Click here to switch to the map view.

The map label for this gene is rutD [H]

Identifier: 94971872

GI number: 94971872

Start: 84668

End: 85501

Strand: Direct

Name: rutD [H]

Synonym: Dgeo_2404

Alternate gene names: 94971872

Gene position: 84668-85501 (Clockwise)

Preceding gene: 94971873

Following gene: 94971871

Centisome position: 14.75

GC content: 71.46

Gene sequence:

>834_bases
ATGACCGGGTCAGCCGCAGCTGTCCGGCGGGTGAGCATTGGGGATCGAGTCCTCGCCTACACCGAGGTTTCACCGCCCCA
TCCCCGTGCGAACGTGCTGTTTCTCGCCTGGCTGGGGGGGTCGCGGTTGGGGTGGCAGCCGGTGGTAGAGGCTCTCGGTG
ACGCGTACCGCGCCCTCGCCCCCGATCACCGCGACACCGGCGATTCGGAGGCGTTCGCGGATGCCTACCTGCTCGCGGAC
CTTGCCGACGATGCCGCCGCCTTTCTGCGGGCCGTGGACGCCGCGCCCGCCTTCGTCGTGGGCCTCAGCATGGGCGGAAT
GGTGGCGCAGCACCTCGCCCTGCGGCACCCGGACCTCGTACGGGGGCTGGTGCTGGTGTCCACCACACCGGGCGGGGCCG
CGTCCACCCCAGCGACCGAACGGGGACGCGCTGCCCTGTTCCTCCCCGCCGACATGGAGCCGCAGGAGCGGGCCCGGCAG
GCCCTCACGCTGATGACGTATGAGGGGTTTGCGGCCGCTCACCCCGAGGCGCTGGACGTGGCCGCTGCCAACGCTGCCCG
CCACCCCATGAGTGCCGAGAGCTTCAAGCGGCAGTTTCGCGCAATCCGCGCCCACGACACCACTGCCGATCTCGCCCGCC
TCGCCGTCCCCTCTCTGGTGCTGCACGGCGAGCGGGACGACCTGATTCCGCTGCCCAATGCTGAGCGGCTGGCGGCGGGG
ATTCCAGGTGCCGAGCTGCGCGTCTACCCCGCCACCGGCCACATGCCGCACCTCGAACGCCCGGTCCAGTTCCTCCGCGA
CCTGCGCGGCTTTCTAGAGGCGCAGGCGAGCTGA

Upstream 100 bases:

>100_bases
GTTTCTTCGCCACCCACGAGTTGCCGAAGGTGAAGGCCCACGCCGATTTGCTGGCGAGCGCGGACCGAACGACTTTTGAG
ATGCAGGACGCGTGGTTTTG

Downstream 100 bases:

>100_bases
GGCGCTCAGGCTGGTCCGCTGGTATCCGCTGACGTTCAGAATTGACGCGCACGTACATTTCTCCTTATGGTGGGCGGTGA
AGGTTCAAGAGGCTGGCACC

Product: alpha/beta hydrolase fold

Products: 3-oxoadipate

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTGSAAAVRRVSIGDRVLAYTEVSPPHPRANVLFLAWLGGSRLGWQPVVEALGDAYRALAPDHRDTGDSEAFADAYLLAD
LADDAAAFLRAVDAAPAFVVGLSMGGMVAQHLALRHPDLVRGLVLVSTTPGGAASTPATERGRAALFLPADMEPQERARQ
ALTLMTYEGFAAAHPEALDVAAANAARHPMSAESFKRQFRAIRAHDTTADLARLAVPSLVLHGERDDLIPLPNAERLAAG
IPGAELRVYPATGHMPHLERPVQFLRDLRGFLEAQAS

Sequences:

>Translated_277_residues
MTGSAAAVRRVSIGDRVLAYTEVSPPHPRANVLFLAWLGGSRLGWQPVVEALGDAYRALAPDHRDTGDSEAFADAYLLAD
LADDAAAFLRAVDAAPAFVVGLSMGGMVAQHLALRHPDLVRGLVLVSTTPGGAASTPATERGRAALFLPADMEPQERARQ
ALTLMTYEGFAAAHPEALDVAAANAARHPMSAESFKRQFRAIRAHDTTADLARLAVPSLVLHGERDDLIPLPNAERLAAG
IPGAELRVYPATGHMPHLERPVQFLRDLRGFLEAQAS
>Mature_276_residues
TGSAAAVRRVSIGDRVLAYTEVSPPHPRANVLFLAWLGGSRLGWQPVVEALGDAYRALAPDHRDTGDSEAFADAYLLADL
ADDAAAFLRAVDAAPAFVVGLSMGGMVAQHLALRHPDLVRGLVLVSTTPGGAASTPATERGRAALFLPADMEPQERARQA
LTLMTYEGFAAAHPEALDVAAANAARHPMSAESFKRQFRAIRAHDTTADLARLAVPSLVLHGERDDLIPLPNAERLAAGI
PGAELRVYPATGHMPHLERPVQFLRDLRGFLEAQAS

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Escherichia coli, GI1787244, Length=246, Percent_Identity=27.6422764227642, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.24

Molecular weight: Translated: 29483; Mature: 29352

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSAAAVRRVSIGDRVLAYTEVSPPHPRANVLFLAWLGGSRLGWQPVVEALGDAYRALA
CCCCHHHHEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHC
PDHRDTGDSEAFADAYLLADLADDAAAFLRAVDAAPAFVVGLSMGGMVAQHLALRHPDLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCCCHHH
RGLVLVSTTPGGAASTPATERGRAALFLPADMEPQERARQALTLMTYEGFAAAHPEALDV
CCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCHHHHH
AAANAARHPMSAESFKRQFRAIRAHDTTADLARLAVPSLVLHGERDDLIPLPNAERLAAG
HHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCC
IPGAELRVYPATGHMPHLERPVQFLRDLRGFLEAQAS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGSAAAVRRVSIGDRVLAYTEVSPPHPRANVLFLAWLGGSRLGWQPVVEALGDAYRALA
CCCHHHHEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHC
PDHRDTGDSEAFADAYLLADLADDAAAFLRAVDAAPAFVVGLSMGGMVAQHLALRHPDLV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCCCHHH
RGLVLVSTTPGGAASTPATERGRAALFLPADMEPQERARQALTLMTYEGFAAAHPEALDV
CCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCHHHHH
AAANAARHPMSAESFKRQFRAIRAHDTTADLARLAVPSLVLHGERDDLIPLPNAERLAAG
HHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCC
IPGAELRVYPATGHMPHLERPVQFLRDLRGFLEAQAS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxoadipate enol-lactone; H2O

Specific reaction: 3-oxoadipate enol-lactone + H2O = 3-oxoadipate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA