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The map label for this gene is cysJ [H]

Identifier: 94314555

GI number: 94314555

Start: 2395663

End: 2397264

Strand: Reverse

Name: cysJ [H]

Synonym: Rmet_5636

Alternate gene names: 94314555

Gene position: 2397264-2395663 (Counterclockwise)

Preceding gene: 94314556

Following gene: 94314554

Centisome position: 92.91

GC content: 67.73

Gene sequence:

>1602_bases
ATGATGCGTCTTCTACCGTGGGCAATGCTTGTCGCCGGTGTGGCGCTGTCCGTGCTCGCGCACGGTCGCGATGCCATGGC
AGCCGGCGTGGTCATCGCGTACGTTGGCTTCTGCTGGGGCGTGCTCGACGGACATCGCCGCCACCGTGCCGCCCGTGCCG
CGCTTGCCAATGTCGCAAGTGATGACGCGACCGAAGACGCGCATCCGACGCTCGTCGCCTACGCAAGCCAGACCGGCTTT
GCCGAACAGCTCGCCGTGCAAACGGCCCGCGCGTTGCAAGGCGCGGGTCGCCCGGTGCAACTCGTGTCCTTTGCCGAACT
CGATGGTGCGCGTCTGGCTGCGTGCGATCAGGCCCTGTTCCTGGTCAGCACCACGGGTGAGGGCGATGCGCCCGACATGG
CGTCGGGGTTCGCGCGCCGCTTGCTGGCCGGCACCACGCCCGATGCGCTGCGCCAGTTGCGCTATGGCGTCCTTGCGCTG
GGCGATAGCAGCTACGCGCACTTCTGCGCGTTCGGTCGCGCGCTGTCGGGGTGGCTGGAGAGCCATCATGCACAGCCATT
GTTCGATAGGGTCGAGGTCGACAACGGCGACCCCGGCGCGTTGCGCCACTGGCAGAACCACTTGTCCGCGTTGAGCGGCG
GTGCGGAAATCGCGGACTGGGAACGCCCGCGCTATGGCCGCTGGCGGCTGGTGGAGCGCAGGCTGCTCAATCCGGGCAGC
CAGGGCGAGGCTGCCTTTCATCTGGCGCTCGAGCCCGAGGACGCGTCGCAACTGGCATGGCGCGCCGGCGATATTGCCGA
GATTGGCCCATGCCAGCCGCCTGACGTCGTAGACCGGTTGCTGGCTCGCCTGTCGCTCGACGGAACCGCGCCGGTTCGCT
GCGACAGTCGTCCGATGACCTTGTCCGAGGCGTTGGCCACGCGGCTGCTGCTGCCGGATAGCCAGCTGGATATATTGACC
GGATTCACGCCCCAGCGACTGGTCGACGCGTTGCCCGCGCTGCCGCACCGCGAGTACTCGATCGCATCGTTGCCCGCCGA
TGGCCGGCTGGAATTGCTGGTACGTCAGACCCGTCATGCCGATGGGCGGCTTGGTCTCGCCTCTGGCTGGCTGACAGAAT
TCGCGCGCGAGGGCGCGTCGATCGCGCTGCGCGTCCGCACCAATCGCAGCTTCCATCCGCCCGAAGACGGGCGGCCGCTG
ATCCTGATCGGCAACGGTACTGGCCTTGCGGGCCTGCGTGCCGTGATCAAGGCGCGCGCGGAGGCGGGCCATCACCGCAA
CTGGCTGCTGTTCGGCGAGCGCTCCGCTGCGCACGATAACTTCCACGCAGAGGAATTCCAGGCGTGGTTATCGCAGGGCG
TGCTGCAGCGGGTCGACCATGCATGGTCGCGTGATGGCGCGATGCCGCGCTACGTCCAAGACGCCGTGTCCGCGCAGGCT
GCGACGATCGCTGCGTGGGTGGAGCAGGGTGCGTCGATCTACGTTTGCGGCAGCCTGCAGGGCATGGCCGCTGGCGTGCA
GGACGCGCTCCTGCGCATCCTCGGTGAGTCCACACTACAGCGACTGACCGAAGCAGGCAGTTATCGCCGCGACGTGTACT
GA

Upstream 100 bases:

>100_bases
TCACCTTCGCCACGCGCCAGGGCCTGGCTGCCCGCTTCCTGGTGCGCACGCCGCACGGCTTCGATGAGCGGGTCACCCCG
GCGTTCGCCGCGATGCAAAC

Downstream 100 bases:

>100_bases
TGTCGTCGATGTTACGTCGTTGACGCCGTGGAAGCCATCGCGGACGACATGATTTGCGGCGGCGCAGCGTCGCCGGCAGC
GACACATTCCGCTATCCTCG

Product: Sulfite reductase (NADPH) flavoprotein alpha-component

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MMRLLPWAMLVAGVALSVLAHGRDAMAAGVVIAYVGFCWGVLDGHRRHRAARAALANVASDDATEDAHPTLVAYASQTGF
AEQLAVQTARALQGAGRPVQLVSFAELDGARLAACDQALFLVSTTGEGDAPDMASGFARRLLAGTTPDALRQLRYGVLAL
GDSSYAHFCAFGRALSGWLESHHAQPLFDRVEVDNGDPGALRHWQNHLSALSGGAEIADWERPRYGRWRLVERRLLNPGS
QGEAAFHLALEPEDASQLAWRAGDIAEIGPCQPPDVVDRLLARLSLDGTAPVRCDSRPMTLSEALATRLLLPDSQLDILT
GFTPQRLVDALPALPHREYSIASLPADGRLELLVRQTRHADGRLGLASGWLTEFAREGASIALRVRTNRSFHPPEDGRPL
ILIGNGTGLAGLRAVIKARAEAGHHRNWLLFGERSAAHDNFHAEEFQAWLSQGVLQRVDHAWSRDGAMPRYVQDAVSAQA
ATIAAWVEQGASIYVCGSLQGMAAGVQDALLRILGESTLQRLTEAGSYRRDVY

Sequences:

>Translated_533_residues
MMRLLPWAMLVAGVALSVLAHGRDAMAAGVVIAYVGFCWGVLDGHRRHRAARAALANVASDDATEDAHPTLVAYASQTGF
AEQLAVQTARALQGAGRPVQLVSFAELDGARLAACDQALFLVSTTGEGDAPDMASGFARRLLAGTTPDALRQLRYGVLAL
GDSSYAHFCAFGRALSGWLESHHAQPLFDRVEVDNGDPGALRHWQNHLSALSGGAEIADWERPRYGRWRLVERRLLNPGS
QGEAAFHLALEPEDASQLAWRAGDIAEIGPCQPPDVVDRLLARLSLDGTAPVRCDSRPMTLSEALATRLLLPDSQLDILT
GFTPQRLVDALPALPHREYSIASLPADGRLELLVRQTRHADGRLGLASGWLTEFAREGASIALRVRTNRSFHPPEDGRPL
ILIGNGTGLAGLRAVIKARAEAGHHRNWLLFGERSAAHDNFHAEEFQAWLSQGVLQRVDHAWSRDGAMPRYVQDAVSAQA
ATIAAWVEQGASIYVCGSLQGMAAGVQDALLRILGESTLQRLTEAGSYRRDVY
>Mature_533_residues
MMRLLPWAMLVAGVALSVLAHGRDAMAAGVVIAYVGFCWGVLDGHRRHRAARAALANVASDDATEDAHPTLVAYASQTGF
AEQLAVQTARALQGAGRPVQLVSFAELDGARLAACDQALFLVSTTGEGDAPDMASGFARRLLAGTTPDALRQLRYGVLAL
GDSSYAHFCAFGRALSGWLESHHAQPLFDRVEVDNGDPGALRHWQNHLSALSGGAEIADWERPRYGRWRLVERRLLNPGS
QGEAAFHLALEPEDASQLAWRAGDIAEIGPCQPPDVVDRLLARLSLDGTAPVRCDSRPMTLSEALATRLLLPDSQLDILT
GFTPQRLVDALPALPHREYSIASLPADGRLELLVRQTRHADGRLGLASGWLTEFAREGASIALRVRTNRSFHPPEDGRPL
ILIGNGTGLAGLRAVIKARAEAGHHRNWLLFGERSAAHDNFHAEEFQAWLSQGVLQRVDHAWSRDGAMPRYVQDAVSAQA
ATIAAWVEQGASIYVCGSLQGMAAGVQDALLRILGESTLQRLTEAGSYRRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=25.752508361204, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI169790958, Length=257, Percent_Identity=27.2373540856031, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI169790956, Length=257, Percent_Identity=27.2373540856031, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI7657393, Length=217, Percent_Identity=31.7972350230415, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI221316709, Length=215, Percent_Identity=31.1627906976744, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI10835173, Length=225, Percent_Identity=30.6666666666667, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221316705, Length=226, Percent_Identity=30.5309734513274, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI127139033, Length=235, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI40254422, Length=209, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI221316707, Length=196, Percent_Identity=30.1020408163265, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789123, Length=572, Percent_Identity=27.972027972028, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17554134, Length=595, Percent_Identity=24.2016806722689, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17566446, Length=245, Percent_Identity=30.2040816326531, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321143, Length=232, Percent_Identity=25.8620689655172, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325305, Length=216, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24660907, Length=205, Percent_Identity=30.2439024390244, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24660903, Length=205, Percent_Identity=30.2439024390244, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17137192, Length=214, Percent_Identity=28.9719626168224, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582192, Length=214, Percent_Identity=28.9719626168224, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583543, Length=315, Percent_Identity=29.8412698412698, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI78706876, Length=315, Percent_Identity=29.8412698412698, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI78706872, Length=315, Percent_Identity=29.8412698412698, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 57580; Mature: 57580

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLLPWAMLVAGVALSVLAHGRDAMAAGVVIAYVGFCWGVLDGHRRHRAARAALANVAS
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDATEDAHPTLVAYASQTGFAEQLAVQTARALQGAGRPVQLVSFAELDGARLAACDQALF
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCHHHHHCCEEE
LVSTTGEGDAPDMASGFARRLLAGTTPDALRQLRYGVLALGDSSYAHFCAFGRALSGWLE
EEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHH
SHHAQPLFDRVEVDNGDPGALRHWQNHLSALSGGAEIADWERPRYGRWRLVERRLLNPGS
HCCCCCCHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
QGEAAFHLALEPEDASQLAWRAGDIAEIGPCQPPDVVDRLLARLSLDGTAPVRCDSRPMT
CCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCH
LSEALATRLLLPDSQLDILTGFTPQRLVDALPALPHREYSIASLPADGRLELLVRQTRHA
HHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHCCC
DGRLGLASGWLTEFAREGASIALRVRTNRSFHPPEDGRPLILIGNGTGLAGLRAVIKARA
CCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
EAGHHRNWLLFGERSAAHDNFHAEEFQAWLSQGVLQRVDHAWSRDGAMPRYVQDAVSAQA
HCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ATIAAWVEQGASIYVCGSLQGMAAGVQDALLRILGESTLQRLTEAGSYRRDVY
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMRLLPWAMLVAGVALSVLAHGRDAMAAGVVIAYVGFCWGVLDGHRRHRAARAALANVAS
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDATEDAHPTLVAYASQTGFAEQLAVQTARALQGAGRPVQLVSFAELDGARLAACDQALF
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCHHHHHCCEEE
LVSTTGEGDAPDMASGFARRLLAGTTPDALRQLRYGVLALGDSSYAHFCAFGRALSGWLE
EEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHH
SHHAQPLFDRVEVDNGDPGALRHWQNHLSALSGGAEIADWERPRYGRWRLVERRLLNPGS
HCCCCCCHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
QGEAAFHLALEPEDASQLAWRAGDIAEIGPCQPPDVVDRLLARLSLDGTAPVRCDSRPMT
CCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCH
LSEALATRLLLPDSQLDILTGFTPQRLVDALPALPHREYSIASLPADGRLELLVRQTRHA
HHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHCCC
DGRLGLASGWLTEFAREGASIALRVRTNRSFHPPEDGRPLILIGNGTGLAGLRAVIKARA
CCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
EAGHHRNWLLFGERSAAHDNFHAEEFQAWLSQGVLQRVDHAWSRDGAMPRYVQDAVSAQA
HCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ATIAAWVEQGASIYVCGSLQGMAAGVQDALLRILGESTLQRLTEAGSYRRDVY
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA