTGGGCAGAGGATGAAGCAGGGCTTATTGGTGTTGCGGGTAAGTTACCATGGTATTT
ACCAAAAGAGTTAGAACATTTTAAAAAGACAACGCTACATCAAGCAATCTTAATGGGCCGTGTGACTTTTGAAGGAATGA
ATTGCAAAAGACTTCCTCAACGTCAAACCCTTGTCATGACAAGAAATAGAGATTATCAAGTAGATGAGGTGTTGACAATG
ACTAGTATTGAAAAAGTCTTGGAGTGGTACCATGCGCAGGATAAAACACTTTATATCATTGGTGGGAATAAGGTTTTAGA
AGCGTTTAATGGTTATTTCGATCGTATCATAAAAACAGTTATCCATCATCGTTTTAAAGGAGATACCTACCGACCACATC
TAGATTTTAGTCATTTCACGCAAGAAAGCCAAACGTTCTATGCTCGAGACGCTAAAAATCCTTATGATTTTACCGTTACT
GCTTTAAAACACAAATAG

Upstream 100 bases:

>100_bases
CACTTTGACCTTGCTATTTAAACTTTATTTAGATTTTTTGTGAGCATTTTGTTCATAAATTTGGTAAAATAAATAGAGTT
TAAAATGAAGGAAATAATTA

Downstream 100 bases:

>100_bases
TCTGAGGAGATGATAAGCGATGCAACGTAGTATTTTTGGAGTATTTACGGCTTTTTTGTGCGTGATTTGTGTTCTTTGTG
CCATCCCAGCTTTTAAAAAG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAILMGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTM
TSIEKVLEWYHAQDKTLYIIGGNKVLEAFNGYFDRIIKTVIHHRFKGDTYRPHLDFSHFTQESQTFYARDAKNPYDFTVT
ALKHK

Sequences:

>Translated_165_residues
MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAILMGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTM
TSIEKVLEWYHAQDKTLYIIGGNKVLEAFNGYFDRIIKTVIHHRFKGDTYRPHLDFSHFTQESQTFYARDAKNPYDFTVT
ALKHK
>Mature_164_residues
TKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAILMGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMT
SIEKVLEWYHAQDKTLYIIGGNKVLEAFNGYFDRIIKTVIHHRFKGDTYRPHLDFSHFTQESQTFYARDAKNPYDFTVTA
LKHK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=150, Percent_Identity=26, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19396; Mature: 19264

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAILMGRVTFEGMNCKRLPQ
CCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
RQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGGNKVLEAFNGYFDRIIKTV
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
IHHRFKGDTYRPHLDFSHFTQESQTFYARDAKNPYDFTVTALKHK
HHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAILMGRVTFEGMNCKRLPQ
CCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
RQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGGNKVLEAFNGYFDRIIKTV
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
IHHRFKGDTYRPHLDFSHFTQESQTFYARDAKNPYDFTVTALKHK
HHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371341; 11463916 [H]